• Journal of Mushroom
• /
• v.7 no.3
• /
• pp.130-134
• /
• 2009

To develop new white variety of Oyster mushroom, all white varieties which have been collected and kept in the lab were revived and screened their cultural characteristics. 84 intra-specific Oyster mushroom hybrids between the white-colored mutants Suhan and Wonhyeong were developed using hyphal anastomosis technique in 2007. The Po2007-63 ($2842-7{\times}0205-7$) was shown the best cultural characteristics, selected to be a new variety and named as 'Goni'. The new commercial strain, 'Goni' has white pilei and grows well under spring and autumn conditions in Korea. The fruiting bodies of 'Goni' are of an excellent quality in that not only the stipe is thick and long but also the pileus is small and hard. The optimum temperatures for mycelial growth and fruiting body development were $25-30^{\circ}C$ and $10-16^{\circ}C$, respectively. Time period required for the initiation of the first fruiting body is about 3 to 5 days depending on the temperatures. The shape of fruiting body is thin funnel shape. Fruiting body production per bottle was about $91{\pm}13$ g which is almost 97% quantity compared to that of other variety 'Miso'. Relatively low temperature incubation ($11^{\circ}C$) resulted in the development of better quality of 'Goni' mushrooms. When two different media including potato dextrose medium and mushroom complete medium were compared, the growth of mushroom were much faster in mushroom complete medium at $20-25^{\circ}C$, but not at $25^{\circ}C$. Similar results were observed with other variety 'Miso'. Analysis of the genetic characteristics of the new commercial strain 'Goni' showed a major DNA profile as that of the parental Suhan when primer URP 1 was used, but different to 'Miso' that was used as a control. When screens were performed with primer URP 2, DNA patterns were similar both to that of the parents and 'Miso'. This new variety of the white Oyster mushroom has a clean and fresh image that corresponds well to "health food". We therefore expect that this new strain will satisfy the consumers demand for variety and excellent mushrooms.

• Journal of Mushroom
• /
• v.7 no.3
• /
• pp.105-109
• /
• 2009

Ten to thirty percent of ginko leaf pomace(GLP) were added to pine sawdust to investigate mycelial growth. Mycelial growth on the medium with GLP was 86~101(mm/28 days) and was slower than that of the control without GLP as, 102(mm/28 days). Mycelial growth time on medium of sawdust with 10 to 20% GLP was similar to the control without GLP as 29 days but that on medium with 30% GLP was delayed for 2 days. The time of pinhead formations of sawdust with 10 to 20% GLP was 7 days, the growth time to harvest was 11 days. These results were similar to those of the control without GLP. The sizes of pileus of treatments with 10 to 30% GLP were tend to be smaller compared with the control. The length of stipe of 10% GLP treatment was 98mm which was longer than that of the control with 91mm. The thickness of stipes of 10 to 30% GLP treatments were 45 to 48mm which were tend to be thicker than that of the control with 45mm. The hardness of stipe were 35,062($g/cm^2$) to 4,1065($g/cm^2$)) which were harder than that of the control with 32,156($g/cm^2$)). GLP treatments seem to be favorable to store or distribute. Yields per volume of GLP added treatments were 88.7g to 95.6g, which was increased 3 to 12% compare to 85.8g of the control without GLP.

• Journal of Mushroom
• /
• v.11 no.3
• /
• pp.154-158
• /
• 2013

To develop a new variety of oyster mushroom(Pleurotus ostreatus), parental strains was selected by the method of Mon-Mon crossing between monokaryotic strains derived from ASI 2596(Suhan No.3) and ASI 2782(Black pileus mutant). The SB-73(ASI 2596-11 x 2782-8) was shown the best cultural characteristics, selected to be a new variety and named as 'Dagul'. The 'Dagul' was formed incompatibility line distinctly in the confrontation growth of parental strains Suhan No.3 and ASI 2782. The optimum temperature for mycelial growth, fruiting body development and pH arrange were $25{\sim}30^{\circ}C$, $14{\sim}17^{\circ}C$ and pH5~8, respectively. Fruiting body production per bottle was about $68.0{\pm}24.1$ g which is almost 115% quantity compared to that of other variety Suhan No.3. And also the stipe is long and individual generation is multiple. Analysis of the genetic characteristics of the new variety 'Dagul' showed different DNA bands as that of the control strains, Suhan No.3 and ASI 2782, when RAPD(Random Amplified Polymorphic DNA) primers URP7 and Rcb1 were used. This new variety 'Dagul' of oyster mushroom is characterized by multiple of individual generation and the stipe is long. We therefore expect that this new strain will increase of the income by cultivation of field.

