• Anatomy and Cell Biology
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• 제56권1호
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• pp.16-24
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• 2023

Endothelial cells (EC) are the anatomical boundaries between the intravascular and extravascular space. Damage to ECs is catastrophic and induces endothelial cell dysfunction. The pathogenesis is multifactorial and involves dysregulation in the signaling pathways, membrane lipids ratio disturbance, cell-cell adhesion disturbance, unfolded protein response, lysosomal and mitochondrial stress, autophagy dysregulation, and oxidative stress. Autophagy is a lysosomal-dependent turnover of intracellular components. Autophagy was recognized early in the pathogenesis of endothelial dysfunction. Autophagy is a remarkable patho (physiological) process in the cell homeostasis regulation including EC. Regulation of autophagy rate is disease-dependent and impaired with aging. Up-regulation of autophagy induces endothelial cell regeneration/differentiation and improves the function of impaired ones. The paper scrutinizes the molecular mechanisms and triggers of EC dysregulation and current perspectives for future therapeutic strategies by autophagy targeting.

• 원예과학기술지
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• 제33권5호
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• pp.625-632
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• 2015

Drought is a severe abiotic stress that affects global crop production. A drought model was created for 'Toyonoka' Fragaria ${\times}$ ananassa, and the effects of drought stress on contents of proline, sugars, and antioxidant enzyme activities were investigated. Strawberry transplants with identical growth were chosen for the experiments and the randomized design included four replications (10 plants per block). The experimental sets differed in the moisture level of the culture medium relative to the range of moisture content as follows: control, 70-85%; mild drought stress, 50-60%; moderate drought stress, 40-50%; and severe drought stress, 30-40%. Drought stress was imposed by limiting irrigation. Plants were sampled and physiological parameters w ere measured on 0, 2, 4, 6, 8, and 10 days after the commencement of droughts tress. The water potential of strawberry leaves decreased in the plants under mild, moderate, and severe stress during the course of the water stress treatment and exhibited a significant difference from the control. Strawberry leaves subjected to drought stress had higher accumulation of proline, sugars, and malondialdehyde, and higher activities of superoxide dismutase, peroxidase, and catalase than leaves of control plants. Malondialdehyde levels increased in parallel with the severity and duration of drought stress. By contrast, antioxidant enzyme activity displayed dynamic responses to drought stress, first increasing and subsequently decreasing as the severity and duration of drought stress increased. These results suggest that strawberry plants respond to drought stress by altering the activities of antioxidant enzymes and the levels of osmotically active metabolites. These biochemical response changes may confer adaptation to drought stress and improve the capacity of plants to withstand water-deficit conditions.

• 한국수산과학회지
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• 제54권4호
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• pp.426-433
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• 2021

This study aimed to determine the appropriate salinity condition for optimal cultivation of the juvenile hybrid grouper Epinephleus akaara♀×E. lanceolatus♂ in conditions of decreasing salinity. The survival, growth, metabolic rate, stress, and histological response were investigated in juvenile hybrid grouper exposed to different salinities for 30 days. At 0 psu, the survival rate of the juvenile hybrid grouper was 60% on the 2nd day of exposure and all individuals died on the 3rd day of exposure. At salinities above 3 psu, all animals survived throughout the exposure period. Growth rate for body weight of the juvenile hybrid grouper was the highest in the control and the lowest at 10 psu. Plasma osmolality of the juvenile hybrid grouper exposed to different salinities was 313-355 mg Osmol/kg at salinities above 10 psu, and then decreased to 225-264 mg Osmol/kg at salinities below 5 psu. The oxygen consumption rate tended to decrease as the salinity decreased. Stress responses of the juvenile hybrid grouper were analyzed with decreasing salinity. Therefore, it is considered that a salinity of more than 20 psu is suitable for the culture of the juvenile hybrid grouper.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.108-108
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• 2022

