• Journal of Environmental Health Sciences
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• v.22 no.4
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• pp.16-24
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• 1996

This study was performed to investigate the effect of co-existence of carbaryl and chlorothalonil on the short-term bioconcentration factor in Carassius auratus(goldfish). The fishes were exposed to the combined treatment of carbaryl and chlorothalonil(0.05 ppm+0.005 ppm, 0.05 ppm+0.010 ppm, 0.10 ppm+0.005 ppm) for 1, 3 and 5 days, respectively. Carbaryl and chlorothalonil in fish and in test water were extracted with n-hexane and acetonitrile. GC-ECD was used to detect and quantitate carbaryl and chlorothalonil. 1-day, 3-day and 5-day bioconcentration factors($BCF_1, BCF_3$ and $BCF_5$) of each pesticide were calculated from the quantitation results. The depuration rate of each pesticide from the whole body of fish was determined over the 72-h period after combined treatment. The results were as follows: $BCF_1$ values of carbaryl were 3.521, 3.802 and 3.587, respectively, when the concentration of carbaryl and chlorothalonil in combined treatment were 0.05+0.005, 0.05+0.010 and 0.10+0.005 ppm. BCF3 values of carbaryl were 4.825, 4.556 and 3.828, respectively, and $BCF_5$ values of carbaryl were 3.974, 3.921 and 4.186, respectively, under the conditions. While $BCF_1$ of chlorothalonil were 0.829, 0.829 and 1.540, respectively, under the same condition of pesticide concentrations $BCF_3$ of chlorothalonil were 2.040, 2.208 and 3.633, respectively, and $BCF_5$ of chlorothalonil were 6.222, 6.667 and 7.095, respectively, under the conditions. Depuration rate constants of carbaryl were 0.022, 0.022 and 0.152, respectively, when the concentration of carbaryl and chlorothalonil in combined treatment were 0.05+0.005, 0.05+0.010 and 0.10+0.005 ppm. While depuration rate constants of chlorothalonil were 0.004, 0.004 and 0.006, respectively, under the same condition of pesticide concentrations. It was observed that no significant differences of carbaryl and chlorothalonil concentration in fish extracts, test water and $BCF_s$ of carbaryl and chlorothalonil between combined treatment and single treatment. It was considered that no appreciable interaction at experimental concentrations was due to low concentrations, 0.005~0.1 ppm. Co-existence of carbaryl and chlorothalonil had no effect on excretion of each pesticide and depuration rate of chlorothalonil was investigated 1/8 slower than that of carbaryl in combined treatment. Therefore, it is considered that the persistence of chlorothalonil in fish body would be higher than that of carbaryl.

• Food Science and Biotechnology
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• v.17 no.5
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• pp.1038-1046
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• 2008

This study was carried out to develop a small-sized biosensor based on surface plasmon resonance (SPR) for the rapid identification of insecticide residues for food safety. The SPR biosensor module consists of a single 770 nm-light emitting diodes (LED) light source, several optical lenses for transferring light, a hemisphere sensor chip, photo detector, A/D converter, power source, and software for signal processing using a computer. Except for the computer, the size and weight of the sensor module are 150 (L)$\times$70 (W)$\times$120 (H) mm and 828 g, respectively. Validation and application procedures were designed to assess refractive index analysis, affinity properties, sensitivity, linearity, limits of detection, and robustness which includes an analysis of baseline stability and reproducibility of ligand immobilization using carbamate (carbofuran and carbaryl) and organophosphate (cadusafos, ethoprofos, and chlorpyrifos) insecticide residues. With direct binding analysis, insecticide residues were detected at less than the minimum 0.01 ppm and analyzed in less than 100 sec with a good linear relationship. Based on these results, we find that the binding interaction with active target groups in enzymes using the miniaturized SPR biosensor could detect low concentrations which satisfy the maximum residue limits for pesticide tolerance in Korea, Japan, and the USA.

