• Title/Summary/Keyword: Phase identification

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Isolation and Characterization of an Alkaline Protease Produced by Bacillus subtilis JK-1 (알칼리성 Pretease를 생산하는 Bacillus subtilis JK-1의 분리 동정 및 효소 특성)

  • Kim, Ji-Yeon
    • Korean Journal of Microbiology
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    • v.43 no.4
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    • pp.331-336
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    • 2007
  • A bacterium producing the alkaline pretense was isolated from Chungkookjoug, and was identified as Bacillus subtilis JK-1 based on morphological, physiological and biochemical characteristics, as well as phylogenetic analysis using 16S rRNA gene sequence. The optimum pH and temperature of the pretense activity were pH 9.0 and $55^{\circ}C$, respectively. This enzyme was stable at the temperatures $40{\sim}80^{\circ}C$. The maximum alkaline pretense production was obtained when 1.0% (w/v) xylose, 1.0% (w/v) yeast extract and 0.3% (w/v) $CuSO_4$ were used as carbon source, nitrogen source and mineral source. Under the optimal condition, growth of the isolate was reached at stationary phase after 12 hr followed by incubation, the alkaline pretense production reached a maximum level with $16{\sim}20$ hr cultivation.

A Study on the Identification of the Standardized Modulation Type for the Telephone Channel (전화채널용 표준 변조방식의 식별에 관한 연구)

  • 김병진;조동호;이황수;고봉수
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.16 no.3
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    • pp.207-218
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    • 1991
  • In this paper, the characteristics of MODEM with the low and medium transmission rate which are recommended by the international organization such as CCITT have been studied and the features of the telephone channel have been investigated. Then three kinds of classification algorithm for the modulated signals have been suggested and their performance have been researched through the computer simulation. In case that the channel is ideal, the modulated type such as FSK, PSK and QAM could be detected correctly. Moreover, when the modulated signals are received through the telpehone channel or noisy channel, it could be seen that the performance of the correlation algorithm is superior to that of other two algorithms because of the correlation being robust to the characteristics of telephone chanel and Gasussian noise, although the performances of the two classification algorithm using the peak and phase difference, signal constellation and so on decrease rapidly as the amplitude distortion, delay distortion and Gaussian noise increase.

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Schedule Risk Management of Reinforced Concrete Construction at the Preconstruction Phase (착공전 단계에서의 철근콘크리트 공사 공정리스크 관리 방안)

  • Ryu, Han-Guk;Kim, Sun-Kuk;Lee, Hyun-Soo
    • Korean Journal of Construction Engineering and Management
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    • v.6 no.5 s.27
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    • pp.119-127
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    • 2005
  • According to the recent systemic changes such as construction market reduction, five days per week system, introduction of construction duration reduction bidding system and post-construction sale system, the bidding competition have been enhanced. However, there are many construction schedule risks which could delay construction completion. Without the proper identification of construction schedule risks and the structured construction schedule risk management, the competitive advantages of construction project could not be made. Previous researches to solve the problem have been focused on the construction schedule categorization, construction schedule management, and the system development to manage the construction schedule risk. Therefore, this study selected the reinforeced concrete construction as the studied work due to the major impact characteristics on the total construction duration and the quite accident rate, and then proposed the real construction schedule risks of reinforced concrete construction and the construction risk management method based on the previous studies, many times of expert interviews and brainstorming.

Multimode Boundary-Layer Transition on an Airfoil Influenced by Periodically Passing Wake under the Free-stream Turbulence (자유유동 난류 하의 주기적 통과 후류의 영향을 받는 익형 위 경계층 천이)

  • Park Tae-Choon;Jeon Woo-Pyung;Kang Shin-Hyoung
    • Proceedings of the KSME Conference
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    • 2002.08a
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    • pp.687-690
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    • 2002
  • Multimode boundary-layer transition on a NACA0012 airfoil is experimentally investigated under periodically passing wakes and the moderate level of free-stream turbulence. The periodic wakes are generated by rotating circular cylinders clockwise or counterclockwise around the airfoil. The free-stream turbulence is produced by a grid upstream of the rotating cylinder, and its intensity(Tu) at the leading edge of the airfoil is $0.5\;or\;3.5\;{\%}$. The Reynolds number ($Re_c$) based on chord length (C) of the alrfoil is $2.0{\times}10^5$, and Strouhal number ($St_c$) of the passing wake is about 0.7. Time- and phase-averaged streamwise mean velocities and turbulence fluctuations are measured with a single hot-wire probe, and especially, the corresponding wall skin friction is evaluated using a computational Preston tube method. The wake-passing orientation changes pressure distribution on the airfoil in a different manner irrespective of the free-stream turbulence. Regardless of free-stream turbulence level, turbulent patches for the receding wakes propagate more rapidly than those for the approaching wake because adverse pressure gradient becomes larger. The patch under the high free-stream turbulence ($Tu=3.5{\%}$) grows more greatly in laminar-like regions compared with that under the low background turbulence ($Tu=0.5{\%}$) in laminar regions. The former, however, does not greatly change the original turbulence level in the very near-wall region while the latter does it. At further downstream, the former interacts vigorously with high environmental turbulence inside the pre-existing transitional boundary layer and gradually lose his identification, whereas the latter keep growing in the laminar boundary layer. The calmed region is more clearly observed under the lower free-stream turbulence level and for the receding wakes. The calmed region delays the breakdown further downstream and stabilizes more the boundary layer.

