• Title/Summary/Keyword: Phase identification

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Concentration Characteristics of Indoor and Outdoor Airborne Total Fiber Particles and Identification of Asbestos in Gyeongnam Provinces (경남지역의 실내외 공기 중 총섬유 입자의 농도특성 및 석면 입자의 확인)

  • Park, Hee-Eun;Park, Jeong-Ho;Kim, Hyoung-Kab
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.22 no.2
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    • pp.119-127
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    • 2012
  • Objectives: The aim of this study is to identify concentration characteristics of indoor and outdoor airborne total fiber particles and asbestos in Gyeongnam Provinces. Methods: This study investigated concentration characteristics of indoor fiber particles from 748 schools and 38 public facilities as well as outdoor particles from 11 sites through PCM (phase contrast microscope). SEM/EDX (scanning electron microscope/energy dispersive using X-ray analysis) was used to obtain physicochemical information of asbestos fiber particles. The study identified asbestos rate in the 15 samples from indoor and outdoor airborne total fiber particles. Results: 1. The average indoor airborne concentrations of total fiber particles were $0.0011{\pm}0007$ f/cc in schools and $0.0015{\pm}0007$ f/cc in public facilities by PCM. Over 90% of the fiber particles were identified as single fibers. 2. The average outdoor airborne concentrations of total fiber particles were $0.0007{\pm}0002$ f/cc, and they were lower than those of indoor airborne concentrations. 3. The results showed that the form of asbestiform was diverse as skein of thread like form and long needle, which was relatively narrower than that of glass fiber and rock wool. 4. The results of SEM/EDX analysis of 15 areas where total fiber particle was relatively high showed that the form was rather similar to that of asbestos, but chemical composition was proven to be non-asbestos. Conclusions: The concentration of indoor and outdoor airborne total fiber particles of Gyeongnam Provinces satisfied the IAQ (Indoor air quality) level of 0.01 f/cc and asbestos was not found in most of the samples by SEM/EDX.

Identification and Characterization of Two Novel Variants of the DUF1208 Protein FAM92A1

  • Ruan, Xu Zhi;Yan, Fei;Zhao, Xin Yu;Wang, Chung Ting;Song, Ming;Yang, Han Suo;Deng, Hong Xin;Wei, Yu Quan
    • Molecules and Cells
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    • v.23 no.3
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    • pp.391-397
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    • 2007
  • FAM92A1 (named FAM92A1-271) belongs to the family of proteins with conserved DUF1208 domains. Its function remains elusive. We identified two novel transcript variants (FAM92A1-251, FAM92A1-289) of FAM92A1. The presence of these transcripts in cancerous and normal cells, as well as their influence on cell prolifera-tion and apoptosis, were investigated. The subcellular location of FAM92A1 was determined by fluorescence microscopy. We found that FAM92A1-271 and FAM92A1-289 were highly expressed in both normal and cancerous cells, but FAM92A1-251 was only expressed at a mo-derate level in both types of cell. Overexpression of FAM92A1-271, FAM92A1-251 and FAM92A1-289 inhibited cell proliferation, caused S-phase arrest and induced apoptosis. Subcellular localization showed that FAM92A1 localizes to the nucleus. Our results show that FAM92A1 has different splicing variants, and that it may take part in regulating cell proliferation and apoptosis.

Identification of Guided-Wave Modes in Pipings of Power Plants by using Air-coupled Transducer (Air-coupled 트런스듀서를 이용한 발전설비 배관에서의 유도초음파 모드 규명)

  • Park, Ik-Keun;Kim, Hyun-Mook;Kim, Yong-Kwon;Song, Won-Joon;Cho, Yong-Sang;Jhang, Kyung-Young;Cho, Youn-Ho
    • Journal of the Korean Society for Nondestructive Testing
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    • v.24 no.4
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    • pp.341-347
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    • 2004
  • In order to inspect the piping effectively, one of the important components in the facility of power plants, the ultrasonic guided wave was generated by a tomb transducer and was received in a non-contact fashion by using an air-coupled transducer. The guided wave modes that ran be generated by the comb transducer in piping are predicted from the theoretical dispersion curves and the element spacing of a comb transducer. Moreover, to receive the specific modes, the receiving angle of the air-coupled transducer is calculated from Snell's law between the phase velocities of guided waves and the sound velocity of air. The guided wave modes obtained in experiments are identified from the result of time-frequency analysis such as wavelet transform and two-dimensional fast Fourier transform.

