• Korean Journal of Veterinary Research
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• v.33 no.4
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• pp.701-710
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• 1993

The pine needles, Pinus densiflow Sieb. et Zucc., which is a feed for goats showing a low incidence rate of cancer were evaluated to confirm the potent anticancer effects, with or without several conventional anticancer drugs. The pine needles collected from Mt. Buk-Han located near Seoul were extracted with 95% methanol and methand and concentrated. From the methanol extract, SOM-A, was extracted dichlormethane and SOM-B was extracted with ethyl acetate. SOM-C was extracted with distilled water. These extracts were tested for their antitumor activities in vitro and in vivo. Among them, SOM-A and SOM-C exhibited potent antitumor activities described as belows. 1. The cytotoxic effects of SOM-A and SOM-C were examined against in vitro cultured murine and humman tumor cells. SOM-A showed strong cytotoxicity against human tumor cell lines and SOM-C showed strong cytotoxicity against murine tumor cell lines tested. 2. The antitumor effects of SOM-A and SOM-C were examined against P388 and L1210 of mouse ascitic tumors. The highest mean survival time(MST) ration was 151%(P388) for SOM-C(90mg/kg). 3. To compare the antitumor effects of SOM-A, SOM-B, and SOM-C against solid tumors, S-180 and Ehrlich carcinoma were implanted subcutaneously to mice on Day O. The drugs were given intraperitoneally to mice once a day on Days 1-20, and the tumor weights were measured on Day 21. SOM-A showed inhibition of tumor growth more than 50% in the experiment on S-180 and Ehrlich, and SOM-C also markedly inhibited tumor growth. However, SOM-B had no effect. 4. SOM-C combined with ${\alpha}$-interferon and SOM-C combined with Mitomycin-C enhanced the antitumor activities against murine ascitic tumors P388 leukemia.

• Journal of Forest and Environmental Science
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• v.33 no.3
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• pp.187-196
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• 2017

The use of unmanned aerial vehicles (UAV) for the estimation of tree height is gaining recognition. This study aims to assess the effectiveness of tree height estimation of Pinus densiflora Sieb. et Zucc. and Pinus koraiensis Sieb. et Zucc. using digital surface model (DSM) generated from UAV-acquired imageries. Images were taken with the $Trimble^{(R)}$ UX5 equipped with Sony ${\alpha}5100$. The generated DSM, together with the digital elevation model (DEM) generated from a digital map of the study areas, were used in the estimation of tree height. Field measurements were conducted in order to generate a regression model and carry out accuracy assessment. The obtained coefficients of determination (R2) and root mean square error (RMSE) for P. densiflora (R2=0.71; RMSE=1.00 m) and P. koraiensis (R2=0.64; RMSE=0.85 m) are comparable to the results of similar studies. The results of the paired two-tailed t-test show that the two tree height estimation methods are not significantly different (p-value=0.04 and 0.10, alpha level=0.01), which means that tree height estimation using UAV imagery could be used as an alternative to field measurement.

• Journal of the Korean Society of Food Science and Nutrition
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• v.29 no.1
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• pp.6-9
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• 2000

Chemical components such as vitamins, fatty acids, amino acids and rutins in the leaves of the Pinus densiflora Sieb. et Zucc., Pinus koraiensis Sieb. et Zucc., Pinus rigida Mill., Pinus thunbergii Parl. were analyzed. The vitamin A content in the P. densiflora and vitamin C content in the P. thunbergii were obtained more than the others. Oleic acid, linolenic acid, palmitic acid and lauric acid were the major fatty acids among 19 fatty acids detected in 4 kinds of pinus leaves and the only essential fatty acid of linolenic acid was also identified. The major amino acids were glutamic acid, leucine, arginine and aspartic acid among 17 kinds of amino acids detected in the pinus leaves and the amount of essential amino acids was 47.6% in P. densiflora. The highest amount of rutin was 10.34% in P. thunbergii.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.04a
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• pp.166-166
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• 2005

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.11a
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• pp.303-303
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• 2005

• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.6
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• pp.1158-1163
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• 2001

This study was carried out to investigate the effect of ethanol extract of Pinus densiflora (PD) on the growth of lactic acid bacteria (LAB), A-1, B-9, K-7, M-7 isolated from kimchi. The growth of isolated LAB was inhibited significantly in the modified MRS broth containing 40 mg/mL PD ethanol extract. Ethyl acetate fraction showed the strongest antimicrobial activities against LAB strains compared to other fractions. The addition of PD ethanol extract to kimchi did not change the pH of kimchi greatly compare with the control during the fermentation for 25 days. Change of titratable acidity in control was more higher than in the PD ethanol extract added kimchi during fermentation. The growth of total bacteria and LAB was inhibited about 1 to 2 log cycle by the addition of PD ethanol extract during the kimchi fermentation for 25 days at 1$0^{\circ}C$. Sensory quality of PD ethanol extract added kimchi was lower than that of control.

