• Title/Summary/Keyword: Phytophthora capsici

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Effect of Acidic Electrolyte Water on Growth and Infection of Phytophthora capsici (고추 역병균(Phytophthora capsici)의 발육과 감염에 미치는 산성전해수의 영향)

  • 이중환;권태룡;문재덕;이준탁
    • Korean Journal Plant Pathology
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    • v.14 no.5
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    • pp.440-444
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    • 1998
  • This experiment was carried out to elucidate the effect of electrolytic water on the growth and infection of Phytophthora capsici. Zoospores of P. capsici did not grow on potato dextrose agar when the pathogen was cultured after suspended in electrolytifc water (pH 2.5, 3.0, 3,5) with HCI solution. When the 100 ml of electrolytic water (pH 2.5, 3.0, 3.5) was irrigated on the red pepper plants that had been inoculated by P. capsici (103 zoospores/ml), the red pepper plants were not infected but irrigated with sterilized water (pH 6.5) the red pepper plants were infected. With this result, it could be concluded that the good sterilization effect on P. capsici might be obtained by applying electrolytic water.

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Identification and Antifungal Antagonism of Chryseomomas luteola 5042 against Phytophthora capsici (고추역병균 Phytophthora capsici의 생육을 저해하는 Chryseomonas luteola 5042의 선발과 항진균성 길항작용)

  • 윤경현;이은탁;김상달
    • Microbiology and Biotechnology Letters
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    • v.29 no.3
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    • pp.186-193
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    • 2001
  • A powerful antagonistic bacterium against Phytophthora capsici causing phytophthora blight of red pepper was isolated from the cultivated soil in Kyongju Korea, The bilogical control mechanisms of the isolated strain were caused by strong antifungal antibiotic, siderophore and cellulase. The strain was identified as Chryseomonas luteola by the cultural morphological and physiological characteristics. The opti- mal culture medium for the antibiotic production was determined as follows : 0.15%D(+) cellobiose, 0.55% $NH_4$CI, 0.01% KCI 0.7% $K_2$$HPO_4$ 0.2% $KH_2$PO$_4$ and 0.5% sodium citrate at pH 7.0 The optimal incubation time was 84 hours at $30^{\circ}C$ In pot bioassay, the treatment of C luteola 5042 protected red pepper plant against the blight of Phytophthora capsici.

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Selection and Antagonistic Mechanism of Pseudomonas fluorescens 4059 Against Phytophthora Blight Disease (고추역병과 시들음병을 방제하는 토착길항세균 Pseudomonas fluorescens 4059의 선발과 길항기작)

  • Jeong, Hui-Gyeong;Kim, Sang-Dal
    • Microbiology and Biotechnology Letters
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    • v.32 no.4
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    • pp.312-316
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    • 2004
  • In oder to select the powerful rhizophere-dorminatable biocontrol agent, we had isolated an indigenous antagonistic bacterium which produced antibiotic and siderophore from a disease suppressive local field soil of Gyungsan, Korea. And we could select the Pseudomosp. 4059 which can strongly antagonize against Fusarium oxysporum and Phytophthora capsici by two kinds of antifungal mechanism that can be caused by the antibiotic of Phenazin, a siderophore and a auxin like subThe selected strain was identified as Pseudomonas fluorescens (biotype A) 4059 by biochemical tests, API $\textregistered$ test, MicroLog TM system and 16S rDNA analysis. The selected antagonistic microorganism, Pseudomosp. 4059 had an antifungal mechanism of antifungal antibiotic and sidrophore. And we were confirmed the antagonistic activity of P fluorescens 4059 with in vitro antifungal test against Phytophthora capsici and in vivo by red-pepper.