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Identification and Characterization of a Novel Antioxidant Peptide from Bovine Skim Milk Fermented by Lactococcus lactis SL6

  • Kim, Sang Hoon;Lee, Ji Yoon;Balolong, Marilen P.;Kim, Jin-Eung;Paik, Hyun-Dong;Kang, Dae-Kyung
    • Food Science of Animal Resources
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    • v.37 no.3
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    • pp.402-409
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    • 2017
  • A novel peptide having free radical scavenging activity was separated, using an on-line high-performance liquid chromatography (HPLC) - ABTS screening method, from bovine skim milk fermented by Lactococcus lactis SL6 (KCTC 11865BP). It was further purified using reverse phase-HPLC (RP-HPLC) and sequenced by RP-HPLC-tandem mass spectrometry. The amino acid sequence of the identified peptide was determined to be Phe-Ser-Asp-Ile-Pro-Asn-Pro-Ile-Gly-Ser-Glu-Asn-Ser-Glu-Lys-Thr-Thr-Met-Pro-Leu-Trp (2,362 Da), which is corresponding to the C-terminal fragment of bovine ${\alpha}_{s1}$-casein (f179-199). The hydroxyl radicals scavenging activity ($IC_{50}$ $28.25{\pm}0.96{\mu}M$) of the peptide chemically synthesized based on the MS/MS data showed a slightly lower than that of the natural antioxidant Trolox ($IC_{50}$ $15.37{\pm}0.52{\mu}M$). Furthermore, derivatives of the antioxidant peptide were synthesized. The antioxidative activity of the derivatives whose all three proline residues replaced by alanine significantly decreased, whereas replacement of two proline residues in N-terminal region did not affect its antioxidative activity, indicating that $3^{rd}$ proline in C-terminal region is critical for the antioxidative activity of the peptide identified in this study. In addition, N-terminal region of the antioxidant peptide did not show its activity, whereas C-terminal region maintained antioxidative activity, suggesting that C-terminal region of the peptide is important for antioxidative activity.

Deinterleaving of Multiple Radar Pulse Sequences Using Genetic Algorithm (유전자 알고리즘을 이용한 다중 레이더 펄스열 분리)

  • 이상열;윤기천
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.40 no.6
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    • pp.98-105
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    • 2003
  • We propose a new technique of deinterleaving multiple radar pulse sequences by means of genetic algorithm for threat identification in electronic warfare(EW) system. The conventional approaches based on histogram or continuous wavelet transform are so deterministic that they are subject to failing in detection of individual signal characteristics under real EW signal environment that suffers frequent signal missing, noise, and counter-EW signal. The proposed algorithm utilizes the probabilistic optimization procedure of genetic algorithm. This method, a time-of-arrival(TOA) only strategy, constructs an initial chromosome set using the difference of TOA. To evaluate the fitness of each gene, the defined pulse phase is considered. Since it is rare to meet with a single radar at a moment in EW field of combat, multiple solutions are to be derived in the final stage. Therefore it is designed to terminate genetic process at the prematured generation followed by a chromosome grouping. Experimental results for simulated and real radar signals show the improved performance in estimating both the number of radar and the pulse repetition interval.

Two-Phase Localization Algorithm in Wireless Sensor Networks (무선 센서 네트워크에서의 2단계 위치 추정 알고리즘)

  • Song Ha-Ju;Kim Sook-Yeon;Kwon Oh-Heum
    • Journal of Korea Multimedia Society
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    • v.9 no.2
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    • pp.172-188
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    • 2006
  • Sensor localization is one of the fundamental problems in wireless sensor networks. Previous localization algorithms can be classified into two categories, the GGB (Global Geometry-Based) approaches and the LGB (Local Geometry-Based). In the GGB approaches, there are a fixed set of reference nodes of which the coordinates are pre-determined. Other nodes determine their positions based on the distances from the fixed reference nodes. In the LGB approaches, meanwhile, the reference node set is not fixed, but grows up dynamically. Most GGB algorithms assume that the nodes are deployed in a convex shape area. They fail if either nodes are in a concave shape area or there are obstacles that block the communications between nodes. Meanwhile, the LGB approach is vulnerable to the errors in the distance estimations. In this paper, we propose new localization algorithms to cope with those two limits. The key technique employed in our algorithms is to determine, in a fully distributed fashion, if a node is in the line-of-sight from another. Based on the technique, we present two localization algorithms, one for anchor-based, another for anchor-free localization, and compare them with the previous algorithms.