• Journal of Korean Society for Atmospheric Environment
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• v.12 no.3
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• pp.263-268
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• 1996

This study was conducted for the assessment of the effects of acid rain and soil fertilizers on photosynthetic rate, was content, and contact angle on 5-year seedlings of Japanese red pine (Pinus densiflora Sieb. et Zucc.) leaves. The seedlings were exposed to pH 3.0 (simulated acid rain), pH 6.5 (groung water) and rain (pH around 4.6). The seedlings were also treated with $Ca(OH)_2, Mg(OH)_2, and Ca(OH)_2 + Mg(OH)_2 + C.F.(compound fertilizer)$. Photosynthetic rate, stomatal conductance, was content, contact angle value, and mineral nutrient content of the leaves were measured and the results were as follows: 1. Photosynthetic rate and stomatal conductance of the leaves increased with the increase of pH. Photosynthetic rate and stomatal conductance increased with application of soil fertilizer in the pH 3.0 treatment, but showed no changes in the rain and the pH 6.5. 2. Contact angle value and was content of the leaves did not change with the pH treatment, but increased with the fertilizer treatments. 3. Mineral nutrient contents of the leaves were lowest in the rain treatment and highest in the pH 6.5 treatment. The increase of mineral nutrient contents was observed with the soil fertilizer treatments.

• Natural Product Sciences
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• v.7 no.1
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• pp.1-4
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• 2001

The antioxidant activity of Pinus densiflora was determined by measuring the radical scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. The methanol extract of P. densiflora showed strong antioxidant activity, and thus fractionated with several solvents. The antioxidant activity potential of the individual fraction was in the order of ethyl acetate>n-butanol>water>dichloromethane> n-hexane fraction. The ethyl acetate soluble fraction exhibiting strong antioxidant activity was further purified by repeated silica gel column chromatography. Antioxidant (+)-catechin was isolated as one of the active principles from the ethyl acetate fraction, together with the inactive components, dihydrokaempferol and 1-O-benzoylglucose. The radical scavenging effect of (+)-catechin on DPPH radical exceeded to that of L-ascorbic acid which is a well known antioxidant.

• The Korean Journal of Quaternary Research
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• v.17 no.2
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• pp.139-144
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• 2003

The purpose of this study is to identify the species and to date the woods used in the Keunjung-jeon Hall, main building of the Kyungbok palace in Seoul. Samples (144) were divided into two parts, pillars (52) and other wood materials (92). Only two species were identified. They were Abies holophylla Max. and Pinus densiflora Sieb. et Zucc.Eleven among 20 Pyungju (outer pillars) were Abies holophylla, and 9 were Pinus densiflora. Seven among 12 Naejinkoju (inner pillars) were Abies holophylla, and 5 were Pinus densiflora. Three among 4 Gwikoju (inner corner pillars) were Abies holophylla, and one was Pinus densiflora. In the other wood materials, only 2 of 92 were Abies holophylla, and the others were Pinus densiflora. Tree-ring dating proved that this building was reconstructed during late 1860s. It also revealed that the old woods cut in the 17th century were reused.

• Journal of the Korea Furniture Society
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• v.12 no.2
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• pp.39-47
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• 2001

This study was carried out to identify wood species excavated at the temple site of Backjae dynasty in Neungsanri, Buyeo. At the temple site, there were great excavations such as Backjae Incense Burner(National Treasure No.287) and Stone Reliquary(National Treasure No.288). The King Chang 13 Year carved on Stone Reliquary indicated that this temple was completed in A.D 567. Identification of wood species were as follows: The Temple building was constructed by Pinus densiflora Sieb. et Zucc. The species of the main pillar of Wooden Pagoda which preserved relics of the Buddha was Zelkova serrate Makino.