• Food Science and Preservation
• /
• v.10 no.4
• /
• pp.476-481
• /
• 2003

As its high functional properties to be used as medicine or food, the cultivation of Agaricus mushroom has been expanded and iぉ commercialization required better storage methods that can extend its functional and nutritional value for longer period. We selected drying as the most plausible method to meet such requirement, and several drying conditions were investigated to locate the optimum drying condition that can be used to keep the quality of mushroom. Drying temperature of 50$^{\circ}C$, 60$^{\circ}C$, 100$^{\circ}C$ were selected to trace the drying time required to achieve the moisture content of mushrooms less than 10%. The drying temperature at 50$^{\circ}C$ required 29 hrs of drying time, while 100$^{\circ}C$ required only 10 hrs of drying tune. However, their quality characteristics on the following categories, on the degree of browning and color were investigated to find the optimum drying condition. In addition, sensory evaluation was conducted to evaluate the quality of dried mushrooms produced by each drying condition. The browning of the mushroom was evidently increased as the higher drying temperature was used and 50$^{\circ}C$ drying produced the most desirable quality of all in pileus or stipe. The aeon of browning intensified by drying temperature was comparable to the result of whiteness index value, which resulted lower L values as drying temperature increased. and the 50$^{\circ}C$ drying resulted the most highest L values among all drying samples. As the browning and whiteness results implied, the sensory evaluation result gathered from the present research indicated that the 50$^{\circ}C$ drying was the most favorable drying condition by scoring the most highest average scores on flavors, color, appearance, and overall acceptability conducted by the 10 evaluation panels.

• The Korean Journal of Mycology
• /
• v.31 no.3
• /
• pp.134-140
• /
• 2003

Wild Mushrooms collected in Mt. Halla of Jeiu Island around altitude of $200{\sim}1000\;m$ from 1999 to 2000 were studied to investigate utilization as the natural breeding sites by wild drosophilids. Thirty-five mushroom species belonging to the 19 genera of 12 families from 2 orders were used as natural breeding sites by drosophilids. Adult flies emerged from 14 species of mushrooms belonging to the 11 genera of 8 families from 2 orders were identified. From the rest of 21 species of mushrooms, only larvae or/and pupae were reaped. Among 35 species of mushrooms, 34 species belonged to order Agaricales and only one species, Sarcodon scabrosum, belonged to order Aphyllophorales. In terms of composition of mushroom families, total 35 species belonging to 12 families were composed of 10 species of Russulaceae, 6 species of Amanitaceae, 4 species of each Tricholomataceae and Agaricaceae, 3 species of Boletaceae, 2 species of Strophariaceae and each one species of rest 6 families. Concerning the composition of the genus, 35 species belonging to 11 genera were composed of 8 species of Russula, 6 species of Amanita, 4 species of Agaricus, 2 species of Lactarius, and each one species of rest 7 genera. In terms of the mushroomsize preference of drosophilid flies for breeding sites, most flies preferred comparatively large mushrooms above 5 cm of pileus size in diameter, Fourteen species of drosophilids were identified as mushroom-breeding species. Among these, one and five species belonged to genera Mycodrosophila and Hirtodrosophila respectively, The genus Drosophila included the most abundant 8 species. Drosophila bizonata and Hirtodrosophila sexvittata bred on 6 species of mushrooms respectively, D. unispina bred on 3 species of mushrooms, rest species of Drosophila bred on one or two species of mushrooms. From the result of this study and other literatures, it could be presumed that much more mushroom species could serve as the natural breeding sites for greater number of drosophilid species than those examined in this study on Jeju Island.

