• Journal of the Korean Institute of Landscape Architecture
• /
• v.45 no.5
• /
• pp.42-59
• /
• 2017

To investigate rooting and community forming ability, sod strips of Gramineae such as Miscanthus sacchariflorus (Maxim.) Benth., Miscanthus sinensis var. purpurascens(Andersson) Rendle, Imperata cylindrica var. koenigii(Retz.) Pilg., and Arundinella hirta(Thunb.) Tanaka were planted horizontally at a width of 10~15cm in October 2010 on the bank slope of Kwangju Stream in Korea. Seeds of each species were sown in April 2010 in plastic seedbeds(60cm length ${\times}$ 30cm width ${\times}$ 5cm depth) filled with 4cm of garden soil and grew until October 2010 to form rectangular sod, which then was cut into multiple strips(20cm length ${\times}$ 6cm width ${\times}$ 4cm root depth). Weeds growing on the experiment sites were removed twice a year until those four species had formed a community by 2013. Weeds were not eradicated during 2014 and 2015 to observe whether the community formation can be maintained without weed removal. t-tests on stem numbers and heights in May, July, and September were conducted between 2013 and 2014, and between 2014 and 2015. Stem numbers and heights of the four species in 2014 were significantly decreased(p<0.001) compared with those in 2013 due to weed damage to their growth in 2014. Stem numbers and heights of Miscanthus sacchariflorus(Maxim.) Benth., Miscanthus sinensis var. purpurascens(Andersson) Rendle, Imperata cylindrica var. koenigii(Retz.) Pilg. in 2015 were similar to those in 2014(p>0.05). Stem numbers and heights of Arundinella hirta(Thunb.) Tanaka in 2015, however, were significantly reduced compared with those in 2014(p<0.001) due to weed impact on its growth. After three years of weed removal, a community of each species was formed. Miscanthus sacchariflorus(Maxim.) Benth., Miscanthus sinensis var. purpurascens(Andersson) Rendle, Imperata cylindrica var. koenigii(Retz.) Pilg. were able to establish communities without any further weed removal. Arundinella hirta(Thunb.) Tanaka, however, was not able to maintain its community due to considerable damage to its growth caused by weeds even after three years of weed removal. Miscanthus sacchariflorus(Maxim.) Benth., Miscanthus sinensis var. purpurascens(Andersson) Rendle, Imperata cylindrica var. koenigii(Retz.) Pilg. are more suitable to sod strip plantings on stream bank slopes in terms of the maintenance of community formation without weed eradication. t-test on stem numbers and heights each September during the five year experiment period between sod strip planting and potted plants of the four species were conducted. Stem numbers of strip sod plantings were significantly higher(p<0.001) than those of potted one. Heights of strip sod plantings, however, were significantly lower(p<0.05) than those of the potted samples. Therefore, strip sod planting is more advantageous regarding bank slope erosion protection due to the higher number of stems.

• Korean Journal of Microbiology
• /
• v.50 no.3
• /
• pp.201-209
• /
• 2014

This study was carried out in order to develop a biological control of anthracnose of red pepper caused by fungal pathogens. In particular, this study focuses on the Colletotrichum species, which includes important fungal pathogens causing a great deal of damage to red pepper. Antagonistic bacteria were isolated from the soil of pepper fields, which were then tested for biocontrol activity against the Colletotrichum gloeosporioides anthracnose pathogen of pepper. Based on the 16S rRNA sequence analysis, the isolated bacterial strain CS-52 was identical to Bacillus sp. The culture broth of Bacillus sp. CS-52 had antifungal activity toward the hyphae and spores of C. gloeosporioides. Moreover, the substances with antifungal activity were optimized when Bacillus sp. CS-52 was grown aerobically in a medium composed of 0.5% glucose, 0.7% $K_2HPO_4$, 0.2% $KH_2PO_4$, 0.3% $NH_4NO_3$, 0.01% $MnSO_4{\cdot}7H_2O$, and 0.15% yeast extract at $30^{\circ}C$. The inhibition of spore formation resulting from cellulase, siderophores, and indole-3-acetic acid (IAA), were produced at 24 h, 48 h, and 72 h, respectively. Bacillus sp. CS-52 also exhibited its potent fungicidal activity against anthracnose in an in vivo test, at a level of 70% when compared to chemical fungicides. These results identified substances with antifungal activity produced by Bacillus sp. CS-52 for the biological control of major plant pathogens in red pepper. Further studies will investigate the synergistic effect promoting better growth and antifungal activity by the formulation of substances with antifungal activity.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.60 no.1
• /
• pp.63-69
• /
• 2015

