• 제목/요약/키워드: Phase identification

검색결과 698건 처리시간 0.022초

geoA 유전자를 이용한 사상형 남조류(Nostocales, Oscillatoriales)의 Geosmin 생성능 검출 (Detection of Geosmin Production Capability Using geoA Gene in Filamentous Cyanobacteria (Nostocales, Oscillatoriales) Strains)

  • 류희성;신라영;서경애;이정호;김경현
    • 한국물환경학회지
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    • 제34권6호
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    • pp.661-668
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    • 2018
  • Geosmin is volatile metabolites produced by a range of filamentous cyanobacteria which causes taste and odor problems in drinking water. Molecular ecological methods which target biosynthetic genes (geoA) are widely adopted to detect geosmin-producing cyanobacteria. The aim of this study was to investigate the potential production capability of 8 strains isolated from the Nakdong River. Ultimately, a suggestion for a genetical monitoring tool for the identification of geosmin producers in domestic waters was to be made. Geosmin was detected using solid phase microextraction gas chromatography mass spectrometry (SPME GC-MS) in two strains of Dolichospermum plactonicum (DGUC006, DGUC012) that were cultured for 28 day. The highest concentrations during the experiment period was $17,535ngL^{-1}$ and $14,311ngL^{-1}$ respectively. Additionally, geoA genes were amplified using two primers (geo78F/971R and geo78F/982R) from strains shown to produce geosmin, while amplification products were not detected in any of non-producing strains. PCR product (766 bp) was slightly shorter than the expected size for geosmin producers. According to the BLAST analysis, amplified genes were at nucleotide level with Anabaena ucrainica (HQ404996, HQ404997), Dolichospermum planctonicum (KM13400) and Dolichospermum ucrainicum (MF996872) between 99 ~ 100 %. Both strains were thus confirmed as potential geosmin-producing species. We concluded that the molecular method of analysis was a useful tool for monitoring potential cyanobacterial producers of geosmin.

Bridge Inspection and condition assessment using Unmanned Aerial Vehicles (UAVs): Major challenges and solutions from a practical perspective

  • Jung, Hyung-Jo;Lee, Jin-Hwan;Yoon, Sungsik;Kim, In-Ho
    • Smart Structures and Systems
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    • 제24권5호
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    • pp.669-681
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    • 2019
  • Bridge collapses may deliver a huge impact on our society in a very negative way. Out of many reasons why bridges collapse, poor maintenance is becoming a main contributing factor to many recent collapses. Furthermore, the aging of bridges is able to make the situation much worse. In order to prevent this unwanted event, it is indispensable to conduct continuous bridge monitoring and timely maintenance. Visual inspection is the most widely used method, but it is heavily dependent on the experience of the inspectors. It is also time-consuming, labor-intensive, costly, disruptive, and even unsafe for the inspectors. In order to address its limitations, in recent years increasing interests have been paid to the use of unmanned aerial vehicles (UAVs), which is expected to make the inspection process safer, faster and more cost-effective. In addition, it can cover the area where it is too hard to reach by inspectors. However, this strategy is still in a primitive stage because there are many things to be addressed for real implementation. In this paper, a typical procedure of bridge inspection using UAVs consisting of three phases (i.e., pre-inspection, inspection, and post-inspection phases) and the detailed tasks by phase are described. Also, three major challenges, which are related to a UAV's flight, image data acquisition, and damage identification, respectively, are identified from a practical perspective (e.g., localization of a UAV under the bridge, high-quality image capture, etc.) and their possible solutions are discussed by examining recently developed or currently developing techniques such as the graph-based localization algorithm, and the image quality assessment and enhancement strategy. In particular, deep learning based algorithms such as R-CNN and Mask R-CNN for classifying, localizing and quantifying several damage types (e.g., cracks, corrosion, spalling, efflorescence, etc.) in an automatic manner are discussed. This strategy is based on a huge amount of image data obtained from unmanned inspection equipment consisting of the UAV and imaging devices (vision and IR cameras).

