• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2000년도 추계 학술발표회
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• pp.12.2-12
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• 2000

• The Plant Pathology Journal
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• 제15권1호
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• pp.28-33
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• 1999

Phytophthora root rot of Chniese cabbage and spinach is reported for the first time in Korea. The diseases ocurred at Yangju, Seosan and Yeocheon in Korea from 1995 through 1998, mainly in lowland and submerged areas. Symptoms consisted of stunt, yellows, wilt and eventual death due to root rot. Fourteen isolates collected from naturally infected plants were all identified as P. drechsleri based on mycological characteristics. PCR-RFLP analysis of rDNA of the isolates confirmed the above result, since the restriction band patterns of the small subunit and internal transcribed spacers were identical to P. drechsleri and P. cryptogea, but distinct from closely related species of P. erythroseptica, P. cambivora, P. sojae and P. megasperma. The pathogen showed strong pathogenicity to Chinese cabbage, moderate to spinach, radish, cabbage and tomato, and weak or none to brown mustard, kale, chicory and pepper in pathogenicity tests.

• The Plant Pathology Journal
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• 제19권6호
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• pp.288-290
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• 2003

Shoot blight of suckers of common lilac plants (Syringa vulgaris) was found in an apartment garden in Daegu, Korea. The causal organism was a species of Phytophthora, which was homothallic with paragynous antheridium. Oogonia were globose and measured 21.5-34.0 $\mu\textrm{m}$ with an average of 29.7$\pm$3.2 $\mu\textrm{m}$ in diameter. Oospores in oogonia were round, light orange brown when mature, mostly plerotic, and measured an average of 25.6$\pm$3 $\mu\textrm{m}$ in diameter. Antheridia were ovoid and around 11.7$\times$7.8 $\mu\textrm{m}$. Sporangia seldom formed on agar media but formed abundantly in water. Sporangia that formed in water were semipapillate, ovoid obpyriform, non-caducous, and 23.4-66.3$\times$17.6-37.1 $\mu\textrm{m}$ in dimension with I/b ratio of 1.46. The causal organism was identified as Phytophthora citricola Sawada on the basis of morphological characteristics. This is the first report of shoot blight caused by P. citricola Sawada in lilac suckers in Korea.

• The Plant Pathology Journal
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• 제27권1호
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• pp.78-84
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• 2011

Antagonistic rhizobacteria, more specifically fluorescent pseudomonads and certain species of Bacillus, are known as biocontrol agents of fungal root diseases of agronomic crops. In this study, 144 bacteria were isolated from cucumber rhizosphere and screened as potential biological control agents against Phytophthora drechsleri, the causal agent of cucumber root rot, in vitro condition. Non-volatile compounds of 23 isolates showed noticeable inhibition zone (> 30%) against P. drechsleri, whereas volatile compounds of 7 isolates could prevent more than 30% of the mycelial growth of the fungus. All promising isolates, except of Pseudomonas flourescens V69, promoted significantly plant growth under in vitro condition. P. flourescens CV69 and V11 exhibited the highest colonization on the root. Results of the greenhouse studies showed that a reduction in disease incidence by use of some strains, and particularly use of strains CV6 and V11 as a soil treatment, exhibited a reduction in disease incidence so that suppressed disease by 85.71 and 69.39% respectively. Pseudomonas flourescens CV6 significantly suppressed disease in comparison to Ridomil fungicide. The use of mixture bacterial strains in the soil inoculated by the fungus resulting in falling down the most of the plants which didn't show significant difference with infected control soils without bacteria.

• The Plant Pathology Journal
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• 제21권3호
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• pp.293-296
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• 2005

A leaf blight caused by a species of Phytophthora was found on fatsia plants (Fatsia japonica Decne et Planch.) growing in an apartment garden in Daegu, Korea in late April to May, 2003. The species of Phytophthora isolated from the diseased plants produced sporangia and sex organs on V8 juice agar medium. Sporangia were papillate, ovoid to subspherical, and caducous with a pedicel. The dimensions of the sporangia were $31.2-46.8\times23.4-33.2{\mu}m$ in range, $39.6\pm4.1\times28.3\pm2.8{\mu}m$ in $average{\pm}standard$ deviation, I/b ratio approximately 1.40, with papillae about $3.6{\mu}m$ high, and pedicels $0.9­5.8{\mu}m$ long. Oogonia were spherical, $25.0-32.5{\mu}m$ in range with an average of $28.2\pm2.3{\mu}m$ in diameter. Antheridia were predominantly paragynous, globose to ovoid, $8.8-13.8\times7.5-10.0{\mu}m$ with an average of $10.9\pm1.2 \times9.2\pm1.1{\mu}m$. Oospores in the oogonia were aplerotic or plerotic, and $20.0-25.0{\mu}m$ in diameter with an average of $23.5\pm1.5{\mu}m$. Pathogenicity of the isolate was confirmed on detached leaves of fatsia. The disease was observed only in April and May of 2003 when the weather was unusually wet. It then diminished with increase of temperature in the year and did not appear again on the same plants in 2004. Thus, the fungus appeared to be a relatively weak pathogen of fatsia.