This study was conducted to measure the effect of PE film mulching and irrigation methods on the growth, yield and antioxidant activity of potatoes tubers, in order to examine the possibility of cultivating potatoes in winter season in vinyl greenhouse on the reclaimed tidal land with weak ground inside the sea wall currently completed. The test was conducted on the sandy loam soil (Munpo series), and its salt concentration was 0.42% at the time of planting. The emergence speed per kind of PE film mulching was in the order of black > coloration > transparent > green color, with the black color showing the fastest speed. The temperature change during a day per kind of PE film mulching was in the order of transparent > coloration > green > black color. As for the salt concentration in the soil for each different way of water management, the salt concentration in the treatment of drip irrigation with 1 week interval was lower than that drip irrigation with 2 weeks interval. As for the growth of above-aerial part, plant length was higher, number of tiller and leaves were more and dry weight of above-aerial part was larger in the treatment of drip irrigaton with 1 week interval than drip irrigation with 2 weeks interval. As for the yield of potatoes depending on each way of water management, the yield in the treatment of drip irrigation with 1 week interval was more than drip irrigation with 2 weeks interval. The yield for each different kind of PE film mulching in the weekly drip-irrigation management section was in the order of transparent ${\geq}$ black ${\geq}$ coloration ${\geq}$ green color. In both of total phenol content and DPPH free radical activity experiments, the content and activity were higher with pear color vinyl treatment.

• Journal of the Korea Organic Resources Recycling Association
• /
• v.13 no.4
• /
• pp.89-99
• /
• 2005

The golf courses more than about 200 are operating in Korea. From the golf courses, a great amount of turfgrass clippings tend to increase, steadily. Materials used in the experiment were Creeping Bentgrass(CB), Kentucky Bluegrass(KB), Korean Lawngrass(KL), rice bran and composted chicken drop. Treatments are CB, KB, and KL. The temperature during the composting of all treatments increased rapidly and reached at the highest temperature($57.9^{\circ}C$, $67.8^{\circ}C$, $74.3^{\circ}C$) within 20 days, and then stabilized to the range of $35.2{\sim}41.6^{\circ}C$ at the 30th day. The pH values of all treatments decreased on the first day. However, they were increased rapidly after three days and decreased again on 10~20 days. The pH values of all treatments at the final day were stabilized to the low alkali levels. The contents of total carbon during the period of composting tend to decrease and total nitrogen was increased for factor of reduction of volume. CEC value of all treatments during the period of composting tends to increase. The round paper chromatogram of extracted solution of KL sample was the sharpest and clearest among all treatments. The G.I. values of CB, KB, and KL in 30th day of composting were about 95.1, 77.7, and 98.7 in germination test using chinese cabbage, respectively. Conclusively, all turfgrass clippings used in this experiment were composted well, suitable as composting products standardized by KSC. The maturity of the final compost samples is best in KL, followed by CB and KB treatments. The turfgrass compost can contribute to the plant cultivation for environment-friendly farm, and the results of this study can become the basic data of turfgrass clippings compost. Further research on the mixing ratio of each material is required to produce compost of good quality.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
• /
• 2000.11a
• /
• pp.44-63
• /
• 2000