• Journal of agricultural medicine and community health
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• v.21 no.2
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• pp.173-193
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• 1996

This study was carried out to elucidate the causes of farmer's disease and greenhouse disease in the rural area of Kyungnam province during 1996. 2,171 (69.1%) of the 3,140 adults above 20 years old who had lived in the selected 20 villages in Uiryong County of Kyungnam Province were selected in order to over both residents who live in the green house distracts and conventional rural area. The results were as follows: 1. Among the subjects, the male was 42.2% and female was 57.6%. The average age for the male was 52.3, 55.6 for the female. 2. The proportion of the farmers in the subjects was 81.5%. Among these 78.0% were engaged in the greenhouse farming. 3. Among the eight symptoms of Nofusho(japanese farmer's comples), lumbago was the most frequently complained and followed by shoulder stiffness and parenthesis of hand or foot. 4. The total score of farmer's disease was evidently higher in the female and the older the score was clearly bigger. 5. The prevalence of Nofusho(Japanese farmer's comples)was 26.8% and 43.15% was for the suspected Nofusho. In the male, the prevalence of Nofusho was 13.6% and 40.9% for the suspected Nofusho and in the female 36.5% and 44.7% were shown, comparatively. 6. According to the multiple classification analysis, sex, age, and occupation were selected as significant variables to explain the total score of Nofusho. 7. The correlations between the total score of Nofusho and the number of sick day, working years, and age were significant, comparatively. 8. According to the multiple classification analysis corolling interaction between independent variables, age was the only variable which was significant in the male and age, pesticide work in the female. 9. The score of greenhouse disease was highest in the group who engaged in greenhouse farming and conventional farming(2.76 for male, 3.77 for female) followed by the group who engaged only in greening house farming(2.66 for male, 3.49 for female) and by the group who engaged only in conventional farming(2.27 for male, 3.05 for female) 10. According to the multiple classification analysis with the total score of greenhouse disease as dependent variable, corolling interaction between independent variables, age and pesticide work were revealed as significant variables in the male, while, pesticide work and farming type were significant in the female. According to the above results, the following could be suggested. Because lumbago, shoulder stiffness, paresthesia of hand and foot were the most frequently complained symptoms in the respondent, the development of farming tool to reduce the body burden and periodical physical exercise and rest is highly recommended. It is revealed that both in the farmer' disease and greenhouse disease the score was higher in the female than in the male. So the reasonables measures is recommended to reduce the working hours of the female. Pesticide work was revealed as the significant variable in the female in farmer's disease and both in the male and the female in greenhouse disease. So the development of the safe method of pesticide spraying including safety education should be introduced. Particularly the female should be excluded in pesticide spraying.

• KSBB Journal
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• v.24 no.3
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• pp.227-238
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• 2009

In the recent years, some organic toxic chemicals were used for obtaining high-yield productivity in agriculture. The undegraded pesticides may remain in the agricultural foods through atmosphere, water, and soil and cause public health problems to environmental resources and human beings even at very low concentrations. Small amounts of pesticides can affect a central nervous system, resulting in immunogenic diseases, infertility problems, respiratory diseases and born marrow diseases, which can lead even to death. Monitoring of the environmental pesticide is one of the important issues for the human well-being. Several kinds of biosensors have been successfully applied to the detection of agrichemical toxicity. Also, few platforms for biocide detection have been definitely developed for the degradation and reaction of pesticides. Biochip and electrochemistry experiments involve immobilizing a receptor molecule on a solid substrate surface, and monitoring its interaction with an analyze in a sample solution. Furthermore, nanotechnology can be applied to make high-throughput analyses that are smaller, faster and sensitive than conventional assays. Some nanomaterials or nanofabricated surfaces can be coupled to biomolecules and used in antibody-based assays and enzymatic methods for pesticide residues. The operation procedure has become more convenient as it does not require labeling procedure. In this paper, we review the recent advances in agrichemical defection research and also describe the label-free biosensor for pesticides using various useful detection methods.

• Korean journal of applied entomology
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• v.44 no.2
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• pp.115-123
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• 2005

