• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2020년도 춘계학술대회
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• pp.47-47
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• 2020

The Korean National Agrobiodiversity Center holds the more than 1300 accessions of proso millet, but a large portion of accessions are landrace of Korea that has very similar traits. To comprehend the maximum genetic diversity of this crop, a core collection with minimum number of accessions will facilitate easy access to genetic material. Here we assessed the genetic diversity and population structure in a germplasm collection of 830 accessions by employing EST-SSR markers and morphological traits. A total of 107 alleles were detected with an average allele number of 4.9 per locus among the 830 accessions based on 37 EST-SSR markers. The number of alleles per locus ranged from 2 to 7. Polymorphism information content and expected heterozygosity ranged from 0.06 to 0.68 (mean = 0.21) and 0.06 to 0.73 (mean = 0.23), respectively. The germplasm collection was separated into two groups based on population structure analysis, whereas principal coordinate analysis (PCoA) could not cluster accessions according to their geographic origin. Subsequently, a preliminarily developed core collection with a total of 141 accessions (17%) was selected from the whole set of germplasm by combining allelic variations of EST-SSR markers and eight different phenotypic traits. The core collection optimally represented the whole germplasm collection and displayed a similar level of PCoA value and genetic variation from the initial collection. The results obtained here provide a primary resource for further genetic analysis and establish a reference for further development of appropriate genetic breeding strategies.

• Mycobiology
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• 제31권1호
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• pp.9-18
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• 2003

A total of 82 genera and 271 species of soil-inhabiting fungi including saprobic, nematode-trapping, and arbuscular mycorrhizal but plant pathogenic fungi published hitherto in South Korea are listed with the information on geographic location, habitat, vegetation when available, and relevant literatures.

• 한국자원식물학회지
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• 제11권1호
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• pp.70-79
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• 1998

This study was conducted to understand the effects of weather and tapping date on yield and quality of lactree(Rhus verniciflua) sap yield showed a significant positive correlation with the minimum temperature of one day before sap collection at 1% level and with theminimum humidity of theday of sap collection at 5% level. However, the differences between the maximum and the minimum temperatures and humidities of the day of sap collection were negatively correlated with the sap yield at 5% level. Multiple regression analysis indicated that the minimum temperature of one day bofore sap collection and the minumum humidity of the day sap collection were important factors for increasing sap yield. The high sap yield of lactree by Japanese tapping method was recorded during mid-July and early August. Seasonal variation in lactree sap constituents was observed. The sap collected on 15th of August contained the highest urushiol content (68.3%) and the lowest water content resulting in high quality of lactree sap. By reversed-phase HPLC analysis, fove urushiol components were separated from each other depending on the number of doulbe bonds in the side-chain , and seaxonal variation of urushiol composition was noticed. The 3-C15 triene content fo the sap collected on 5th of July was the highest(77.56%) indicating the major component of urushiol which affects lactree sap quality.

• 식물분류학회지
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• 제49권4호
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• pp.275-281
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• 2019

One key step in the plant restoration process is the collection of seeds from the field. For the selection of source populations of target plant species for translocation purposes (reintroduction or reinforcements), several approaches are possible. A practical method involves the use of data from reciprocal transplant studies. If no direct data are available, knowledge of population genetics and the phylogeography of the target species can serve as an alternative. In this short review, we briefly propose guidelines for those collecting seeds for plant species restoration based on population genetics theory, focusing on two main questions: Where does the plant material come from and how are sources designated, and how are seeds efficiently collected from local populations? While genetic data on a larger scale (phylogeography and population genetics) are needed to form a reply to the first question, similar data on a smaller scale (fine-scale genetic structures within populations) are necessary to shed light on the second issue.

• Current Research on Agriculture and Life Sciences
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• 제32권4호
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• pp.175-177
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• 2014

This is the first report describing the seasonal conditions affecting shoot regeneration by the chrysanthemum cv. Baeksun. The shoot regeneration from petal explants was found to be more favorable from September to December, reaching the highest values in December. In addition, the quality of the shoots was also influenced according to the season of the explant collection, where healthy and uniform plants were derived from the explants collected in December. Choosing the proper season for explant collection affected the successive plant growth parameters (i.e., plant height and fresh weight). Thus, the current results strongly suggest that season plays an important role in plant tissue culturing, which is an essential tool for micropropagation and Agro-bacterium-mediated genetic transformation studies.

