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Physiological Characteristics and Immunomodulating Activity of Streptococcus macedonicus LC743 Isolated from Raw Milk

  • Cho, Seong-A;Kim, Kee-Sung;Do, Jeong-Ryong;Kim, Sae-Hun;Lim, Sang-Dong
    • 한국축산식품학회지
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    • 제30권6호
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    • pp.957-965
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    • 2010
  • To develop a new starter culture for fermented milk, Streptococcus macedonicus LC743 was isolated from raw milk and its physiological characteristics were investigated. S. macedonicus LC743 showed good immunomodulating activity compared to the index LAB starters tested. The optimum growth temperature of S. macedonicus LC743 was $40^{\circ}C$, and it took 18 h to reach pH 4.34 under these conditions. S. macedonicus LC743 showed higher sensitivity to novobiocin in a comparison of 15 different antibiotics and showed the highest resistance to gentamycin. It also showed higher activities of leucine arylamidase and acid phosphatase. Moreover, it was comparatively tolerant to bile juice and acid and displayed high resistance to Escherichia coli, Salmonella Typhimurium, Staphylococcus aureus with rates of 80.0%, 68.42%, and 81.54% respectively. These results demonstrate that S. macedonicus LC743 could be an excellent starter culture for fermented milk with a high level of immunomodulating activity.

Morphological, Physiological and Biochemical Responses of Gerbera Cultivars to Heat Stress

  • Chen, Wen;Zhu, Xiaoyun;Han, Weiqing;Wu, Zheng;Lai, Qixian
    • 원예과학기술지
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    • 제34권1호
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    • pp.1-14
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    • 2016
  • Heat stress is an agricultural problem for Gerbera jamesonii, and it often causes poor seedling growth, reduced flower yield and undesirable ornamental characteristics of flowers. However, little is known about the effect of heat stress on the morphological, physiological and biochemical characteristics of gerbera plants. Here, the responses of six cultivars of Gerbera jamesonii to heat stress were investigated. Under a 1-d heat treatment at $45^{\circ}C$, the leaves of gerbera cultivars showed yellowing, wilting, drying and death to varying degrees. The heat treatment also resulted in increased electrical conductivity, decreased soluble protein and chlorophyll contents, and the accumulation of malondialdehyde (MDA) and proline in leaves. Moreover, heat tolerance differed among the six tested gerbera cultivars. Our results demonstrated that among the six gerbera cultivars, 'Meihongheixin' is a heat-resistant cultivar, whereas 'Beijixing' is a heat-sensitive one. 'Shijihong' and 'Linglong' are relatively heat-resistant cultivars, and 'Dadifen' and 'Taiyangfengbao' are relatively heat sensitive.

Physiological Characteristics and Immunomodulating Activity by Lactobacillus paracasei subsp. paracasei BFI46 Isolated from New-Born Infant Feces

  • Lim, Sang-Dong;Kim, Kee-Sung;Cho, Seong-A;Do, Jeong-Ryong
    • 한국축산식품학회지
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    • 제30권2호
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    • pp.223-231
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    • 2010
  • In order to develop a new starter for fermented milk, Lactobacillus paracasei subsp. paracasei BFI46 (BFI46) obtained from new-born infant feces was investigated for physiological characteristics. Good immunomodulating activity was evident compared with commercial lactic acid bacteria starter cultures. The optimum growth temperature of BFI46 was $40^{\circ}C$ with 12 h required to reach pH 4.3. Testing with 13 different antibiotics revealed greatest sensitivity of BFI46 to penicillin- G and chloramphenicol, and heightened resistance to neomycin, kanamycin and polymyxin. BFI46 displayed higher esterase activities compared to 18 other enzymes, was comparatively tolerant to bile juice and able to survive at pH 2 for 3 h, and displayed high resistance against Escherichia coli and Salmonella Typhimurium with a survival rate of 57.14% and 96.36%, respectively. The results indicate that BFI46 could be an excellent starter culture for fermented milk with high level of immunomodulating activity.

Lactobacillus plantarum KI134의 생리적 특성 및 이 균에 의한 우유 발효물의 항비만효과 (Physiological Characteristics and Anti-Obesity Effect of Milk Fermented by Lactobacillus plantarum KI134)

  • 김슬기;임상동
    • Journal of Dairy Science and Biotechnology
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    • 제38권4호
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    • pp.207-221
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    • 2020
  • This study aimed to investigate the physiological characteristics and anti-obesity effects of milk fermented by L. plantarum KI134. The lipase, α-amylase, and α-glucosidase inhibitory activities of milk fermented by L. plantarum KI134 was 94.57±1.25%, 9.44±2.85%, and 2.74±1.24% (10 fold dilution), respectively. L. plantarum KI134 showed higher sensitivity to clindamycin and erythromycin in comparison to sixteen different antibiotics. It demonstrated the highest resistance toward ampicillin and vancomycin. The strain showed higher β-galactosidase, leucine arylamidase, valine arylamidase, acid phosphatase, β-glucosidase, and N-acetyl-β-glucosaminidase activities compared to other enzymes. It also did not produce carcinogenic enzymes, such as β-glucuronidase. The survival rate of L. plantarum KI134 in 0.3% bile was 96.90%. Moreover, the strain showed a 91.45% survival rate at a pH of 2.0. L. plantarum KI134 has resistance to Escherichia coli, Salmonella Typhimurium, Listeria monocytogenes, and Staphylococcus aureus at the rates of 70.00%, 68.18%, 59.05%, and 40.63%, respectively. L. plantarum KI134 (23.01%) showed higher adhesion ability than the positive control (16.32%) L. rhamnosus GG. These results demonstrated that milk fermented by L. plantarum KI134 demonstrated an anti-obesity effect under in vitro conditions, with confirmed potential as a probiotic.