Analysis of $\triangle^9$-Tetrahydrocannabinol and 11-nor-9-carboxytetrahydrocannabinol in Hair by Gas Chromatography/Mass Spectrometry (가스크로마토그라피/질량분석기에 의한 모발중 대마성분 분석)

  • 양원경;한은영;박용훈;임미애;정희선
    • YAKHAK HOEJI
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    • v.48 no.3
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    • pp.207-212
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    • 2004
  • An analytic method was developed for the quantitation of $\Delta$$^{9}-$ tetrahydrocannabinol (THC) and 11-nor-9-carboxy THC (THC-COOH) in human hair. After hair samples were pulverized using Freezer Mill, deuterated internal standards were added and digested in 1 N NaOH at $100^{\circ}C$ water bath for 30 min. Digest solutions were extracted by 5 ml hexane:ethyl acetate (90:10) after acidification with acetic acid. The organic phase was evaporated under N 2 and derivatized by BSTFA (with 1% TMCS) at $85^{\circ}C$ for 45 min. The derivatized solution was separated on HP-5MS column ($30m{\times}0.25mm{\times}0.25mm$) and detected using EI-GC-MS with selective ion monitoring mode. The assay of calibration was ranged from 5 to 100 ng/50 mg hair ($r^2$>0.99) for THC and THC-COOH. Within and between-run precision were calculated at 6, 30, 60 ng/50 mg hair with coefficients of variation less than 11%. Within and between run accuracies at the same concentrations were$\pm$14% and $\pm$30% of target for both analytes, respectively. Absolute and relative recovery at 10 and 100 ng were 60∼91%. The method was used to detect and quantify THC and THC-COOH in cannabis abuser's hairs (N = 16) and SRM (N=5, THC 1 ng/mg, NIST). We detected THC and THC-COOH in only one hair sample. In SRM, % accuracy was 93% (range 86∼103%) and precision (% CV) was 8.14. We began to set up a quantitative analysis of THC and THC-COOH using EI-GC-MS. Continuously, we need to modify and develop this method in order to apply for identification in cannanbis users' hair.

Removal of Super-Refraction Echoes using X-band Dual-Polarization Radar Parameters (X-밴드 이중편파 레이더 변수를 이용한 과대굴절에코 제거)

  • Seo, Eun-Kyoung;Kim, Dong Young
    • Journal of the Korean earth science society
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    • v.40 no.1
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    • pp.9-23
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    • 2019
  • Super-refraction of radar beams tends to occur primarily under a particular vertical structure of temperature and water vapor pressure profiles. A quality control process for the removal of anomalous propagation (AP) ehcoes are required because APs are easily misidentified as precipitation echoes. For this purpose, we collected X-band polarimetric radar parameters (differential reflectivity, cross-correlation coefficient, and differential phase) only including non-precipitation echoes (super-refraction and clear-sky ground echoes) and precipitation echoes, and compared the echo types regarding the relationships among radar reflectivities, polarimetric parameters, and the membership functions. We developed a removal algorithm for the non-precipitation echoes using the texture approach for the polarimetric parameters. The presented algorithm is qualitatively validated using the S-band Jindo radar in Jeollanam-do. Our algorithm shows the successful identification and removal of AP echoes.

