• Journal of Ocean Engineering and Technology
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• v.37 no.6
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• pp.238-246
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• 2023

This paper proposes an effective inertia coefficient (EIC) in the Morison equation for better wave-force calculations. The OC4 semi-submersible floating offshore wind turbine (FOWT) platform was considered to test the feasibility. Large diffraction at large Keulegan-Carpenter (KC) numbers and the interaction between columns can result in errors in estimating the wave force using the Morison equation with a theoretical inertia coefficient, which can be corrected by the EIC as a function of the wave period and direction. The horizontal and vertical wave forces were calculated using the Morison equation and potential theory at each column, wave period, and wave direction. The EICs of each column were then obtained, resulting in a minimal difference between the Morison inertia force and the wave excitation force by the potential theory. The EICs, wave forces, phase angles, and dynamic motions were compared to confirm the feasibility of an EIC concept under regular and random waves.

• Journal of Yeungnam Medical Science
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• v.41 no.2
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• pp.61-73
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• 2024

Acute kidney ischemia-reperfusion (IR) injury is a life-threatening condition that predisposes individuals to chronic kidney disease. Since the kidney is one of the most energy-demanding organs in the human body and mitochondria are the powerhouse of cells, mitochondrial dysfunction plays a central role in the pathogenesis of IR-induced acute kidney injury. Mitochondrial dysfunction causes a reduction in adenosine triphosphate production, loss of mitochondrial dynamics (represented by persistent fragmentation), and impaired mitophagy. Furthermore, the pathological accumulation of succinate resulting from fumarate reduction under oxygen deprivation (ischemia) in the reverse flux of the Krebs cycle can eventually lead to a burst of reactive oxygen species driven by reverse electron transfer during the reperfusion phase. Accumulating evidence indicates that improving mitochondrial function, biogenesis, and dynamics, and normalizing metabolic reprogramming within the mitochondria have the potential to preserve kidney function during IR injury and prevent progression to chronic kidney disease. In this review, we summarize recent advances in understanding the detrimental role of metabolic reprogramming and mitochondrial dysfunction in IR injury and explore potential therapeutic strategies for treating kidney IR injury.

• Nuclear Engineering and Technology
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• v.56 no.3
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• pp.873-879
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• 2024

A sodium-cooled fast reactor (SFR) core has a potential of prompt criticality due to a change of core material distribution during a severe accident, and the resultant energy release has been one of the safety issues of SFRs. In this study, the safety assessment of an unprotected loss-of-flow (ULOF) in a small SFR (SSFR) has been performed using the SIMMER-IV computer code, which couples the models of space- and time-dependent neutronics and multi-component, multi-field thermal hydraulics in three dimensions. The code, therefore, is applicable to the simulations of transient behaviors of extended disrupted core material motion and its reactivity effects during the transition phase (TP) of ULOF, including a potential of prompt-criticality power excursions driven by fuel compaction. Several conservative assumptions are used in the TP analysis by SIMMER-IV. It was found out that one of the important mechanisms that drives the reactivity-inserting fuel motion was sodium vapor pressure resulted from a fuel-coolant interaction (FCI), which itself was non-energetic local phenomenon. The uncertainties relating to FCI is also evaluated in much conservative way in the sensitivity analysis. From this study, the ULOF characteristics in an SSFR have been understood. Occurrence of recriticality events under conservative assumptions are plausible, but their energy releases are limited.

• Biomolecules & Therapeutics
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• v.32 no.5
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• pp.640-646
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• 2024

Hair growth cycles are mainly regulated by human dermal papilla cells (hDPCs) and human outer root sheath cells (hORSCs). Protecting hDPCs from excessive oxidative stress and hORSCs from glycogen phosphorylase (PYGL) is crucial to maintaining the hair growth phase, anagen. In this study, we developed a new PYGL inhibitor, hydroxytrimethylpyridinyl methylindolecarboxamide (HTPI) and assessed its potential to prevent hair loss. HTPI reduced oxidative damage, preventing cell death and restored decreased level of anagen marker ALP and its related genes induced by hydrogen peroxide in hDPCs. Moreover, HTPI inhibited glycogen degradation and induced cell survival under glucose starvation in hORSCs. In ex-vivo culture, HTPI significantly enhanced hair growth compared to the control with minoxidil showing comparable results. Overall, these findings suggest that HTPI has significant potential as a therapeutic agent for the prevention and treatment of hair loss.

