• Asian Pacific Journal of Cancer Prevention
• /
• v.15 no.16
• /
• pp.6977-6982
• /
• 2014

Background: The aim of this study was to investigate the effect of a Lipofectamine2000 (Life2000) Transfection Reagent transfected psiRNA-STAT3 plasmid on 4T1 breast cancer cells. Materials and Methods: MTT was used to detect the cell proliferation of breast cancer 4T1 cells at different periods (0h, 6h, 8h, 10h); the cell cycle was assessed by flow cytometry; variation of apoptosis and mitochondrial membrane potential was observed under a fluorescence microscope; immunohistochemical staining was used to determine the expression of caspase-3 and cyclin-D1 protein. Results: An obvious effect of inhibition to 4T1 cancer cells could be observed at 8h after the psiRNA-STAT3 was transfected. Typical alterations of apoptotic morphological features were visible in the psiRNA-STAT3 treatment group. Mitochondrial membrane potential decreased significantly, the number of cells was increased in G0/G1 phase, and the number of cells was decreased in S phase, and the data were statistically significant (p<0.05), compared with the Scramble and Mock groups. Expression of caspase-3 protein was increased significantly, while that of cyclin D1 was significantly decreased. Conclusions: Life2000 transfected psiRNA-STAT3 plasmid can inhibit 4T1 tumor cell proliferation and promote apoptosis of 4T1 tumor cells, which process depends on the regulation of expression of cyclin D1 and caspase-3 protein.

• Asian Pacific Journal of Cancer Prevention
• /
• v.15 no.18
• /
• pp.7527-7532
• /
• 2014

Saussurea involucrata is a Mongolian medicinal plant well known for its effects in promoting blood circulation, and anti-inflammation and analgesic functions. Earlier studies reported that Saussurea involucrata has anticancer activity. The purpose of this study was to confirm the anticancer activity of an ethanol extract of Saussurea involucrata against hepatic cancer and elucidate its mechanisms of action. Hepatocellular carcinoma cells were tested in vitro for cytotoxicity, AO/EB staining for apoptotic cells, apoptotic DNA fragmentation and cell cycle distribution in response to Saussurea involucrata extract (SIE). The mRNA expression of caspase-3,-9 and Cdk2 and protein expression of caspase-3,-9, PARP, XIAP, Cdk2 and p21 were analyzed through real time PCR and Western blotting. Treatment with SIE inhibited HepG2 cell proliferation dose- and time-dependently, but SIE only exerted a modest cytotoxic effect on a viability of Chang human liver cells. Cells exposed to SIE showed typical hallmarks of apoptotic cell death. Cell cycle analysis revealed that SIE caused G1-phase arrest in HepG2 cells. In conclusion, Saussurea involucrata ethanol extract has potential cytotoxic and apoptotic effects on human hepatocellular carcinoma cells. Its mechanism of action might be associated with the inhibition of DNA synthesis, cell cycle (G1) arrest and apoptosis induction through up-regulation of the protein expressions of caspase-3,-9 a nd p21, degradation of PARP and down-regulation of the protein expression of Cdk2 and XIAP.

• Spatial Information Research
• /
• v.22 no.1
• /
• pp.27-33
• /
• 2014

Considering the wide coverage, the transparency from climate condition, Interferometric Synthetic Aperture Radar (InSAR) possesses a great potential for the landcover classification as shown in many precedent researches. In addition to the merits of InSAR products for the landcover classification, the time series analysis of InSAR pairs can provide a highly reliable basis to interpret landcover. We applied such idea with the test site in Mountain Baekdu located on the border between North Korea and China. Since it is recently noted as the potential volcanic activation site, the landcover especially the vegetation distribution information is highly essential to validate the reliability of Differential Interferometric Synthetic Aperture Radar (DInSAR) over Mt. Baekdu. The algorithms combining the auxiliary information from Moderate Resolution Imaging Spectroradiometer (MODIS) to analyze the phase coherence and backscatter coefficient of Observing Satellite (ALOS) Phased Array type L-band Synthetic Aperture Radar (PALSAR) was established. The results using InSAR signatures from two polarization modes of ALOS PALSAR showed high reliability for mining landcover and spatial distribution.

