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Behavior Monitoring System based on WAP for the Elderly and the Disabled (장애인 및 노약자를 위한 WAP 기반 행동 모니터링 시스템)

  • 김택현;이희영
    • Proceedings of the IEEK Conference
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    • 2003.07c
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    • pp.2565-2568
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    • 2003
  • This paper presents a behavioral data monitoring system based on WAP(wireless application protocol) service for the 24-hour continuous health state monitoring of the elderly and the disabled. The developed system transmits a character message to the predefined mobile cell phone through SMS service when an emergency state takes place. Simultaneously, the image captured by a CCD camera is transmitted to the server computer installed WAP service program. Then, the user of the cell phone who received he message can access the server and open the transmitted image. This system can be used for the effective health monitoring of the elderly and disabled.

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Effects of Warning Labels of Consumption Goods on Consumer Behavior (공산품 소비제품의 경고표시가 소비자행동에 미치는 영향)

  • Yang Deok Soon;Song In Sook
    • Journal of the Korean Home Economics Association
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    • v.43 no.4 s.206
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    • pp.141-159
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    • 2005
  • The purpose of this study is to examine the effects of warning labels of consumption goods on consumer behavior and the consumers' evaluation in order to suggest a policy of warning labels and the Product Liability Law. The following items which are somewhat risky but are used in everyday the were selected: cup noodles, one-touch can foods, washing machine, microwave range, dry cell, hydro-oxygen detergent, baby-walker, and block-toys. Data were collected from 345 respondents by internet survey with the following results. (1) The average score of the effects of warning labels on consumer behavior was so low that warning labels are not considered effective, especially in block-toys, dry cell, and baby-walker. (2) Consumers' evaluation scores on the character size, design and sticking place of warning labels were very low. Therefore an effort to make warning labels more effective is needed.

Regulation of Histone Acetylation during First Mitosis in Bovine Clone Embryos

  • Gabbine Wee;Koo, Deog-Bon;Kang, Man-Jong;Moon, Seung-Ju;Lee, Kyung-Kwang;Han, Yong-Mahn
    • Proceedings of the KSAR Conference
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    • 2004.06a
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    • pp.189-189
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    • 2004
  • Histone acetylation as epigenetic marker plays a critical role in gene expression through the interaction of nucleosomes with DNA, modulating the efficiency which RNA-polymerase can interact with promotors to initiate transcription. After fertilization, highly acetylated chromatin takes place and maintain during 1cell stages. The hyperacetylation may lead minor genome activation for survival and cleavage, and then may affect embryonic genome activation and development to balstocyst. (omitted)

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Development of an electrochemi-Iuminescenece device (전기화학형 발광소자 개발)

  • Kwon, Hyuk-Moon;Sung, YouI-Moon;Ji, Jong-Gook;Lee, Myung-Hee
    • Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
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    • 2009.10a
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    • pp.141-144
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    • 2009
  • In this study, used simplest sandwich cells containing $Ru2^+$ liquid electrolytes in order to clarify the role of nanoporous $TiO_2$ electrodes. And, the cell structure is as follow: $F:SnO_2$ glass/ nanoporous $TiO_2$/ tris(2,2'-bipyridy)ruthenium(ll) colplex [$Ru(bpy)_3(PF_6)_2$] in acetonitrile/ $F:SnO_2$ glass. The result, we found that ECL intensities increased rapidly by use of cathodes with nanoporous $TiO_2$ layers. And, porous $TiO_2$ electrodes were confirmed to be efficient for ECL devices as well as solar cell devices. It is thought that the increases in the ECL intensities may be associated with both formation of $Ru^+$ in porous $TiO_2$ electrodes and the process taking place after reduction of $Ru^+$ which occurs in the nanoporous electrodes.

