• Journal of Microbiology and Biotechnology
• /
• v.8 no.5
• /
• pp.523-530
• /
• 1998

Rapid and quantitative analysis of physiological change and clavulanic acid production was studied by the combination of pyrolysis mass spectrometry (PyMS) and artificial neural network (ANN) in Streptomyces clavuligerus. Firstly, the continuous culture studies were carried out to get the physiological background and PyMS samples. Clavulanic acid production was inversely related to growth rate: Mycelium growth and $q_{cal}$ were optimum at 0.1 $h^{-1}\; and \;0.025 h^{-1}$ respectively. Changes in specific nutrient uptake rates ($q_{gly}$ and $q_{amn}$) also affected clavulanic acid production since clavulanic acid production appeared to be stimulated by the limitation of carbon and nitrogen. Fermentation broth containing mycelium taken from continuous cultures was analyzed by PyMS, and the PyMS spectra were analyzed with multivariate statistics. PCCV plots revealed that samples harvested under the same culture condition were clustered together but samples from different culture conditions formed separate clusters. To deconvolute the pyrolysis mass spectra so as to obtain quantitative information on the concentration of clavulanic acid, ANN was trained on Py MS data using a radial basis function classifier. The results showed that the physiological stages with different growth rate were successfully differentiated and it was possible to monitor the clavulanic acid production precisely and rapidly.

• Journal of Korean Biological Nursing Science
• /
• v.5 no.2
• /
• pp.41-51
• /
• 2003

Purpose : This descriptive study was performed to analyze the trends of nursing interventional studies in adult nursing area. Method : All interventional studies of 'Journal of Korean Academy of Adult Nursing Society', fifty three articles, from 1989 to 2000 were collected and reviewed Result : The research subject were patients in 38(66.7%) and 81.8% of researches was performed in hospital environment. There was only one true experimental design and most of articles(79.2%) were designed by quasi-experimental method Only 17% of articles have theoretical framework and 73.6% of articles described research hypotheses. The data collection methods for variables were as follows: Psychosocial measurement 60.8%, physiological measurement 25.7%, and observation 13.5%, respectively. Lecture(16.7%), audio-visual(16.7%), and book, pamphlet, or leaflets(13.6%) were the most frequently used instruments of the interventions. Behavioral domain(60.3%) and Physiological: Basic domain(29.3%) was the most common, when the interventions were classified by Nursing Intervention Classification. Physiological(50.8%) and psychosocial variables(49.2%) were the two major dependent variables. Conclusion : Considering the above results obtained, the nursing intervention studies in adult nursing area have been tested with quasi-experimental studies through clinical trials. For the development of clinical nursing practice and advancement of nursing theories, the amount of interventional studies should be increased and the quality of research methods refined more to develop nursing science and practice in adult nursing area.

• Asian Pacific Journal of Cancer Prevention
• /
• v.15 no.13
• /
• pp.5299-5303
• /
• 2014

Background: To explore etiology for providing scientific clues for the prevention of lung cancer. Materials and Methods: Data for lung cancer incidence and meteorological geographic factors from 25 counties in Zhejiang province of China during 2011 were studied. Stepwise multiple regression and correlation analysis were performed to analyze the geographic distribution and epidemiology of lung cancer. Results: 8,291 new cases (5,998 in males and 2,293 females) of lung cancer during 2011 in Zhejiang province were reported in the 25 studied counties. Reported and standardized incidence rates for lung cancer were 58.0 and 47.0 per 100,000 population, respectively. The incidence of lung cancer increased with age. Geographic distribution analysis shows that the standardized incidence rates of lung cancer in northeastern Zhejiang province were higher than in the southwestern part, such as in Nanhu, Fuyang, Wuxing and Yuyao counties, where the rates were more than 50 per 100,000 population. In the southwestern Zhejiang province, for instance, in Yueqing, Xianju and Jiande counties, the standardized incidence rates of lung cancer were lower than 37 per 100,000 population. Spearman correlation tests showed that forest coverage rate, air quality index (AQI), and annual precipitation level are associated with the incidence of lung cancer. Conclusions: Lung cancer in Zhejiang province shows obvious regional differences. High incidence appears associated with low forest coverage rate, poor air quality and low annual precipitation. Therefore, increasing the forest coverage rate and controlling air pollution may play an important role in lung cancer prevention.

