• Title/Summary/Keyword: Plant collection

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Genetic diversity in kiwifruit germplasm evaluated using RAPD and SRAP markers (RAPD와 SRAP 마커를 이용한 참다래 유전자원의 유전적 다양성)

  • Cho, Kang Hee;Kwack, Yong-Bum;Park, Seo Jun;Kim, Se Hee;Lee, Han Chan;Kim, Mi Young
    • Journal of Plant Biotechnology
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    • v.44 no.3
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    • pp.303-311
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    • 2017
  • In this study, random amplified polymorphic DNA (RAPD) and sequence-related amplified polymorphism (SRAP) analyses were used for evaluation of genetic diversity of 61 kiwifruit (Actinidia spp.) germplasms including domestic and overseas collection cultivars. Forty RAPD primers were detected in a total of 230 polymorphic bands with an average of 5.75. Thirty-two SRAP primer combinations were detected in a total of 204 polymorphic bands with an average 6.38. By unweighted pair-group method arithmetic average cluster analysis using 434 polymorphic bands, kiwifruit germplasms were classified in three groups with similarity value of 0.680. Cluster I consisted of 46 kiwifruit germplasms belonging to A. deliciosa, A. chinensis, A. deliciosa ${\times}$ A. arguta, A. chinensis ${\times}$ A. arguta, and A. chinensis ${\times}$ A. deliciosa. Cluster II consisted of seven germplasms belonging to A. arguta and 'Skinny Green', a cultivar derived from a cross between A. arguta and A. deliciosa. Cluster III consisted of seven germplasms belonging to A. rufa, A. hemsleyana, A. macrosperma, A. polygama, and A. eriantha. Genetic similarity values among tested kiwifruit germplasms ranged from 0.479-0.991, and average similarity value was 0.717. Similarity value was highest (0.991) between NHK0038 (A. deliciosa) and NHK0040 (A. deliciosa), and lowest (0.479) between 'Hayward' (A. deliciosa) and K5-1-22 (A. arguta).

Comparative Analysis of Coat Protein Gene of Isolates of Cucumber mosaic virus Isolated from Pepper Plants in Two GMO Environmental Risk Assessment Fields (GM 격리포장 내 고추에서 분리한 Cucumber mosaic virus 분리주들의 외피단백질 유전자 비교)

  • Hong, Jin-Sung;Park, Ho-Seop;Ryu, Ki-Hyun;Choi, Jang-Kyung
    • Research in Plant Disease
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    • v.15 no.3
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    • pp.165-169
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    • 2009
  • Twelve Cucumber mosaic virus (CMV) isolates were isolated from genetically modified (GM) and non-GM Capsicum annuum in two GM fields, Namyangju and Anseong, and their properties were investigated in this study. Coat protein (CP) gene of the CMV isolates were synthesized by RT-PCR using genus-specific primers which designed to amplify a DNA fragment of 950 bp. Purified cDNA fragments were cloned into the pGEMT easy vector for sequence determination. Nucleotide sequences (internal 657 bp) of CMV isolates were compared with Fny-CMV CP sequences and there were no significant collection site specific sequence similarities found. When predicted amino acid sequences (219 amino acids) were compared with Fny-CMV CP amino acids sequences, there were 96.8% to 97.3% similarities found from Namyangju collections and 95.9% to 96.8% similarities from Anseong collections. The phylogenetic analysis with nucleotide sequences showed definite differences in CMVs which have been isolated from the two regions.

Control of Agrimony (Agrimonia pilosa) Powdery Mildew Using Cooking Oil and Yolk Mixture (난황유를 이용한 짚신나물 흰가루병의 방제)