찰녹미 첨가 발효음료 품질 특성 (Quality Characteristics of Fermented Rice Beverage Prepared with Green Glutinous Rice)

  • 조민정;정희선
    • 한국식품영양학회지
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    • 제36권5호
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    • pp.341-353
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    • 2023
  • In this study, we aimed to develop a fermented green rice beverage with a unique flavor and physiological function activity. With glutinous green rice and rice nuruk as independent variables, we modeled the antioxidant characteristics and α-glucosidase, α-amylase inhibitory activity of glutinous green rice fermented beverage to verify its significance. The total flavonoid content and α-glucosidase inhibitory activity were selected as Quadratic models, and DPPH radical scavenging ability and α-amylase inhibitory activity were selected as linear models. For the sensory characteristics of glutinous green rice fermented beverage, sweetness, sourness, savory taste, bitterness, throat feel, nuruk scent, and overall preference increased in preference as the amount of glutinous green rice and rice nuruk increased, but significantly decreased after the center point (p<0.01). A blending ratio of 180.00 g of glutinous green rice and 400.00 g of rice nuruk had the highest preference among all the sensory items. Based on these results, we developed a green rice fermented beverage with unique flavor and physiological function activity of rice using glutinous green rice and rice nuruk, and the optimal blending ratio was determined to be 164.04 g of white rice, 195.96 g of glutinous green rice, and 414.61 g of rice nuruk.

Evaluation of likes and Dislikes during Visual Stimuli by Electroence phalography

  • Suo, Y.;Marusei, O.;Takeda, A.;Watanuki, S.
    • 한국감성과학회:학술대회논문집
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    • 한국감성과학회 2000년도 춘계 학술대회 및 국제 감성공학 심포지움 논문집 Proceeding of the 2000 Spring Conference of KOSES and International Sensibility Ergonomics Symposium
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    • pp.58-61
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    • 2000
  • The purpose of this study is to investigate the characteristics of electroencephalogram (EEG) during emotional occurrence of likes and dislikes in humans subjected to visual stimuli. Fifteen healthy male subjects participated in the study. Portrayals of females and cars on a visual screen, one photographic display at a time. were used as the stimulus. The subjects, with their EEGs concomitantly monitored, were asked to record their likes and dislikes for each portrayal of a total of 50 sequential displays. The results indicated that beta ($\beta$)=wave was more prevalent with dislikes than likes, and the arousal level was higher when dislikes predominated over likes, implying that more cerebral information processing activity was involved in answering dislikes than likes. In the case of cars, the difference between likes and dislikes was focused within a frequency band of 15-20 Hz in the right cerebral hemisphere. Our findings suggest that the activity in the right brain predominates with increases in negative emotion.

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Fluid-structure interactions of physiological flow in stenosed artery

  • Buriev, Bahtiyor;Kim, Tae-Dong;Seo, Tae-Won
    • Korea-Australia Rheology Journal
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    • 제21권1호
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    • pp.39-46
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    • 2009
  • Atherosclerosis is a disease that narrows, thickens, hardens, and restructures a blood vessel due to substantial plaque deposit. The geometric models of the considered stenotic blood flow are three different types of constriction of cross-sectional area of blood vessel; 25%, 50%, and 75% of constriction. The computational model with the fluid-structure interaction is introduced to investigate the wall shear stresses, blood flow field and recirculation zone in the stenotic vessels. The velocity profile in a compliant stenotic artery with various constrictions is subjected to prescribed physiologic waveform. The computational simulations were performed, in which the physiological flow through a compliant axisymmetric stenotic blood vessel was solved using commercial software ADINA 8.4 developed by finite element method. We demonstrated comparisons of the wall shear stress with or without the fluid-structure interaction and their velocity profiles under the physiological flow condition in the compliant stenotic artery. The present results enhance our understanding of the hemodynamic characteristics in a compliant stenotic artery.

