• Journal of Veterinary Science
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• v.21 no.3
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• pp.36.1-36.9
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• 2020

Background: Pseudorabies, also known as Aujeszky's disease, is caused by the pseudorabies virus (PRV) and has been recognized as a critical disease affecting the pig industry and a wide range of animals around the world, resulting in great economic losses each year. Shandong province, one of the most vital food animal-breeding regions in China, has a very dense pig population, within which pseudorabies infections were detected in recent years. The data, however, on PRV epidemiology and coinfection rates of PRV with other major swine diseases is sparse. Objectives: This study aimed to investigate the PRV epidemiology in Shandong and analyze the current control measures. Methods: In this study, a total number of 16,457 serum samples and 1,638 tissue samples, which were collected from 362 intensive pig farms (≥ 300 sows/farm) covered all cities in Shandong, were tested by performing enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR). Results: Overall, 52.7% and 91.5% of the serum samples were positive for PRV-gE and -gB, respectively, based on ELISA results. In addition, 15.7% of the tissue samples were PCR positive for PRV. The coinfection rates of PRV with porcine circovirus type 2 (PCV2), porcine reproductive and respiratory syndrome virus, and classical swine fever virus were measured; coinfection with PCV2 was 35.0%, higher than those of the other two viruses. Macroscopic and microscopic lesions were observed in various tissues during histopathological examination. Conclusions: The results demonstrate the PRV prevalence and its coinfection rates in Shandong province and indicate that pseudorabies is endemic in pig farms in this region. This study provides epidemiological data that can be useful in the prevention and control of pseudorabies in Shandong, China.

• Korean Journal of Veterinary Service
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• v.38 no.2
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• pp.89-94
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• 2015

Respiratory disease in pigs is common in modern pork production worldwide and is often referred to as porcine respiratory disease complex (PRDC). PRDC is polymicrobial in nature, and results from infection with various combinations of primary and secondary respiratory pathogens. The control of swine respiratory disease requires an understanding of the interactions between the organisms that can cause this illness, the pig and management of the environment. This study was carried out to investigate the lesion of red internal organs in slaughtered pigs and provided assistant data for pig farms. A total of 900 lung samples, 45 farms were collected randomly from slaughtered pigs in Jeonbuk province from April to December in 2014. Gross lesions such as swine enzootic pneumonia (SEP), pleuritis, pleuropneumonia, pericarditis, liver white spots were examined for the pigs. Overall prevalence of SEP was 70.8%. According to season, the incidence occurred higher in summer than winter, fall and spring. The mean SEP score was 1.4, the highest incidence occurred in fall. The prevalence of pleuropneumonia, pleuritis, pericarditis, and milk spot was 26.1%, 71.4%, 2.8%, 21.6%, respectively. In the detection of pathogens, PRRS was not detected, PCV2 was positive in 87.6%.

• Korean Journal of Veterinary Service
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• v.33 no.4
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• pp.345-351
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• 2010

Toxoplasma gondii is a species of parasitic protozoa in the genus Toxoplasma. The definitive host of T. gondii is the cat, but the parasite can be carried by the vast majority of warm-blooded animals, including humans. It is often found in the tissues of food animals including pigs and sheep. To determine the regional prevalence of infection with T. gondii, bloods (n=300) from domestic pigs and tissues (n=200) from slaughter pigs in Gyeongnam province were tested using an enzyme-linked immunosorbent assay (ELISA) and a polymerase chain reaction (PCR) for detection of antibody and antigen. A total of 115 sero-positive pigs were identified for a prevalence rate of 38.3%. Of the 50 herds from domestic pigs tested, 34 had at least one sero-positive pig for a herd prevalence rate of 68.0%. Sero-positive rates of pigs in fattening farm were higher than that of pigs in breeding company. Sero-positive rates of sows were higher than that of growing pigs. Seasonally, sero-positive rates of pigs were highest in winter (80.0%) and lowest in spring (23.8%). According to farm size, sero-positive rates of pigs were higher in small size farms (${\leq}$2,000) than that of big size farms (>2,000). However, none of the bloods (n=300) from domestic pigs and tissues (n=200) from slaughter pigs were positive for T. gondii specific DNA by PCR.

