This study was to evaluate the effects of gamma irradiation on the germination, nutrient concentrations and growth of spinach and radish. Both the spinach and radish seeds exhibited relatively higher germination rates in response to the low doses of gamma irradiation compared to the non -irradiated control. Leaf DW of the radish did not respond to gamma irradiation but that of the spinach increased significantly in response to a gamma radiation of 4 Gy (P< 0.05). Leaf growth parameters of the spinach including the leaf area and SLA (leaf area/leaf dry weight) also demonstrated increased responses to gamma irradiation. R/S (root dry weight/shoot dry weight), root DW and root length of the spinach exhibited a positive response to gamma irradiation while those of the radish did not. In contrast, SRL (root length/root dry weight) significantly decreased with gamma irradiation at 8 Gy for the spinach, but not for the radish. The tissue nitrogen concentrations of the spinach showed an increased response to gamma irradiation while that of the radish did not. Furthermore, higher concentrations of phosphorus, potassium, calcium and magnesium were found in the irradiated spinach, but not in the irradiated radish. It seems that the non-specific physiological and/or biochemical activities of spinach might be accelerated by gamma irradiation, possibly accounting for the stimulation of nutrient uptake from the root media and early biomass accumulation in the current study.
Heavy metal remediation in shooting range soil is a challenge over the world. The excessive Pb accumulation in the soil can deteriorate soil quality and fertility. The objectives of this research were to evaluate the efficiency of biochar (BC) in improving the physicochemical and biological properties of the soil and to evaluate its effect on Pb availability in a military shooting range soil. Sandy loam soil was collected from shooting range of Gyeonggi Province, South Korea and was incubated for 30 days with different application rates (0-30% w $w^{-1}$) of BC. The results showed that the addition of BC increased aggregate stability, nitrogen (N) and phosphorus (P) contents, and enzyme activities in soil. Sequential extraction showed that residual and organic bound fractions in the soil amended with BC increased by 33.1 and 16.7%, respectively, and the exchangeable fraction decreased by 93.7% in the soil amended with BC, compared to the unamended soil. We concluded that the application of BC could not only improve physicochemical and biological soil qualities but also stabilize Pb in a shooting range soil.
Recently, the population growth, industrial and agricultural development are rapidly undergoing in the Lower Rio Grande Valley (LRGV) in Texas. The Lower Rio Grande Valley (LRGV) composed of the 4 counties and three of them are interesting for Non-point and point source pollutant modeling: Starr, Cameron, and Hidalgo. Especially, the LRGV is an intensively irrigation region, and Texas A&M University Agriculture Program and the New Mexico State University College of Agriculture applied irrigation district program, projects in GIS and Hydrology based agricultural water management systems and assessment of prioritized protecting stream network, water quality and rehabilitation based on water saving potential in Rio Grande River. In the LRGV region, where point and non-point sources of pollution may be a big concern, because increasing fertilizers and pesticides use and population cause. This project objective seeks to determine the accumulation of non-point and point source and discuss the main impacts of agriculture and environmental concern with water quality related to pesticides, fertilizer, and nutrients within LRGV region. The GIS technique is widely used and developed for the assessment of non-point source pollution in LRGV region. This project shows the losses in $kg/km^2/yr$ of BOD (Biological Oxygen Demand), TN (total Nitrogen) and TP (total phosphorus) in the runoff from the surface of LRGV. Especially, farmers in Cameron County consume a lot of fertilizer and pesticide to improve crop yield net profit. Then, this region can be created as larger nonpoint source area for nutrients and the intensity of runoff by excess irrigation water. And many sediment and used irrigation water with including high nutrients can be discharged into Rio Grade River.
Recently, the population growth and agricultural development are rapidly undergoing in the South-West Texas. The junction of three river basins such as Lavaca river basin, Colorado-Lavaca Coastal basin and Lavaca-Guadalupe Coastal basin, are interesting for non-point and point source pollutant modeling: Especially, the 2 basins are an intensively agricultural region (Colorado-Lavaca Coastal/Lavaca-Guadalupe Coastal basins) and several cities are rapidly extended. In case of the Lavaca river basin, there are many range land. Several habitat types wide-spread over three relatively larger basins and five wastewater discharge regions are located in there. There are different hazardous substances which have been released. Total nutrient loads are composed of land surface load and river load as Non-point source and discharge from wastewater facilities as point source. In 3 basins region, where point and non-point sources of poll Jtion may be a big concern, because increasing fertilizers and pesticides use and population cause. This project objective seeks to how to assess and control the accumulation of non-point and point source and discuss the main impacts of agriculture and environmental concern as non-point source with water quality related to pesticides, fertilizer, and nutrients and as point source with wasterwater discharge from cities. The GIS technique has been developed to aid in the point and non-point source analysis of impacts to natural resource within watershed. This project shows the losses in $kg/km^2/year$ of BOD (Biological Oxygen Demand), TN (Total Nitrogen) and TP (Total Phosphorus) in the runoff from the surface of 3 basins. In the next paper, sediment contamination will show how to evaluate in Estuarine habitats of these downstream.
