• Journal of Plant Biotechnology
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• v.44 no.3
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• pp.335-342
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• 2017

Purple passion fruit (Passiflora edulis Sims) is one of the introduced tropical plants, an increasing interest has arisen due to its distinctive taste and attractive flavor. It is expected that passion fruit production and planted area will increase gradually in the years ahead because of high profitability and consumer's demands of healthful ingredients. So we tried to investigate the effect of plant growth regulators and antioxidants on in vitro plant regeneration and callus induction from leaf explants of passion fruit for an establishment of optimal mass propagation system. Young leaf explants of purple passion fruit were cultured in Murashige and Skoog (MS) medium containing different growth regulators and antioxidant additives to induce the shoot organogenesis. After 8 weeks, the highest embryogenic callus formation rate was obtained in MS medium supplemented with $1mg{\cdot}L^{-1}$ 6-benzylaminopurine (BAP) and $2mg{\cdot}L^{-1}$ 2,4-dichlorophenoxyacetic acid (2,4-D), furthermore, the shoot development via organogenesis was also observed. Silver nitrate ($AgNO_3$), which was added into the medium to minimize the adverse effects of leached phenolics, was effective for reduction of medium browning and sudden explant death. In the medium supplemented with $1mg{\cdot}L^{-1}$ BAP and $1mg{\cdot}L^{-1}$ gibberellic acid ($GA_3$), shoots were most vigorously regenerated and elongated. Most shoots rooted successfully in half strength medium with $1mg{\cdot}L^{-1}$ indol-3 acetic acid (IAA), and more than 90% of plantlets survived after 4-month acclimatization period.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2021.04a
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• pp.23-23
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• 2021

Apple (Malus×domestica Borkh.; Rosaceae) is an important fruit crop grown mainly in temperate regions of the world. Tissue culture in vitro is a biotechnological technique that has been used to genetically improve cultivars (scions) and rootstocks. This could be important in the production of genetically uniform scions and rootstocks for commercial apple production. In nurseries, apple plants are produced by grafting scions onto rootstocks. The Cornell-Geneva (Geneva® series) breeding program has bred several dwarf rootstocks that are resistant to diseases and pests and are also cold hardy. This study was conducted to determine the optimal medium strength to improve sprouting shoot rate of apical meristem of the apple rootstocks of fire blight resistance. The apple rootstocks apical meristem at size (0.2 mm to 0.3 mm) with axillary buds were cultured on the MS(Murashige & Skoog) medium supplemented with plant growth regulators. The sprouting ratio and growth characteristics was evaluated after eight weeks in vitro culture. The highest rate of bud differentiation and shoot formation were 23.8% and 55.6%, respectively. After 6 weeks, shoots were regenerated from apical meristem, and their growth characteristics was significantly varied on the respective basal medium with different plant growth regulators. Our studies showed that the apple rootstocks the apple rootstocks of fire blight resistance plantlets could be successfully produced from apical meristem differentiated out of young twigs via organogenic regeneration.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.34 no.s01
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• pp.4-15
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• 1989

The objective of this paper is to compare and summarize the procedure and effectiveness of some bioassay systems and to point out ways to obtain reliable results from each bioassay. Detailed C:escriptions were given for those widely-adapted bioassay methods, such as mungbean rooting (auxin), Avena first internode straight growth (auxin), dwarf rice growth (gibberellin), dwarf pea epicotyl elongation (gibberellin), radish cotyledon expansion test (cytokinin), and tobacco stem pith callus growth (cytokinin), and the effects of various plant growth regulators including some recently introduced growth retardants (Paclobutrazol, Uniconazol, etc.) were also summarized.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2000.05a
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• pp.69-71
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• 2000

• Proceedings of the Plant Resources Society of Korea Conference
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• 2002.04a
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• pp.51-51
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• 2002

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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• v.2 no.2
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• pp.159-168
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• 1998

Effect of plant growth regulators and end-product on the enzyme activities in cotyledons of Vigna angularis during germination was investigated by measuring the changes of $\alpha-amylase$ activities in attached and detached cotyledons applied growth regulators and sugars. The higher levels of $\alpha-amylase$ in detached cotyledons than those in cotyledons attached to the embryonic axis were due to both faster synthesis and slower degradation of the enzyme in the detached cotyledons than in the attached cotyledons. Levels of $\alpha-amylase$ activity were reduced by high concentrations of glucose and sucrose, and it is suggested that this effect was caused mostly by osmotic stress and partly by end-product repression. In detached cotyledons exogenously supplied $GA_3,$ IAA, kinetin, or their combinations has a small promotive effect on the developmental patterns of $\alpha-amylase$ activity ABA and uniconazole both prevented the synthesis of $\alpha-amylase$. Glucose inhibition of enzyme activity was partly reversed by the application of $GA_3,$ and CAMP. $GA_3,$ and cAMP seemed to act through a similar mechanism. The addition of inhibitors of protein and RNA synthesis largely prevented the increase of enzyme activity in the presence or absence of exogenous $GA_3,$. The pretreatment experiments with canavanine indicated that the earlier the time of addition was, the lower the amylase activity was.