Historical changes of population abundances of European red mite (ERM), Panonychus ulmi (Koch), and two-spotted spider mite (TSSM), Tetranychus urticae (Koch) (Acari: Tetranychidae), were described in selected apple orchards in the National Horticultural Research Institute (NHRI, Suwon, Korea), based on research reports of the NHRI from 1958 to 1998. ERM was an abundant species up to 1970, and TSSM became a dominant species after 1980. The change occurred around mid 1970. Three hypotheses were made to explain the change: TSSM competitively replaces ERM, ground cover weeds are a major influencing factor on movement of TSSM (TSSM movement into trees is accelerated by destroying weeds), and ERM and TSSM populations are regulated by natural enemy complexes when the orchard system is not disrupted. And long-term results of the interaction between two species were projected according to the combination of different orchard management strategies: pesticide sprays (non-selective toxic pesticide spray : heavy pesticide pressure (HPP), and selective soft pesticide spray = low pesticide pressure (LPP)) and weed control methods (grass planting, and clean culture system with herbicides). In the HPP and grass planting system, ERMs are abundant because ERM can avoid competition with TSSM as movement of TSSM to trees are restricted, and natural enemy complexes are destroyed by toxic pesticides. In the HPP and clean culture system, TSSMs are abundant because TSSM moves to trees from early season and competitively replaces ERM. In the LPP and grass planting system, ERMs are abundant because movement of TSSM to trees is reduced, but they do not build up a high population density since their densities are regulated by natural enemy complexes. In the LPP and clean culture system, TSSM moves to trees and competes with ERM, but the competition pressure is reduced because population densities of mites are regulated in a lower level by natural enemy complexes. So, ERM can occurs in late season. Thus, two species can coexist temporarily with more ERM in early season and more TSSM in late season. TSSM abundant phenomenon presented in this study can be partially explained as a result of long-term interaction between ERM and TSSM under the HPP and clean culture system.

• Journal of Ecology and Environment
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• v.46 no.2
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• pp.126-135
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• 2022

Background: Pollinators are important ecological elements due to their role in the maintenance of ecosystem health, wild plant reproduction, crop production and food security. The pollinator-plant interaction supports the preservation of plant and animal populations and it also improves the yield in pollination dependent crops. Having knowledge about the plant-pollinator interaction is necessary for development of pesticide risk assessment of pollinators and conservation of endangering species. Results: Traditional methods to discover the relatedness of insects and plants are based on tracing the visiting pollinators by field observations as well as palynology. These methods are time-consuming and needs expert taxonomists to identify different groups of pollinators such as insects or identify flowering plants through palynology. With pace of technology, using molecular methods become popular in identification and classification of organisms. DNA metabarcoding, which is the combination of DNA barcoding and high throughput sequencing, can be applied as an alternative method in identification of mixed origin environmental samples such as pollen loads attached to the body of insects and has been used in DNA-based discovery of plant-pollinator relationship. Conclusions: DNA metabarcoding is practical for plant-pollinator studies, however, lack of reference sequence in online databases, taxonomic resolution, universality of primers are the most crucial limitations. Using multiple molecular markers is preferable due to the limitations of developed universal primers, which improves taxa richness and taxonomic resolution of the studied community.

• The Korean Journal of Pesticide Science
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• v.20 no.2
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• pp.128-132
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• 2016

Volatile organic solvents are used to extract the bioactive materials from raw materials for environmentally-friendly farming materials (EFFM), but the solvent should not remain in EFFM for the safety reasons. Thus qualitative and quantitative analysis method for the solvents using Headspace-GC were evaluated. Water content depleted the detection ratio of hydrophilic solvents and disturbing the hydrophilic interaction with solvents by DMSO might be helped to increase the detection ratio (up to 715%). Surfactant concentration affected to the detection ratio (68.5-179.1%) while surfactant type was not deeply involved the solvent detection. On the other hand, matrix-matched calibration method was accepted the minimum requirements for the quantitative analysis of the solvents in EFFM.

• The Korean Journal of Pesticide Science
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• v.2 no.3
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• pp.64-69
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• 1998

New thirty derivatives of 5,6-dihydro-2-trifluoromethyl-1,4-oxathiin carboxanilide as substrate(S) were synthesized and their fungicidal activities in vivo against rice sheath blight(Rhizoctonia solani) and wheat leaf rust(Puccinia recondita) were examined. The structure activity relationships(SAR) between the activities($pI_{50}$) and a physicochemical parameters of substituents(X) at the phenylcarbamoyl group were analyzed using the adaptive regression analysis method. The 3-methoxy, 11, 3-isopropyloxy, 13 and 3-isopropyl substituent, 25 as X on the phenylcarbamoyl group exhibited the most highest fungicidal activity against the two fungi. The fungicidal potency of the (S) against Puccinia recondita was higher than Rhizoctonia solani. In case of Rhizoctonia solani, the molecular hydrophobicity(${\pi}>0$) and resonance effect(R<0) by meta-alkyl substitutents with electron donating were important factors in determining fungicidal activity. And the HOMO energy(HOMO>0), ABSQ, sum of absolute values of the atomic charges on each atom and specific polarizability(Sp.Pol<0) of (S) were significantly influential towards fungicidal activity against Puccinia recondita.. The interaction between (S) and receptor agonist from the based on SAR studies proceeds through charge-control reaction, and conditions to show higher activity has been also discussed.

