• Science of Emotion and Sensibility
• /
• v.18 no.3
• /
• pp.63-70
• /
• 2015

This study identified heart response of participants while listening to sounds which have 1/f fluctuations with exponent ${\alpha}$ gradient. The participants were engaged in emotional stress work. Prior studies related to 1/f fluctuation sound have reported that sound source can alleviate psychological and physiological state of users. Subjects of this study were exposed to sound with three levels of ${\alpha}$ gradient. Heart response of subjects were measured with Photoplethysmography(PPG) sensor simultaneously. The dependent variables of this study were beat per minute(BPM), very low frequency percent of pulse rate variability (VLF percent), the standard deviation of all normal RR intervals (SDNN), and high frequency power(HF power). Subject showed arousal response when exposed to sound with exponent ${\alpha}$ gradient of 3 whereas the sound with exponent ${\alpha}$ gradient of 1 and 2 resulted in relax effect. The characteristic of 1/f fluctuation sounds can be applied to alleviate stress for employers under emotional labor.

• Journal of Korean Academy of Nursing Administration
• /
• v.5 no.1
• /
• pp.149-167
• /
• 1999

This study was designed and carried out to investigate the effect of Assertiveness Training on the stress response and assertive behavior of emergency room nurses from September 22, to December 21. 1997. The subjects were 45 emergency room nurses of three general hospitals of Korea University Medical Center; 25 assigned for the experimental and 20 for the control group. The Assertive Training Program was composed of cognitive training, behavioral training and progressive muscle relaxation. The subjects of the experimental group carried out the 15-minute progressive muscle relaxation according to recorded-tape once a day for 6 weeks. The cognitive and the behavioural training were conducted by the investigator for 5-8 subjects at a time for 9 series, at an interval of 4-5 days. Post-measurement were administered to all sujects in two groups three weeks after the last session. The level of general stress (Cline, 1992), job stress (Kim & Koo, 1984), physiological response to stress (cortisol, epinephrine, norepinephrine), symptoms of stress (Lee, 1984) and assertive behavior (Kim, 1982) were measured before the first and after the last experiments. Data were analysed by x2, t, paired t, Wilcoxon signed rank tests Wilcoxon rank sums tests. The results are as follows : 1. The level of general stress of the experimental group was lower than that of the control group, however, no significant difference between the two groups (P=0.234) was revealed. 2. A significant reduction of job stress was revealed in the experimental group (P=0.017). 1) A significant reduction of interpersonal conflicts in the experimental group was revealed (P=0.018). 3. The physiological stress-response were analyzed as follows : 1) A decrease of Cortisol in both the experimental and the control groups was noticed, however, the difference between the two groups was not statistically significant (P=0.991). Pre-and post-measurement of the level of Corti sol revealed that a notable decrease in the experimental group and an increase in the control group with no significant difference in the day-day subjects (P=0.765), a significant decrease was noted on the level of Cortisol of the experimental group in the night-night nurses (P=0.036). 2) An increase of Epinephrine in both groups was noticed, however, a difference was not statistically significant between the two groups (P=0.082). Pre-and post-measurement of the level of Epinephrine revealed that a notable decrease in the experimental group and an increase in the control group with no significant difference in the day-day subjects (P=0.136). increases were noted on the level of Epinephrinel of both groups in the night-night nurses (P=0.136), 3) The level of Norepinephrine of the experimental group was significantly decreased (P=0.020). Pre-and post-measurement of the level of Norepinephrine revealed that a notable decrease in the experimental group and an increase in the control group with no significant difference in the day-day subjects (P=0.073). a significant decrease was noted on the level of Norepinephrine of the experimental group in the night-night nurses (P=0.036), 4. Symptoms of stress were reduced in both groups, with no significant differences between the groups (P=0.127). 1) The physical stress symptoms were reduced in both groups, with no significant difference (P=0.386), 2) The cognitive-behavioral stress symptoms were significantly reduced in the experimental group (P=0.037). 3) The emotional stress symptoms were reduced in both groups with no significant difference between the groups (P=0.110). 5. The assertive behavior was significantly increased in the experimental group (P=0.000). This study signified the Assertiveness Training as an effective mean of self intervention for the reduction of stress of nurses assigned to emergency rooms.