• Journal of Ginseng Research
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• v.10 no.2
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• pp.159-166
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• 1986

Effects of plant growth regulators on the germination of ginseng (Panax ginseng C.A. Meyer) seeds were investigated. Ginseng seeds germinated more vigorously in the treatments of kinetin and BA, and the promoting effect of kinetic on the germination and the growth of rootlet enhanced in low temperature ($10^{\circ}C$). However, GA did not promote the germination of dehiscent seed. The optimum temperature for germination of dehiscent seed was $10^{\circ}C$ and the range of effective concentration of kinetin for germination was 50 to 100 ppm.

• Journal of Life Science
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• v.9 no.5
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• pp.564-569
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• 1999

This study was conducted to determine the concentrations of plant growth regulators required for regeneration and the concentrations of antibiotics for the selection of transformed regenerants from lettuce, musk melon and tomato. The optimal concentrations of plant growth regulators for shoot formation were NAA 0.1 mg/$\ell$ +BA 0.1 mg/$\ell$ for lettuce, NAA 0.01 mg/$\ell$ +BA 2.0 mg/$\ell$ for melon and NAA 0.1 mg/$\ell$ +BA 0.5 mg/$\ell$for musk melon. Shoot induction from tomato, lettuce and melon was completely inhibited by 30 mg/$\ell$ or higher concentrations of kanamycin. Shoot formation from mu나 melon was not affected by kanamycin up to 40 mg/$\ell$, but was reduced in the presence of 50 mg/$\ell$ and completely inhibited by 100 mg/$\ell$. Shoot formation of all four crops was completely inhibited by higromycin at 10 mg/$\ell$. Both carbenicillin and cefatoxinme did not show any negative effects on shoot formation.

• Journal of Bio-Environment Control
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• v.6 no.4
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• pp.277-283
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• 1997

The effect of nutrient concentration and plant growth regulators on rooting of Coleus stem cuttings were investigated. In contrast to sand cutting, the nutrient solution or plant growth regulator treatment increased the root numbers and root length. The concentration of nutrient solution were 1, l/2 and 1/4 strength of the balanced nutrient solution developed by Japanese Horticultural Experimental Station and the highest root growth was obtained from 1/4 strength nutrient concentration. NAA or IBA treatment in culture solution stimulated the rooting and 0.01mg/l of NAA or IBA was effective in root development. Furthermore, days for rooting were shortened and root growth were promoted by mixing nutrient solutions with plant growth regulators and by using cutting with leaves. The rooting of Coleus was observed under microscope and the adventitious root formation initiated at 3 days after cutting with nutrient and auxin mixture.

• Korean Journal of Medicinal Crop Science
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• v.23 no.1
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• pp.13-19
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• 2015

This study was conducted to improve the postharvest storage techniques of managing and storing seeds, to test qualities and viabilities of the seeds and to examine the germination rate and the protein expression of Achyranthes japonica Nakai. The seeds collected from different areas of Je-Cheon and Gwang-Ju were stored with different temperatures and durations. Two plant growth regulators and two seed priming were treated to investigate their effect on the germination rates and the days required for germination. The weight of one hundred seed collected in Gwang-Ju was heavier than those in Je-Cheon. Seed length collected in Gwang-Ju was also longer about 5.12 mm than those in Je-Cheon about 4.90 mm and seed width was longer in Gwang-Ju than those in Je-Cheon. The rates of seed germination in two different collection areas were higher about 2.9 to 13.0% in Gwang-Ju compared to those in Je-Cheon. Comparing its rates with the storing temperatures and durations, they were not clearly different in between $4^{\circ}C$ and $25^{\circ}C$ and they also were gradually decreased with getting longer storing durations. The germination rates treated by plant growth regulators were higher with $GA_3$ than those with Kinetin. The highest seed germination rate was appeared at 50 ppm of $GA_3$. Comparing its rates with different seed priming, they were relatively higher with $KNO_3$ than those with PEG6000. In protein expression patterns between before the germinating and after the germinating of seeds, more and clear bands were appeared in the seed after the germination compared to those before the germination of seeds, especially 10 ~ 20 kDa. These results showing more and clear bands were more clearly appeared in Gwang-Ju compared to Je-Cheon. Comparing the protein expression with plant growth regulators and seed primings, $GA_3$ was better expression than those with Kinetin and $KNO_3$ was better than those with PEG6000. More and clear bands were closely related to the germination rates of seeds and more detailed studies would be required.