• Journal of Species Research
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• 제9권4호
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• pp.413-426
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• 2020

The Euphorbiaceae family features some of the most economically important plants that are sources of foods, oils, waxes, and medicines. The accurate identification of Euphorbiaceae species is critical in sustainable utilization of plant resources. We examined 234 sequences of nrDNA ITS, cpDNA rbcL and matK loci from 20 species in Euphorbiaceae in Korea and three outgroup taxa to develop efficient DNA barcodes. The three barcode loci were successfully amplified and sequenced for all Euphorbiaceae species. nrDNA ITS locus showed the highest mean interspecific K2P distance (0.3034), followed by cpDNA matK (0.0830), and rbcL (0.0352) locus. The degree of species resolution for individual barcode loci ranged from 75% (rbcL and matK) to 80% (ITS). The degree of species resolution was not enhanced with the different combinations of three barcode loci. The combined data set of the three loci(ITS+rbcL+matK) provided 80% of species resolution. These results confirm that ITS locus, as a single barcode, is the best option for barcoding of the Euphorbiaceae in Korea.

• The Plant Pathology Journal
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• 제21권4호
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• pp.310-316
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• 2005

The objectives of this study were to identify the aecial host ranges of four Korean Gymnosporangium species, G. asiaticum, G. cornutum, Gjaponicum and Gyamadae, and to verify the morphological characteristics of telia as diagnostic keys to the species. Thirteen Korean Rosaceous woody species were artificially inoculated with teliospores obtained from Juniperus species. There was high specificity between telial and aecial hosts and the fungal species, providing the first experimental proof on host alternation of these rust fungi in Korea. Telia on the witches' broom and on the small galls were identified as new telial characteristics in G asiaticum and in G. yamadae, respectively. Aecial hosts of G. asiaticum and G. yamadae showed varying responses in their susceptibility and in the days required for formation and duration of spermogonia and aecia after inoculation. Four telial host species in Juniperus were confirmed for the first time in Korea, which include J. chinensis var. kaizuka, J. chinensis var. horizontalis and J. chinensis var. globosa for G. asiaticum; and J. chinensis var. kaizuka for G. yamadae.

• The Plant Pathology Journal
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• 제15권4호
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• pp.228-235
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• 1999

Genetic diversity of ninety-five Korean isolates of Phytophthora was investigated on the basis of PCR-RFLP of ribosomal DNA. The isolates were previously identified as following fifteen species by mycological and cultural characteristics; P. boehmeriae, P. cactorum, P. cambivora, P. capsici, P. cinnamoni, P. citricola, P. citrophthora, P. cryptogea, P. drechsleri, P. erythroseptica, P. infestans, P. megasperma, P. nicotianae, P. palmivora and P. sojae. The regions of small subunit (SSU) and internal transcribed spacer (ITS) of rDNA were amplified with primer pair, NS1 and ITS4, by polymerase chain reaction (PCR) and digested with nine restriction enzymes. P. boehmeriae, P. cactorum, P. cambivora, P. capsici, P. cinnamomi, P. citricola, P. citrphthora, P. infestans, P. nicotianae and P. palmivora showed specific band patterns for each species. However, P. sojae and P. erythroseptica presented identical band patterns and P. cryptogea, P. drechsleri and P. megasperma were divided into six groups, which were not compatible with delineation of the species. A group originated from cucurbits showed distinct band patterns from other groups, but the other five groups were closely related within 96.0% similarity, forming one complex group. Consequently, Korean isolates of Phytophthora were divided into thirteen genetic groups and each group was readily differentiated by comparing digestion patterns of AvaII, HaeIII, MboI, HhaI and MspI. Therefore, PCR-RFLP of rDNA using the five enzymes can be used to differentiate or identify the Phytophthora species reported in Korea so far.

• 식물분류학회지
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• 제44권1호
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• pp.9-12
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• 2014

Talipariti simile (Blume) Fryxell (아욱과)을 베트남 미기록 종으로 처음으로 보고한다. 본 종은 줄기에 털이 없는 유사종인 T. macrophyllum (Roxb. ex Hornem.) Fryxell 와는 달리 줄기에 자색을 띠는 작은 털이 있으며, 탁엽은 피침상 타원형으로 크기가 작으며, 삭과는 난형이며 끝이 뾰족하지 않거나 부리모양이 아니다. 종 동정을 위한 분류학적 기재, 식물도해, 원색사진을 첨부하였다. 또한 유사 종들과의 비교를 위한 검색형질을 제시하였다.