• Biotechnology and Bioprocess Engineering:BBE
• /
• v.8 no.6
• /
• pp.322-330
• /
• 2003

The unicellular green alga Haematococcus pluvialis has recently attracted great inter-est due to its large amounts of ketocarotenoid astaxanthin, 3,3'-dihydroxy-${\beta}$,${\beta}$-carotene-4,4'-dione, widely used commercially as a source of pigment for aquaculture. In the life cycle of H. pluvialis, astaxanthin biosynthesis is associated with a remarkable morphological change from green motile vegetative cells into red immotile cyst cells as the resting stage. In recent years we have studied this morphological process from two aspects: defining conditions governing astaxanthin biosynthesis and questioning the possible function of astaxanthin in protecting algal cells against environmental stress. Astaxanthin accumulation in cysts was induced by a variety of environmental conditions of oxidative stress caused by reactive oxygen species, intense light, drought, high salinity, and high temperature. In the adaptation to stress, abscisic acid induced by reactive oxygen species, would function as a hormone in algal morphogenesis from veget ative to cyst cells. Furthermore, measurements of both in vitro and in vivo antioxidative activities of astaxanthin clearly demonstrated that tolerance to excessive reactive oxygen species is greater in astaxanthin-rich cysts than in astaxanthin-poor cysts or astaxanthin-less vegetative cells. Therefore, reactive oxygen species are involved in the regulation of both algal morph O-genesis and carotenogenesis, and the accumulated astaxanthin in cysts can function as a protective agent against oxidative stress damage. In this study, the physiological roles of astaxanthin in stress response and cell protection are reviewed.

• Journal of Korean Critical Care Nursing
• /
• v.3 no.1
• /
• pp.41-51
• /
• 2010

Purpose: The purpose of this study was to identify the effects of family visits upon the stress response of patients and their families, Methods: This study was the interrupted time series design, The subjects consisted of 197 patients and 197 family members in the cardiac intensive care unit of S Hospital in Bucheon. Physiological stress responses such as blood pressure, heart rates, respiration rates, and oxygen saturation were measured using HP monitors. VAS was used to measure the emotional stress. Collected data was analyzed using repeated measure ANOVA, t-test by SPSS 17.0 statistical program. Results: The family visits did not change patients' blood pressure, pulse rate, respiration rate and oxygen saturation, However the anxiety level of patients and their family members were decreased significantly during family visits. Furthermore, 30-minute family visit reduced more effectively patient's anxiety than 15-minute family visit. Conclusion: Family visits need to be used as a means of nursing intervention to ease the emotional stress of patients and their families. In addition, increasing of visiting time should be considered.

• Journal of Microbiology and Biotechnology
• /
• v.11 no.3
• /
• pp.443-451
• /
• 2001

In order to investigate what kind of response anaerobic bifidobacteria has on oxygen stress, five oxygen-tolerant bifidobacteria were isolated from human fecal samples. All were temporarily identified as Bifidobacterium longum through an analysis of carbohydrate utilization patterns and cellular fatty acid profiles. In the presence of oxygen, the lag phase became extended and the cell growth was suppressed. Bifidobacterial cell was able to remove dissolved oxygen in an early stage of growth and to overcome oxygen stress to a certain extent. The cell became long n size and showed a rough surface containing many nodes which were derived from abnormal or incomplete cell division. Cellular fatty acid profiled changed remarkably under a partially aerobic condition, so that the carbon chain of cellular fatty acid became short. All the dimethyl acetals originated from plasmalogen were reduced, any cyclopropane fatty acid, 9, 10-methyleneoctadecanoic acid ($C_{19:0}cyc9,10$), was increased remarkably. Oxygen stress induced a 5.5 kD protein in B. longum JI 1 of the oxygen-teolerant bifidobacteria, that was named Osp protein, and its N-terminal amino acid sequence was as follows: unknown amino acid-Thr-Gly-Val-Arg-Phe-Ser-Asp-Asp-Glu. Therefore, the oxygen-tolerant bifidobacteria seemed to defend against oxygen stress byincreasing the content of short fatty acid and cyclopropane fatty acid, and induction of an oxygen stress protein, but not the plasmalogen.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.40 no.5
• /
• pp.594-599
• /
• 1995

Generally, excessive soil water stress in vegetative growth stage inhibits the growth of soybeans. Leaf area expansion of the plant during excessive soil water stress was only half and the respiration of roots was much diminished compared with the plant none water stress. When excessive soil water stress to the soybeans was continued for 7 days, outer epidermis and vascular system of tap root were severely cracked, more than thirty-five percent of nodule was died and the bacteroid layers of alive nodule were disintegrated.

• Korean Journal of Poultry Science
• /
• v.38 no.4
• /
• pp.305-313
• /
• 2011

This study was conducted to investigate the effects of housing systems on the productivity and physiological response as stress indicators in White Leghorn chickens. The chickens subjected to the conventional cages had a significantly lower viability, hen-housed egg production, egg weight and body weight compared with those to the floor pens. However, the hens housed in the conventional cages had a shorter day of the first egg and a greater egg quality compared with those housed in the floor pens. In addition, this study was also investigated to identify biological markers for assessing the physiological response of chickens under stress conditions. As biological markers, the amount of telomeric DNA was analyzed by quantitative fluorescent in situ hybridization on the nuclei of cells. The DNA damage rate of lymphocytes was also quantified by the comet assay. The amount of telomeric DNA of the lymphocytes, kidney and spleen was significantly higher in the chickens under floor pens than those under conventional cages. The DNA damage also increased in chickens raised under conventional cages, as compared to the chickens under floor pens. As results, we conclude that the chickens housed in conventional cages have a greater stressful status than those housed in floor pens.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2017.06a
• /
• pp.128-128
• /
• 2017