비행 데이터 분석 기반의 불안정 접근 경감방안 (Unstable Approach Mitigation Based on Flight Data Analysis)

  • 김현덕
    • 한국항행학회논문지
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    • 제25권1호
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    • pp.52-59
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    • 2021
  • IATA (International Air Transport Assosiation)의 운송용 항공기 사고 집계에 의하면, 사고 건의 61%가 비행의 접근 및 착륙단계에서 발생 되었으며, 이중 항공기의 불안정한 접근으로 인한 사고가 16%인 것으로 파악되었다. 이는 조종사의 불안정한 접근 및 수정 조작 미흡에 따른 복행을 시행하지 않고, 무리하게 접근을 지속하여 사고로 이어진 것으로 식별되었다. 불안정 접근의 원인으로는 공항의 접근 절차, 조종사의 실수, 잘못된 계획, 업무 부하 (workload), ATC (Air Traffic Control) 혼잡 등 다양하게 있을 수 있다. 본 연구에서는 비행 데이터 분석 시스템을 활용하여, 불안정 접근 이벤트가 반복해서 발생하는 국내의 사례 공항과 기종을 선정하여, 주요 발생 이벤트, 공항 접근 절차, 조종사의 조작 뿐만이 아니라 공항의 기상, 지리적 여건 등 다양한 환경적 요인을 포함한 비행 데이터 분석을 통해 불안정 접근 이벤트의 경향성과 발생 원인 및 위험 요소를 식별하고 제거하여 불안정 접근 이벤트의 경감조치 및 항행 안전 증진 방안 마련의 시사점을 도출하고자 한다.

음양변증(陰陽辨證)의 정의와 기능 및 판별방법 연구 (Definition, Role and Method of Yinyang Pattern Differentiation)

  • 지규용;박신형
    • 동의생리병리학회지
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    • 제35권2호
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    • pp.47-55
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    • 2021
  • In order to ensure the fact that eight principle pattern differentiation is used clinically as a basic guideline for Korean medicine practice, the definition, role and method of yin-yang pattern differentiation with its case report were explored at first. Yinyang Pattern Differentiation is a method of discriminating human tendencies or morbidity based on the yin and yang characteristics expressed in living bodies. And yin and yang are the two contrasting characteristics and aspects of the interaction when certain physical conditions that have a lasting effect on the human physiological metabolic function are correlated with the morbidity. Specific methods of yinyang pattern differentiation can be divided into several types of yin and yang indicators. First, time and space factors like day and night, hot and cold seasons, above and below, topographical districts. Second, colors and pulse and their/or relative clearness and muddiness, hardness and softness, moving and resting. Third, diagnose yin and yang patterns through distinguishing the true and false of a fever and cold in an emergency phase such as increase of brain pressure and shock state. Fourth, general characteristics of the propensity and constitution of a subject such as body type, speech, behavior, and physiological metabolism. And for clinical use, these were summarized again as a symptom indicators of physical signs and color, pulse, tongue and questionnaire indicators of propensity, body type, and space-time characteristics. Conclusively, it was confirmed that yinyang pattern differentiation has its own diagnostic significance which is distinct from exterior-interior, cold-heat and deficiency-excess pattern differentiation.

A multi-layer approach to DN 50 electric valve fault diagnosis using shallow-deep intelligent models

  • Liu, Yong-kuo;Zhou, Wen;Ayodeji, Abiodun;Zhou, Xin-qiu;Peng, Min-jun;Chao, Nan
    • Nuclear Engineering and Technology
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    • 제53권1호
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    • pp.148-163
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    • 2021
  • Timely fault identification is important for safe and reliable operation of the electric valve system. Many research works have utilized different data-driven approach for fault diagnosis in complex systems. However, they do not consider specific characteristics of critical control components such as electric valves. This work presents an integrated shallow-deep fault diagnostic model, developed based on signals extracted from DN50 electric valve. First, the local optimal issue of particle swarm optimization algorithm is solved by optimizing the weight search capability, the particle speed, and position update strategy. Then, to develop a shallow diagnostic model, the modified particle swarm algorithm is combined with support vector machine to form a hybrid improved particle swarm-support vector machine (IPs-SVM). To decouple the influence of the background noise, the wavelet packet transform method is used to reconstruct the vibration signal. Thereafter, the IPs-SVM is used to classify phase imbalance and damaged valve faults, and the performance was evaluated against other models developed using the conventional SVM and particle swarm optimized SVM. Secondly, three different deep belief network (DBN) models are developed, using different acoustic signal structures: raw signal, wavelet transformed signal and time-series (sequential) signal. The models are developed to estimate internal leakage sizes in the electric valve. The predictive performance of the DBN and the evaluation results of the proposed IPs-SVM are also presented in this paper.

