• Proceedings of the Korean Magnestics Society Conference
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• 2012.11a
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• pp.57-58
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• 2012

이 연구는 BLS 실험을 이용하여 $Co_{40}Fe_{40}B_{20}$ 박막의 자성특성을 연구하였다. CoFeB의 열처리 온도에 따라서 $4{\pi}MS$는 일정하였고, $A_{ex}$는 $300^{\circ}C$ 이상에서 열처리한 시료의 경우 비슷한 값을 가짐을 확인하였다.

• Journal of Life Science
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• v.25 no.11
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• pp.1331-1337
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• 2015

S-allylcysteine (SAC) is an aged garlic derived water soluble organosulfur compound and has been suggested to have anticarcinogenic activity against diverse types of cancer cells. This review summarizes the cellular signaling pathways and molecular mechanisms whereby SAC exerts its effects on cellular proliferation, apoptosis, cell cycle progression and metastasis based on the results from both in vitro and in vivo studies. SAC activates proapoptotic proteins including Bax and caspase-3, but suppresses antiapoptotic Bcl-2 family proteins to bring about cancer cell death through mitochondria-mediated intrinsic pathway. SAC also inhibits cellular proliferation by inducing cell cycle arrest in which SAC reduces expression and activation of NF-κB, cyclins, Cdks, PCNA and c-Jun, but elevates expression of cell cycle inhibitor proteins p16 and p21 through suppression of both PI3K/Akt/mTOR and MAPK/ERK signaling pathways. And, SAC inhibits invasion and metastasis of cancer cells by inducing suppression of both angiogenesis and epithelial-mesenchymal transition (EMT) through decreased cyclooxygenase (COX)-2 expression and increased E-cadherin expression which were then caused by suppression of inhibitory transcription factors Id-1 and SLUG from SAC-mediated inactivation of both MAPK/ERK and PI3K/Akt/mTOR/NF-κB signaling pathways. Furthermore, SAC prevents toxic compound-induced carcinogenesis by inducing antioxidant enzymes such as glutathione-s-transferase (GST). Thus, SAC can be considered as a potential chemotherapeutic agent for the prevention and treatment of cancer.

• Korean Journal Plant Pathology
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• v.3 no.3
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• pp.174-179
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• 1987

Four thousand eight hundred and eighty five isolates of Pyricularia oryzae were obtained from the diseased rice specimens collected from various areas of Korea for race identification during 1978 - 1985. A total of 15 races inculding 6 races pathogenic to Tongil lines, 2 T-races, 5 C-races and 2 N-races was identified using a old Japanese differential set during 1978-1980. Since 1981, number of races identified by a Korean differential set was 18 races which were composed of 11 KI-races pathogenic to either Tongil lines or Japonica cultivars and 8 KJ-races pathogenic to only Japonica cultivars. The prevalent race was $N-2^{+t}$ during 1978-1979 and race KJ-301 since 1980, respectively. Races KI-315a and KI-315b pathogenic to most of Tongil lines were identified in 1983. being widely distributed to date. Races KJ-105 and KJ-201 pathogenic to the cultivars possessing resistance genes Pi-k and Pi-i were prevalent in Gangweon province, whereas race KI-315b was prevalent in Chungbug and Jeonnam districts.

• Applied Biological Chemistry
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• v.34 no.2
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• pp.134-141
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• 1991

In order to investigate the changes and characteristics of biochemical metabolic substances of soybean tissue culture during the cultural period, immature cotyledons were detached form the plant on 15th days after flowering and cultured in vitro for 3 weeks. The cultures were classified into embryogenic(EC) and non-embryogenic callus(NEC). A part of the EC lines were subcultured for another 3 weeks and classified into root forming(RFC), and shoot forming cultures(SFC). Another part of the EC lines were used for isolation of protoplasts, which were subsequently cultured in vitro for 4 weeks. The cultures were classified into embryogenic(PEC) and non-embryogenic callus(PNEC) derived from the protoplasts. The cultures of EC and PEC lines showed higher phenylalanine content and lower methionine content than those of NEC and PNEC. At organ differentiation stage, both cultures showed the content of aspartic acid decreased, while the other amino acids increased as a whole. The protein pattern analysis of the cultures revealed that EC and NEC lines contained distinctive polypeptides, with mass of ca. 18KD for EC and ca. 22KD for NEC respectively. The EC and PEC lines also showed high activity of peroxidase isozyme A(piA), while the RFC and SFC lines showed that of peroxidase isozyme B(piB).

• BMB Reports
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• v.39 no.1
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• pp.97-104
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• 2006

Endostatin is a tumor-derived angiogenesis inhibitor, and the endogenous 20 kDa carboxyl-terminal fragment of collagen XVIII. In addition to inhibiting angiogenesis, endostatin inhibits tumor growth and the induction of apoptosis in several endothelial cell types. However, the mechanisms that regulate endostatin-induced apoptotic cell death are unclear. Here, we investigated apoptotic cell death and the underlying regulatory mechanisms elicited of endostatin in human umbilical vein endothelial cells (HUVECs). Endostatin was found to induce typical apoptotic features, such as, chromatin condensation and DNA fragmentation in these cells. Thus, as the phosphoinositide 3-OH kinase (PI3K)/protein kinase B (PKB) signaling pathway has been shown to prevent apoptosis in various cell types, we investigated whether this pathway could protect cells against endostatin induced apoptosis. It was found that the inhibition of PI3K/PKB significantly increased endostatin-induced apoptosis, and that endostatin-induced cell death is physiologically linked to PKB-mediated cell survival through caspase-8.