  • Han, Ki-Soo;Lee, Jung-Han;Kwon, Young-Sang;Bae, Dong-Won;Kim, Dong-Kil;Kim, Hee-Kyu
    • Research in Plant Disease
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    • v.14 no.3
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    • pp.201-204
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    • 2008
  • We've been involved in collection and domestication of Agrimonia pilosa from 2005 to 2007. We observed agrimony powdery mildew caused by Sphaerotheca aphanis. The powdery mildew developed twice a year, in May to June and mid August to September on whole plants of A. pilosa plants, that is, leaves, stem and floral part. We sprayed the Cooking Oil & Yolk mixture (COY) to control the agrimony powdery mildew. When COY was single-sprayed to control agrimony powdery mildew, its control value was 26.9%. However when it was double-sprayed and triple-sprayed at seven days interval, its control value increased up to 80.8 and 82.7%, respectively. Upon the first spray, symptoms of powdery mildew disappeared within three days and suppressive effect of COY was persisted for only seven days. Thereafter, powdery mildew was relapsed to develop. However, on double to triple sprays, powdery mildew was suppressed more than 28 days after final treatment. Therefore, it is concluded that double spray of COY at seven days interval can control effectively agrimony powdery mildew.

Development of the Pushing Type Cutting Device to Dismantle Concrete Structure for Decommissioning of Nuclear Power Plant (원전해체 시 콘크리트 구조물 절단을 위한 밀기형 절단장치 개발)

  • Lee, Bong-Jae;Kwon, Yong-Kyu;Hong, Chang-Dong;Lee, Dong-Won;Min, Kyong-Nam
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.18 no.1
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    • pp.103-111
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    • 2020
  • Pulling-type cutting devices, which use a diamond wire saw, have been used generally for cutting concrete structures. In this study, a pushing-type cutting device with a collection cover was developed by overcoming the disadvantages of pulling-type devices. In this device, dry or liquid methods can be selected to cool frictional heat. Operation and leakage tests of the dust generated during the dismantling of a concrete structure were carried out, confirming the suitable operation of the fabricated cutting device; the leakage rate was approximately 1.7%. For a conservative evaluation, the internal dose of workers was estimated in dismantling the core center part of biological shield concrete with a specific activity of 99.5 Bq·g-1. The committed effective dose per worker was 0.25 mSv. The developed cutting device contributed to reducing radioactive concrete waste and minimizing worker exposure due to its easy installation. Therefore, it can be utilized as a cutting apparatus for dismantling not only reinforced concrete structures but also radioactive biological shield concrete in nuclear power plant decommissioning efforts.

Englishness represented in a Cottage Garden (코티지 가든에 표상된 영국성)

  • Cho, Hye-Ryeong
    • Journal of the Korean Institute of Landscape Architecture
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    • v.45 no.1
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    • pp.63-72
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    • 2017
  • Gardening activities, plant raising, and general flowerbeds the public makes today can be found in the original form of cottage gardens in the United Kingdom. A cottage garden is a popular garden style of modern Britain, implying unique Englishness including ethnic sense and vernacular. In addition, the purpose of this study is to consider the modern movement in the United Kingdom in the past 200 years and read Englishness of cottage gardens through style differentiation and background of occurrence of cottage gardens appearing in this process. Therefore, this study is summarized as follows. First, a view of nature of the Englishman loving freedom and landscape acts as a key part of patriotism and is connected to the preservation of idyllic England. For this ideal of the Englishman of the country, idyllic British characteristics are found in various literatures and artistic fruits; cottage gardens, that is a form of new garden, were made with invigoration of supply and collection of plants. Second, an early form of cottage gardens was the domestic garden, in which there is a vegetable garden by middle-class move to a suburb according to urbanization, but evolved into a form of garden having both artistry and regionality, vernacular, and ecological characteristics with various situations of modern society(handicraft promotion movement, preservation of remains, and ancient building restoration movement). Wild gardens occurring in this process are a type of garden realizing wild fields and forests in the United Kingdom;they have made a big impact on many garden designers up to now. Cottage gardens, reflecting a variety of Englishness, is a subject of city planning and flower shows and is a culture symbolizing the United Kingdom.