In this study, multispectral images of wheat according to soil water state were collected, compared, and analyzed to measure the physiological response of crops to environmental stress at the individual level. CMS-V multi-spectral camera(Silios Technologies) was used for image acquisition. The camera lens consists of eight spectral bands between 550nm and 830nm. Light Reflective information collected in each band sensor and stored in digital values, and it is converted into a reflectance for calculating the vegetation index and used. According to the camera manual, the NDVI(Normalized Difference vegetation index) value was calculated using 628 nm and 752 nm bands. Image measurement was conducted under natural light conditions, and reflectance standards(Labsphere) were captured with plants for reflectance calculation. The wheat variety used Gosomil, and the wheat grown in the field was transplanted into a pot after heading date and measured. Three treatments were performed so that the soil volumetric water content of the pot was 13~17%, 20~23%, and 25%, and the growth response of wheat according to each treatment was compared using the NDVI value. In the first measurement after port transplantation, the difference in NDVI value according to treatment was not significant, but in the subsequent measurement, the NDVI value of the treatment with a water content of 13 to 17% was lowest and was the highest at 20 to 23%. The NDVI values decreased compared to the first measurement in all treatment, and the decrease was the largest at 13-17% water content and the smallest at 20-23%. Although the difference in NDVI values could be confirmed, it would be difficult to directly relate it to the water stress of plants, and further research on the response of crops to environmental stress and the analysis of multi-spectral image will be needed.

• 한국동물생명공학회지
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• 제34권3호
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• pp.173-182
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• 2019

This study was performed to evaluate the effect of heat stress on the status of physiological responses, blood parameter, serum T3 and cortisol, and heat shock proteins (HSP 27, 70, and 90) of Hanwoo cattle. Six Hanwoo steers (242.8 ± 7.2 kg of BW) were housed in the climate-controlled respiration chambers. The experiment consisted of 7 days (control; 0 day) at thermoneutral (air temperature (Ta) of 15℃ and relative humidity (RH) of 60%; temperature-humidity index (THI) = 64), and by 3 and 6 days (treatment groups) at heat stress (Ta of 35℃ and RH of 60%; THI = 87). Body temperature of each parts (frank, rump, perineum and foot) and rectal temperature elevated in heat stress groups (3 days and 6 days) than the control group (0 day). Respiration rates increased in 3 days and 6 days (88.5 ± 0.96 bpm and 86.3 ± 0.63 bpm, respectively) from 0 days (39.5 ± 0.65 bpm). Feed intake significantly decreased in heat stress groups (3 days and 6 days, 3.7 ± 0.14 kg and 4.0 ± 0.15 kg, respectively) than the control group (0 day, 5.0 ± 0.00 kg). In addition, final BW significantly decreased in heat stress groups (3 days and 6 days, 211.8 ± 4.75 kg and 215.5 ± 3.50 kg, respectively) than the control group (0 day, 240.0 ± 25.00 kg). However, heat stress has no significant effect on blood parameter, serum T3 and cortisol. Nevertheless, heat stress increased HSPs mRNA expression in liver tissue, and serum concentration of HSPs. Despite Hanwoo cattle may have high adaptive ability to heat stress, our results suggested that heat stress directly effect on body temperature and respiration rate as well as serum and tissue HSPs. Therefore, we are recommended that HSPs could be the most appropriate indicators of Hanwoo cattle response to heat stress.

• 한국의류학회지
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• 제44권4호
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• pp.776-787
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• 2020

This study investigated physiological and subjective responses to different types of firefighter station uniforms made with various designs and materials. Six healthy males participated in this study that consisted of 20 min of rest, 30 min of treadmill exercise, and 30 min of recovery in a hot and humid environment (34℃ and 65%RH). The experimental clothing conditions were as follows. 1) a fitted T-shirt and trouser made of 100% polyester (FC-Uniform), and 2) flame retardant T-shirts made of acrylic and cotton as well as trousers with aramid and polyester, designed for overfitting (Control). There were no significant differences in the body temperature, and sweat rate between the two conditions; however, the heart rate with the FC-Uniform was significantly lower than Control (p=.025). The clothing microclimate temperature at the chest of the FC-Uniform was significantly lower than the Control (p=.037), and a difference of 1℃ was maintained until the recovery was complete. There were no significant differences in the subjective responses; however, participants experienced a humidity sensation faster with FC-Uniform in the recovery phase. The results indicate that changes in the design and material of firefighter station uniforms may have a positive influence on reducing the thermal stress of firefighters.

