• ALGAE
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• v.29 no.3
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• pp.183-195
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• 2014

There are recognized three new species of Polysiphonia sensu lato at a 10-20 m water depth from Jeju Island, Korea. We described the morphology of three new species and analyzed rbcL sequences to infer phylogenetic relationships among Polysiphonia sensu lato. Polysiphonia amplacapilli sp. nov. is characterized by an epiphytic, discoid holdfast, 5-7 pericentral cells, large trichoblasts, lateral branches connecting with trichoblasts, only slightly corticated in the basal parts, cut-off rhizoids and a spiral arrangement of tetrasporangia. Polysiphonia morroides sp. nov. is characterized by saxicolous, cut-off rhizoids, four pericentral cells, ecorticate, spiral arrangement of tetrasporangia, and it was collected at the fast-flowing water habitat in 20-25 m depth. Polysiphonia sabulosia sp. nov. is characterized by ecorticate, 5-6 pericentral cells, a single filamentous trichoblast, lateral branches connecting with the trichoblasts, cut-off rhizoids, and spiral arrangement of tetrasporangia, and is growing in the sandy locations. The phylogenetic analysis of rbcL sequences demonstrated that three new species are clearly distinguished from other species of Polysiphonia sensu lato.

• Animal Systematics, Evolution and Diversity
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• v.30 no.2
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• pp.87-94
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• 2014

Specimens of the genus Orobdella Oka, 1895 from Korea, including various locations in the Korean Peninsula, were identified as Orobdella tsushimensis Nakano, 2011. Phylogenetic analyses using mitochondrial cytochrome oxidase subunit 1 (COI), ND1, $tRNA^{Cys}$, $tRNA^{Met}$, 12S rRNA, $tRNA^{val}$, and 16S rRNA markers show that the newly collected specimens form a monophyletic group with the known O. tsushimensis specimens. The genetic distance of COI of these specimens was in the range 0.4-6.6%. These results confirm that the newly collected specimens belong to O. tsushimensis. This is the first record of the genus Orobdella from the Korean Peninsula.

• Korean Journal of Medicinal Crop Science
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• v.14 no.6
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• pp.342-346
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• 2006

Anatomical characters of the Bupleurum latissimum Nakai, an endemic species of Korea, were investigated to confirm its phylogenetic relationships. Compare to other species with anatomical characters, B. latissimum is very similar with B, euphorbioides and B, longeradiatum in point of lacking of pith in the stem, shape of involucres, number of vascular bundles in radical leaf and cauline leaf, and lacking stomata in adaxial leaf surface. The other hand, protruded pollen aperture character appears in B. latissimum and B. euphorbioides. On the based of anatomical characters, therefore, B. latissimum has closest relationships with B. euphorbioides and B. longeradiatum. It also needs molecular study including Asian species in order to confirm phylogenetic position and speciation process apparently.

• Mycobiology
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• v.42 no.3
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• pp.282-285
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• 2014

A fungal isolate EML-NCP01 was recovered from agricultural soil in Ulleung Island, Korea. Phylogenetic analysis of internal transcribed spacer and ${\beta}$-tubulin genes identified the isolate as the Penicillium species P. raphiae. Morphologically, the EML-NCP01 isolate was identical to the previous description of P. raphiae. The species presented here has not been reported in Korea.

• The Korean Journal of Mycology
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• v.45 no.3
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• pp.224-228
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• 2017

The fungal strain, designated KNU16-008, was isolated from field soil in Chungcheongnam-do, Korea. The isolated fungi was characterized by morphological and phylogenetic analyses. Isolated fungus showed typical morphological characteristics of the genus Sporothrix. Based on its phylogenic analysis using internal transcribed spacer (ITS) of rDNA and ${\beta}$-tubulin gene sequences, the strain KNU16-008 was identified as Sporothrix stylites. This species has not been previously reported in Korea.