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Effect of Triterpenoidal Glycosides of Dammarane Series and Their Aglycones on Phase Transitions of Dipalmitoylphosphatidylcholine (DPPC의 상전이에 미치는 Dammarane Series의 Triterpenoidal Glycoside와 그 Aglycone의 영향)

  • Kim, Yu.A.;Park, Kyeong-Mee;Park, Hwa-Jin
    • Journal of Ginseng Research
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    • v.20 no.1
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    • pp.23-29
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    • 1996
  • The effect of ginseng glycosides and their aglycones on the thermodynamic characteristics of membranes from dipalmitoylphosphatidylcholine (DPPC) was investigated. Total saponins (TS) from Korean red ginseng, Panax ginseng C.A. Meyer, interacted with the Eel Phase of lipid in the Polar region and did not penetrate the deeper glycerol backbone of lipid molecule. From the all investigated components of TS (aglycons and ginsenosides), only 20-(S)-panaxadiol (PD) had an effect similar to TS. High concentration of TS penetrated in hydrophobic Cl-C8 region. The presence of cholesterol did not influence the interaction of TS with DPPC. An elimination of transition, however, took place at 10~100 $\mu\textrm{g}$/ml of TS. DPPC had a low ability to interact with cholesterol (CHL) as compared with other lecithins except ethanolamine. From our results, only TS and PD, at high concentrations (100 mol%), influenced the phase transition of mixture of DPPC:CHL.

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The Foaming Characteristics of Microcellular Processing with Polypropylene in Semicrystalline States (결정성 수지의 발포특성)

  • Lee, Bo-Hyoung;Cha, Sung-Woon;Yoon, Jae-Dong
    • Proceedings of the KSME Conference
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    • 2003.11a
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    • pp.1828-1833
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    • 2003
  • In a foaming process of microcellular plastics (MCPs) with a batch process, amorphous plastics and crystalline plastics have different characteristics for a foaming temperature. It is known that a foaming of amorphous plastics occurs at the temperature above a glass transition temperature, however, it is discovered that crystalline plastics do not take place above a glass transition temperature without exception, and even though the foaming occurs, it does not in all the range. In this research, to measure foaming temperature of crystalline polymer, a foaming experiment was performed using one of the typical crystalline polymer, polypropylene. To analyze whether the foaming occurs both at amorphous and crystalline fields, SEM was applied

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Ultrastructural Changes in Midgut of CPV infected Tropical Tasar Silkworm, Antheraea mylitta (D) (Lepidoptera : Saturniidae)

  • Barsagade, Deepak Deewaji;Kadwey, Mangala Nimbaji
    • International Journal of Industrial Entomology and Biomaterials
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    • v.21 no.1
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    • pp.117-125
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    • 2010
  • The tropical tasar silkworms, Antheraea mylitta (D) produce famous silk 'Kosa' in central part of India. Due to outdoor rearing it became susceptible to viral infection including cytoplasmic polyhedrosis virus (CPV). The common mode of entry of cytoplasmic polyhedrosis virus is per os and cause gresserie disease to the larvae. Histopathological studies elucidated the insect CPV virus produces infective polyhedral inclusion bodies (PIBs) in the midgut cell cytoplasm of virus infected fifth instar larvae. The PIBs multiply enormously in the cytoplasm without invading the nucleus. Ultrastructural studies confirmed the pathological effects of CPV on in midgut cell cytoplasm. The multiplication of polyhedral inclusion bodies took place into the vacuoles and form virogenic stromata in the cytoplasm of cells. However, the encapsulations of polyhedral inclusion bodies into the polyhedrin protein occurred and polyhedra were released into the lumen. At the late stage of infection, cells showed the regressed cytoplasmic organelles with large vacuoles and elongated mitochondria. Hence, the horizontal transmission of CPV causing the midgut cells disintegration in the tasar silkworm, Antheraea mylitta (D) confirmed during infection.