• International conference on construction engineering and project management
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• 2024.07a
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• pp.431-438
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• 2024

Monitoring unsafe activities on construction sites is challenging due to a variety of factors including the diversity of tasks and workers involved, the potential of human error and lack of real-time hazard detection. With technological advancements, several digital technologies have been proposed and applied to improve the monitoring process. Despite the potential of these technological advancements to reduce manual effort in traditional monitoring, the challenge lies in selecting and implementing the technology that best meets the specific needs of contractors. This paper aims to streamline the research of digital technologies in the construction domain by achieving three key objectives: (1) classify the types of unsafe activities that can be monitored automatically, (2) determine the specific data required for effective monitoring processes, and (3) identify the technologies that can facilitate such data collection process. We conduct a systematic literature review on cutting-edge technological studies to achieve the research aims. The findings of this research serve as a valuable resource for construction practitioners, offering insights into both the benefits and limitations of digital technologies in enhancing the monitoring process. Moreover, the study recommends preparatory elements that practitioners should undertake to integrate these technologies effectively into their monitoring frameworks. The study empowers practitioners by providing a deep understanding, enabling them to create a comprehensive safety management program aligned with the digital transformation process.

• Microbiology and Biotechnology Letters
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• v.52 no.3
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• pp.314-324
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• 2024

Due to the hazardous effects of synthetic pigments, natural pigments are gaining popularity. Among natural sources microorganisms have become a major source of numerous industrially essential items and their use for getting various natural products have expanded dramatically in recent years. In the present study, 9 endophytic fungal strains were isolated from Tagetes erecta. On screening, yeast strain MGI was selected for further study which was identified as Rhodotorula mucilaginosa MGI. The pigment was intracellular, and the color of the crude extract was orange. The extract was subjected to characterization by UV-visible spectrophotometer and was purified by column phase chromatography, after purification two pigmented fractions were obtained. These fractions on characterization by thin layer chromatography (TLC) and Fourier transform infrared (FTIR) spectrophotometer affirms that they belong to carotenoid group of pigments. Orange (F1) and yellow (F2) fractions were anticipated as astaxanthin and beta carotene respectively. Moreover, the bioactive potential of pigmented fractions was investigated which manifested that F1 exhibited a maximum antioxidant activity of about 77% and F2 showed the highest zone of inhibition of 38 mm against Staphylococcus aureus. Thus, this study reflects that an endophytic yeast strain has the potential for the production of bioactive microbial pigments.

• Journal of Korean Society of Environmental Engineers
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• v.22 no.2
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• pp.193-201
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• 2000

Partitioning of hydrophobic organic compounds (HOCs) to a biosurfactant, hydroxypropyl-${\beta}$-cyclodextrin (HPCD), was conducted to evaluate the feasibility of using HPCD to remove HOCs from soil/groundwater. HOC partitioning to HPCD was very fast, with over 95% of the complexation occurring within 10 min. Some influence of solution chemistry and HOC concentration on HOC-HPCD complex formation coefficients was observed. HPCD sorption on soil as quantified by both a fluorescence technique and total organic carbon measurements was negligible, indicating no significant affinity of HPCD for the solid phase. Although the HOC solubilization capability of HPCD was lower than that of synthetic surfactants such as SDS and Tween 80, HPCD can be effective in removing sorbed HOCs from a model subsurface environment, primarily because of its negligible sorption to the solid phase (i.e., all the HPCD added facilitates HOC elution). However, in contrast with conventional surfactants, HPCD becomes relatively less effective for HOC partitioning with increasing HOC size and hydrophobicity. Therefore, comparisons between HPCD and synthetic surfactants for enhanced remediation applications must consider the specific HOC(s) present and the potential for surfactant material losses to the solid phase, as well as other more generally recognized considerations such as material costs and potential toxicological effects.

• Journal of the Korean Ceramic Society
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• v.24 no.3
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• pp.243-250
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• 1987

The effects aging on some properties and thermal-shock behavior of zirconia partially stabilized with 9 mol% MgO (9MZ) were studied. 9MZ specimens were aged over $1200^{\circ}$-$1400^{\circ}C$ for 12hours subsequently, after sintering at $1650^{\circ}C$ for 4 hours. Fracture strength(both before and after thermal-shock test), linear thermal expansion, monoclinic fraction and phase transition by XRD, density, galvanic potential and microstructure were measured. Quantitative chemical analysis around the grain-boundary of the specimen aged at $1350^{\circ}C$ was also conducted by EDX. The aging of 9MZ specimen causes a thermal decomposition of cubic-$ZrO^{2}$ into the formation metastable tetragonal-$ZrO^{2}$ and MgO. The former increases the residual strength after thermal-shock test and the latter improves the thermal-shock resistance due to thermal conduction through the continuous magnesia phase and the formation of monoclinic phase content in matrix were increased with decreasing the aging temperature from $1400^{\circ}C$ to $1200^{\circ}C$. Galvanic potential of the aged specimen exhibited a proper emf characteristic.