Situated close to Heathrow Airport, and adjacent to the M4 and M25 Motorways, the site at Axis Park is considered a prime location for business in the UK. In consequnce two of the UK's major property development companies, MEPC and Redrew Homes sought the expertise of Intergeo to remediate the contaminated former industrial site prior to its development. Industrial use of the twenty-six hectare site, started in 1936, when Hawker Aircraft commence aircraft manufacture. In 1963 the Firestone Tyre and Rubber Company purchased part of the site. Ford commenced vehicle production at the site in the mid-1970's and production was continued by Iveco Ford from 1986 to the plant's decommissioning in 1997. Geologically the site is underlain by sand and gravel, deposited in prehistory by the River Thames, with London Clay at around 6m depth. The level of groundwater fluctuates seasonally at around 2.5m depth, moving slowly southwest towards local streams and watercourses. A phased investigation of the site was undertaken, which culminated in the extensive site investigation undertaken by Intergeo in 1998. In total 50 boreholes, 90 probeholes and 60 trial pits were used to investigate the site and around 4000 solid and 1300 liquid samples were tested in the laboratory for chemical substances. The investigations identified total petroleum hydrocarbons in the soil up to 25, 000mg/kg. Diesel oil, with some lubricating oil were the main components. Volatile organic compounds were identified in the groundwater in excess of 10mg/l. Specific substances included trichloromethane, trichloromethane and tetrachloroethene. Both the oil and volatile compounds were widely spread across the site, The specific substances identified could be traced back to industrial processes used at one or other dates in the sites history Slightly elevated levels of toxic metals and polycyclic aromatic hydrocarbons were also identified locally. Prior to remediation of the site and throughout its progress, extensive liaison with the regulatory authorities and the client's professional representatives was required. In addition to meetings, numerous technical documents detailing methods and health and safety issues were required in order to comply with UK environmental and safety legislation. After initially considering a range of options to undertake remediation, the following three main techniques were selected: ex-situ bioremediation of hydrocarbon contaminated soils, skimming of free floating hydrocarbon product from the water surface at wells and excavations and air stripping of volatile organic compounds from groundwater recovered from wells. The achievements were as follows: 1) 350, 000m3 of soil was excavated and 112, 000m3 of sand and gravel was processed to remove gravel and cobble sized particles; 2) 53, 000m3 of hydrocarbon contaminated soil was bioremediated in windrows ; 3) 7000m3 of groundwater was processed by skimming to remove free floating Product; 4) 196, 000m3 of groundwater was Processed by air stripping to remove volatile organic compounds. Only 1000m3 of soil left the site for disposal in licensed waste facilities Given the costs of disposal in the UK, the selected methods represented a considerable cost saving to the Clients. All other soil was engineered back into the ground to a precise geotechnical specification. The following objective levels were achieved across the site 1) By a Risk Based Corrective Action (RBCA) methodology it was demonstrated that soil with less that 1000mg/kg total petroleum hydrocarbons did not pose a hazard to health or water resources and therefore, could remain insitu; 2) Soils destined for the residential areas of the site were remediated to 250mg/kg total petroleum hydrocarbons; in the industrial areas 500mg/kg was proven acceptable. 3) Hydrocarbons in groundwater were remediated to below the Dutch Intervegtion Level of 0.6mg/1; 4) Volatile organic compounds/BTEX group substances were reduced to below the Dutch Intervention Levels; 5) Polycyclic aromatic hydrocarbons and metals were below Inter-departmental Committee for the Redevelopment of Contaminated Land guideline levels for intended enduse. In order to verify the qualify of the work 1500 chemical test results were submitted for the purpose of validation. Quality assurance checks were undertaken by independent consultants and at an independent laboratory selected by Intergeo. Long term monitoring of water quality was undertaken for a period of one year after remediation work had been completed. Both the regulatory authorities and Clients representatives endorsed the quality of remediation now completed at the site. Subsequent to completion of the remediation work Redrew Homes constructed a prestige housing development. The properties at "Belvedere Place" retailed at premium prices. On the MEPC site the Post Office, amongst others, has located a major sorting office for the London area. Exceptionally high standards of remediation, control and documentation were a requirement for the work undertaken here.aken here.