• The Korean Journal of Mycology
• /
• v.31 no.3
• /
• pp.192-199
• /
• 2003

This study was carried out to investigate the artificial cultivation of Pleurotus eryngii on the optimal medium vessel, periods of cultivation and the optimal method of pinheading for both yield and quality of fruiting body were also performed. The optimal composition of sawdust medium in polypropylene(PP) bottle was combination of sawdust(70%) and corncob(30%) but increased amount of corncob delayed the period of mycelial growth. The mycelial growth and the yield of fruiting body in the medium with beat pulp were worse than that without beat pulp. The optimal composition of nutrients for both yield and quality of fruit body tuned out to be a combination of rice bran(12%), wheat bran(12%) and cottonseed cake(6%). Additions of zeolite, shell lime and bean curd dregs were not effective in mycelial growth and yield of fruit body. When testing size of PP bottle for cultivation, the larger of bottle mouth is, the more pinheading number found, but the number of available fruit body is not significantly different. The culture in $1100\;ml-{\phi}75\;mm$ bottle is the best in the yield and quality of fruit body than those in $555\;ml-{\phi}50\;mm,\;850\;ml-{\phi}58\;mm,\;850\;ml-{\phi}65\;mm\;and\;1100\;ml-{\phi}65\;mm$ bottle. Using the PP bag for cultivation, a square shaped bag was better than a round shaped and black square shaped in mycelial growth and yield of fruit body. The most suitable period of incubation was 35 days after inoculation at $22{\pm}2^{\circ}C$. When the incubation periods was decreased less than 35 days, the pileus formation and yields were very bad but a pinheading condition looked similar, For an optimal pinheading, turning upside down was better than standing and covering.

• Journal of Mushroom
• /
• v.19 no.3
• /
• pp.166-175
• /
• 2021

In this study, monokaryons of "Heukari" (Pleurotus ostreatus) and "Hosan" (Pleurotus pulmonarius) were separated to remove the cell wall, and a cross-species protoplast fusion was developed through chemical treatment with polyethylene glycol. The protoplast-fused PF160306 and PF160313 strains have a culture period of 10 and 2 days shorter than that of the "Heuktari" and "Hosan" cultivars, respectively. Furthermore, the growth of the strains was faster than that of the existing cultivars. The yield was 135.9 g per bottle, which was approximately 8% higher than that of the commercially available "Hosan" cultivar; however, it was not statistically significant. A growth survey was conducted after treatment at five temperatures (15, 18, 21, 23, and 25℃). The growth of the strains accelerated with the increase in temperature. However, at 21℃, the yellow color of pileus was the brightest. Band pattern, assessed using URP Primer 7, was similar to that of the "Hosan" cultivar. The DPPH radical scavenging capacity and polyphenol content were 62.5% and 43.5 mg/mL, respectively, for "Sunjung" and 65.7% and 49.9 mg/mL, respectively, for PF160313. Furthermore, the antihypertensive activities of the "Sunjung" cultivar and PF160313 were similarly high at 74% and 75%, respectively. In conclusion, cross-species hybridization via the protoplast fusion technique can be used for obtaining primary data for mushroom breeding to develop new varieties. In addition, the protoplast fusion technique might aid in expanding the market for yellow mushrooms.