• International Journal of Industrial Entomology and Biomaterials
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• 제15권1호
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• pp.23-33
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• 2007

A simple and reliable cryo technique using desiccation and slow freezing of winter dormant buds was employed for 238 core collection of mulberry germplasm collected from diverse geographical regions and maintained under tropical conditions in the ex situ field gene bank to develop long-term biodiversity conservation for ensuring sustainable utilization of these valuable resources. Desiccation and freezing tolerance of bud grafts and excised shoot apices in the axillary buds of different Morus species under in vivo and in vitro condition indicated species-specific variation and most of the wild Morus species were found sensitive. In vitro regeneration and cryopreservation($-196^{\circ}C$) protocols using differentiated bud meristem like axillary winter dormant buds were worked out for a wide range of Morus species, land races, wild and cultivated varieties. Successful cryopreservation of mulberry winter dormant buds of different accessions belonging to M. indica, M. alba, M. latifolia, M. cathayana, M. laevigata, M. nigra, M. australis, M. bombycis, M. sinensis, M multicaulis and M. rotundiloba was achieved. Among wild species Morus tiliaefolia, and M. serrata showed moderate recovery after cryopreservation. Survival rates did not alter after three years of cryopreservation of different Morus species. ISSR markers were used to ascertain the genetic stability of cryopreserved mulberry, which showed no difference detected among the plantlets regenerated from frozen apices in comparison to the non-frozen material.

• 식물분류학회지
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• 제34권1호
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• pp.37-41
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• 2004

T. Nakai는 1911년 Flora Koreana vol. II에 러시아 식물분류학자인 V. L. Komarov의 1897년 북한 채집 표본을 120여점 인용하였다. Nakai가 언급한 북한의 지명은 약 65개로서 Komarov의 채집지명의 상당 부분에 해당된다. Nakai가 언급한 지명은 러시아발음에 근간을 두고 로마자로 표기하였는데 최근 혹은 과거 지도에서 정확한 채집지명을 확인하기가 매우 어렵다. 본 연구는 Flora Koreana II에 기재된 채집지명을 과거 발표된 러시아와 일본 번역서인 만주식물지와 표본레이블 등의 자료를 근간으로 Nakai가 언급한 원명과, 한자 지명, 현재 지명의 로마자표기, 위도와 경도, 출처의 페이지 등을 도표로 제시하여 정리하였다.

• Journal of Ecology and Environment
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• 제35권4호
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• pp.301-306
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• 2012

Determination of the minimum population size is an important component for the ex situ conservation of an endangered species. Here, we present the identification of collection strategies that most efficiently captured the genetic diversity of Brasenia schreberi J.F. Gmelin (water-shield) in natural populations from the mainland (MGC) and Jeju Island (JNS) of South Korea, using amplified fragment length polymorphism (AFLP) markers. A total of 313 and 383 polymorphic bands were detected in the MGC and JNS populations, respectively. All of the 140 sampled ramets were distinguishable by the presence of distinct AFLP phenotypes. According to the simulation of the individual sampling by maximization sampling, 25 and 28 individuals captured all of the genetic diversity in the MGC population (mainland of South Korea) and the JNS population (Jeju Island), respectively. The level of genetic diversity of the core collections was similar to the entire collection, indicating that the core collections very well represent the diversity of the entire collection. We therefore suggest a management unit of B. schreberi based on the genetic information for assessing the minimum population size for its ex situ conservation.

• Journal of Applied Biological Chemistry
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• 제66권
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• pp.227-235
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• 2023

The current study evaluated the presence and concentration of health-promoting phenolic antioxidants in sorghum seed accessions from seven regions in three different countries (Uzbekistan, Myanmar, and the United States). The free radical scavenging activity using the 2,2-diphenyl-1-picrylhydrazyl assay was 13.10±2.37 ㎍/mL in the Uzbekistan sorghum seed extracts, demonstrating the highest antioxidant activity. Additionally, the total phenol content was the highest in the Uzbekistan sorghum seed extracts [157.87±4.04 mg GAE/g (gallic acid equivalents per gram)]. The total flavonoid content was 12.69±0.72 mg QE/g (quercetin equivalents per gram) and represents the highest value in the Myanmar collection. The Uzbekistan collection demonstrated the greatest potential for whitening activity, with the tyrosinase inhibition rate of 79.20±4.15%. The wrinkle improvement function using elastase also showed the highest anti-aging activity of 55.85±4.78% in the same seed extract. The Uzbekistan seed extract had the highest NO production inhibitory activity of 144.35±4.55%. We conclude from these results that the Uzbekistan sorghum seed has excellent antioxidant activity, content of various functional ingredients, and anti-inflammatory activity.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2000년도 봄 학술발표회 논문집
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• pp.153-158
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• 2000

This study was carried out in order to investigate the relationship roundness of fly ashes and fluidity of fly ash cement paste. electrostatic precipitator system in coal-fired power plant have many successive collection fields in the direction of flue gas travel. In experiment, the roundness of fly ashes collected from the same location had similar values regardless of the load or boiler, and they increased going from the 1st collection filed to third collection filed in the direction of gas movement. In considering the relation between roundness of fly ashes and fluidity of fly ash cement paste, it has a high correlation.