• 한국식품과학회지
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• 제15권2호
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• pp.195-201
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• 1983

3가지 보리로부터 추출한 지방질의 조성을 관, 얇은 막 및 기체-액체 크로마토그래피에 의하여 분석하였다. 총 지방질의 조성은 중성지질 69.3-73.1%, 당지질 9.6-16.5%, 인지질 14.2-17.9%였다. 중성지질 중에는 트리글리세리드의 함량이 가장 많았고 (중성지질의 54.2-55.7%), 그 외에 소량의 1, 2-및 1, 3- 디글리세리드, 유리스테롤, 유리지방산, 스테릴에스테르와 미동정한 3가지 지방질을 함유하였다. 당지질 중에는 디-갈락토오실 디-글리세리드와 모노 갈락토오실 디-글리세리드가 주성분이였고, 그 외에 에스테르화 스테릴 글리세리드, 스테릴 글리세리드, 세레 브로시드, 황지질 및 미동정한 한 가지 지방질이 주성분으로 존재하였다. 인지질 중에는 포스파티딜 콜린과세린, 라이소 포스파티딜 콜린, 포스파티딜 에탄올 아민이 전인지질의 80% 이상을 차지하는 주성분이었다. 총지방질 및 3가지 지방질 획분의 주요 지방산은 팔미트산, 올레산, 리놀레산 및 리놀렌산이었다. 그러나 중성지질 획분은 다른 지방질 획분보다 올레산의 함량이 많았고, 인지질 획분은 다른 지방질 획분보다 팔미트산의 함량이 많았다.

• 한국독성학회:학술대회논문집
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• 한국독성학회 1997년도 국제 심포지움 및 춘계 학술대회
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• pp.65-74
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• 1997

By using guinea pig lung mast cells, this study aimed to examine the effects of Aloe component(NY945) on the mediator releases caused by mast cell activation, and also aimed to assess the effects of NY945 on the mechanism of mediator releases in the mast cell activation. We partially purified mast cells from guinea pig lung tissues by using the enzyme digestion, the rough and the discontinuous density percoll gradient method. Mast cells were sensitized with $IgG_1$ (anti-OA) and challenged with ovalbumin. Histamine was assayed by fluorometric analyzer, leukotrienes by radioimmunoassay The phospholipase D activity was assessed more directly by the production of labeled phosphatidylethanol or phosphatidylbutanol which was produced by phospholipase D-mediated transphosphatidylation in the presence of ethanol or butanol. The amount of mass 1,2-diacylglycerol was measured by the [$^3H$]1,2-diacylgycerol produced when prelabeled with [$^3H$]myristic acid. In the mast cells prelabeled with L-[$^3H$]methyl methionine the phospholipid methylation was assessed by measuring the incorporation of the [$^3H$]methyl moiety into phospholipids. Pretreatment of NY945(10$\mu$g) significantly decreased histamine and leukotrienes releases during mast cell activation. The decrease of histamine release was stronger than that of leukotrienes during mast cell activation. The phospholipase D activity increased by the mast cell activation was decreased by the dose-dependent manner in the pretreatment of NY945. The amount of mass 1,2-diacylglycerol produced by activation of mast cells were decreased in the pretreatment of NY945. NY945 pretreatment strongly inhibited the incorporation of the [$^3H$]methyl moiety into phospholipids. The data suggest that NY945 purified from Aloe inhibits in part an increase of 1,2-diacylglycerol which is produced by activating mast cells with antigen-antibody complexes which is mediated via phosphatidylcholine-phospholipise D and phosphatidylinositole-phospholipise C systems, and then followed by the inhibition of histamine release. Furthermore, NY945 reduces the phosphatidylcholine production by inhibiting the methyltransfsrase I and II, which decrease the conversion of phosphatidylcholine into arachidonic acid and inhibits the production of leukotrines.

• 한국식품영양과학회:학술대회논문집
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• 한국식품영양과학회 2004년도 Annual Meeting and International Symposium
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• pp.47-52
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• 2004

This study was conducted to determine the effects of egg phospholipids [(Phosphatidylcholine (PC) and sphingomyelin (SM)] on intestinal absorption of cholesterol and other lipids. Each rat with Iymph cannula was infused via a duodenal catheter at 3.0 mL/h for 8 h with a lipid emulsion containing triolein, cholesterol and PC in 24 mL PBS. The PC in the lipid emulsion was egg PC (EPC), hydrogenated egg PC (HPC), or soy PC (SPC). The EPC in the lipid emulsion markedly lowered the Iymphatic absorption of cholesterol, compared with SPC and a lipid emulsion containing no PC. The HPC further lowered the absorption of cholesterol. The phospholipid output was not affected by the source of PC infused. The total Iymphatic output of oleic acid (18: 1), the major fatty acid infused in the form of triolein, did not differ among the NPC, SPC and EPC groups, but was significantly lower in the HPC group. The findings provide the first evidence that EPC markedly lowers the Iymphatic absorption of cholesterol under in vivo conditions. The inhibitory effect of EPC appears to be due to the higher degree of saturation of its acyl groups relative to SPC, suggesting that the intestinal absorption of egg cholesterol may be reduced by the presence of PC in e99 yolk. Experiment 2 was designed to determine whether egg SM, structurally similar to PC, also inhibits the Iymphatic absorption of cholesterol. Egg SM lowered the Lymphatic absorption of cholesterol in a dose dependent manner. Likewise, SM lowered the Iymphatic absorption of oleic acid, whereas it had no effect on retinol absorption. SM at a high dose lowered the Iymphatic outputs of both PC and SM, whereas there was no such effect at a lower dose. These results also indicate that luminal egg SM has an inhibitory effect on the intestinal absorption of cholesterol and other lipids of relatively high hydrophobicity.