• Journal of Environmental Health Sciences
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• v.23 no.2
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• pp.64-71
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• 1997

This study was performed to investigate the effect of co-existence of carbofuran and chlorothalonil on the short-term bioconcentration factor in Brachydanio rerio(zebrafish). The fishes were exposed to the single and combined treatment of carbofuran and chlorothalonil for 1, 3 and 5 days. Experimental concentrations of carbofuran were 0.05 and 0.10 ppm under the single treatment. And those of chlorothalonil were 0.005 and 0.010 ppm. Experimental concentrations of the combined treatment of carbofuran and chlorothalonil were 0.05 ppm+0.005 ppm, 0.05 ppm+0.010 ppm, 0.10 ppm+0.005 ppm for 1, 3 and 5 days, respectively. Carbofuran and chlorothalonil in fish and in test water were extracted with n-hexane and acetonitrile. GC-ECD was used to detect and quantitate carbofuran and chlorothalonil. 1-day, 3-day and 5-day bioconcentration factors(BCF$_1$, BCF$_3$ and BCF$_5$) of each pesticide were obtained from the quantitation results. The depuration rate of each pesticide was determined over the 24-h period after combined treatment. The results were as follows: Carbofuran did not bioaccumulate in zebrafish under the single and combined treatment for testing periods. BCF$_1$ values of chlorothalonil in concentration of 0.005 and 0.010 ppm under the single treatment were 0.508, 0.621, BCF$_3$ were 1.327, 1.511 and BCF$_5$ were 1.331, 1.597, respectively. BCF$_1$ values of chlorothalonil were 0.512, 0.520 and 0.619, respectively, when the concentration of carbofuran and chlorothalonil in combined treatment were 0.05+0.005, 0.05+0.010 and 0.10+0.005 ppm. BCF$_3$ values of chlorothalonil 1.341, 1.338 and 1.513, respectively, and BCF$_5$ values of chlorothalonil were 1.332, 1.327 and 1.521, respectively, under the above combined treatment. Depuration rate constants of chlorothalonil in concentration of 0.005 and 0.010 ppm under the single treatment were 0.011 and 0.012. Depuration rate constants of chlorothalonil were 0.011, 0.010 and 0.011, when the concentration of carbofuran and chlorothalonil in combined treatment were 0.05+0.005, 0.05+0.010 and 0.10+0.005 ppm. It was observed that no significant difference of carbofuran and chlorothalonil concentration in fish extracts, test water, BCFs and depuration rate constants of carbofuran and chlorothalonil between combined treatment and single treatment. It was considered that no appreciable interaction at experimental concentrations due to lower concentrations than LC$_{50}$. It is suggested that the difference of BCFs between carbofuran and chlorothalonil due to those of fat composition of fish and solubility of carbofuran and chlorothaionil.

• Economic and Environmental Geology
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• v.42 no.3
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• pp.217-234
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• 2009

This study characterized groundwater quality and the influence of agrochemicals in a part of Ilgwang-Myeon agricultural area in Gijang-Gun, Busan Metropolitan City, using factor analyses. From the 1st, 2nd, and 3rd analyses of groundwater samples, the mean concentrations of $Ca^{2+}$, $Na^+$, $Mg^{2+}$, $K^+$, $Zn^{2+}$, $Cu^{2+}$, $Fe^{2+}$, $Al^{3+}$, $NO_3\;^-$, $Cl^-$, $SO_4\;^{2-}$, $F^-$, and $SiO_2$ were higher in the 2nd analysis than the other analyses. Pesticide carbofuran and herbicide alachlor were detected at the wells more than a half of all the wells in the 2nd analysis but not in the 4th analysis. This fact may be explained by that a higher precipitation induced higher infiltration rate of contaminants into groundwater during the 2nd survey. According to R- and Q-mode factor analyses, and chemical composition, inorganic constituents excepting $SiO_2$, $HC0_3\;^-$-, and $F^-$ may be influenced by anthropogenic sources (manures, synthetic fertilizers, and domestic wastes), seawater as well as water-mineral interaction. A typical indicator of groundwater contamination, nitrate, exceeds around 4-5 times over the Korean standard for drinking water. Additionally, the influence of seawater diminishes from the seaside to inland.