• Title/Summary/Keyword: Phenotypic analysis

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Taxonomic Study on the Lichen Genus Xanthoparmelia (Ascomycotina, Parmeliaceae) in Korea

  • Wang, Xin Yu;Koh, Young-Jin;Hur, Jae-Seoun
    • Mycobiology
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    • v.36 no.4
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    • pp.203-210
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    • 2008
  • In previous studies investigating the genus Xanthoparmelia, thirteen different species have been reported from South Korea alone. However, there currently has been no revisional study performed until now. To explore the genus Xanthoparmelia, a phenotypic analysis was performed based on morphological, anatomical and chemical characters, while an investigation of Xanthoparmelia phylogeny was based on nuclear ribosomal (nr) DNA ITS sequences. A thorough examination of the specimens deposited in the Korean Lichen Research Institute (KoLRI) confirmed that eight species of Xanthoparmelia occur inside South Korea. Our analysis further confirmed the colors of the lower surface and medullar chemistry are important taxonomic characters in Xanthoparmelia. This study also presents a detailed description of each species and a key to the genus.

Quantitative Trait Loci Affecting Rous Sarcoma Virus Induced Tumor Regression Trait in F2 Intercross Chickens

  • Uemoto, Y.;Saburi, J.;Sato, S.;Odawara, S.;Ohtake, T.;Yamamoto, R.;Miyata, T.;Suzuki, K.;Yamashita, H.;Irina, C.;Plastow, G.;Mitsuhashi, T.;Kobayashi, E.
    • Asian-Australasian Journal of Animal Sciences
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    • v.22 no.10
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    • pp.1359-1365
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    • 2009
  • We performed a genome-wide linkage and quantitative trait locus (QTL) analysis to confirm the existence of QTL affecting Rous Sarcoma Virus (RSV) induced tumor regression, and to estimate their effects on phenotypic variance in an F2 resource population. The F2 population comprised 158 chickens obtained by crossing tumor regressive White Leghorn (WL) and tumor progressive Rhode Island Red (RIR) lines was measured for tumor formation after RSV inoculation. Forty-three tumor progressive and 28 tumor regressive chickens were then used for genome-wide linkage and QTL analysis using a total of 186 microsatellite markers. Microsatellite markers were mapped on 20 autosomal chromosomes. A significant QTL was detected with marker LEI0258 located within the MHC B region on chromosome 16. This QTL had the highest F ratio (9.8) and accounted for 20.1% of the phenotypic variation. Suggestive QTL were also detected on chromosomes 4, 7 and 10. The QTL on chromosome 4 were detected at the 1% chromosome-wide level explaining 17.5% of the phenotypic variation, and the QTLs on chromosome 7 and 10 were detected at the 5% chromosome-wide level and explained 11.1% and 10.5% of the phenotypic variation, respectively. These results indicate that the QTLs in the non-MHC regions play a significant role in RSV-induced tumor regression. The present study constitutes one of the first preliminary reports in domestic chickens for QTLs affecting RSV-induced tumor regression outside the MHC region.

Genotypic and Phenotypic Correlation and Path Coefficient Analysis in Barley (대맥 주요형질 간의 표현 및 유전상관과 경노계수)

  • 김흥배
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.30 no.2
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    • pp.174-179
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    • 1985
  • Estimate of heritabilities, genotypic and phenotypic correlations and path coefficient analysis were performed for the seven characters of barley cultivars (Hordeum vulgare L.). Heritabilities of broad sense for stem length and spike length were 0.923 and 0.907. Kernels per spike, grain yield and 100 grain weight also showed high heritabilities. High genotypic and phenotypic correlations existed between stem length and 100 grain weight. Grain yield showed highly significant phenotypic correlation with spike length, 100 grain weight and kernels per spike. Genotypic correlations between those characters were moderately high. Number of stem had greatest direct effect but the number of spike had a strong negative direct effect upon grain yield. Kernels per spike via number of spike showed greatest indirect effect and the number of stem via number of spike showed strong negative indirect effect upon grain yield.

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Phenotypic characterization of Hanwoo (native Korean cattle) cloned from somatic cells of a single adult

  • Yang, Byoung-Chul;Lee, Seung-Hwan;Hwang, Seong-Soo;Lee, Hwi-Cheul;Im, Gi-Sun;Kim, Dong-Hoon;Lee, Dong-Kyeong;Lee, Kyung-Tai;Jeon, Ik-Soo;Oh, Sung-Jong;Park, Soo-Bong
    • BMB Reports
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    • v.45 no.1
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    • pp.38-43
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    • 2012
  • We investigated phenotypic differences in Hanwoo cattle cloned from somatic cells of a single adult. Ten genetically identical Hanwoo were generated by somatic cell nuclear transfer from a single adult. Weights at birth, growing pattern, horn and noseprint patterns were characterized to investigate phenotypic differences. The weights of clones at 6 and 12 months were slightly heavier than that of the donor. A horn pattern analysis revealed that seven clones had exactly the same horn pattern as the donor cow, whereas three were different. Although similarities such as general appearance can often be used to identify individual cloned animals, no study has characterized noseprint patterns for this end. A noseprint pattern analysis of all surviving clones showed that all eight animals had distinct noseprints. Four were similar to the donor, and the remaining four had more secondary-like characteristics.