The Regional Folk Plants in Southern Inland Area of Gyeonggi-do (경기남부 내륙지역의 민속식물자원)

  • Jeong, Hye-Ran;Choi, Kyung;Park, Kwang-Woo
    • Korean Journal of Plant Resources
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    • v.25 no.5
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    • pp.523-542
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    • 2012
  • This study was carried out to clarify the regional folk plants in the southern inland area of Gyeonggi-do Province. According to the survey results, derived from 1,229 sheets of 90 residents at 35 places from 9 counties and cities between October 2009 and December 2010, the folk plants in the inland southern area of Gyeonggi-do consisted of a total of 259 taxa; 82 families, 200 genera, 221 species, 4 subspecies, 32 varieties, and 2 forma. The classification of the plants by its usage was; 214 as edible, 119 as medicinal, 34 as ornamental, 18 as timber, 12 as dye, 5 as oil, 3 as spice, 2 as fiber, 2 as aroma, and 22 as others uses. So, the edible and medicinal uses are higher than other uses. The most useful part was the leaf, followed by fruit, stem, root, and flower. The collection season was focused on spring and summer, because the leaf was mainly collected in an edible condition during those seasons. Traditional knowledge of folk plants was mostly passed down orally. The consistency comparison between the standard common name and the local name was the highest in the people in 50s and the lowest in those in 80s.

Studies on physio-ecological characteristics of local collections of Monochoria vaginalis Presl. (물달개비(Monochoria vaginalis Presl.)의 지방수집종간(地方蒐集種間) 생리생태적(生理生態的) 특성(特性))

  • Park, K.H.;Kim, K.U.
    • Korean Journal of Weed Science
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    • v.7 no.1
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    • pp.3-11
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    • 1987
  • The study was conducted to identify growth habit of Monochoria vaginalis gathered from three different locations (Suweon, Jeonju and Milyang). The plant height, number of leaves and dry weight of M. vaginalis gathered from three different locations were greatly affected by the seeding dates. The earlier the seeds of M. varginalis sown, the greater M. vaginalis grew, In general, the earlier seeding dates produced significantly longer plant height, number of leaves and dry weight than those of later seeding dates, showing no difference in the collection of weed seeds in different locality. The earlier seeding dates resulted in the earlier flowering, but the interval between dates of seeding and flowering decreased progressively as the seeding times were delayed. Seed production was not significant among three locations and but seeding date on June 15 produced th highest seed production as compared with other seeding times. No. of flowers and capsule per pot among yield components were two major factors affecting seed production.

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A study on morphological and pattern analysis in two kinds of Aconiti Radix (부자(附子)와 초오(草烏)의 내외부형태(內外部形態)와 패턴분석연구)

  • Kang, Gyun-Heok;Choi, Go-Ya;Kim, Hong-Jun;Ju, Young-Sung
    • Korean Journal of Oriental Medicine
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    • v.12 no.1
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    • pp.23-38
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    • 2006
  • The taxonomic list of specific features in external and internal shape and the pattern analysis of Aconitum carmichalei $D_{EBX}$ as the original plant of Aconiti Lateralis Radix Preparata and Aconitum cliiare Dc as the original plant of Aconiti Ciliare Tuber are as follows. 1. Aconitum carmichalei $D_{EBX}$ has tri-palmately parted leaves, petiole in lower leaves, and its ovary has short hair. Whereas Aconitum cliare Dc has $3{\sim}4$ parted leaves, long petiole, and its ovary has not hair. 2. Aconitum carmichalei $D_{EBX}$ has cylinder shape is relatively small in length and diameter, is greyish brown blacky brown in outer surface, greyish $white{\sim}dark$ gray in section. 3. According to the collection place, there is a remarkable difference in the physical shape of herbal states. Aconiti Lateralis Radix Preparate(medicated in Korea) is more transparent blacky brown color than Aconiti Lateralis Fadix Preparata(medicated in Chian). Also Black Aconi Radix(墨附片) has exodermis and White Aconi Radix(白附片) has not. 4. The internal characteristics entirely correspond to in internal shape described in the literatures, Only it is possible to discriminate between black Aconi Radix(墨附片) and White Aconi Radix(白附片) by the existence of cork layer. The classification between Aconiti Lateralis Radix Preparata and Aconiti Ciliare Tuber makes entirely Tuber makes entirely remarkable difference in the physical shape of cambium layer Namely, in shape of cambium layer the kinds of Aconiti lateralis Radix Prepala has horn-like shape and the kinds of Aconiti Ciliare Tuber has circle-like shape. 5. In the peak of examination substance in comparison to Rt of the index material diterpene alkaloid mesaconitine, aconitine, hypaconitine chromatogram Aconiti Ciliare Tuber is higher than in Aconiti Lateralis Radix Preparata This explain that the component changes after the process of medicine. 6. In the Content of mesaconitine, aconitine and hypaconitime Aconiti Ciliare Tuber is higher than Aconiti Lateralis Radix Preparata. 7. In Aconiti Lateralis Radix Preparata, aconitine, hypaconitine and mesaconitine each appears in Rf 0.46, 0.54, 0.32. But except Aconiti Ciliare Tuber the band does not appear. For the future, such results will be used as the basic source of additional research, and a far-reaching comparative study is needed to distinguish between many kinds of same genus-degree of relatedness.