• BMB Reports
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• 제42권7호
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• pp.421-426
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• 2009

Glucocorticoids regulate multiple physiological processes such as metabolic homeostasis and immune response. Mouse Pon2 (mPon2) acts as an antioxidant to reduce cellular oxidative stress in cells. In this present study, we investigated the transcriptional regulation of mPon2 by glucocorticoids. In the presence of glucocorticoid analogue dexamethasone, the expression of mPon2 mRNA in cells was increased, whereas the expression was inhibited by a transcription inhibitor actinomycin D. Glucocorticoid receptors bound to the putative glucocorticoid response elements located between -593 bp and -575 bp of the mPon2 promoter. Transcriptional activity was completely blocked when the putative element was mutated. Taken together, these results suggest that the expression of the mPon2 gene is directly regulated by glucocorticoid-glucocorticoid receptor complexes.

• The Korean Journal of Physiology
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• 제2권1호
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• pp.1-8
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• 1968

유두체의 기능을 탐색하기 위하여 외과 수술에 의하여 유두체를 떼어버린 개를 마련하고 수술전에서부터 수술후에 걸쳐 다음의 세가지 실험을 실시하여 그 성적을 비교하였다. 첫째 실험에서는 먹이 먹는 개의 혀에 전기 충격을 가한다음 이어서 동물이 다시 먹이 그릇에 접근하기까지의 시간을 측정하여 동물이 무서움을 타는정도를 짐작하였으며, 둘째 실험에서는 높은 소리 (12,000 cps, 100 db)를 한 시간 동안 동물에게 들려 스트레스로 삼고 혈액 호산구 수의 변동을 추적하여 스트레스에 대한 반응의 지표로 삼았다. 셋째 실험에서는 아침과 저녁 일정한 시간에 항문 온도를 반복 측정하였다. 실험 결과는 다음과 같다. 1. 유두체 제거후의 동물이 유두체 제거전 보다 무서움을 더 혹은 덜 탄다는 증거는 없었다(제 1 표). 2. 유두체가 제거된 후에도 스트레스에 대한 반응은 유두체 제거전과 같은 정도로 현저히 나타나나 스트레스에서의 회복과정은 유두체 제거전에 비하여 유두체 제거후에 유의하게 지연되었다(제 1 도). 3. 유두체가 제거된 동물의 체온은 유두체 제거전에 비하여 떨어지는데 그 정도는 미약하나 통계적으로 유의한 차이를 나타내었다(제 2 표).

• 환경생물
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• 제22권4호
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• pp.559-564
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• 2004

The effects of intra-peritoneal injection of inorganic mercury on haemato-logical parameters and hepatic oxidative stress enzyme activities were studied in common carp, Cyprinus carpio. The fish were injected thrice intra-peritoneally with mercuric chloride TEX>$(5,\;10mg\;Hg\;kg\;b.W.^{-1})$. After exposure of three different mercury concentrations a physiological stress response was exerted on C. carpio by causing changes in the blood status such as erythropenia in blood and oxidative stress in liver. Red blood cell counts, hemoglobin concentration and hematocrit level were reduced in most cases by inorganic mercury. Remarkable low level of serum chloride, calcium and osmolality were also observed in the mercury- exposed fish. However, serum magnesium and phosphate were not altered by exposure to mercury. An increased activity of hepatic glutathione peroxidase was observed in the lowest treatment group of carp $(1mg\;Hg\;mg\;b.w.^{-1})$, hence, hepatic catalase and glutathione peroxidase of carp exposed to higher concentration of mercury $(5,\;10mg\;Hg\;kg\;b.W.^{-1})$ showed significant reduction in such activities.

• ALGAE
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• 제26권1호
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• pp.3-20
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• 2011

The physiology of the unicellular green alga Dunaliella salina in response to abiotic stress has been studied for several decades. Early D. salina research focused on its remarkable salinity tolerance and ability, upon exposure to various abiotic stresses, to accumulate high concentrations of $\beta$-carotene and other carotenoid pigments valued highly as nutraceuticals. The simple life cycle and growth requirements of D. salina make this organism one of the large-scale commercially exploited microalgae for natural carotenoids. Recent advances in genomics and proteomics now allow investigation of abiotic stress responses at the molecular level. Detailed knowledge of isoprenoid biosynthesis mechanisms and the development of molecular tools and techniques for D. salina will allow the improvement of physiological characteristics of algal strains and the use of transgenic algae in bioreactors. Here we review D. salina isoprenoid and carotenoid biosynthesis regulation, and also the biotechnological and genetic transformation procedures developed for this alga that set the stage for its future use as a production system.