Genetic evaluation for economic traits of commercial Hanwoo population using single-step GBLUP

  • Gwang Hyeon Lee;Khaliunaa Tseveen;Yoon Seok Lee;Hong Sik Kong
    • Journal of Animal Reproduction and Biotechnology
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    • v.38 no.4
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    • pp.268-274
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    • 2023
  • Background: Recently, the single-step genomic best linear unbiased prediction (ssGBLUP) method, which incorporates not only genomic information but also phenotypic information of pedigree, is under study. In this study, we performed a ssGBLUP analysis on a commercial Hanwoo population using phenotypic, genotypic, and pedigree data. Methods: The test population comprised Hanwoo 1,740 heads raised in four regions of Korea, while the reference population used Hanwoo 18,499 heads raised across the country and two-generation pedigree data. Analysis was performed using genotype data generated by the Hanwoo 50 K SNP beadchip. Results: The mean Genome estimated breeding values (GEBVs) estimated using the ssGBLUP methods for carcass weight (CWT), eye muscle area (EMA), back fat thickness (BFT), and marbling score (MS) were 7.348, 1.515, -0.355, and 0.040, respectively, while the accuracy of each trait was 0.749, 0.733, 0.769, and 0.768, respectively. When the correlation analysis between the GEBVs as a result of this study and the actual slaughter performance was confirmed, CWT, EMA, BFT, and MS were reported to be 0.519, 0.435, 0.444, and 0.543, respectively. Conclusions: Our results suggest that the ssGBLUP method enables a more accurate evaluation because it conducts a genetic evaluation of an individual using not only genotype information but also phenotypic information of the pedigree. Individual evaluation using the ssGBLUP method is considered effective for enhancing the genetic ability of farms and enabling accurate and rapid improvements. It is considered that if more pedigree information of reference population is collected for analysis, genetic ability can be evaluated more accurately.

ESTIMATES OF PHENOTYPIC AND GENETIC PARAMETERS FOR WEANING AND YEARLING WEIGHTS IN BALI BEEF CATTLE

  • Djegho, Y.;Blair, H.T.;Garrick, D.J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.5 no.4
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    • pp.623-628
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    • 1992
  • Records on weaning (3803) and yearling weight (2990) of beef cattle (Bibos banteng) from the Bali Cattle Improvement Project were examined. A mixed model analysis involving all main non-genetic effects (village, year of birth, season of birth, age of dam, sex of calf, all significant interactions and age at weighing as a covariate) as fixed effects and sire nested within village as a random effect was undertaken. Variance components were estimated by Henderson's Method III. Paternal half-sib components of variance and covariance were used to estimate heritabilities of weaning and yearling weights, as well as their genetic and phenotypic correlations. Heritability estimates ($\pm$ standard error) obtained by Henderson's Method III for weaning and yearling weights were $.11{\pm}.03$ and $.13{\pm}.04$, respectively while the phenotypic and genetic correlations were estimated as .32 and $.64{\pm}.10$, respectively. The parameters estimated in this study were at the lower end of the range of reported values from various breeds. It is concluded that further information should be gathered to assist in estimating genetic parameters for other economic traits of Bali beef cattle and to provide more accurate estimates for weaning and yearling weights. These parameters should then be used to formulate a selection program to enable the genetic improvement of Bali Beef cattle.

Identification of functional SNPs in genes and their effects on plant phenotypes

  • Huq, Md. Amdadul;Akter, Shahina;Nou, Ill Sup;Kim, Hoy Taek;Jung, Yu Jin;Kang, Kwon Kyoo
    • Journal of Plant Biotechnology
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    • v.43 no.1
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    • pp.1-11
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    • 2016
  • Single nucleotide polymorphism (SNP) is an abundant form of genetic variation within individuals of species. DNA polymorphism can arise throughout the whole genome at different frequencies in different species. SNP may cause phenotypic diversity among individuals, such as individuals with different color of plants or fruits, fruit size, ripening, flowering time adaptation, quality of crops, grain yields, or tolerance to various abiotic and biotic factors. SNP may result in changes in amino acids in the exon of a gene (asynonymous). SNP can also be silent (present in coding region but synonymous). It may simply occur in the noncoding regions without having any effect. SNP may influence the promoter activity for gene expression and finally produce functional protein through transcription. Therefore, the identification of functional SNP in genes and analysis of their effects on phenotype may lead to better understanding of their impact on gene function for varietal improvement. In this mini-review, we focused on evidences revealing the role of functional SNPs in genes and their phenotypic effects for the purpose of crop improvements.