• Journal of Korean Society on Water Environment
• /
• v.18 no.3
• /
• pp.303-312
• /
• 2002

Polly-pigs technique was developed to remove internal rusts and scales from service pipes in the building by using Polly-pigs that were used as various applications in conventional plant pipelines. Results of cleaning experiments on 15mm GSP(Galvanic Steel Pipe) showed that hydraulic cross sectional area was more increased from 3.5% to 15.4% for straight part or elbow parts of several test pieces. Results of optical analysis also showed that rust and scale removed mostly by KDP series(only consisted of Polyurethane) were outside red colored scale ($Fe_2O_3{\cdot}H_2O$), and there was a limitation to the removal of black colored scale($Fe_2O{\cdot}nH_2O$) that was below red colored scale. But it was evaluated that KDPS series coated fine sand with KDP series could remove not only red colored scale but also black colored scale and more increase hydraulic cross sectional areas of 15mm GSP old service pipes from 13.0% to 17.9%. After KDPS series cleaning, hydraulic cross sectional areas of them were recovered from 95.9% to 99.5%. Turning force of Polly-pigs was largely improved by the effect of Helical guide vane(Cleaning v/v) and Rotating wing(Pigs), and the number of pig rotating also more increased sixteen times compared with conventional cleaning system without Helical guide vane and Rotating wing. After KDPC series cleaning of 100mm GSP old service pipes that hydraulic cross sectional areas were 90%, hydraulic cross sectional areas were almost recovered perfectly like new service pipes. Additionally pressure necessary to run Polly-pigs m 100mm GSP was lower and cleaning efficiency also was higher than 15mm GSP cleaning. Therefore it was thought that as the diameter of pipe was more increased, pressure necessary to clean service pipes was more decreased and cleaning efficiency was more increased in Polly-pigs cleaning.

• Korean Journal of Weed Science
• /
• v.31 no.3
• /
• pp.294-307
• /
• 2011

This experiment carried out to confirm characteristics of macrogranule (GG) for herbicidal efficacy by using remote-controlled aerial application (RCAA) to control annual and perennial weeds in rice paddy field, application methods, and application times of formulation types. Particle of 500 g GG having average diameter of 2.5~3 mm was over 85 percent, the bulk density of the particle was $0.2\sim0.4g\;mL^{-1}$ and the particle was water floating granule. Active ingredients and external form of halosulfuronmethyl+mefenacet GG remained stable under condition of storage stability test. The disintegration time of the GG was faster as the water temperature was increased. But disintegration time was not affected by pH on the water conditions. By using hand, spoon and power applicator, drift distances of GG were 4~5m, 7 m and 10~12 m, respectively. GG showed good herbicidal efficacy and plant safety in all of the application methods such as, using hand, spoon, power applicator and RCAA. Application times of GG, GR, SC and TB by hand in $4,000m^2$ were 38 min. 4 sec, 42 min. 20 sec, 38 min. 10 sec and 21 min. 4 sec, respectively, but application time of GG by using RCAA was 1 min 32 sec. According to appearance and characteristics of formulation types, suspension concentrate (SC) and GG were possible formulation types for RCAA.

• Horticultural Science & Technology
• /
• v.31 no.6
• /
• pp.772-781
• /
• 2013

The objective of this study was to develop simple sequence repeat (SSR) markers from expressed sequence tags (EST) of lettuce (Lactuca sativa) and identify 9 germplasms from 3 wild species of lettuce and 61 commercial cultivars using the developed EST-SSR markers. A total of 81,330 lettuce ESTs from NCBI databases were used to search for SSR and 4,229 SSR loci were identified. The highest proportion (59.12%, 2500) was represented by trinucleotide, followed by dinucleotide (29.70%, 1256) and hexanucleotide (6.62%, 280) among SSR repeat motifs. Totally 474 EST-SSR primers were developed from EST and a random set of 267 primers was used to assess the genetic diversity among 9 germplasms and 61 cultivars. Out of 267 primers, 47 EST-SSR markers showed polymorphism between 7 cultivars. Twenty-six EST-SSR markers among 47 EST-SSR markers showed high polymorphism, reproducibility, and band clearance. The relationship between 26 markers genotypes and 70 accessions was analyzed. Totally 127 polymorphic amplified fragments were obtained by 26 EST-SSR markers and two to nine SSR alleles were detected for each locus with an average of 4.88 alleles per locus. Average polymorphism information content was 0.542, ranging from 0.269 to 0.768. Genetic distance of clusters ranged from 0.05 to 0.94 between 70 accessions and dendrogram at a similarity of 0.34 gave 7 main clusters. Analysis of genetic diversity revealed by these 26 EST-SSR markers showed that the 9 germplasms and 61 commercial cultivars were discriminated by marker genotypes. These newly developed EST-SSR markers will be useful for cultivar identification and distinctness, uniformity and stability test of lettuce.

