• Journal of Embryo Transfer
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• v.12 no.1
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• pp.11-20
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• 1997

The influence of cryopreservation of donor embryos on the in vitro developmental potential in the nuclear transplant rabbit embryos was evaluated. The embryos of 16-cell stage were collected and cryopreserved with EFS solution by vitrification method. The frozen embryos were thawed and synchronized to S and G$_1$ phase of 32-cell stage. The recipient/ cytoplasms were obtained by removing the first polar body and chromosome mass from the oocytes collected by non-disruptive microsurgery procedure. The separated S and G$_1$ phase blastomeres of 32-cell stage were injected into enucleated recipient cytoplasms by micromanipulation. After culture until 20 hrs post-hCG injection, the nuclear transplant oocytes were electrofused and activated by electrical stimulation. The fused nuclear transplant embryos were co-cultured with rabbit oviduct epithelial cells. After in vitro culture for 120 hrs, the nuclear transplant embryos developed to blastocyst stage were stained with Hoechst 33342 dye and their blastomeres were counted. The electrofusion rate was significantly (P<0.05) reduced in the frozen nuclear donor,compared with fresh donor nuclei as 80.0 vs 62.8% in S phase and 81.7 vs 64.8% in G$_1$phase, respectivley. The in vitro developmental rate to blastocyst stage with the S and G$_1$phase of fresh embryos(26.3 and 61.1%, respectively) was found significantly (P<0.05) higher, compared to the S and G]phase of frozen embryos(11.9 and 34.6%, respectively). When frozen as well as fresh donor embryos were synchronized to G$_1$ phase, the in vitro developmental rate to blastocyst stage was significantly (P<0.05) higher, compared with S phase donor nuclei. The cell counts of nuclear transplant embryos developed to blastosyst stage were significantly (P<0.05) more in G$_1$ phase of fresh or frozen embryos (180.1 and 125.7 cells, respectively), compared with S phase nuclear donor (145.1 and 103.7 cells, respectively). From the above results it was concluded that the rabbit embryos cryo- preserved by vitrification might be available as nuclear donor, though the developmentalpotential and cell counts of nuclear transplant rabbit embryos were decreased significantly.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.14 no.11
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• pp.4355-4371
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• 2020

Phase retrieval, recovering a signal from phaseless measurements, is generally considered to be an NP-hard problem. This paper adopts an amplitude-based nonconvex optimization cost function to develop a new stochastic gradient algorithm, named stochastic reweighted phase retrieval (SRPR). SRPR is a stochastic gradient iteration algorithm, which runs in two stages: First, we use a truncated sample stochastic variance reduction algorithm to initialize the objective function. The second stage is the gradient refinement stage, which uses continuous updating of the amplitude-based stochastic weighted gradient algorithm to improve the initial estimate. Because of the stochastic method, each iteration of the two stages of SRPR involves only one equation. Therefore, SRPR is simple, scalable, and fast. Compared with the state-of-the-art phase retrieval algorithm, simulation results show that SRPR has a faster convergence speed and fewer magnitude-only measurements required to reconstruct the signal, under the real- or complex- cases.

• Proceedings of the KSR Conference
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• 2008.11b
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• pp.23-29
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• 2008

Most VE operation related to construction project is progressed in stage of planing or design phase, especially in case of domestic. It is encouraging fact and if you have VE process in stage of planning and design, you'll have experience a reduction of about 70% of total saving cost in which total construction process may occurred. However, recently the work of construction in overseas increase and almost is only construction-work, except planning and design process. As this reason, VE process for construction-work phase only is demanded in high necessity. VE in construction phase comes to accomplish intensively in the part which the design change does not follow, through know-how, equipment operating method and method of temporary-structure erection at the established construction-site learned, builders will be the possibility of reduction from 3% to 10% in constructive expense. This paper describes VE performing example in OO company's construction-work phase, and propose the methodology of VE performance in the phase of construction. And also it is aimed to extending VE applications into the overseas construction enterprise.

• Journal of KIBIM
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• v.5 no.4
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• pp.1-10
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• 2015

In order to apply the BIM technology effectively and efficiently during the life-cycle of project, it is important to deliver a accurate information on BIM from designer, and it should be used effectively at construction or maintenance stage. However, stakeholders have created a new BIM model at each phase in many practise adopting BIM, not used BIM generated in design stage. Because they have different purposes for using BIM, and deliver the BIM model without the sufficient explanation or application. It is necessary to define the range of usable BIM in construction phase with analyzing clearly the BIM created in design phase, and determining the difference with required BIM in construction phase. Therefore, this study propose a method for construction c

• Journal of the Korea Institute of Information and Communication Engineering
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• v.9 no.3
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• pp.638-646
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• 2005

In recent wireless communications, direct conversion transceiver or If sampling SDR-based receivers have being designed as an alternative to conventional transceiver topologies. In direct conversion receiver a.chitectu.e, the 1.equency/phase offset between the RF input signal and the local oscillator signal is a major impairment factor even though the conventional AFC/APC compensates the service deterioration due to the offset. To rover the limited tracking range of the conventional method and effectively aid compensation scheme in terms of I/Q channel imbalances, the frequency/phase offset compensation in RF-front end signal stage is proposed in this paper. In RF-front end, the varying phase offset besides the fixed large frequency/phase offset are corrected by using early-late phase compensator. A more simple frequency and phase tacking function in digital signal processing stage of direct conversion receiver is effectively available by an ingenious frequency/phase offset tracking method in RF front-end stage.