A Study on the Inactivation of Phytophthora Blight Pathogen (Phytophthora capsici) using Plasma Process (플라즈마 공정을 이용한 고추역병균(Phytophthora capsici) 불활성화에 관한 연구)

  • Kim, Dong-Seog;Park, Young-Seek
    • Journal of Environmental Science International
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    • v.23 no.9
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    • pp.1601-1608
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    • 2014
  • Plasma reactor was used for the inactivation of Phytophthora capsici which is phytophthora blight pathogen in aquiculture. Effects of first voltage, second voltage, air flow rate, pH, incubation water concentration were examined. At the low $1^{st}$ voltage, under 80 V, the lag phase was noticed within 30 sec, however, it was not shown over 100 V. The variation of optimum operation condition was not shown by the variation of microorganisms. However, the inactivation rate was different by the variation of species of microorganisms. The inactivation rate and efficiency were increased by the increase of $2^{nd}$ voltage. The highest initial inactivation rate was shown at pH 3 and the rate was decreased by the increase of pH. The inactivation rate increased by the increase of air flow rate, however, it was shown as similar at the rate of 4 L/min and 5 L/min. The inactivation rate was distinctly decreased at the three times concentration of incubation solution comparing at the distilled water and basic incubation solution.

A Light and Electron Microscopical Study of Compatible and Incompatible Interactions between Phytophthora capsici and Tomato (Lycopersicon esculentum) (Phytophthora capsici 균주와 토마토의 친화적, 불친화적 상호작용에 대한 광학 및 전자현미경적 연구)

  • 황재순;황병국;김우갑
    • Korean Journal Plant Pathology
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    • v.10 no.2
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    • pp.83-91
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    • 1994
  • Stem tissues of tomato plants (cv. Kwanyang) inoculated with Phytophthora capsici were examined by light and electron microscopy to compare early cytological differences between comaptible and incompatible interactions of tomatoes with the fungus. Twenty four hours after inoculation, the compatible isolate S 197 colonized severely the epidermis, cortex, and xylem vessels of stem tissue, whereas only few fungal cells colonized the stem tissues inoculated with the incompatible isolate CBS 178.26. Fragmented plasma membrane, distorted chloroplast, degraded cell wall, remnants of host cytoplasm were early ultrastructural features of the damaged host cell observed both in the compatible and incompatible interaction, a number of vesicles were distributed in the space between fungal cell walls and plasma membrane. The degradation of host cell walls by P. capsici was more pronounced in the compatible than the incompatible interactions. The incompatible interactions of tomato cells with P. capsici were characterized by formation of host cell wall apposition in the cortical parenchyma cells, indicating that the apposition of electron-dense material from the host cell walls may function as a plant defense reaction to the fungus. The fungal cells encased by wall appositions had abnormal cytoplasm and separated plasma membranes. The haustorium which formed from the fungal hyphae did not further penetrate through the host wall apposition and cytoplasmic aggregation, especially in the incompatible reactions. In contrast, the haustorium of the compatible isolate S 197 was not encased by wall appositions.

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Tn5 lac Mediated Mutagenesis of Enterobacter sp. B54 Antagonistic to Phytophthora capsici. (Phytophthora capsici의 성장을 저해하는 Enterobacter sp. B54의 선발과 Tn5 lac을 이용한 돌연변이 유기)

  • Yoon, Sang-Hong;Choi, Chung
    • Microbiology and Biotechnology Letters
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    • v.26 no.5
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    • pp.393-399
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    • 1998
  • Enterobacter sp.B54 which shows antagonistic activity to Phytophthora capsici on potato dextrose agar was selected among 112 strains isolated from Korean soil. After Tn5 lac-induced mutants were obtained through Pl :: Tn5 lac mutagenesis, 2 mutants for loss of antibiosis and 1 mutant for increased antibiosis were screened by using in vitro fungal inhibition assay. When the 3 mutants affected in antibiosis were analyzed by southern hybridization with pRZ102 (ColEl :: Tn5) as a probe, its results suggest that Tn5 lac was randomly inserted into different chromosomal sites in these mutants.

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Mating Types of Phytophthora capsici Leonian from Red-pepper ( Capsicum annuum L.) in Korea (고추역병균(疫病菌)(Phytophthora capsici Leonian)의 배우자형(配偶子型) 분포(分布))

  • Kim, Jeong-Soo;Do, Tae-Hong;Cho, Eui-Kyoo;Lee, Min-Woong
    • The Korean Journal of Mycology
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    • v.16 no.2
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    • pp.60-63
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    • 1988
  • Each of 103 isolates of Phytophthora capsici was obtained from diseased red pepper plants randomly belonged to either the mating type $A_1$ or the mating type $A_2$. Fifty four isolates were classified as mating type $A_1$, and 49 isolates were classified as mating type $A_2$.Oospores were formed in each combination of isolates between $A_1$ or $A_2$ on 5% V-8 juice agar except one combination.