• Journal of the Korean Applied Science and Technology
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• v.14 no.2
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• pp.87-91
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• 1997

서로 다른 씨클로펜타디엔 유도체가 ${\pi}$-결합된 최초의 비스무스 화합물인 ${\eta}^5-C_5(CH_3)_5Bi({\eta}^5-C_5H_5)_2$이 디펜타메틸씨클로펜타디에닐 비스무스디메틸아미드 $Cp{\ast}_2BiNMe_2[Cp{\ast}=C_5(CH_3)_5]$와 씨클로펜타디에닐 모노머와의 반응으로 합성되었다. 반응조건은 에테르 용매하에 -78$^{\circ}C$ 반응온도 조건하에서 얻어졌다. 합성된 반응물을 노르말 헥산 용매에서 재결정시킨 결과, 검은색 결정이 60% 수율로 얻어졌다. 그리고 재결정시킨 반응물을 190K에서 X-선 단결정 구조 분석 방법에 의해 그 구조를 밝혔다. 그 결과 결정계의 격자계는 I2/a, a=1756.00 picometer, b=906.00 picometer, c=2211.00 picometer, ${\beta}$=104.04, Z=8로 확인되었다. 여기서 a, b, c는 결정�Ю� 상수이고, ${\beta}$는 결정격자 상수인 b와 c간의 각도이며, Z는 단위 결정 격자당 분자의 갯수이다.

• The Plant Pathology Journal
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• v.19 no.1
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• pp.57-63
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• 2003

An insertion sequence identified as a solo long terminal repeat (LTR) of a new rice copia-like retrotransposon was detected in the ORE of the Pi-b gene from the rice cv. Nipponbare, and was designated as Osr1. Osr1 consists of a 6386 bp nucleotide sequence including 965 bp LTRs on both ends with an 82％ nucleotide sequence identity to the wheat Tarl retrotransposon on reverse transcriptase. Nucleotide divergence was noted among the individual LTRs, as well as the coding region of Osr1. Various restriction fragment length polymorphism (RFLP) of LTR were detected in indica cultivars, whereas, only a few could be detected in the japonica cultivars. The population of Osr1 is lower in the wild-type rice compared with that in the domesticated cultivars. The insertion of LTR sequence in the h-b gene in the susceptible cultivar suggested that retro-tyansposon-mediated insertional mutation might play an important role in the resistance breakdown, as well as in the evolution of resistance genes in rice.

• Korean Journal of Food Science and Technology
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• v.11 no.2
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• pp.118-121
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• 1979

Under the experimental conditions of varying the thickness(2L cm) of ginseng disc and the bulk, concentration $(C_b.{\;}%)$, the experimental results were well coincided with those of the model which was set up by using the inter disc diffusion with constant diffusivity. It was therefore convinced that the extraction rate is controlled by the diffusion rate of ginseng components through the disc. The relationship between the diffusivity$(D{\;}cm^2/min)$ and the mean concentration (C %) of soluble ginseng components in the disc is as follows, $ln{\frac{C-C_{b0}}{C_i-C_{b0}}}=ln{\frac{8}{{\pi}^2}-{\frac{{\pi}^2D}{4L^2}{\theta}$ where $C_i(%)$ is the initial concentration of soluble ginseng components and $\theta$ (min.) is the extraction time.

• Genomics & Informatics
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• v.20 no.1
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• pp.7.1-7.13
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• 2022

2-Methoxy-1,4-naphthoquinone (MNQ) has been shown to cause cytotoxic towards various cancer cell lines. This study is designed to investigate the regulatory effect of MNQ on the key cancer genes in mitogen-activated protein kinase, phosphoinositide 3-kinase, and nuclear factor κB signaling pathways. The expression levels of the genes were compared at different time point using polymerase chain reaction arrays and Ingenuity Pathway Analysis was performed to identify gene networks that are most significant to key cancer genes. A total of 43 differentially expressed genes were identified with 21 up-regulated and 22 down-regulated genes. Up-regulated genes were involved in apoptosis, cell cycle and act as tumor suppressor while down-regulated genes were involved in anti-apoptosis, angiogenesis, cell cycle and act as transcription factor as well as proto-oncogenes. MNQ exhibited multiple regulatory effects on the cancer key genes that targeting at cell proliferation, cell differentiation, cell transformation, apoptosis, reduce inflammatory responses, inhibits angiogenesis and metastasis.

• 한국체육학회지인문사회과학편
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• v.51 no.3
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• pp.363-372
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• 2012

One of the major ocular complications of diabetes mellitus(DM) is retinopathy, which is characterized by increased neovascularization and neural degeneration in the retina. In the present study, we investigated the effects of treadmill exercise on retinopathy in the rats with DM. Thirty-two male Sprague-Dawley rats were divided into four groups(n = 8 in each group): control group, exercise group, DM-induction group, and DM-induction and exercise group. DM was induced by intraperitoneal injection of streptozotocin. The rats in the exercise groups were made to run on the treadmill for 30 min five times per a week, during 12 weeks. The expressions of phosphoinositide 3-kinase(PI3K), phospho-protein kinase B(pAkt), hypoxia inducible factor-1α(HIF-1α), and vascular endothelial growth factor(VEGF) in the retina were determined using western blot analysis and immunohistochemistry. In the present results, the expressions of PI3K, pAkt, HIF-1α, and VEGF in the retina of the diabetic rats were increased. Treadmill exercise suppressed HIF-1α and VEGF expressions through inhibition of PI3K/pAkt pathway in the diabetic rats. These results suggest that treadmill exercise may ameliorate the progression of diabetes-induced retinopathy by inhibiting neovascularization in the retina.