• Macromolecular Research
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• v.9 no.1
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• pp.51-65
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• 2001

Two-stage emulsion polymerizations of hydrophobic monomers on hydrophilic seed polymer particles were carried out to make core-shell composite particles. It was found that the loci of polymerization in the second stage were the surface layer of the hydrophilic seed latex particles, and that it has resulted in the formation of either eccentric core-shell particles with the core exposed to the aqueous phase or aggregated nonspherical composite particles with the shell attached on the seed surface as many small separated particles. The driving force of these phenomena is related to the gain in free energy of the system in going from the hydrophobic polymer-water interface to hydrophilic polymer-water interface. Thermodynamic analysis of the present polymerization system, which was based on spreading coefficients, supported the likely occurrence of such nonspherical particles due to the combined effects of interfacial free energies and phase separation between the two polymer phases. A hypothetical pathway was proposed to prepare hydrophilic core-hydrophobic shell composite latex particles, which is based on the concept of opposing driving and resistance forces for the phase migration. It was found that the viscosity of the monomer-swollen polymer phase played important role in the formation of particle morphology.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.16 no.10
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• pp.2273-2279
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• 2012

This research is based on fabricating Cu(In,Ga)$Se_2$ thin-film by co-evaporation method. On $1^{st}$ - stage, $In_2Se_3$ phase appeared when the substrate temperature reached to $400^{\circ}C$, however, there was small effect between the substrate temperature and absorbency spectrum on $2^{nd}$, $3^{rd}$ - stage because the average thickness of the thin-film was $1{\mu}m$ or higher. SEM and XRD was measured on $2^{nd}$ and $3^{rd}$ stage and it showed as the substrate temperature increases, the density of the crystal structure increased with the decreament of the vacancy. Furthermore, the formation of Cu(In0.7Ga0.3)$Se_2$ phase showed at $480^{\circ}C$ and $500^{\circ}C$.

• Journal of Information Technology Applications and Management
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• v.10 no.4
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• pp.65-84
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• 2003

The Purpose of this study is to Identify and analyze the factors that determine the survival or success of internet-based companies in the start-up phase. The reason why we focus on companies which are in the early stage of development is because internet business is still at its early stage, and statistics indicate that many internet businesses have failed during the start-up phase. Using the model of new product outcomes proposed by Cooper(1979), we have formulated a conceptual model for more successful internet business ventures. On the basis of this model, the relationship between the financial and non-financial performance and various variables in the model is analyzed. For collecting empirical data, a web-based survey questionnaire was designed. A total of 97 usable questionnaires were gathered from internet companies nationwide. The results showed that controllable variables such as the commercial entity and information acquired during the new business process are related to the non-financial performance, while environment variables such as the compatibility of the resource base of the firm with the business, including a few controllable variables, are related to the financial performance. Future research needs to explore more precisely how the success factors are different according to the types of internet businesses and/or the stages of growth of the business.

• Applied Microscopy
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• v.16 no.2
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• pp.75-91
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• 1986

Differentiation of the rat testis was studied by light and electron microscope from the fetal stage up to the newborn or adult stage. The purpose of the present study is to investigate the ultrastructural changes of seminiferous tubules and interstitial tissue during the developmental process. The results were as follows: the seminiferous tubule diameter began to increase from birth and was fully developed at 30 to 40 days of age through intratubular cell proliferations. Basement membrane and myoid cells lining the seminiferous tubules were differentiated at 17 days gestation. At the fetal stage, seminiferous tubules were primarily composed of Sertoli cells and the differentiation of Sertoli and germ cells progressed from the newborn stage. Spermatids and immature spermatozoa are appeared at 40 days of age, so from this time, spermatogenesis occurred actively until the adult stage. Sertoli cells aided germ cell differentiation and phagocytosed the parts of the spermatid cytoplasm. Leydig ce]] development follows a biphasic pattern: a fetal phase and then an adult phase from 20 days of age. In conclusion, the rat testis is already developed to some extent by the fetal stage and is functional after 50 days of age. Therefore, these findings indicate that differentiation of Sertoli and Leydig cells precedes the onset of spermatogenesis.

• The Transactions of the Korean Institute of Power Electronics
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• v.19 no.5
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• pp.422-430
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• 2014

This study proposes a small-signal model and control design for a two-stage DC-DC-AC converter to investigate its dynamic characteristics in relation to battery energy storage system. When the circuit analysis of the two-stage DC-DC-AC converter is attempted simultaneously, the mathematical procedure of deriving the dynamic equation is complex and difficult. The main idea of modeling the two-stage DC-DC-AC converter states that this topology is separated into a bidirectional DC-DC converter and a single-phase inverter with an equivalent current source corresponding to that of the inverter or converter. The dynamic equations for the separated converter and inverter are then derived using the state-space averaging technique. The procedures of building the small-signal model of the two-stage DC-DC-AC converter are described in detail. Based on the derived small-signal model, the individual controllers are designed through a frequency-domain analysis. The simulation and experimental results verify the validity of the proposed modeling approach and controller design.