• Korean Journal of Weed Science
• /
• v.15 no.1
• /
• pp.85-98
• /
• 1995

Six malformin B's produced by Aspergillus niger van Tiegh. were separated by HPLC. Their structures determined by the methods of amino acid analyses, mass spectrometry, and two-dimensional NMR were revealed as cyclic pentapeptides structurally related to malformin $A_1$. Both the NMR and MS/MS data suggest that the respective structures of separated malformin B's were as follows; cyclo-D-Cys-D-Cys-L-Val-D-Leu-L-allo-Ile for $B_{1a}$, cyclo-D-Cys-D-Cys-L-Val-D-Leu-L-Leu for $B_{1b}$, cyclo-D-Cys-D-Cys-L-Val-D-Val-L-Leu for $B_2$, cyclo-D-Cys-D-Cys-L-Val-D-Ile-L-Leu for $B_3$, cyclo-D-Cys-D-Cys-L-Val-D-Ile-L-Ile for $B_4$, and cyclo-D-Cys-D-Cys-L-Val-D-Val-L-Ile for $B_5$. Among the malformin B's, the structure of $B_{1b}$ was the same as that of malformin $A_3$ or C. All the malformin B's showed physiological activities in the two assay systems using corn(Zea mays L.) roots and mung bean(Phaseolus aureus Roxb.) hypercotyl segments. The malformin B's with molecular weight 529 were more effective for inducing corn root curvature than those with molecular weight 515. The difference in molecular weight of malformin B's, i.e., the retention time on HPLC, results in the polarity change of the whole malformin molecule which affects the revealation of the malformin activities. In addition, the disulfide form of the malformin B's gives the rigidity of the molecule, whereas the combination of the fourth and the fifth amino acid residues provides the optimal three-dimensional configuration to the malformin receptor of plants. Presumably, these two factors are appeared to be essential for the greatest physiological activity of malformin B's. malformin $B_{1a}$ caused the corn root curvature by 90% at a concentration of $0.25{\mu}M$. However, such differential activities with molecular weight of 529 or 515 of malformin B's were not found in the mung bean hypercotyl segment test. Maximum stimulation of mung bean hypercotyl growth was observed at $0.1{\mu}M$ concentration of malformin B's. The growth of the segments treated with $B_5$ was 154% greater than that of the control.

• Journal of the Mineralogical Society of Korea
• /
• v.16 no.4
• /
• pp.283-293
• /
• 2003

Domestic water treatment plants operate the rapid and slow filtering system using the filtering sands. Most of them are composed of beach sands, which have less sorption capacity of heavy metals as well as organic contaminants. Therefore, the development of fortified functional filtering materials with high removal capacity of organic and inorganic contaminants is needed to prevent the unexpected load of contaminated source water. This study aims to test the hydrochemical change and the removing capacity of heavy metals such as Cd, Cu, and Pb on the Jumunjin sand, feldspathic sand(weathering product of Jecheon granite), feldspathic mixing sand I(feldspathic sand mixed with 10 wt% zeolite), and feldspathic mixing sand II (feldspathic sand mixed with 20 wt% zeolite). Feldspathic mixing sand I and II showed the eruption of higher amounts of cations and anions compared with the Jumunjin sand and feldspathic sand. They also showed higher eruption of Si, Ca, $SO_4$ ions than that of Al, $NO_3$, Fe, K, Mg, and P. Feldspathic mixing sand II caused higher eruption of some cations of Na, Ca, Al than feldspathic mixing sud I, which is the result controlled by the dissolution of zeolite. Jumunjin sand and feldspathic sand showed very weak sorption of Cd, Cu and Pb. In contrast to this, feldspathic mixing sand I and II showed the high sorption and removal capacity of the increasing order of Cd, Cu and Pb. Feldspathic mixing sand II including 20% zeolite showed a fortified removal capacity of some heavy metals. Therefore, feldspathic mixing sand mixed with some contents of zeolite could be used as the fortified filtering materials for the water filtering and purification in the domestic water treatment plants.