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An Efficient RFID Distance-Bounding Protocol to Prevent Relay Attacks (중계 공격을 예방하는 효율적인 RFID Distance-Bounding 프로토콜)

  • Boo, Chang-Hee;Jun, Moon-Seog
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.35 no.4B
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    • pp.610-619
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    • 2010
  • RFID (radio frequency identification) systems, recently being used in a wide range of areas, are vulnerable to relay attack from malicious attackers. For that reason, Brands, et al. proposed a certification protocol between a certifier and a verifier based on the concept of distance-bounding, and in addition Hancke et al. introduced the concept of RFID. However, the delivery of tag IDs, one of the main RFID features, is not still available, and there are two important demerits: anonymity in the delivery of tag IDs suggested by Kim et al. and inefficiency in finding a tag ID with regard to how to check errors which may occur in the process of data exchange between readers and tags. Therefore, this study proposes a protocol that meets the requirements of tag anonymity and location untraceability, has resistance to errors which may take place in the phase of tag data exchange, and is very efficient in finding tag IDs.

Characterization of Fe-ACF/TiO2 composite and photocatalytic activity for MB Solution under visible light (Fe-ACF/TiO2 복합체의 특성화와 가시광선조건에서 MB 용액의 광촉매활성)

  • Zhang, Kan;Meng, Ze-Da;Oh, Won-Chun
    • Analytical Science and Technology
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    • v.23 no.3
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    • pp.225-232
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    • 2010
  • In present study, a conventional sol-gel method was used to prepare Fe-ACF/$TiO_2$ composites, a kind of composite photocatalysts, whose capability was evaluated by degrading methylene blue (MB) solution. The particle size, surface structure, crystal phase and elemental identification of the composites prepared were characterized by BET, SEM, XRD and EDX, respectively. The spectra of MB concentration degraded under visible light were obtained by UV/Vis spectrophotometer. These obtained spectra demonstrated the photocaltalytic activity from removal concentrations of MB. It was considered that these photonic activities are induced by a strong synergetic reaction among ACF, $TiO_2$ and Fe in the composite photocatalysts under visible light.

The role of surgical clips in the evaluation of interfractional uncertainty for treatment of hepatobiliary and pancreatic cancer with postoperative radiotherapy

  • Bae, Jin Suk;Kim, Dong Hyun;Kim, Won Taek;Kim, Yong Ho;Park, Dahl;Ki, Yong Kan
    • Radiation Oncology Journal
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    • v.35 no.1
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    • pp.65-70
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    • 2017
  • Purpose: To evaluate the utility of implanted surgical clips for detecting interfractional errors in the treatment of hepatobiliary and pancreatic cancer with postoperative radiotherapy (PORT). Methods and Materials: Twenty patients had been treated with PORT for locally advanced hepatobiliary or pancreatic cancer, from November 2014 to April 2016. Patients underwent computed tomography simulation and were treated in expiratory breathing phase. During treatment, orthogonal kilovoltage (kV) imaging was taken twice a week, and isocenter shifts were made to match bony anatomy. The difference in position of clips between kV images and digitally reconstructed radiographs was determined. Clips were consist of 3 proximal clips (clip_p, ${\leq}2cm$) and 3 distal clips (clip_d, >2 cm), which were classified according to distance from treatment center. The interfractional displacements of clips were measured in the superior-inferior (SI), anterior-posterior (AP), and right-left (RL) directions. Results: The translocation of clip was well correlated with diaphragm movement in 90.4% (190/210) of all images. The clip position errors greater than 5 mm were observed in 26.0% in SI, 1.8% in AP, and 5.4% in RL directions, respectively. Moreover, the clip position errors greater than 10 mm were observed in 1.9% in SI, 0.2% in AP, and 0.2% in RL directions, despite respiratory control. Conclusion: Quantitative analysis of surgical clip displacement reflect respiratory motion, setup errors and postoperative change of intraabdominal organ position. Furthermore, position of clips is distinguished easily in verification images. The identification of the surgical clip position may lead to a significant improvement in the accuracy of upper abdominal radiation therapy.