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Development of Repetitive DNA Probes for Genetic Analysis of Phytophthora capsici (Phytophthora capsici의 유전적 특성 분석을 위한 Repetitive DNA Probe의 개발)

  • Song, Jeong-Young;Kim, Hong-Gi
    • The Korean Journal of Mycology
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    • v.30 no.1
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    • pp.66-72
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    • 2002
  • To develop DNA markers for analysis of genetic characteristics of Phytophthora capsici population, randomly selected clones from HindIII-digested genomic DNA library of P. capsici 95CY3119 were surveyed by hybridizing to Southern blots of HindIII-digested total genomic DNA of P. capsici. Probe DNAs inserted into selected individual clones strongly hybridized with HindIII digests of P. capsici. Among probes examined, PC9 revealed the repetitive and highly polymorphic bands to HindIII digests of inter-and intra-field P. capsici isolates. Genetic diversity of individual isolates was also clearly revealed in cluster analysis based on its band patterns. The other probe, PC22, was hybridized only to DNA from P. capsici and this was highly repetitive. However, there was no response to other Phytophthora species and Pythium sp. These DNA probes could be used as very useful markers in analysing genetic diversity and identification for P. capsici population throughout the world.

Protoplast Formation and Regeneration from Mycelia of Phytophthora capsici (Phytophthora capsici의 균사체(菌絲體)로부터 원형질체(原形質體) 형성(形成)과 재생(再生))

  • Yi, Seung-Youn;Kim, Young-Jin;Hwang, Byung-Kook
    • The Korean Journal of Mycology
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    • v.21 no.1
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    • pp.1-8
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    • 1993
  • ABSTRACT: Factors responsible for protoplast formation and regeneration of Phytophthora capsici were examined. Protoplasts were successfully liberated from the mycelial culture by digestion for 6-9 hrs with Novozym 234 in 0.35 M $CaCl_2$, (pH 5.7) as osmotic stabilizer. Young rapidly-growing mycelium (24 hrs old) showed highest protoplast yields. High concentrations of Novozym 234 were effective in releasing protoplasts from the mycelium. The combination of 0.4 M mannitol and 0.1 M $CaCl_2$ was optimal osmotic stabilizers for protoplast regeneration. The synthetic Henninger media containing all nutritional elements gave the best regeneration rate. The protoplast regeneration was greatly inhibited in the media which were not supplement with amino acids or ${\beta}-sitosterol$. Certain amino acids such as L-aspartic acid and L-glutamic acid remarkably enhanced protoplast regeneration. However, the addition of microelements did not affect protoplast regeneration.

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Concanamycin B, Active substance Against Phytophthora capsici Produced by Streptomyces neyagawaensis 38D10 Strain (Streptomyces neyagawaensis 38D10 균주가 생산하는 concanamycin B의 항고추역병 활성)

  • Kim, Chang-Jin;Lee, In-Kyoung;Yun, Bong-Sik;Yoo, Ick-Dong
    • Microbiology and Biotechnology Letters
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    • v.21 no.4
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    • pp.322-328
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    • 1993
  • During the screening of antifungal compounds from microbial secondary metabolites to control phytophthora blight of red pepper caused by Phytophthora capsici, a soil isolate, strain 38D10 was selected. Based on taxonomic studies, this strain was identified as Streptomyces neyagawaensis. The antifungal compound was purified from culture broth by HP-20 column chromatography, ethyl acetate extraction, silica gel column chromatography, HPLC and identified as concanamycin B by UV. $^1H$-NMR, $^{13}C$-NMR, SIMS analysis. Concanamycin B has strong antifungal activity against some phytopathogenic fungi but not antivacterial activity and preventive value were 50% and 100% at 125ppm and 250ppm in pot assay.

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