Modeling Method of Receiving Radar Signals from Warhead and Decoy with Micro-Motion (미세운동을 가지는 탄두 및 기만체의 새로운 레이다 수신신호 모델링 방법)

  • Choi, In-Oh;Park, Sang-Hong;Kang, Ki-Bong;Kim, Kyung-Tae
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.30 no.3
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    • pp.243-251
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    • 2019
  • Recently, several studies were conducted on the micro-Doppler(MD) phenomenon to identify a warhead from decoys. Both, the warhead and decoy, can be modeled as various shapes and maneuver with their own micro-motion. Their MD phenomenon can be demonstrated by amplitude modulation and phase modulation. Most studies have utilized approximate solutions to express the amplitude modulation regardless of various warhead and decoy shapes. However, the exact solution of the amplitude modulation is required for more effective warhead identification. In this study, we proposed a new modeling method of receiving radar signals from warheads and decoys based on physical optics. The proposed solution was evaluated using an electromagnetic prediction technique and computer-aided design models.

Gastric Follicular Lymphomas Presenting as Subepithelial Tumors: Two Cases (위 상피하 종양으로 발견된 소포림프종 2예)

  • Kim, Hyeong Jin;Choi, Cheol Woong;Park, Su Bum;Kim, Su Jin
    • The Korean journal of helicobacter and upper gastrointestinal research
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    • v.18 no.4
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    • pp.258-263
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    • 2018
  • Follicular lymphoma is the most common form of low-grade B cell lymphoma. Follicular lymphoma occurs predominantly at lymph node sites and rarely in the gastrointestinal tract. Rare gastrointestinal follicular lymphoma is most commonly found in the small intestine, especially in the duodenum, and appears as multiple granules. However, gastric follicular lymphoma mostly appears as a subepithelial tumor. We observed two primary gastric follicular lymphomas that resembled subepithelial tumors located in the body of the stomach. Endoscopic ultrasound revealed hypoechoic lesions located in the submucosa layer. Since endoscopic forceps biopsies were inconclusive, we performed endoscopic submucosal dissection, which resulted in a final pathologic diagnosis of follicular lymphoma. Because of the indolent nature of gastrointestinal follicular lymphoma, the "watch and wait" strategy can be applied in the early phase. The identification of endoscopic characteristics of gastric follicular lymphoma can be helpful for differential diagnosis and decision of treatment strategy. Therefore, we report two cases of primary gastrointestinal follicular lymphoma diagnosed following endoscopic submucosal dissection.

Identification of phospholipase Cβ downstream effect on transient receptor potential canonical 1/4, transient receptor potential canonical 1/5 channels

  • Ko, Juyeon;Myeong, Jongyun;Kwak, Misun;Jeon, Ju-Hong;So, Insuk
    • The Korean Journal of Physiology and Pharmacology
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    • v.23 no.5
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    • pp.357-366
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    • 2019
  • $G{\alpha}_q$-coupled receptor stimulation was implied in the activation process of transient receptor potential canonical (TRPC)1/4 and TRPC1/5 heterotetrameric channels. The inactivation occurs due to phosphatidylinositol 4,5-biphosphate ($PI(4,5)P_2$) depletion. When $PI(4,5)P_2$ depletion was induced by muscarinic stimulation or inositol polyphosphate 5-phosphatase (Inp54p), however, the inactivation by muscarinic stimulation was greater compared to that by Inp54p. The aim of this study was to investigate the complete inactivation mechanism of the heteromeric channels upon $G{\alpha}_q$-phospholipase $C{\beta}$ ($G{\alpha}_q-PLC{\beta}$) activation. We evaluated the activity of heteromeric channels with electrophysiological recording in HEK293 cells expressing TRPC channels. TRPC1/4 and TRPC1/5 heteromers undergo further inhibition in $PLC{\beta}$ activation and calcium/protein kinase C (PKC) signaling. Nevertheless, the key factors differ. For TRPC1/4, the inactivation process was facilitated by $Ca^{2+}$ release from the endoplasmic reticulum, and for TRPC1/5, activation of PKC was concerned mostly. We conclude that the subsequent increase in cytoplasmic $Ca^{2+}$ due to $Ca^{2+}$ release from the endoplasmic reticulum and activation of PKC resulted in a second phase of channel inhibition following $PI(4,5)P_2$ depletion.