• Asian-Australasian Journal of Animal Sciences
• /
• v.29 no.1
• /
• pp.16-22
• /
• 2016

The intestinal environment plays a critical role in maintaining swine health. Many factors such as diet, microbiota, and host intestinal immune response influence the intestinal environment. Intestinal alkaline phosphatase (IAP) is an important apical brush border enzyme that is influenced by these factors. IAP dephosphorylates bacterial lipopolysaccharides (LPS), unmethylated cytosine-guanosine dinucleotides, and flagellin, reducing bacterial toxicity and consequently regulating toll-like receptors (TLRs) activation and inflammation. It also desphosphorylates extracellular nucleotides such as uridine diphosphate and adenosine triphosphate, consequently reducing inflammation, modulating, and preserving the homeostasis of the intestinal microbiota. The apical localization of IAP on the epithelial surface reveals its role on LPS (from luminal bacteria) detoxification. As the expression of IAP is reported to be downregulated in piglets at weaning, LPS from commensal and pathogenic gram-negative bacteria could increase inflammatory processes by TLR-4 activation, increasing diarrhea events during this phase. Although some studies had reported potential IAP roles to promote gut health, investigations about exogenous IAP effects or feed additives modulating IAP expression and activity yet are necessary. However, we discussed in this paper that the critical assessment reported can suggest that exogenous IAP or feed additives that could increase its expression could show beneficial effects to reduce diarrhea events during the post weaning phase. Therefore, the main goals of this review are to discuss IAP's role in intestinal inflammatory processes and present feed additives used as growth promoters that may modulate IAP expression and activity to promote gut health in piglets.

• Asian Pacific Journal of Cancer Prevention
• /
• v.14 no.5
• /
• pp.2973-2978
• /
• 2013

Maesil (Prunus mume Siebold & Zucc.), a member of the genus Rosaceae, has been reported to have antioxidative effects, as well as anticancer influence in many cancer lines. Thus, this present study was designed to investigate the inhibitory effect of fermented Maesil with probiotics against 7,12-dimethylbenz[a]anthracene (DMBA), 12-O-tetradecanoyl phorbol 13-acetate (TPA)-induced mouse skin carcinogenesis via its antioxidative potential. Mice were fed a diet containing fermented Maesil, containing either 1% (1% FM fed group) or 2% (2% FM fed group) along with probiotics following DMBA and TPA exposure. Continuous ingestion of the experimental feed markedly inhibited skin carcinogenesis, as evidenced by a marked decrease in papilloma numbers and epidermal hyperplasia as well as cellular proliferation and the percentage of proliferating-cell nuclear antigen positive cells. Also, the FM fed group showed an increase of total antioxidant capacity as well as an increased level of phase II detoxifying enzymes such as superoxide dismutase, concurrent with a decreased lipid peroxidation activity level. Taken together, these results suggest that fermented Maesil has the ability to suppress the development of DMBA-TPA induced skin carcinogenesis, via the reduction of lipid peroxidation, enhancing total antioxidant capacity and phase II detoxifying enzyme.

• Asian Pacific Journal of Cancer Prevention
• /
• v.14 no.2
• /
• pp.951-957
• /
• 2013

Our aim was to investigate the chemopreventive potential of saffron in DMBA-induced hamster buccal pouch carcinogenesis. Assessment was by monitoring the percentage of tumor bearing hamsters, tumor size as well as the status of detoxification agents, lipid peroxidation and antioxidants. Oral squamous cell carcinomas were induced in the buccal pouch of Syrian golden hamsters by painting them with 0.5% DMBA in liquid paraffin three times a week for 14 weeks. We observed 100% oral tumor formation with severe histopathological abnormalities in all the hamsters treated with DMBA alone, activities of phase I and phase II detoxification enzymes, lipid peroxidation and antioxidants being significantly altered. Though oral administration of saffron completely prevented the formation of tumors, we noticed severe hyperplasia and dysplasia in hamsters treated with DMBA, suggesting that tumors might eventually develop. Oral administration of saffron return detoxification enzymes, lipid peroxidation and antioxidants to normal ranges. The chemopreventive potential of saffron thus is likely due to antioxidant properties and modulating effects on detoxification in favour of the excretion of carcinogenic metabolites during DMBA-induced hamster buccal pouch carcinogenesis.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.20 no.12
• /
• pp.475-481
• /
• 2019