• Korean Journal of Oriental Medicine
• /
• v.2 no.1
• /
• pp.84-103
• /
• 1996

The physiological signals measured by Oriental Medicine instruments have been analyzed quantitatively in the view of the rule of promoting and counteracting relation of five evolutive phases theory. We tried to reduce the physiological signals measured by EAV(Elec-tro-Acupuncture according to Voll) and IR thermography to the representation of five evelutive phases. The EAV index and local skin temperature on acupuncture points of each phases measured and normalized so that the total value of five phases became unity. We assumed that the normalized EAV index and local skin temperature mean the deficiency or excess of Qi for each phases. The state of Qi distribution for each phases were approximately agree with the diagnostic pattern of O. M. doctor. Taking account of the Qi distribution state of·or the five evolutive phases, we performed a proper needle insertion on acupuncture points to induce the distinct change of Qi for each phases. We compared the measured results with the predictions of Qi variation by the rule of pro- moting and counteracting relation over the five evolutive phases. For all cases, the variation of Qi in the own phase on which a needle insertion was performed were exactly same to the theoretical prediction and partial agreement was shown for the other four phases. The same analysis was carried to the results of skin temperature measurements at accupoints. We found that the local skin temperature at accupoints of each phases shelved a finite change by the needle insertion and the behavior- of its change were strongly correlated to the rule of promoting and counteracting relation of five evolutive phases.

• The Korean Journal of Physiology
• /
• v.3 no.2
• /
• pp.1-7
• /
• 1969

Physiological analysis of swimming in 13 (age:16.3 years, freestyle swimming) and 15 (age:17.2 years, breast stroke swimming) high school boys through oxygen uptake and oxygen debt measurements were performed. The following results were obtained. 1. In freestyle swimming oxygen debt was greater and mechanical efficiency was lower in subjects with less speed. In beginner efficiency was only 1.35%, whereas, in a more skilled subject it ranged to 4.28%. The mean efficiency was 2.59%. 2. In freestyle swimming the speed-oxygen debt curve was convex to the speed axis and the curve shifted to the right the more the speed was greater. 3. Maximal oxygen uptake in breast stroke swimming was 2.51 l/min or 41.8 ml/kin/kg and was 79.3% of treadmill running. Maximal pulmonary ventilation in breast stroke swimming was 73.1 l/min and was 87% of treadmill running. Maximal ventilation equivalent was 2.89 liters. 4. In subjects with greater speed of breast stroke swimming maximal oxygen uptake and mechanical efficiency of swimming were greater. The mechanical efficiency of breast stroke swimming averaged 1.08% $(range:0.51{\sim}1.70%)$. The coefficient of correlation between speed and efficiency was r=.87.

• Science of Emotion and Sensibility
• /
• v.3 no.2
• /
• pp.29-40
• /
• 2000

Autonomic and EEG responses were analyzed in 32 college students exposed to visual stimulation with Korean Affective Picture System (KAPS) and 36 students exposed to the International Affective Picture System (IAPS). Cardiac, electrodermal, and electrocortical measures were recorded during 30 sec of viewing affective pictures. The slides intended to elicit basic emotions (fear, anger, surprise, disgust, and sadness) were presented to subjects via Kodak slide-projector. The aim of the study was to differentiate autonomic and EEG responses associated with the same negative valence emotions elicited by KAPS and IAPS stimulation and to identify the influence of cultural relevance on physiological reactivity. The analysis of obtained results revealed significant differences in physiological responsiveness to emotionally negative valence slides from KAPS and IAPS. The typical response profile for all emotions elicited by the KAPS included HR acceleration (except surprise), and increase of electrodermal activity, slow and fast alpha blocking and fast beta power increase in EEG, which was not associated with significant asymmetry (except fast alpha in sadness). Stimulation with the IAPS evoked HR deceleration, specific electrodermal responses with relatively high tonic electrodermal activation, alpha-blocking and fast beta increase, and was accompanied also by theta power increase and marked frontal asymmetry (e.g., fast beta, theta asymmetries in sadness, fast alpha in fear). Physiological responses to fear and anger-eliciting slides from the IAPS were significantly less profound and were accompanied by autonomic and EEG changes more typical for attention rather than negative affect. Higher cardiovascular and electrodermal reactivity to fear emotion observed in the KAPS, e.g., as compared to data with the IAPS as stimuli, can be explained by cultural relevance and higher effectiveness of the KAPS in producing certain emotions such as fear in Koreans.

• Analytical Science and Technology
• /
• v.8 no.3
• /
• pp.229-236
• /
• 1995

Multiple-step gradient method was used for the analysis of free amino acids in physiological fluids by high-performance liquid chromatography with diode array detector on the Amino Quant $C_{18}$ column under the condition of pH 7.2 of buffer solutions. Plasma samples of normal Korean people and abnormal Korean people who have schizophrenia were subjected to derivatization with o-phthalaldehyde in the presence of 3-mercaptopropionic acid. Quantitative analysis of amino acids in physiological fluids by internal standard method gave highly reproducible results within a relative standard deviation of less than 2~6%. And amino acids amounts of physiological fluids of Korean people gave some different results from those of foreigners. There was large differences in tyrosine amount between normal and abnormal man.