• 센서학회지
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• 제3권2호
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• pp.33-39
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• 1994

냄새물질 특히, 환경오염물질의 측정을 위한 수정진동자를 이용한 센서 시스템을 수정진동자 표면에 여러가지 인지질과 활성탄 그리고 납계열 무기안료를 피막시켜 만들었다. 포스파티딜 콜린을 누적한 9MHz 수정진동자의 각종 유기가스에 대한 응답 특성을 살펴보았다. 인지질막의 특성에 기초한 서로 다른 냄새물질사이의 친화력에 대하여 설명할 수 있었다. 냄새물질은 누적한 지질막에 의존하는 공진주파수 변화 패턴과 응답면적에서 계산되어지는 상대적인 응답 세기를 서로 비교하여 확인할 수 있었다. 납계열 무기안료를 누적한 수정진동자를 이용하여 황화물의 검출이 가능함을 알 수 있었다. 이 결과, 여러가지 지질을 누적한 수정진동자를 이용하여 그 응답패턴을 비교한다면 냄새물질의 분석이 가능함을 알았다.

• 한국식품조리과학회지
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• 제13권2호
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• pp.87-91
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• 1997

방사구와 비방사구에서 채취한 달걀 난황을 배소·압착하여 얻은 난황유를 Folch법으로 정제한 후 gas chromatography로 지방산 조성을 분석한 결과는 다음과 같다. 난황유의 총지질과 중성지질의 지방산 조성은 oleic acid, palmitic acid, linoleic acid 순으로 주성분을 이루었고, 당지질은 palmitic acid, oleic acid, stearic acid, lauric acid 순으로, 인지질은 oleic acid, lauric acid, palmitic acid 순으로 주성분을 나타냈다. 포화지방산 함량은 방사구 보다 비방사구에서 약간 높았고, 포화지방산에 대한 불포화지방산의 비율(USFA/SFA)은 비방사구보다 방사구에서 높았다.

• 한국식품영양과학회지
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• 제14권3호
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• pp.296-300
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• 1985

1984년 6월 26일 경상남도 기장읍에서 구입한 미꾸라지의 분석결과는 다음과 같다. 1. 미꾸라지의 총질소중 단백태질소는 2189.1mg%, 유리아미노태질소는 408.1mg%, 휘발성염기질소는 46.3mg%를 차지하였다. 2. 미꾸라지의 구성아미노산조성은 glutamic acid, lysine, arginine, proline, aspartic acid, 등이 많이 함유되어 있었으며, 그 중 glutamic acid는 12.03%로 가장 높은 함량을 나타내었다. 3. 총 정제지질중 중성지질이 가장 많았고 인지질, 당지질순이었다. 중성지질의 지방산조성은 포화지방산과 monoene 산이 많았으며 주요지방산은 16:0, 16:1, 18:1 산이었다. polyene산에서는 18:2 산이 많았다. 당지질의 지방산조성은 중성지질과 비슷한 경향을 나타내고 있었으나 인지질에서는 중성지질이나 당지질보다 포화지방산과 polyene산이 많이 함유되어 있었다.

• Journal of Pharmaceutical Investigation
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• 제41권1호
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• pp.19-24
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• 2011

In order to enhance the clinical efficacy of 5-aminolevulinic acid-induced photodynamic therapy (ALA-PDT), liposomal formulations using bulk hydrogenated phospholipids from soybean were introduced. Three types of lipids, S75-3, S100-3, and SL80-3 were used for formulating ALA. The pH of all the liposomal ALA is 4.5~5.5 and the size is 50~200 nm. All the liposomal formulations gave better ex vivo ALA skin penetration using nude mice skin in Franz cell than free ALA did. Among them, SL80-3 including 22% of lyso-phosphocholine achieved excellent ALA penetration when compared with those of S75-3 and S100-3 which have only 1~2% of lyso-phospholipids. S100-3 showed a little better results than S75-3 did. Addition of humectants (glycerine, propylene glycol, butylene glycol, betaine) in liposomal ALA formulated with SL80-3 produced little enhancing effect in ALA penetration. On the other hand, addition of surfactants (Tween 20, 60, Brij 72, 76, 78) in same liposomal system produced significant increase in ALA penetration. Among them, transferosomal system of lyso-phospholipid, SL80-3 and the surfactant, Brij76 showed the highest ALA penetration. Furthermore, this system also established the highest in vivo PpIX biosynthesis in hairy mice skin of C57BL/6. These results concluded that the transferosome of SL80-3 and Brij76 produced the best results in both ALA penetration and PpIX biosynthesis, and proved good correlation between them.