The importance of performance-based design feedback is being emphasized when it comes to the potential impact that affects all the lifecycle of the building. However, the latency and disconnection of domain expert in the sector of AEC/FM remain current obstacles between design and performance feedback. It is hard to utilize performance feedback information for design exploration and support design decision making during the conceptual phase of design. Using parametric design, this paper proposes various design alternatives from a set of rules and constraints defined by algorithms for the geometric configurations of an Office Building. A Building Performance Analysis (BPA) was to developed using Autodesk® Revit® 2019 which integrates Autodesk® Green Building Studio® to predict its sufficient daylighting conditions of the LEED v4's Daylighting Autonomy (DA). The parametric-based performance feedback of this study outlines potential design improvements for further exploration in application to the early design process.

• Journal of Korean Ophthalmic Optics Society
• /
• v.7 no.1
• /
• pp.51-55
• /
• 2002

This study was to investigate the effect of EEG wave type of visual cortex on conjugate movement of eyeball according to movement of visual target. Visual evoked potential(VEP) system used the Bio-Pag(production in USA) and recorded to 586 computer. The illumination of test room was 50lux and the visual target was red light dot of 3cm size. The results of dextroversion and levoversion as follows : The visual stimulation waves on the visual cortex have about 71% of delta wave, about 12% of beta wave, about 9% theta wave and about 6% of alpha wave respectively. The dextroversion and levoversion state was similar results on the histogram amplitude of EEG wave, frequency of EEG wave type, EEG wave style and phase diagram of amplitude. Expecially the histogram amplitude of EEG wave appeared almost the Gaussian shape and the phase analysis of amplitude of EEG wave was nearly linear shape. On the fast fourier transform of the amplitude and Hz, the frequency was almost low frequency under 20 Hz, and the dextroversion and levoversion shape was similar results.

• Smart Media Journal
• /
• v.5 no.3
• /
• pp.35-41
• /
• 2016

In this paper, we propose hand-gesture signal recognition based on EPS(Electronic Potential Sensor) using Deep learning model. Extracted signals which from Electronic field based sensor, EPS have much of the noise, so it must remove in pre-processing. After the noise are removed with filter using frequency feature, the signals are reconstructed with dimensional transformation to overcome limit which have just one-dimension feature with voltage value for using convolution operation. Then, the reconstructed signal data is finally classified and recognized using multiple learning layers model based on deep learning. Since the statistical model based on probability is sensitive to initial parameters, the result can change after training in modeling phase. Deep learning model can overcome this problem because of several layers in training phase. In experiment, we used two different deep learning structures, Convolutional neural networks and Recurrent Neural Network and compared with statistical model algorithm with four kinds of gestures. The recognition result of method using convolutional neural network is better than other algorithms in EPS gesture signal recognition.

• KIPS Transactions on Computer and Communication Systems
• /
• v.3 no.12
• /
• pp.463-478
• /
• 2014

Portal site is not only providing search engine and e-mail service but also various services including blog, news, shopping, and others. The fact that average number of daily login for Korean portal site Naver is reaching 300 million suggests that many people are using portal sites. With the increase in number of users followed by the diversity in types of services provided by portal sites, the attack is also increasing. Most of studies of password authentication is focused on threat and countermeasures, however, in this study, we analyse the security threats and security requirement of membership, login, password reset first phase, password reset second phase. Also, we measure security score with common criteria of attack potential. As a result, we compare password authentication system of domestic and abroad portal sites.