Copper (Cu) is very toxic to plant cells due to its inhibitory effects on many physiological and biochemical processes. In spite of its potential physiological and economic significance, molecular characterization after Cu stress has so far been grossly overlooked in sorghum. To explore the molecular alterations that occur in response to copper stress, the present study was executed in ten-day-old Cu-exposed leaves of sorghum seedlings. The growth of shoots was markedly reduced, and ionic alterations were prominently observed in the leaves when the seedlings were exposed to different concentrations (0, 100, and $150{\mu}M$) of $CuSO_4$. Using two-dimensional gels with silver staining, 643 differentially expressed protein spots (${\geq}1.5-fold$) were identified as either significantly increased or reduced in abundance. Of these spots, a total of 24 protein spots (${\geq}1.5-fold$) from Cu-exposed sorghum leaves were successfully analyzed by MALDI-TOF-TOF mass spectrometry. Of the 24 differentially expressed proteins from Cu-exposed sorghum leaves, a total of 13 proteins were up-regulated, and 11 proteins were down-regulated. The abundance of most identified protein species, which function in carbohydrate metabolism, stress defense, and protein translation, was significantly enhanced, while that of another protein species involved in energy metabolism, photosynthesis and growth and development were severely reduced. The resulting differences in protein expression patterns together with related morpho-physiological processes suggested that these results could help to elucidate plant adaptation to Cu stress and provide insights into the molecular mechanisms of Cu responses in $C_4$ plants. The over-expression of GAPDH plays a significant role in assisting Sorghum bicolor to attenuate the adverse effects of oxidative stress caused by Cu, and the proteins involved in resistance to stress helped the sorghum plants to tolerate high levels of Cu.

• Research in Community and Public Health Nursing
• /
• v.6 no.1
• /
• pp.98-113
• /
• 1995

The purpose of this study were two fold : first, to determine the effect of aerobic exercise on BP, pulse rate, body fat, body weight, symptoms of sterss response: and secondly, to apply the aerobic exercise on nursing practice in the healthy or ill subjects. In an attempt to investigate the physiological and psychological effect of exercise, a quasi -experiment, non-equivalent control group pre-test & post-test design was planned. Experiments were carried out from July 4, through August 30, 1994 with 37 subjects conveniently sampled from K & Y sports center located in Taejon. The 37 research subjects were assigned to experimental(16 subjects) and control(21 subjects) groups. Aerobic exercise was carried out for experimental group from three times to five times a week for 6 weeks in aerobic center. Data were analyzed with t-test, mean, per centage of change using SAS program. Results were obtained as follows: 1) Systolic blood pressure (M=123.75mmHg, % of change=1.12%) and diastolic blood pressure (M=74.38mmHg, % of change=5.79%) was increased on that of experimental group after 6 weeks experiment. But there was no significant difference between experimental group and control group. 2) Body weight was decreased on that of experimental group(M=56.54Kg, % of change=-1.60%) and that of control group(M=52.05, % of change=-0.99%) after 6 weeks. But there was no significant difference between experimental group and control group. 3) Body fat was decreased on that of experimental group(M=30.53%, % of change=-3.60%) and that of control group (M=28.75%, % of change=-3.52%) after 6 weeks. There was no significant difference between experimental group and control group. 4) Pulse rate was decreased on that of experimental group(M=69.19회/min, % of change -8.43%) and that of control group(M=76.0회/min, % change -0.07%) after 6weeks. There was significant difference(t=-2.621, P<0.05) between experimental group and control group. 5) Symptoms of stress response were decreased on that of experimental group(M=0.97) and that of control group(M=1.15) after 6 weeks. There was no significant difference between experimental group and control group. Therefore, adherence to aerobic exercise for 6 week seem to be affected mainly pulse rate of subjects. A futher study is necessary to determine the difference in the effect of variety exercise, programs, to study over 6 weeks, to produce professional educational program for exercise speciality nurses.

• Korean Journal of Ichthyology
• /
• v.17 no.1
• /
• pp.43-48
• /
• 2005

We investigated physiological responses of hybrid striped bass (Morone chrysops ${\times}$ M. saxatilis) to gradual changes of salinity from 33 psu to 0 psu and from 0 psu to 33 psu in two separate trials. Gradual salinity change was not accompanied by an increase in cortisol level in the plasma however, the glucose level in the plasma increased. $Na^{+}$, $Cl^{-}$ and osmolality significantly increased with rising salinity, but they did not change with dropping salinity. The hybrid striped bass adjusted effectively without stress to the gentle change of salinity.