Physiological Characteristics and GABA Production of Lactobacillus plantarum K255 Isolated from Kimchi

  • Park, Sun-Young;Kim, Kee-Sung;Lee, Myung-Ki;Lim, Sang-Dong
    • 한국축산식품학회지
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    • 제33권5호
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    • pp.595-602
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    • 2013
  • As a major inhibitory neurotransmitter of the central nervous system in animals, ${\gamma}$-aminobutyric acid (GABA) has several physiological functions, such as anti-hypertensive, diuretic, tranquilizer and anti-stress effects in human. In order to determine strains with high GABA producing ability and glutamate decarboxylase (GAD) activity, 273 bacteria were isolated from various types of Kimchi. Strain K255 contained $386.37{\mu}g/mL$ of GABA in MRS broth containing 1% MSG, $600.63{\mu}g/mL$ of GABA in MRS broth containing 2% MSG and $821.24{\mu}g/mL$ of GABA in MRS broth containing 3% MSG. It showed that K255 had the highest GABA production ability compared to other commercial lactic acid bacteria. K255 was identified as Lactobacillus plantarum based on its API carbohydrate fermentation pattern and 16S rDNA sequence. K255 was investigated for its physiological characteristics. The optimum growth temperature of K255 was $37^{\circ}C$and cultures took 13 h to reach the pH 4.4. K255 showed more sensitive to bacitracin in a comparison of fifteen different antibiotics, and showed most resistance to kanamycin and vancomycin. Moreover, it was comparatively tolerant to bile juice and acid and displayed resistance to Escherichia coli, Salmonella Typhimurium, Staphylococcus aureus, with rates of 30.8%, 29.7%, and 23.4% respectively. These results demonstrate that K255 could be an excellent strain for the production of functional products.

관수주기와 상토조성이 자생 골고사리(Asplenium scolopendrium)의 생육과 생리에 미치는 영향 (Effects of Irrigation Times and Soil Media on the Growth and Physiological Characteristics of Native Fern Asplenium scolopendrium)

  • 주진희;방광자
    • 한국조경학회지
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    • 제32권6호
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    • pp.109-116
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    • 2005
  • This study was conducted to examine the growth and physiological characteristics of Asplenium scolopendrium native fern as affected by irrigation times and soil media as an environment modeled on habitate where was sunken-condition. 1. Light intensity was lower in sunken than in non-sunken, but air humidity was higher in sunken about $2040\%$. Soil moisture content was higher with the leaf mold in sunken irrigating 2 times/week. The results of chemical analysis of medium showed that EC, pH, organic matter content, total nitrogen, CEC, Exch-Ca, Exch-Mg and Exch-K were higher with leaf mold than sud: leafmold and field soil: sud: leaf mold. 2. In the case of irrigation 2 times/week Asplenium scolopendrium grew well sunken more than non-sunken. As non-sunken condition similar with, 7 times/week irrigation, plant height, frond width, frond length and stipe length increased. In case of soil media, growth of Asplenium scolopendrium was better with leaf mold than that of sand: leafmold or field soil: sand: leaf mold. 3. In the case of irrigation 2 times/week photosynthetic rate, $CO_2$ absorption rate and water efficiency were higher with non-sunken than that of sunken, expect of stomatal conduction, $CO_2$ use efficiency. The physiological characteristics of Asplenium scolopendrium were highest in non-sunken irrigating 7 times/week In case of soil media, physiological activity was higher with leaf mold than sand: leafmold or field soil: sand: leaf mold.

혈액투석환자의 피로와 관련 요인에 관한 연구 (Fatigue and its Related Factors in Patients on Hemodialysis)

  • 김혜령
    • 대한간호학회지
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    • 제26권1호
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    • pp.53-72
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    • 1996
  • The Purpose of this study was to identify characteristics of fatigue and the relationship between fatigue and related factors in patients on hemodialysis. This study was a survey study using a cross-sectional design. The subjects for this study were 101 patients on hemodialysis who were registered in the six hemodialysis clinics among a total of eleven clinics in Seoul. The period of data collection was from February 28, 1995 to May 2, 1995. Data were collected through an interview with a structured packet and the physiological data. The tools used in this study were the Visual Analogue Scale-Fatigue developed by Lee et al(1990) and translated by Lee(1991), the fatigue interview schedule developed by this researcher, Zung's self rating depression scale(Zung, 1965), the self-efficacy scale developed by Sherer et al(1982) and the Norbeck Social Support Questionnaire(NSSQ) translated by Oh(1984). The collected data were analyzed using descriptive statistics(mean, standard deviation, frequency, range), Pearson correlation coefficients and Stepwise multiple regression. The results were as follows ; 1. Characteristics of Fatigue of hemodialysis patients : 1) 79 of 101 hemodialysis patients complained fatigue. 2) The mean fatigue score as measured by the VAS-F was 36.2mm. 3) The mean duration of fatigue was 2.9 hours 2. Characteristics of fatigue related factors : 1) The physiologic factor which included Hgb, Hct, BUN, creatinine, potassium and interdialytic weight gain deviated from normal range. 2) The psychological factor which included depression and self-efficacy was about the same level as for patients with other chronic diseases. 3) The environmental factor which included social support had wide variation. 3. The relationship between fatigue and related factors : 1) Interdialytic weight gain in the physiologica factor was the only valuable with fatigue (p<.05) 2) The relationship between fatigue and the psychological factor of depression showed a positive and strong correlation(p<.05). According to the findings of this study, fatigue was highly correlated with the depression. This indicates that nurses should try to assess and control psychological factors when patients complain of fatigue rather than just considering physiological factors. Nursing has to develop effective nursing interventions to reduce fatigue in patients with chronic diseases using the relationship between fatigue and physiological, psychological and environmental factors.

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