• Korean Journal of Organic Agriculture
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• v.25 no.2
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• pp.489-498
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• 2017

This study was conducted to compare the mortality number of swine between before and after HACCP implementation on swine farm. Also, we analyzed the reason for implementing HACCP system, advantage and disadvantage of HACCP system implemented swine farm. The study was carried out by randomly selected fifty swine farms located in all around Korea. The results were as follows: The mortality number of swine before HACCP system was found to be 288.30, 122.90, 91.08, 18.22, and 108.10 in respiratory, diarrhea, abortion (stillbirth), an accidental death and others, respectively. However, after HACCP implementation, it decreased, without significantly, to 261.60, 101.10, 85.91, 16.37, and 108.60, respectively. Therefore, Therefore, the total number of mortality pig decreased from 628.70 (before HACCP) to 573.60 (after HACCP). The enhancement of farm competitiveness (26.92%) and hygienic and safety pig production (23.43%) were ranked as the first and second proposes for implementing HACCP system on swine farm. The major advantage of implementing HACCP were improvement the farm sanitation management level (20.90%) and the major disadvantage was surveyed as HACCP records (23.10%). We are expecting that our results might be used for producing safer livestock products by improving livestock products HACCP policy.

• Journal of Animal Environmental Science
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• v.20 no.1
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• pp.1-8
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• 2014

The study on design criteria of solid-liquid separator for dairy farms was done by testing various screens, presses, and RPMs of screw auger with remodeled screw-press type pig-manure separator. The moisture content of separated solid increase from 68.3% to 74.2% as auger rotating speed increased from 9.8 to 29.2 RPM at 34.8 kPa of pressure and 1.0 mm of slit wedge wire screen condition. The moisture contents of separated solid were 72~77%, work efficiencies were 16~18 kg/min at 1.0 mm of slit wedge wire screen. The efficiency was higher than one at 0.5 mm of slit wedge wire screen, which was used to separate for pig manure. The best work efficiency was 18 kg/min at 42.5 kPa, 1.0 mm slit screen. The separated solid moisture content was 75% at this condition.

• Journal of Animal Science and Technology
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• v.65 no.2
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• pp.365-376
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• 2023

Pig breeding management directly contributes to the profitability of pig farms, and pregnancy diagnosis is an important factor in breeding management. Therefore, the need to diagnose pregnancy in sows is emphasized, and various studies have been conducted in this area. We propose a computer-aided diagnosis system to assist livestock farmers to diagnose sow pregnancy through ultrasound. Methods for diagnosing pregnancy in sows through ultrasound include the Doppler method, which measures the heart rate and pulse status, and the echo method, which diagnoses by amplitude depth technique. We propose a method that uses deep learning algorithms on ultrasonography, which is part of the echo method. As deep learning-based classification algorithms, Inception-v4, Xception, and EfficientNetV2 were used and compared to find the optimal algorithm for pregnancy diagnosis in sows. Gaussian and speckle noises were added to the ultrasound images according to the characteristics of the ultrasonography, which is easily affected by noise from the surrounding environments. Both the original and noise added ultrasound images of sows were tested together to determine the suitability of the proposed method on farms. The pregnancy diagnosis performance on the original ultrasound images achieved 0.99 in accuracy in the highest case and on the ultrasound images with noises, the performance achieved 0.98 in accuracy. The diagnosis performance achieved 0.96 in accuracy even when the intensity of noise was strong, proving its robustness against noise.