• Korean Journal of Head & Neck Oncology
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• v.22 no.2
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• pp.130-136
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• 2006

Introduction : The sensitivity of tumor cells to radiotherapy is a critical determinant of local control and potential cure in advanced head and neck squamous cell carcinoma(HNSCC). The emergence of radioresistant tumor cells is an obstacle to cancer therapy. Most radioresistant cells have a higher proportion of cells in the Sphase of the cell cycle and a lower apoptotic fraction than radiosensitive cells. HSV replication is increased in cells that have higher S-phase fractions. NV1066 is an oncolytic herpes simplex virus type-1 mutant. We hypothesized that NV1066 replication and cytotoxicity are increased in radioresistant cells. The purpose of this study is to evaluate the antitumor efficacy of NV1066 to treat radioresistant HNSCC. Methods : Radioresistant cells were selected by treating five HNSCC cell lines with repeated conventional fractionated doses of radiation(2Gy/day), using a Cs-137 irradiator, up to a cumulative dose of 70Gy. Clonogenic cell survival and S-phase fractions were compared between radioresistant and parental radiosensitive cells. The two cell populations were then treated with NV1066 to examine viral replication, by the viral plaque assay and viral cytotoxicity. Results : Fractionated irradiation resulted in the selection of radioresistant cells. Radioresistant cells had a higher S-phase fraction(42.9%) compared to parental cells(26.2%). NV1066 replication in radioresistant cells was 7.4 times higher than in parental cells(p<0.01). Treatment with NV1066 resulted in increased cytotoxicity of 24.5% in radioresistant cells compared to parental cells(p<0.05). Conclusion : NV1066 showed increased viral replication and cytotoxicity in radioresistant HNSCC cell lines. These findings suggest a potential clinical application for this oncolytic viral therapy as treatment for radioresistant head and neck cancers.

• Journal of Embryo Transfer
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• v.10 no.1
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• pp.73-82
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• 1995

large scale production of cloned embryos requires the technology of multiple generation nuclear transplantation(NT) using NT embryos as the subsequent donor nuclei. The purposes of this study were producing the second generation cloned rabbit embryos, and also to determine the electrofusion rate and in vitro developmental potential comparatively in the cloned embryos of the first and second NT generation. The embryos of 16-cell stage were collected from the mated does by flushing oviducts with Dulbecco's phosphate buffered saline(D-PBS) containing 10% fetal calf serum(FCS) at 47 hours after hCG injection In the first generation NT, the nuclear donor embryos were synchronized in the phase of Gi /S transition of 32-cell stage. The first generation NT embryos which were developed to 8-cell were synchronized in Gi /S transition phase of the following 16-cell stage and used as donor nuclei for second generation Synchronization of the cell cycle of blastomeres was induced, first, using an inhibitor of microtuble polymerization, colcemid for 10 hours to arrest blastomeres in M phase, and secondly, using a DNA synthesis inhibitor, aphidicolin for 1.5 to 2 hours to arrest them in Gi /S transition boundary. The recipient cytoplasms were obtained by removing the nucleus and the first polar body from the oocytes collected at 14 hours after hCG injection. The separated donor blastomeres were injected into the enucleated recipient oocytes by micromanipulation and were electrofused by electrical stimulation of three pulses for 60 $\mu$sec at 1.25 kV /cm in 0.28 M rnannitol solution The fused oocytes were co-cultured with a monolayer of rabbit oviductal epithelial cells in M-199 solution containing 10% FCS for 120 hours at 39$^{\circ}C$ in a 5% $CO_2$ incubator. Following in vitro culture of the first and second generation cloned embryos to blastocyst stage, they were stained with Hoechst 33342 dye for counting the number of blastomeres by fluorescence microscopy. The results obtained were summarized as follows: 1. The electrofusion rate was found to be similar as 79.4 and 91.5% in the first and second generation NT rabbit embryos, respectively. 2. The in vitro developmental potential to blastocyst stage of the second generation NT embryos (23.3%) was found significantly(p<0.05) lower, compared with that of the first generation NT embryos (56.8%). 3. The mean blastomeres counts of embryos developed to blastosyst stage following in vitro culture for 120 hours and also their daily cell cycles during the culture period were decreased significantly (p<0.05) to 104.3 cells and 1.33 cylces in the second NT generation, compoared with 210.4 cells and 1.54 cycles in the first NT generation, respectively.