Ferroptosis-Like Death in Microorganisms: A Novel Programmed Cell Death Following Lipid Peroxidation

  • Min Seok Kwun;Dong Gun Lee
    • Journal of Microbiology and Biotechnology
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    • v.33 no.8
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    • pp.992-997
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    • 2023
  • Ferroptosis is a new kind of programmed cell death of which occurrence in microorganisms is not clearly verified. The elevated level of reactive oxygen species (ROS) influences cellular metabolisms through highly reactive hydroxyl radical formation under the iron-dependent Fenton reaction. Iron contributes to ROS production and acts as a cofactor for lipoxygenase to catalyze poly unsaturated fatty acid (PUFA) oxidation, exerting oxidative damage in cells. While ferroptosis is known to take place only in mammalian cells, recent studies discovered the possible ferroptosis-like death in few specific microorganisms. Capacity of integrating PUFA into intracellular membrane phospholipid has been considered as a key factor in bacterial or fungal ferroptosis-like death. Vibrio species in bacteria and Saccharomyces cerevisiae in fungi exhibited certain characteristics. Therefore, this review focus on introducing the occurrence of ferroptosis-like death in microorganisms and investigating the mode of action underlying the cells based on contribution of lipid peroxidation and iron-dependent reaction.

Quantitative Detection of Residual E. coli Host Cell DNA by Real-Time PCR

  • Lee, Dong-Hyuck;Bae, Jung-Eun;Lee, Jung-Hee;Shin, Jeong-Sup;Kim, In-Seop
    • Journal of Microbiology and Biotechnology
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    • v.20 no.10
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    • pp.1463-1470
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    • 2010
  • E. coli has long been widely used as a host system for the manufacture of recombinant proteins intended for human therapeutic use. When considering the impurities to be eliminated during the downstream process, residual host cell DNA is a major safety concern. The presence of residual E. coli host cell DNA in the final products is typically determined using a conventional slot blot hybridization assay or total DNA Threshold assay. However, both the former and latter methods are time consuming, expensive, and relatively insensitive. This study thus attempted to develop a more sensitive real-time PCR assay for the specific detection of residual E. coli DNA. This novel method was then compared with the slot blot hybridization assay and total DNA Threshold assay in order to determine its effectiveness and overall capabilities. The novel approach involved the selection of a specific primer pair for amplification of the E. coli 16S rRNA gene in an effort to improve sensitivity, whereas the E. coli host cell DNA quantification took place through the use of SYBR Green I. The detection limit of the real-time PCR assay, under these optimized conditions, was calculated to be 0.042 pg genomic DNA, which was much higher than those of both the slot blot hybridization assay and total DNA Threshold assay, where the detection limits were 2.42 and 3.73 pg genomic DNA, respectively. Hence, the real-time PCR assay can be said to be more reproducible, more accurate, and more precise than either the slot blot hybridization assay or total DNA Threshold assay. The real-time PCR assay may thus be a promising new tool for the quantitative detection and clearance validation of residual E. coli host cell DNA during the manufacturingprocess for recombinant therapeutics.

Characterization of Bacillus species occurring anaerobic denitrification in night soil treatment

  • Park, Gyeong-Ju;Jo, Gyeong-Suk;Jeong, Eun-Ju;Yeom, Hye-Gyeong;Lee, Byeong-Heon;Lee, Min-Gyu;Kim, Jung-Gyun
    • 한국생물공학회:학술대회논문집
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    • 2003.04a
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    • pp.398-401
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    • 2003
  • Bacillus species predominantly outgrown in a night soil treatment system were isolated and characterized. Cell interactions took place among them and cell population changed under various culture conditions. Maximum removal of $NH_4\;^+-N$ and cell production occurred under the conditions of 30% DO and C/N ratio of 8. Additions of 0.8% peptone and 0.3% yeast extract to a basal medium influenced the growth of isolates and the removal of $NH_4\;^+-N$ in flask culture, and metal ions such as Ca, Fe and Mg also did. During the flask experiment of nitrogen removal under an aerobic condition, active nitrification by the isolates occurred largely in 1 h with the decrease of COD and alkalinity destructed was only 74.6% of theoretical value. From the nitrogen balance, the percentage of nitrogen lost in the flask culture was estimated to be 29.0%. This conversion of ammonia to $N_2$ under an aerobic condition was confirmed by GC analysis. The B3 process using the Bacillus species seemed to have some economic advantage.

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