• Title/Summary/Keyword: Plant activator

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Analysis of Tissue Plasminogen Activator Expression using Pollen Culture in vitro (기내 화분배양을 이용한 Tissue Plasminogen Activator 발현분석)

  • 박인혜;박희성
    • KSBB Journal
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    • v.17 no.6
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    • pp.582-585
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    • 2002

  • In an effort to use plant biotechnology for the production of biopharmaceuticals, pollens collected from lily (Lilium longiflorum) were grown in vitro and transformed with a PCR-amplified 1.7 kb cDNA encoding human tissue plasminogen activator (tPA) using Agrobacterium via a vacuum infiltration process. Western blotting showed that transgenic lily pollen tubes selected on kanamycin for 16 hrs expressed a tPA protein with a size similar to the human standard, suggesting their possible use as a disposable host for rapid foreign protein production.

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The Potato Transcriptional Co-activator StMBF1 Is Up-regulated in Response to Oxidative Stress and Interacts with the TATA-box Binding Protein

  • Arce, Debora Pamela;Tonon, Claudia;Zanetti, Maria Eugenia;Godoy, Andrea Veronica;Hirose, Susumu;Casalongue, Claudia Anahi
    • BMB Reports
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    • v.39 no.4
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    • pp.355-360
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    • 2006

  • To gain a better understanding on the function of the potato Solanum tuberosum Multiprotein Bridging Factor 1 protein (StMBF1) its interaction with the TATA box binding protein (TBP) was demonstrated. In addition we reported that StMBF1 rescues the yeast mbf1 mutant phenotype, indicating its role as a plant co-activator. These data reinforce the hypothesis that MBF1 function is also conserved among non closely related plant species. In addition, measurement of StMBF1 protein level by Western blot using anti-StMBF1 antibodies indicated that the protein level increased upon $H_2O_2$ and heat shock treatments. However, the potato $\beta$-1,3-glucanase protein level was not changed under the same experimental conditions. These data indicate that StMBF1 participates in the cell stress response against oxidative stress allowing us to suggest that MBF1 genes from different plant groups may share similar functions.

  • https://doi.org/10.5483/BMBRep.2006.39.4.355 인용 PDF

Evaluation of Foliar Uptake of Eight Fungicides Using a New Measuring Tool, Congo Red Method

  • Yu, Ju-Hyun;Lim, He-Kyoung;Cho, Kwang-Yun;Kim, Jeong-Han
    • Journal of Applied Biological Chemistry
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    • v.44 no.1
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    • pp.27-31
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    • 2001

  • Using a new evaluation method, Congo red method, the foliar uptakes of eight fungicides were measured. Among the fungicides tested, fenarimol, myclobutanil, edifenphos, isoprothiolane, and tricyclazole were found to be penetrable (11.0~42.8%), and were continuously absorbed into the leaf regardless of their formulation types. Procymidone, dimethomorpb, and ethaboxan were seldomly absorbed into plant leaf. The uptakes of dimethomorph and ethaboxam were facilitated (18.1~22.7%) in the presence of Koremul-OE-20, as an activator, showing plateau phase at 12h after application. Foliar uptake of fenarimol and myclobutanil into cucumber plant by spray application was larger than that by dropping application, while observed opposite in the cases of dimethomorph and ethaboxam containing activator. Congo red method in conjunction with spraying application used in the present study produced the clear results on foliar uptake of fungicides and therefore proved to be better than the conventional method in which the dropping application of pesticide has been used.

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