• 생약학회지
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• 제27권4호
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• pp.397-407
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• 1996

This study was attempted to investigate the effect of Composite Preparation (Sam Whang Sasim-Tang: SWST, Whang Ryun Haedok-Tang: WRHT) on the activities of GOT and GPT, the content of total lipids, triglyceride, total cholesterol. Phospholipid and ${\beta}-lipoprotein$ in the serum, and the change ratio of body and liver weight in the experimentally induced hyperlipemic rats, making use of the extract 150, 200, 300 and 500 mg/kg p.o. Significant test was performed by comparision with the values of corresponding experimentally hyperlipemic rats. The activities of S-GPT was significantly decreased in all dose of SWST and WRHT respectively. The activities of S-GPT was significantly decreased in dose of SWST 500 mg/kg and in all dose of WRHT respectively. The content of total lipids, triglyceride, total cholesterol and phospholipids were significantly decreased in all dose of two the extract. The rate of decrease on total lipids and triglyceride were remarkable in dose of SWST 300, 500 mg/kg and WRHT 200, 500 mg/kg, and then the content of total cholesterol was more remarkable in dose SWST 300, 500 mg/kg, and WRHT 150, 300 mg/kg, and the content of phospholipids was more remarkable in dose of the extract 150, 200 and 300 mg/kg. The content of ${\beta}-lipoprotein$ was significantly decreased in dose of WRHT 150, 200 and in all dose of SWST. Increase ratio of the body and liver weight were significantly decreased in dose of two the extract 300, 500 mg/kg respectively.

• 한국응용과학기술학회지
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• 제3권2호
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• pp.35-40
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• 1986

자동산화유의 독성을 구명하기 위하여 autoxidized methyl linoleate(AOML)의 일정량을 mouse에 1 일 1 회 20 일간 경구 투여하여 혈청 및 간의 지질대사에 미치는 영향을 검토한 결과는 다음과 같다. 실험군의 혈청 TBA값과 혈청 triglyceride, phospholipid 및 total cholesterol양은 정상군에 비해 모두 증가되었으며, 전기영동 결과 혈청 lipoprotein의 조성 백분율은 albumin이 약 21% 증가된 반면, VLDL은 약 70% 그리고 LDL은 약 33% 감소되어 있었다. 실험군의 간에서의 POV, COV 및 TBA값은 모두 정상군에 비해 크게 증가하였으며, 또한 간의 triglyceride, phospholipid 및 total cholesteroldml 양도 증가하는 경향을 나타내고 있었다. 한편, 실험군의 간에서의 형태학적 변화로는 정상군에 비해 간세포의 손상과 지질의 축적이 관찰 되었다.

• Journal of Nutrition and Health
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• 제34권2호
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• pp.132-140
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• 2001

The effects of various dietary docosahexaenoix acid(DHA) levels on the brain phospholipids and serum and liver lipid compositions were studied in rats using DHA concentrated oil and corn oil as a control for 4 weeks. Serum total cholesterol and HDL cholesterol levels tended to be the lowest by adding 20% DHA to corn oil. Serum triglyceride levels significantly decreased by adding 30% DHA. Liver cholesterol and triglyceride levels were apparently decreased in the groups added above 20% DHA, especially, the lowest at adding 30% DHA. Brain weight and phospholipid content were not different among groups. The ratios of arachidonic to linoleic acids in serum and liver phosphatidylcholine(PC) were significantly decreased by adding dietary DHA and showed a flat form above 20% of dietary DHA. DHA levels of serum PC were gradually increased according to dietary DHA level. The fatty acid compositions of the brain PC and phosphatidylethanolamine(PE) did not appear any changes with accordance of the dietary DHA levels. However, compared with those of serum and liver in general, linoleic and arachidonic acid levels were very low. Oleic acids were apparently higher than those in the other tissues. DHA were higher than those in the other tissues rigardless of the dietary DHA, especially in brain PE. The ratios of arachidonic to linoleic acid were not apparent tendency in brain PC and PE. However, the ratios of brain PE were above 2 times higher than those of brain PC. As the results, the hypolipidemic effects of dietary DNA were remarkable in liver. Especially in regard to tendency of liver lipid levels and desaturation indices in serum and liver PC, the effects indicated significantly higher by adding 20-30% DHA to diet(n-6/n-3 ratio, about 4-7). Thus, in this study, these dietary DHA levels seemed to be appropriate, at least in these lipid paramenters.(Korean J Nutrition 34(2) : 132∼140, 2001)