Detection of Geosmin Production Capability Using geoA Gene in Filamentous Cyanobacteria (Nostocales, Oscillatoriales) Strains (geoA 유전자를 이용한 사상형 남조류(Nostocales, Oscillatoriales)의 Geosmin 생성능 검출)

  • Ryu, Hui-Seong;Shin, Ra-Young;Seo, Kyung-Ae;Lee, Jung-Ho;Kim, Kyunghyun
    • Journal of Korean Society on Water Environment
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    • v.34 no.6
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    • pp.661-668
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    • 2018
  • Geosmin is volatile metabolites produced by a range of filamentous cyanobacteria which causes taste and odor problems in drinking water. Molecular ecological methods which target biosynthetic genes (geoA) are widely adopted to detect geosmin-producing cyanobacteria. The aim of this study was to investigate the potential production capability of 8 strains isolated from the Nakdong River. Ultimately, a suggestion for a genetical monitoring tool for the identification of geosmin producers in domestic waters was to be made. Geosmin was detected using solid phase microextraction gas chromatography mass spectrometry (SPME GC-MS) in two strains of Dolichospermum plactonicum (DGUC006, DGUC012) that were cultured for 28 day. The highest concentrations during the experiment period was $17,535ngL^{-1}$ and $14,311ngL^{-1}$ respectively. Additionally, geoA genes were amplified using two primers (geo78F/971R and geo78F/982R) from strains shown to produce geosmin, while amplification products were not detected in any of non-producing strains. PCR product (766 bp) was slightly shorter than the expected size for geosmin producers. According to the BLAST analysis, amplified genes were at nucleotide level with Anabaena ucrainica (HQ404996, HQ404997), Dolichospermum planctonicum (KM13400) and Dolichospermum ucrainicum (MF996872) between 99 ~ 100 %. Both strains were thus confirmed as potential geosmin-producing species. We concluded that the molecular method of analysis was a useful tool for monitoring potential cyanobacterial producers of geosmin.

Bridge Inspection and condition assessment using Unmanned Aerial Vehicles (UAVs): Major challenges and solutions from a practical perspective

  • Jung, Hyung-Jo;Lee, Jin-Hwan;Yoon, Sungsik;Kim, In-Ho
    • Smart Structures and Systems
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    • v.24 no.5
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    • pp.669-681
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    • 2019
  • Bridge collapses may deliver a huge impact on our society in a very negative way. Out of many reasons why bridges collapse, poor maintenance is becoming a main contributing factor to many recent collapses. Furthermore, the aging of bridges is able to make the situation much worse. In order to prevent this unwanted event, it is indispensable to conduct continuous bridge monitoring and timely maintenance. Visual inspection is the most widely used method, but it is heavily dependent on the experience of the inspectors. It is also time-consuming, labor-intensive, costly, disruptive, and even unsafe for the inspectors. In order to address its limitations, in recent years increasing interests have been paid to the use of unmanned aerial vehicles (UAVs), which is expected to make the inspection process safer, faster and more cost-effective. In addition, it can cover the area where it is too hard to reach by inspectors. However, this strategy is still in a primitive stage because there are many things to be addressed for real implementation. In this paper, a typical procedure of bridge inspection using UAVs consisting of three phases (i.e., pre-inspection, inspection, and post-inspection phases) and the detailed tasks by phase are described. Also, three major challenges, which are related to a UAV's flight, image data acquisition, and damage identification, respectively, are identified from a practical perspective (e.g., localization of a UAV under the bridge, high-quality image capture, etc.) and their possible solutions are discussed by examining recently developed or currently developing techniques such as the graph-based localization algorithm, and the image quality assessment and enhancement strategy. In particular, deep learning based algorithms such as R-CNN and Mask R-CNN for classifying, localizing and quantifying several damage types (e.g., cracks, corrosion, spalling, efflorescence, etc.) in an automatic manner are discussed. This strategy is based on a huge amount of image data obtained from unmanned inspection equipment consisting of the UAV and imaging devices (vision and IR cameras).