산지별 밤꿀에 함유된 Kynurenic Acid의 정량 분석과 분석법 검증 (Quantitative Analysis of Kynurenic Acid in Chestnut Honey from Different Regions and Method Validation)

  • 김주리;김도윤;이상현
    • 생약학회지
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    • 제53권2호
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    • pp.111-118
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    • 2022
  • Chestnut honey is a sweet dark-colored honey with a distinct bitter aftertaste. It contains numerous phenolic compounds and alkaloids and is noted for its antioxidant and anti-inflammatory activities. However, it has been established that there are differences in the composition and activity of chestnut honey constituents depending on the region of origin, the sources of which warrant further research. In this study, we analyzed the kynurenic acid (KA) contents in chestnut honey produced in nine different regions in Korea, using high-performance liquid chromatography in conjunction with ultraviolet detection, and validated the analytical method developed. Use of a reverse-phase column and detection at a wavelength of 240 nm were found to be optimal for the detection of KA. Similar evaluation of an optimal method for extracting KA from chestnut honey revealed that extraction using 10% EtOH at 20 times the sample volume over a 6 h period was the most suitable for obtaining a high content of KA. Among the nine regional chestnut honeys assessed, KA content was found to be highest in the "Gongju" sample (1.14 mg/g), followed by that in the "Cheongdo" and "Damyang" samples. Validation of the KA analytical method revealed a good analyte linearity, with a correlation coefficient (r2) of 0.9995, an accuracy of between 92.37% and 107.35%, and good precision (RSD ≤ 1.05%). Our findings in this study, based on a validated quantitative analytical method for KA, could make an important contribution to establishing a data profiling procedure for characterizing chestnut honeys produced in different regions, and may also provide basic data for the identification of functional honey.

Fabrication and Evaluation of a Total Organic Carbon Analyzer Using Photocatalysis

  • Do Yeon Lee;Jeong Hee Shin;Jong-Hoo Paik
    • 센서학회지
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    • 제32권3호
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    • pp.140-146
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    • 2023
  • Water quality is crucial for human health and the environment. Accurate measurement of the quantity of organic carbon in water is essential for water quality evaluation, identification of water pollution sources, and appropriate implementation of water treatment measures. Total organic carbon (TOC) analysis is an important tool for this purpose. Although other methods, such as chemical oxygen demand (COD) and biochemical oxygen demand (BOD) are also used to measure organic carbon in water, they have limitations that make TOC analysis a more favorable option in certain situations. For example, COD requires the use of toxic chemicals, and BOD is time-consuming and can produce inconsistent and unreliable results. In contrast, TOC analysis is rapid and reliable, providing accurate measurements of organic carbon content in water. However, common methods for TOC analysis can be complex and energy-intensive because of the use of high-temperature heaters for liquid-to-gas phase transitions and the use of acid, which present safety risks. This study focuses on a TOC analysis method using TiO2 photocatalysis, which has several advantages over conventional TOC analysis methods, including its low cost and easy maintenance. For TiO2, rutile and anatase powders are mixed with an inorganic binder and spray-coated onto a glass fiber substrate. The TiO2 powder and inorganic binder solutions are adjusted to optimize the photocatalytic reaction performance. The TiO2 photocatalysis method is a simple and low-power approach to TOC analysis, making it a promising alternative to commonly used TOC analysis methods. This study aims to contribute to the development of more efficient and cost-effective approaches for water quality analysis and management by exploring the effectiveness and reliability of the developed equipment.

CA2 혼입 페이스트의 부식저항성에 관한 연구 (A Study on Corrosion Resistance of CA2-Mixed Paste)