• 식물분류학회지
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• 제46권1호
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• pp.55-59
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• 2016

Justicia kampotiana Benoist (쥐꼬리망초과)를 베트남 미기록종으로 보고한다. 본 종은 유사종인 J. ingrata Benoist와 달리 외화관에 털이있으며, 화통은 판연 보다 약간 길고 화병보다는 아주 짧다. 종 동정을 위한 기재, 그림도해 및 컬러사진과 함께 본 종과 유사종의 비교를 위한 검색형질들을 제시하였다.

• Animal Systematics, Evolution and Diversity
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• 제36권1호
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• pp.41-45
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• 2020

The genus Philanthus Fabricius, 1790 in Korea are reviewed, and two species, Philanthus triangulum and P. coronata, are treated. The former is new to Korea. This species is easily separated from congeners by dense punctures in propodral enclosure and tri-forked marking in lower face. DNA barcoding test is supportive in our identification as well as conspecitficity of Korean materials showing variation in size and coloration. The observed data on flower associations of this species specified with domestic localites and date are separately provided. The current status of Philanthus coronatus that has been the only representative of the genus but forgotten for a lengthy time in Korea is discussed.

• 한국응용곤충학회지
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• 제51권2호
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• pp.163-170
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• 2012

Aleurolobus marlatti (Quaintance), Massilieurodes formosensis (Takahashi) 및 Pealius rhododendri Takahashi는 한국에서 처음으로 기록된다. 이들 종에 대한 요약된 정보 및 사진자료와 함께 한국에 발생하는 가루이과 종 검색표를 업데이트하였다.

• Journal of Plant Biology
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• 제38권3호
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• pp.297-303
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• 1995

Four species of Symphyocladia growing along the coast of Korea were investigated in taxonomic point of view. Characters available for identification of the species were; difference in gross morphology, degree of cortication in vegetative axis, presence or absence of vegetative trichoblasts, and developmental pattern of stichidia. Vegetative structure and reproductive organs in developmental anatomy were similar among species. Branching pattern and occurrence of vegetative trichoblasts adopted for systematic criteria between Pterosiphonieae and Polysiphonieae, and between Symphyocladia and Pterosiphonia were no more confidential. The degree of congenital fusion of laterals with the parent axes was considered as only main character to distinguish Symphyocladia from Pterosiphonia.

• 한국식물병리학회지
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• 제11권1호
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• pp.30-38
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• 1995

Anthracnose fungi associated with soybean (Glycine max), small red bean (Vigna angularis), and green bean (Phaseolus radiatus var. aurea) were compared with respect to cultural characteristics, morphological aspects, and pathogenicity. From the legumes, four different species of Colletotrichum were identified based on their morphological characters : C. truncatum, C. destructivum, C. trifolii and C. gloeosporioides. Colonies of Colletotrichum spp.on culture media varied in colour and growth habits depending upon the media and isolates. In general, formation of conidia, setae, and perithecia on water agar leaf medium was better than that on potato dextrose agar medium under near ultraviolet light. In cross inoculation tests, isolates of C. truncatum, c. destructivum, C. trifolii and C. gloeosporioides could infect soybean, small red bean, and green bean at different growth stages. There were some differences in pathogenicity or susceptibility depending on crops tested. C. truncatum was the most virulent and major pathogenic species among the four species.

• 한국응용곤충학회지
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• 제24권4호
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• pp.191-194
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• 1986

현사시나무(Populus $alba{\times}glandulosa$)의 줄기 식입해충중(害蟲中) 우점종은 포푸라하늘소(Compsidia populnea L.)였으며 황철나무하늘소(Compsidia balsamifera M.)는 확인되지 않았다. 본(本) 조사(調査) 통하여 상기 두 종(種)의 형태(形態), 분포(分布), 생활사등(生活史等)이 비교 검토됨으로서 포푸라하늘소의 정확한 분류동정(分類同定)이 가능(可能)하여졌다.