Identification of SNPs Related to 19 Phenotypic Traits Using Genome-wide Association Study (GWAS) Approach in Korean Wheat Mini-core Collection

  • Yuna Kang;Yeonjun Sung;Seonghyeon Kim;Changsoo Kim
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2020.06a
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    • pp.120-120
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    • 2020
  • Based on the simple sequence repeat (SSR) marker, a Korean wheat core collection were established with 616 wheat accessions. Among them, the SNP genotyping for the entire genome was performed using DNA chip array to clarify the whole genome SNP profiles. Consequently, a total of 35,143 SNPs were found and we re-established a mini-core collection with 247 accessions. Population diversity and phylogenetic analysis revealed genetic diversity and relationships from the mini core set. In addition, genome-wide association study (GWAS) was performed on 19 phenotypic traits; ear type, awn length, culm length, ear length, awn color, seed coat color, culm color, ear color, loading, leaf length, leaf width, seeding stand, cold damage, weight, auricle, plant type, heading stage, maturation period, upright habit, and degree of flag leaf. The GWAS was performed using the fixed and random model circulating probability unification (FarmCPU), which identified 14 to 258 SNP loci related to 19 phenotypic traits. Our study indicates that this Korean wheat mini-core collection is a set of germplasm useful for basic and applied research with the aim of understanding and exploiting the genetic diversity of Korean wheat varieties.

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Phenotypic and Genotypic Correlations between Some Characters of Mulberry Trees (상수 각형질 상호간의 표현형상관과 유전상관)

  • 장권열;한경수;민병열
    • Journal of Sericultural and Entomological Science
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    • no.12
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    • pp.1-4
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    • 1970
  • The experimental studies were intended to clarify the effects of leaf yield calculations, and also aimed at estimating the correlations between some characters for the selection of desirable stocks of mulberry trees. The analysis of covariances was the variance components procedures in a replicated trial for the varieties, and also it was used to obtain the genotypic, phenotypic and environmental correlations between the eight characters-branch length, node number, branch diameter, branch number per stock, total branch weight, old branch weight, new shoot and leaf weight, and leaf weight, etc. The results obtained are summarized as follows: Phenotypic, genotypic and environmental covariances are shown in Table 1 and phenotypic correlations, genotypic correlations and environmental correlations are shown in Table 2. Genotypic correlations were slightly higher than the corresponding phenotypic correlations between the characters in the varieties, but the variation in values due to the change of environment appeared between the characters. Genotypic correlations between the leaf yield of mulberry trees and other characters indicated that high leaf yield was genetically correlated with six characters namely branch length, node number, branch number per stock, total branch weight, old branch weight and new shoot weight, etc. It was also observed that lower correlations were appeared between the leaf yield and branch diameter, and branch diameter and other all characters studied in both genotypic and phenotypic correlation coefficients.

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Genetic Evaluation of First Lactation Traits in Sahiwal Cattle Using Restricted Maximum Likelihood Technique

  • Choudhary, V.;Kothekar, M.D.;Raheja, K.L.;Kasturiwale, N.N.;Khire, D.W.;Kumar, P.
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.5
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    • pp.639-643
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    • 2003
  • The data on 283 Sahiwal cows, sired by 16 bulls, maintained at Cattle Breeding Farm of Nagpur Veterinary College and Dairy Farm of Agricultural College, Nagpur, were considered for the estimation of genetic parameters. Variance and covariance estimates of first lactation traits were obtained using restricted maximum likelihood technique (REML). When first lactation milk yield (FLMY), first lactation length (FLL) and average daily yield (ADY) traits were considered for REML analysis, the heritabilities were $0.184{\pm}0.146$, $0.132{\pm}0.131$ and $0.141{\pm}0.133$, respectively. While, genetic and phenotypic correlations between them were medium to high except phenotypic correlations between FLL and ADY (-0.025). REML procedure considering FLMY, age at first calving (AFC) and first service period (FSP) combination exhibits heritabilities as $0.274{\pm}0.173$, $0.506{\pm}0.233$ and $0.274{\pm}0.172$, respectively. Genetic correlations were $-0.120{\pm}0.376$, $0.225{\pm}0.423$ and $0.365{\pm}0.331$ between FLMY and AFC, FLMY and FSP, AFC and FSP, respectively. Phenotypic correlations were 0.057, 0.289 and 0.123, respectively. Considering all five traits REML combination heritabilities estimated were $0.238{\pm}0.162$, $0.160{\pm}0.139$, $0.136{\pm}0.132$, $0.409{\pm}0.209$ and $0.259{\pm}0.168$ for FLMY, FLL, ADY, AFC and FSP, respectively. The genetic correlations were positive except FLMY and AFC. The phenotypic correlations were also positive except FLL and ADY, ADY and FSP. Almost all estimates were associated with high standard error.