• 식물병연구
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• 제18권2호
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• pp.73-79
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• 2012

Phytophthora katsurae는 밤나무 잉크병의 원인이 되는 병원성 균류이다. P. katsurae를 검출하기 위하여 2개의 duplex PCR primer set을 제작하였다(SOPC 1F/1R+KatI 3F/5R, SOPC 1-1F/1-1R+KatI 3F/5R). SOPC 1F/1R과 SOPC 1-1F/1-1R primer pair는 sequence characteristic amplification regions(SCAR)를 이용하여 제작하였고, KatI 3F/5R primer pair는 internal transcribed spacer(ITS) 부위로부터 제작하였다. 추출한 genomic DNA를 1 mg/ml에서 1 ng/ml까지 10배씩 순차적으로 희석하여 균사량에 따른 Duplex PCR의 민감도를 확인한 결과, 2개의 primer set은 각각 1 ${\mu}g/ml$와 100 ng/ml까지 검출이 가능하였다. 유주포자를 $1{\times}10^6$부터 $1{\times}10^2$ cells/ml까지 10배씩 순차적으로 희석하고 genomic DNA를 추출하여 P. katsurae의 유주포자량에 의한 검출 한계를 확인한 결과, 2개의 primer set 모두 $1{\times}10^5$ cells/ml까지 검출할 수 있었다. 각각의 primer set은 PCR 검출을 실시하였을 때 P. katsurae 균주에서만 PCR 산물이 증폭된 반면에, Phytophthora 16종에서는 P. katsurae에 특이적인 PCR 증폭산물이 생성되지 않았다. 따라서, 2개의 primer set을 이용한 Duplex PCR은 P. katsurae의 신속하고 효과적인 검출에 유용하게 활용될 수 있을 것이다.

• Applied Biological Chemistry
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• 제41권8호
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• pp.600-604
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• 1998

Methanol extracts from 53 species of oriental medicinal plants in 34 families were tested for their fungicidal activities against Pyricularia grisea, Rhizoctonia solani, Phytophthora capsici, Phytophthora infestans, Collectotrichum dematium, Botryospaeria dothidea, Fusarium oxysporum f. sp. cucumerinum, Botrytis cinerea, Puccinia recondita, and Erysiphe graminis. In in vitro study using impregnated paper disc method, the efficacy varied with both plant pathogen and plant species tested. Methanol extracts of Asarum sieboldii roots, Sinomenium acutum roots, Pinus densiflora leaves, Rheum undulatum root barks, Coptis japonica roots, and Phellodendron amurense barks showed potent fungicidal activities against the various pathogens when treated with 10 mg/disc. In a whole plant test, methanol extracts of P. densiflora leaves and roots and C. japonica roots were highly effective against a variety of plant pathogens. As a naturally occurring fungicide, P. densiflora- and C. japonica-derived materials could be useful as new fungicidal products against various plant diseases induced by plant pathogenic fungi.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.128.1-128
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• 2003

A leaf blight disease was found on Fatsia japonica plants growing in the first author's apartment garden in May, 2003. Major symptoms were leaf blight and petiole rot. A species of Phytophthora was isolated from the lesions. The isolate readily produced sporangia and sex organs on V8 juice agar plates. Sporangia were papillate, ovoid to subspherical and caducous with a pedicel. Sporangia were 33.6-38.4 ${\times}$ 33.6$\mu\textrm{m}$ with 1/b ratio approximately 1.14, papillae 4-5$\mu\textrm{m}$ high, pedicels also 4-5$\mu\textrm{m}$ long. Oogonia were spherical, 28.8$\mu\textrm{m}$ in diameter. Antheridia were globose, 14.4$\mu\textrm{m}$ in diameter and mating with oogonia paragynously. Mycelia grew best at 30$^{\circ}C$ and did not grow at 35$^{\circ}C$ or above, and at 5$^{\circ}C$. The morphological characteristics conformed to P. cactorum (Leb. And Cohn) Schroeter.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.128.2-128
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• 2003

Shoot blight was occurring on shoots of suckers of common lilac (Syringa vulgaris L.) growing in first author's apartment garden in May 2003. A species of Phytophthora was isolated from the lesions. The isolate did not sporulate on agar media but formed sporangia in water and also formed sex organs in single culture. Sporangia were semipapillate, ovoid obpyriform, measured 45.6-52.8 ${\times}$ 33.6-36.0$\mu\textrm{m}$. Sporagia were very variable in shape. Optimum temperature for mycelial growth was 25$^{\circ}C$. Oogonia were spherical and antheridia were paragynous. Optimum temp for mycelial growth was 25$^{\circ}C$. The isolate was identified as Phytopkhora citricola on the basis of the morphological characteristics and cardinal temperature.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.127.3-128
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• 2003

A leaf blight disease was found on peony plants growing in the first author's apartment garden in May, 2003. A species of Phytophthora was isolated from the lesions. The isolate readily produced sporangia and sex organs on V8 juice agar plates. Sporangia were papillate, ovoid to subspherical and caduceus with a pedicel. Sporangia were 33.6-38.4 ${\times}$ 33.6$\mu\textrm{m}$ with 1/b ratio approximately 1.14, papillae 4-5$\mu\textrm{m}$ high, pedicels also 4-5$\mu\textrm{m}$ long. Oogonia were spherical, 28.8$\mu\textrm{m}$ in diameter. Antheridia were globose, 14.4$\mu\textrm{m}$ in diameter and mating with oogonia paragynously. Mycelia grew best at 30$^{\circ}C$ and did not grow at 35$^{\circ}C$ or above, and at 5$^{\circ}C$. The morphological characteristics conformed to P. cactorum.