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Analysis of Crop Survey Protocols to Support Parameter Calibration and Verification for Crop Models of Major Vegetables (주요 채소 작물 대상 작물 모형 모수 추정 및 검증을 지원하기 위한 생육 조사 프로토콜 분석)

  • Kim, Kwang Soo;Kim, Junhwan;Hyun, Shinwoo
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.22 no.2
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    • pp.68-78
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    • 2020
  • Crop models have been used to predict vegetable crop yield, which would have a considerable economic impact on consumers as well as producers. A small number of models have been developed to estimate growth and yield of vegetables due to limited availability of growth observation data in high-quality. In this study, we aimed to analyze the protocols designed for collection of the observation data for major vegetable crops including cabbage, radish, garlic, onion and pepper. We also designed the protocols suitable for development and verification of a vegetable crop growth model. In particular, different measures were proposed to improve the existing protocol used by Statistics Korea (KOSTAT) and Rural Development Administration (RDA), which would enhance reliability of parameter estimation for the crop model. It would be advantageous to select sampling sites in areas where reliable weather observation data can be obtained because crop models quantify the response of crop growth to given weather conditions. It is recommended to choose multiple sampling sites where climate conditions would differ. It is crucial to collect time series data for comparison between observed and simulated crop growth and yield. A crop model can be developed to predict actual yield rather than attainable yield using data for crop damage caused by diseases and pests as well as weather anomalies. A bigdata platform where the observation data are to be shared would facilitate the development of crop models for vegetable crops.

Analysis of Nutritional Composition and Phenolic Compound in Propolis Collected from Falseacacia and Chestnut Tree in Korea (국내산 아까시나무와 밤나무 유래 propolis의 영양성분 및 페놀성 화합물 분석)

  • Song, Hyo-Nam;Gil, Bog-Im
    • Korean Journal of Food Science and Technology
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    • v.34 no.4
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    • pp.546-551
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    • 2002
  • Nutritional composition and phenolic compounds of raw propolis collected from falseacacia (Robinia pseudoacacia L.) and chestnut tree (Castanea crenata), and their 70% ethanol extracts of propolis (EEP) were analyzed. Propolis had high crude lipid content, but no significant differences in general compositions in terms of collection area and plant origins. Mineral contents varied greatly depending on the plant origins, with falseacacia propolis showing the highest mineral content. Sixteen amino acids were analyzed, among which aspartic acid content was the highest at $328.4{\sim}410.6\;mg%$ and methionine the lowest at $0{\sim}21.1\;mg%$. Extraction yield for EEP was relatively high at $64.2{\sim}81.9%$, and total polyphenol and flavonoid contents were $13.9{\sim}23.7$ and $8.6{\sim}10.8%$, respectively. HPTLC and HPLC analysis on the phenolic compounds revealed the overall chromatographic patterns were almost equal, showing similar polyphenol compositions between the propolis. About 16 peaks were identified by HPLC analysis, among which 6 peaks of p-hydroxy benzoic acid, caffeic acid, ferulic acid, benzoic acid, cinnamic acid, and chrysin were identified.