Decomposition of green manure is necessary for the nutrient supply in farm soil. Hairy vetch as a green manure is superior to other winter legumes in terms of wintering ability and N accumulation. This experiment was carried out to investigate the decomposition and N release of hairy vetch and its effect on rice production as the following crop in paddy field. Decomposition of hairy vetch placed by soil depth of 0, 10 and 20cm at transplanting time was investigated by mesh bag method, which was enclosed chopped residue in mesh bags. The fate of $^{15}$ N derived from $^{15}$ N-labeled hairy vetch was investigated at harvest in three levels of N fertilization. Grain yield of the transplanted paddy rice cultured with hairy vetch as starter N were compared with that of applying urea as starter N in the field. Hairy vetch residue decomposed very rapidly both in transplanted and dry-seeded paddy field. In transplanted paddy field, hairy vetch residue lost 72-81 % and 86-90% of its weight after one and five month, respectively, as affected by incorporation depth. The C/N ratio of the decomposing vetch residue increased sharply during the early stages and after then, decreased slowly. The amounts of N and P released from the vetch were about 90% and 97% of initial content after one month, respectively. Recoveries of hairy vetch-$^{15}$ N by rice plant were 30.6, 34.6 and 35.7% in 0, 6 and 12 kg urea-N 10 $a^{-l}$ application, respectively, indicating that N fertilization increased the recovery of hairy vetch. $^{15}$ N. Hairy vetch residue incorporated as starter maintained significant N $H_4$$^{+}$-N concentration in soil water of plow layer until effective tillering stage. Grain yield in the plot applied with hairy vetch was not significantly different from that in the plot with urea. We concluded that hairy vetch incorporation could substitute starter N fertilization and showed possibility to reduce N amount of top-dressing.g.g.
Aerobic granular sludge (AGS) can be classified as a type of self-immobilized microbial aggregates measuring more than 0.2 mm. It offers the option to simultaneously remove COD, N, and P that occur in different zones inside a granule. Also, AGS is characterized by high precipitability, treatability with high organic loading, and high tolerance to low temperature. In this study, a sequencing batch reactor inoculated with AGS (AGS-SBR) is a new advanced wastewater treatment process that was proven to grow AGS with integrated nutrient removal and low C/N ratio. A pilot plant, AGS-SBR with a capacity of 225 ㎥/d was installed at an S sewage treatment plant in Gyeonggi-do. The results of the operation showed that the water quality of the effluent indicated that the value of BOD5 was 1.5 mg/L, CODMn was 11.4 mg/L, SS was 6.2 mg/L, T-N was 13.2 mg/L, and T-P was 0.197 mg/L, and all of these values reliably satisfied an effluent standard (I Area). In winter, the T-N treatment efficiency at a lower temperature of less than 11℃ also showed reliability to meet the effluent standard of the I Area (20 mg/L or less). Analysis of microbial community in AGS showed a higher preponderance of beneficial microorganisms involved in denitrification and phosphorus accumulation compared with activated sludge. The power consumption and sludge disposal cost were reduced by 34.7% and 54.9%, respectively, compared to the domestic SBR type sewage treatment plant with a processing capacity of 1,000 ㎥/d or less.
To evaluate the impact of effluents from land-based fish farms on the coastal ocean of Wando, Korea, we analyzed inorganic nutrients, particulate organic carbon (POC), dissolved organic carbon (DOC), and colored dissolved organic matter (CDOM) in the effluent and influent of land-based fish farms during the summer (July) of 2021. The average concentrations of nutrients (Dissolved inorganic nitrogen, phosphorus, and silicate; DIN, DIP, and DSi, respectively) in the effluents of this study area were 17±3.7 μM, 1.4±0.7 μM, and 14±1.6 μM, respectively. The average concentrations of POC and DOC were 37±22 μM and 81±13 μM, respectively, with POC accounting for about 30% for total organic carbon in effluents. The Reduced Dissolved Inorganic Nitrogen/Total Dissolved Inorganic Nitrogen ratio (0.7), potential short-period index, indicates that the discharge of nutrients excreted by the fish and unconsumed feed into coastal water results in such nutrients being deposited and accumulated in the sediment. Subsequently, this continuous accumulation triggers the release of ammonium ions during organic matter decomposition, and the ammonium-enriched waters that encroach on fish farms as influent seem to be due to the diffusion of high concentrations of ammonium from bottom sediment. Furthermore, we used fluorescence indices to examine the characteristics of organic matter sources, obtaining mean values of 1.54±0.19, 1.06±0.06, and 1.56±0.06 for the humification index, biological index, and fluorescence index, respectively, in the effluent. These results indicate that the organic matters had an autochthonous origin that resulted from microbial decomposition, and such organic matters were rapidly generated and removed by biological activity, likely supplied from the sediment. Our results suggest that the effluent from land-based fish farms could be a potential source of deoxygenation occurrence in coastal areas.