  • 김재돈;장일영
    • 한국산업융합학회 논문집
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    • 제25권2_2호
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    • pp.289-297
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    • 2022
  • Deterioration in durability of structures due to the steel corrosion is difficult to determine whether or not corrosion is initiated and how much propagated, and moreover, repair and maintenance are not easy to deal with. Therefore, preventive treatments can be the best option to avoid the deterioration. Various methods for preventing corrosion of steel, such as electrochemical treatments, anti-corrosion agents and steel surface coatings, are being developed, but economic and environmental aspects make it difficult to apply them to in-situ field. Thus, the purpose of this study was to improve corrosion resistance by using CA-based clinker that are relatively simple and expected to be economically profitable Existing CA-based clinkers had problems such as flash setting and low strength development during the initial hydration process, but in order to solve this problem, CA clinker with low initial reactivity were used as binder in this study. The cement paste used in the experiments was replaced with CA2 clinker for 0%, 10%, 20%, and 30% in OPC. And the mixture used in the chloride binding test for the extraction of water-soluble chloride was intermixed with Cl- 0.5%, 1%, 2%, and 3% by weight of binder content. To evaluate characteristic of hydration heat evolution, calorimetry analysis was performed and simultaneously chloride binding capacity and acid neutralization capacity were carried out. The identification of hydration products with curing ages was verified by X-ray diffraction analysis. The free chloride extraction test showed that the chlorine ion holding ability improved in order OC 10 > OC 30 > OC 20 > OC 0 and the pH drop resistance test showed that the resistance capability in pH 12 was OC 0 > OA 10 > OA 20 > OA 30. The XRD analyses showed that AFm phase, which can affect the ability to hold chlorine ions, tended to increase when CA2 was mixed, and that in pH12 the content of calcium hydroxide (Ca(OH)2), which indicates pH-low resistance, decreased as CA2 was mixed

Ultrasonic guided waves-based fatigue crack detection in a steel I-beam: an experimental study

  • Jiaqi Tu;Xian Xu;Chung Bang Yun;Yuanfeng Duan
    • Smart Structures and Systems
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    • 제31권1호
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    • pp.13-27
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    • 2023
  • Fatigue crack is a fatal problem for steel structures. Early detection and maintenance can help extend the service life and prevent hazards. This paper presents the ultrasonic guided waves-based (UGWs-based) fatigue crack detection of a steel I-beam. The semi-analytical finite element model has been built to obtain the wave propagation characteristics. Damage indices in both time and frequency domains were analyzed by considering the characteristic variations of UGWs including the amplitude, phase angle, and wave packet energy. The pulse-echo and pitch-catch methods were combined in the detection scheme. Lab-scale experiments were conducted on welded steel I-beams to verify the proposed method. Results show that the damage indices based on the characteristic variations in the time domain can identify and localize the fatigue crack before it enters the rapid growth stage. The damage severity can be reasonably evaluated by analyzing the time-domain damage indices. Two nonlinear damage indices in the frequency domain give earlier warnings of the fatigue crack than the time-domain damage indices do. The identification results based on the above two nonlinear indices are found to be less consistent under various excitation frequencies. More robust nonlinear techniques needed to be searched and tested for early crack detection in steel I-beams in further study.

부산권역 급성 허혈성 뇌졸중 환자 이송 및 치료단계에서 독거가 미치는 영향 (The Impact of Living Alone on the Transfer and Treatment Stages of Acute Ischemic Stroke in the Busan Metropolitan Area)

  • 정혜인;김선정;김병권;차재관
    • 보건행정학회지
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    • 제33권4호
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    • pp.440-449
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    • 2023
  • Background: This study aimed to analyze the prehospital process and reperfusion therapy process of acute ischemic stroke in Busan metropolitan area and examine the impact of living arrangement on the early management and functional outcomes of acute ischemic stroke (AIS). Methods: The patients who diagnosed with AIS and received reperfusion therapy at the Busan Regional Cardiovascular Center between September 2020 and May 2023 were selected. We investigated the patients' hospital arrival time (onset to door time) and utilization of 119 emergency ambulance services. Additionally, various time matrices related to reperfusion therapy after hospital were examined, along with the functional outcome at the 90-day after treatment. Results: Among the 753 AIS patients who underwent reperfusion therapy, 166 individuals (22.1%) were living alone. AIS patients living alone experienced significant delays in symptom detection (p<0.05) and hospital arrival compared to AIS patients with cohabitants (370.1 minutes vs. 210.2 minutes, p<0.001). There were no significant differences between the two groups in terms of 119 ambulance utilization and time metrics related with the reperfusion therapy. Independent predictors of prognosis in AIS patients were found to be age above 70, National Institutes of Health Stroke Scale score at admission, tissue plasminogen activator, living alone (odds ratio [OR], 1.785; 95% confidence interval [CI], 1.155-2.760) and interhospital transfer (OR, 1.898; 95% CI, 1.152-3.127). Delay in identification of AIS was shown significant correlation (OR, 2.440; 95% CI, 1.070-5.561) at living alone patients. Conclusion: This study revealed that AIS patients living alone in the Busan metropolitan region, requiring endovascular treatment, face challenges in the pre-hospital phase, which significantly impact their prognosis.