• Mycobiology
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• v.48 no.3
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• pp.233-239
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• 2020

A small-spored Alternaria was found from black spots of storaged Koerle pear (Pyrus sinkiangensis), one of the economically important fruit in Xinjiang province, China. The morphology is similar to A. limoniasperae but obviously different in secondary conidiophores and conidial septa. A phylogenetic analysis using sequence datasets of ITS, GAPDH, TEF1, RPB2, Alt a1, OPA10-2, and EndoPG genes revealed that it belonged to the Alternaria alternata complex group. Pathogenicity tests illustrated that the fungus was the causal pathogen of black spot on Koerle pear fruit.

• International Journal of Industrial Entomology and Biomaterials
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• v.10 no.2
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• pp.79-86
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• 2005

Molecular markers have increasingly been used in plant genetic analysis, due to their obvious advantages over conventional phenotypic markers, as they are highly polymorphic, more in number, stable across different developmental stages, neutral to selection and least influenced by environmental factors. Among the PCR based marker techniques, ISSR is one of the simplest and widely used techniques, which involves amplification of DNA segment present at an amplifiable distance in between two identical microsatellite repeat regions oriented in opposite direction. Though ISSR markers are dominant like RAPD, they are more stable and reproducible. Because of these properties ISSR markers have recently been found using extensively for finger printing, pohylogenetic analysis, population structure analysis, varietal/line identification, genetic mapping, marker-assisted selection, etc. In mulberry (Morus spp.), ISSR markers were used for analyzing phylogenetic relationship among cultivated varieties, between tropical and temperate mulberry, for solving the vexed problem of identifying taxonomic positions of genotypes, for identifying markers associated with leaf yield attributing characters. As ISSR markers are one of the cheapest and easiest marker systems with high efficiency in generating polymorphism among closely related varieties, they would play a major role in mulberry genome analysis in the future.

• Journal of Applied Biological Chemistry
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• v.51 no.6
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• pp.285-291
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• 2008

A potential probiotics bacterial strain, CS90, was isolated from Korean soybean paste (doenjang). The strain CS90 showed antimicrobial activity against food-borne pathogenic bacteria including Salmonella enterica, Salmonella enteritids, Salmonella typhymurium, Bacillus cereus, Listeria ivanovii, Listeria. monocytogenes, Sthaphylococcus aureus, and Sthaphylococcus epidermidis and showed a significant survival rate of 35.7 to 57.8% under the artificial gastric acidic condition (pH 2 to 3). The strain CS90 was classified as Bacillus subtilis based on morphological, physiological, chemotaxonomic features and phylogenetic analysis based on 16S rDNA sequence and designated as B. subtilis CS90. B. subtilis CS90 can be used as a potential probiotics.

• Journal of Microbiology and Biotechnology
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• v.13 no.3
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• pp.406-414
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• 2003

Phylogenetic relationships of stereoid fungi were examined by comparing nuclear small subunit ribosomal RNA gene sequences. Stereoid taxa were scattered into several groups and the traditional Stereaceae proved to be polyphyletic. Srereum and Xylobolus were classified in the Stereaceae as the core group of stereoid fungi, and Amylostereum was grouped with Echinodontium of the Echinodontiaceae. Chondrostereum and Cystosrereum were clustered in the Stereaceae sensu Donk and Cymatoderma and Podoscypha in the Podoscyphaceae Reid. Columnocystis abietinum and C. ambigua were grouped with Meripilus giganteus and proved to be not included in the Chaetodermataceae sensu Nakasone. Lopharia cinerascens and L. mirabilis were grouped together but L. spadicea was unrelated to them, indicating that Lopharia is heterogeneous at a generic level.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.5
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• pp.743-747
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• 2003

Applying simple random sampling in typical colony methods in the central area of habitat, 14 structural loci and 31 alleles in blood enzyme and other protein variations of Hu sheep population are examined. After collecting the same data of 11 loci about the 22 sheep colonies in China and other countries, it clusters the 23 sheep populations by fuzzy cluster analysis. The study proves that the phylogenetic relationship between Hu sheep population and Mongolia populations is relatively closed. This result obtained is shown to conform to the historical data.