• Journal of Mushroom
• /
• v.17 no.3
• /
• pp.119-125
• /
• 2019

This study aims to report the results for the analysis of the growth environment by applying smart farm technology to "Chunchu No 2" farmers in order to develop an optimal growth model for precision cultivation of bottle-grown oyster mushrooms. The temperature, humidity, carbon dioxide concentration, and illumination data were collected and analyzed using an environmental sensor installed to obtain growth environment data from the oyster mushroom cultivator. Analysis of the collected temperature data revealed that the temperature at the time of granulation was $19.5^{\circ}C$ after scraping, and the mushroom was generated and maintained at about $21^{\circ}C$ until the bottle was flipped. When the fruiting body grew and approached harvest time, mushrooms were harvested while maintaining the temperature between $14^{\circ}C$ and $18^{\circ}C$. The humidity was maintained at almost 100% during the complete growth stage. Carbon dioxide concentration gradually increased until 3 days after the beginning of cultivation, and then increased rapidly to almost 5,500 ppm. From the 6th day, carbon dioxide concentration was gradually decreased through ventilation and was maintained at 1,600 ppm during harvest. Light intensity of 8 lux was irradiated up to day 6 after seeding, and growth was then continued while periodically irradiating 4 lux light. The fruiting body characteristics of "Chunchu No 2" cultivated in the farmhouse were as follows: pileus diameter of 26.5 mm and thickness of 4.9 mm, stipe thickness of 8.9 mm, and length of 68.7 mm. The fruiting body yield was 166.8 g/850 ml, and the individual weight was 12.8 g/10 units.

• The Korean Journal of Mycology
• /
• v.51 no.2
• /
• pp.81-90
• /
• 2023

The cultivation method of shiitake is divided into two methods: log cultivation and sawdust cultivation. Recently, the importance of sawdust cultivation has been highlighted due to problems such as environmental problems, rising labor costs, and a reduced labor force. Among the 24 Sawdust-cultivated Lentinula edodes cultivars in Korea stored by the National Institute of Forest Science, this study was conducted to select excellent cultivars under the sawdust block cultivation method. After inoculation, the sawdust mediums were cultured for 100 days (60 days in dark and then 40 days in light), and only Sanjo 712ho sprouted the primordia on the 10^th day of light culture (total 70 days of cultivation). As a result of cultivation, the average total yield of the 24 cultivars were 1,816 g and Sanjo 712ho was 2,267 g. The fruiting body yield was the highest in Sanjo 713ho with 3,443 g followed by Sanjo 710ho (3,355 g). Sanlim 10ho, Sansanhyang, and Sanjo 716ho showed low production with 174, 238, and 214 g, respectively. As a result of investigating the morphological characteristics of the fruit bodies, the fresh weight of Sansanhyang and Bambithyang was about twice as heavy as the overall average, and Sulbaekhyang was about twice as light. Bambithyang was the largest and longest in diameter and length of the stipe, and the thickness of the stem was the thickest in Sanjo 716ho. As for the hardness of pileus, Bambithyang showed the highest value at 1,276 g/5 mm, and Sanjo 711ho showed the lowest value at 542 g/5 mm. In summary, Bambithyang showed the best fruiting body characteristics and Sanjo 713ho showed the highest yield in the sawdust block cultivation method. This study, the cultivar with shortened cultivation periods and cultivars with excellent morphological characteristics and high yields.

• Journal of the Korean Society of Food Culture
• /
• v.21 no.3
• /
• pp.297-302
• /
• 2006

This study has examined the physicochemical properties of the Pleurotus eryngii, including their proximate components, amount mineral content, total dietary fiber, total sugar, reducing sugar and free sugar. Additionally, it measured the P. egii ethanol extracts and the total amounts of polyphenol compounds as well as its electron donating ability (EDA) of the substance fraction (SF). The P. eryngii powder's moisture content was 9.0% and each of the other element content such as carbohydrate, crude protein, crude ash and crude fat was found to be 63.06%, 20.70%, 5.20% and 2.0% respectively. Potassium (K) was shown to be the greatest inorganic content and manganese (Mn) was the lowest. Furthermore fructose, galactose, glucose lactose and maltose free sugar content was found in this order. 387 mg% of the total amounts of polyphenol was found in the P. eryngii whole body ethanol extract, 158 mg% in the stipe extract, 593 mg% in the pileus extract and 607 mg% in the substance fraction (SF). Electron donating ability (EDA) was highest at 91.12% in the whole body extract and lowest at 62.90% in the stipe extract. Additionally, the EDA for substance fraction (SF) 0.02%-0.1% was found to be 57.78-77.33%, which was lower than the 0.02%-tocopherol (93.92%) and BHT (96.72%). From these results, it can be assumed that P. eryngii offers superior antioxidative effects with its high content of fiber, inorganic materials and total amounts of polyphenol as well as high electron donating ability (EDA), thereby making it ideal for use in functional foods and industrial materials.