• Journal of the Ergonomics Society of Korea
• /
• v.24 no.1
• /
• pp.9-17
• /
• 2005

81.1% of traffic accidents is attributed to the drivers. In this regard, D/E model is a practical and effective method in terms of the cost and time in evaluating the road hazardousness. To examine the validity of the threshold values by the levels of demand We selected 10 subjects and collected their physiological signals while they were driving on Honam Highway (Jeonju ${\leftrighttarro}$ Hoideog section). Based on the collected data, the hazardous road condition was evaluated using the new threshold values of the effort level determined by cluster analysis. In applying the D/E model, a decision method based on the demand level was suggested, using a traffic accident prediction model. Additionally, the limit value of the effort level was determined using the drivers' physiological signal data collected at the highway. A comparison analysis of the two D/E models revealed no significant difference: The existing method and the clustering method determined 9 and 7 hazardous road zones, respectively, while actual traffic accidents were reported in 6 and 4 zones, respectively among the predicted road hazardous zones. However, the latter method suggested a more scientific and rational basis in determining the limit value of the Effort level. In conclusion, although D/E model has a great merit as a pioneering method to reflect human factors in evaluating the road hazardousness, it is believed that this method could be improved by a more dynamic method that considers the traffic conditions and the individual physiological signal of the drivers simultaneously in determining a better limit.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.19 no.2
• /
• pp.304-314
• /
• 2005

The major concept of Sasang typology is that the disease susceptibility and drug response as well as physiological characteristics are presumed to be different depending on their Sasang types. Although characterizing fundamental basis of their traits are crucial in this research field, only pathological susceptibility and physical appearances were thoroughly studied. We evaluated their physiological characteristics by tapping psychological, physical and genetic traits of each Sasang types. After determining the Sasang type of one hundred three college students based on the Questionnaire for the Sasang Constitution Classification, the psychological, physical and genetic traits of each type were analyzed with the Myers-Briggs Type Indicator (MBTI), Bioelectrical Impedance Analysis and genetic polymorphism test, respectively. Each of the Sasang types showed significantly different profiles (Generalized estimation equation, coef=11.88, z=2.13, p=0.033), and could be distinctively classified based on their MBTI scores (discriminant analysis Wilks Lambda=0.611, df=8, chi-square=36.7, p<0.001). Subjects with the So-Eum type (Introversion and Judging) and the So-Yang type (Extroversion and Perceiving) showed contrasting psychological features, however they had similar anthropometric characteristics. Subjects with the Tae-Eum type showed bigger Body Mass Index ($R^2$=0.22, df=4, 74, F=5.07, p=0.001) and body shape compared to others. Although there were no significant differences in G-protein beta-3 subunit polymorphism, angiotensin-converting enzyme polymorphism and Methylenetetrahydrofolate reductase polymprhisms among groups with Sasang types, it was shown that the dopamine system could be one for genetic marker for Sasang typology. These results demonstrated distinctive and essential traits of Sasang typology using reproducible psychometric, anthropometric and genetic evaluations. We also found that the Sasang typology was a bio-psychological typology which could show trait-specific guideline for individualized medicine.

• Journal of Animal Science and Technology
• /
• v.63 no.1
• /
• pp.125-136
• /
• 2021

Bovine coronavirus (BCoV) is associated with severe diarrhea, dehydration, and depression, which result in significant economic damages in the dairy and beef cattle industries worldwide. However, differences in the gut microbiota structure and their correlations with differing physiological parameters between BCoV-infected calves with diarrhea and recovered calves are not well understood. In this study, fecal specimens were collected from 10 post-weaned calves, before and after 2 months of fluid therapy, and the samples were used for microbiota analysis. Following recovery, the alpha-diversity profiles (observed operational taxonomic units [OTUs], and Chao1, Shannon, and Simpson indices) changed significantly when compared with those of calves with diarrhea. Beta-diversity analysis exhibited significant differences in gut microbiota compositions between calves with diarrhea and those in the recovered state. The abundances of eight phyla and thirteen genera in feces changed markedly after restoration of BCoV diarrhea. In addition, our correlation study clearly revealed that increased abundances of the genera Caproiciproducens, Pseudoflavonifractor, and Oscillibacter negatively correlated with serum glucose, and phosphorus levels, but positively correlated with serum chloride in calves with diarrhea, whereas increased abundances of the genera Peptostreptococcaceae;Clostridium (Clostridium cluster XI), Intestinibacter, Cellulosilyticum, Ruminococcus, Romboutsia, Paeniclostridium, Clostridiaceae;Clostridium and Turicibacter in recovered calves showed the opposite pattern. These results suggest that structural changes of the gut microbiota after recovery from BCoV infection correlate with changes in physiological parameters. In conclusion, our data provide evidence of gut microbiota-composition changes and their correlations with the physical profiles of post-weaned calves, before and after fluid therapy for BCoV-related diarrhea.