• Korean Journal of Veterinary Service
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• v.46 no.4
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• pp.325-334
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• 2023

Edema disease (ED) in pigs is enterotoxemia caused by Shiga toxin type 2e (Stx2e)-producing Escherichia coli (STEC) and frequently occurs in young piglets. Therefore, ED causes enormous economic losses in pig farms. In this study, a modified Stx2e (mStx2e) antigen was expressed and purified using commercial E. coli expression system. Monoclonal antibody was serviced by Ynto Ab Inc., using Phage Display Technique. Anti-Stx2e antibodies in piglets were measured by indirect ELISA using mStx2e antigens. Naive Stx2e in piglets were detected by Sandwich ELISA using Stx2e-monoclonal antibodies and commercial Stx2e-polyclonal antibodies. Among 3,480 piglets, anti-Stx2e antibodies were observed in 2,573 piglets. The 49.4% among 830 piglet serum samples possessed 0.625 ㎍/mL or more of Stx2e proteins. The 18.3% of 830 sera had 0.313 ㎍/mL of Stx2e proteins. The 32.3% of 830 samples held 0.156 ㎍/mL or less of Stx2e proteins. These results show that indirect ELISA using mStx2e antigen and Sandwich ELISA using Stx2e-monoclonal and polyclonal antibodies can be useful to detect ED in piglets.

• Journal of Animal Science and Technology
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• v.65 no.4
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• pp.856-864
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• 2023

There are a variety of microorganisms in the animal intestine, and it has been known that they play important roles in the host such as suppression of potentially pathogenic microorganisms, modulation of the gut immunity. In addition, the gut microbiota and the livestock growth performance have long been known to be related. Therefore, we evaluated the interrelation between the growth performance and the gut microbiome of the pigs from 3 different farms, with pigs of varied ages ready to be supplied to the market. When pigs reached average market weight of 118 kg, the average age of pigs in three different farms were < 180 days, about 190 days, and > 200 days, respectively. Fecal samples were collected from pigs of age of 70 days, 100 days, 130 days, and 160 days. The output data of the 16S rRNA gene sequencing by the Illumina Miseq platform was filtered and analyzed using Quantitative Insights into Microbial Ecology (QIIME)2, and the statistical analysis was performed using Statistical Analysis of Metagenomic Profiles (STAMP). The results of this study showed that the gut microbial communities shifted as pigs aged along with significant difference in the relative abundance of different phyla and genera in different age groups of pigs from each farm. Even though, there was no statistical differences among groups in terms of Chao1, the number of observed operational taxonomic units (OTUs), and the Shannon index, our results showed higher abundances of Bifidobacterium, Clostridium and Lactobacillus in the feces of pigs with rapid growth rate. These results will help us to elucidate important gut microbiota that can affect the growth performance of pigs.

• Korean Journal of Organic Agriculture
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• v.25 no.3
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• pp.643-651
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• 2017

The purpose of this study was to analyze the purpose, effectiveness and problems of HACCP system implementation for small, medium and large scale of swine farms implementing HACCP. Small and medium scale swine farms stated that the primary purpose of HACCP implementation was to receive government funding, but large scale swine farm was answered to improve the farm's competitiveness. In the case of small farms, the effect of HACCP implementation responded to systematic farm management as the top priority, while mid-scale and large-scale swine farms were said to improve farm sanitation. In addition, the biggest problems caused by the implementation of HACCP were the difficulty of improving the consciousness of the employees (small farm) and the records management (medium scale, large farm) by HACCP implementation. In order to solve these problems, it is necessary to adjust the number of HACCP evaluation items of pig farm and HACCP education for employees.

• Journal of the Korean Data and Information Science Society
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• v.27 no.2
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• pp.429-435
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• 2016

Heritability estimates for daily gain (g), backfat thickness (mm), days to 90kg (day), loin eye depth (mm) and meat percent (%) were 0.40, 0.44, 0.40, 0.25 and 0.48, respectively. Estimates of correlation between breeding value and rank for meat productivity traits by model 1 and 2 were 0.995 1.000 and 0.991 1.000, respectively and highly significant (p< 0.0001), and they were almost identical to the breeding values estimated by different farms. When top 5% and top 10% animals were selected by meat productive traits at different farms, markedly different animals were selected by farms since the selected improvement traits in each farm maintaining closed herds were different. Therefore, it seems to be desirable that superior pigs should be selected after the establishment of evaluation system for genetic performance at national level using the integrated data obtained from various farms.