Park, Byung-Bae;Byun, Jae-Kyung;Kim, Woo-Sung;Sung, Joo-Han
Journal of Korean Society of Forest Science
/
v.99
no.1
/
pp.85-95
/
2010
The purpose of this study was to quantitatively measure both growth performances and nutrient responses of Fraxinus rhynchophylla, Fraxinus mandshurica, Pinus koraiensis, and Abies holophylla seedlings, which are commercially planted in Korea, to nitrogen, phosphorus, and potassium fertilization. We used Dickson's quality index (QI) to compare growth performances and vector diagnosis to interpret nutrient status. Nitrogen fertilization increased more height and root collar diameter growth in F. rhynchophylla and F. mandshurica relative to no fertilization treatment. The QI of F. rhynchophylla and F. mandshurica was the highest on N treatment, but there were no significant differences between treatments for P. koraiensis and A. holophylla. Nitrogen fertilization increased total dry weight by 43, 41, 26, -9% for F. rhynchophylla, F. mandshurica, P. koraiensis and A. holophylla, respectively. In F. rhynchophylla, N fertilization increased N contents with similar N concentrations ("sufficiency"), decreased both P concentrations and P contents ("antagonism"), and decreased K contents with similar K concentrations ("toxic accumulation"). In P. koraiensis, N fertilization decreased N, P, and K concentrations because of more dry weight increases compared to uptaken contents ("dilution"), but N fertilization decreased N, P, and K contents with similar N, P, and K concentrations ("toxic accumulation"). In the light of quality index and vector diagnosis, F. rhynchophylla and F. mandshurica seedlings treated with N fertilization would have high field performance.
Park, Seong-Joo;Yoon, Jerng-Chang;Shin, Kwang-Soo;Kim, Eung-Ho;Yim, Soo-Bin;Cho, Yeon-Je;Sung, Gi-Moon;Lee, Dong-Geun;Kim, Seung-Bum;Lee, Dong-Uk;Woo, Sung-Hoon;Koopman, Ben
Journal of Microbiology
/
v.45
no.2
/
pp.113-121
/
2007
The bacterial diversity inherent to the biofilm community structure of a modified rotating biological contactor wastewater treatment process, referred to as the Rotating Activated Bacillus Contactor (RABC) process, was characterized in this study, via both culture-dependent and culture-independent methods. On the basis of culture-dependent methods, Bacillus sp. were found to exist in large numbers on the biofilm (6.5% of the heterotrophic bacteria) and the microbial composition of the biofilms was quite simple. Only three phyla were identified-namely, the Proteobacteria, the Actinobacteria (High G+C Gram-positive bacteria), and the Firmicutes (Low G+C Gram-positive bacteria). The culture-independent partial 16S rDNA sequence analysis revealed a considerably more diverse microbial composition within the biofilms. A total of eight phyla were recovered in this case, three of which were major groups: the Firmicutes (43.9%), the Proteobacteria (28.6%), and the Bacteroidetes (17.6%). The remaining five phyla were minor groups: the Planctomycetes (4.4%), the Chlorobi (2.2%), the Actinobacteria (1.1%), the Nitrospirae (1.1%), and the Verrucomicrobia (1.1%). The two most abundant genera detected were the endospore-forming bacteria (31.8%), Clostridium and Bacillus, both of which are members of the Firmicutes phylum. This finding indicates that these endospore-forming bacteria successfully colonized and dominated the RABC process biofilms. Many of the colonies or clones recovered from the biofilms evidenced significantly high homology in the 16S rDNA sequences of bacteria stored in databases associated with advanced wastewater treatment capabilities, including nitrification and denitrification, phosphorus accumulation, the removal of volatile odors, and the removal of chlorohydrocarbons or heavy metals. The microbial community structures observed in the biofilms were found to correlate nicely with the enhanced performance of advanced wastewater treatment protocols.
This study was performed to investigate the effect of zeolite on the reduction of soil EC level in the plastic film house. The EC level of experimental soil was 5.0 dS $m^{-1}$ and the zeolite was applied to the soil at seven levels (0.5, 1, 2, 5, 10, 15, 20%) with three replications. The reduction degree of soil EC level showed positive tendency to the mixing ratio of zeolite. Especially, the EC level reduced rapidly from 5.01 to 2.8 dS $m^{-1}$ in the plot where zeolite was mixed by 20% 10 days after treatment. The pH level of soil was in positive relation to the mixing ratio of zeolite, contrary to the negative relation to the concentration level of water soluble Ca, Mg and phosphorus (P). The water contents of soil mixed with 15% and 20% zeolite were 14% and 17.3% respectively but it was 12.7% for control soil. Therefore, we expect natural zeolite to salts reduction agent for exchangeable cation and phosphate which is difficult to reduce by watering and other methods.
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