• 한국균학회지
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• 제40권3호
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• pp.136-140
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• 2012

생강나무에 공생하는 내생균의 다양성을 확인하기 위하여, 강원도 4 개 지역에서 병증이 없는 생강나무 잎을 채집하여 내생균을 분리하였다. 분리된 내생균의 rDNA의 ITS지역을 분석한 결과, Alternaria alternata, Annulohypoxylon annulatum, Creosphaeria sassafras, Diaporthe eres, Discosia sp., Epicoccum nigrum, Glomerella acutata, Glomerella cingulata, Paraconiothyrium brasiliense, Pestalotiopsis neglecta, Phomopsis amygdali, Xylaria sp.의 총 7과 11속 12종이 확인되었으며 Phomopsis amygdali가 모든 연구 지역의 생강나무 잎에서 가장 높은 빈도로 분리되었으며 채집지에 따라 내생균의 종 다양성에 차이가 있음을 확인하였다.

• The Plant Pathology Journal
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• 제24권2호
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• pp.125-130
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• 2008

Various kinds of leaf spots and blights were found in kiwifruit (Actinidia deliciosa) orchards on 2006 in Korea. Disease incidences were quite variable between open-field and rain-proof shelter. Rate of diseased leaves was recorded as about 70% at open-field orchards in late season but use of rain-proof vinyl shelters alleviated the disease incidences by 20%. Angular leaf spots appeared at early infection stage on June and several other symptoms were also recognized as the disease developed afterward. On September, brown leaf blights were the most frequent, followed by grayish brown ring spots, silvering gray leaf blights, zonate leaf blights, dark brown ring spots and angular leaf spots at open-field orchards. Four fungal species were frequently isolated from the disease symptoms. Phomopsis sp. was the most predominant fungus associated with the leaf spot and blight symptoms on kiwifruit, followed by Glomerella cingulata, Alternaria alternata and Pestalo-tiopsis sp. Phomopsis sp. was commonly isolated from angular leaf spots, silvering gray leaf blights, and zonate brown leaf blights. G. cingulata, A. alternata and Pestalotiopsis sp. were isolated from grayish brown ring spots (anthracnose), brown ring spots and zonate dark brown leaf blights. Typical symptoms appeared on the wounded and unwounded leaves, which were inoculated by each of Phomopsis sp., G. cingulata, and Pestalotiopsis sp., but A. alternata caused symptoms only on the wounded leaves.

• 한국버섯학회지
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• 제18권1호
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• pp.72-78
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• 2020

2016년과 2017년 서천, 장흥, 부여, 여주에 소재하는 표고 재배사에서 진균성 병원균을 모니터링 하는 과정에서 Acrodontium crateriforme, Naganishia friedmannii, Pestalotiopsis trachicarpicola, Penicillium wollemiicola, Trichoderma thailandicum 등 5 종의 국내 미기록 진균을 재배사내 실내 공기, 버섯 파리, 버섯 배지재료 등에서 분리하였다. 이들 진균은 PDA 배양하여 생장시키면서 형태적 특성 조사하고 26S rDNA, 28S rDNA, β-TUB 유전자, TEF 유전자 등을 PCR 기술로 증폭하고 그 염기서열 분석을 수행하여 동정하였다. 본 논문에서는 이들의 분류학적 위치와 위해성에 대해 보고하고자 한다.

• 한국균학회지
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• 제44권2호
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• pp.113-117
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• 2016

화악산, 설악산, 함백산에 서식하는 눈측백의 잎에서 내생균을 분리하여 다양성을 조사하였다. 숙주식물로부터 총202개의 균주를 분리하였고, 이 균주들의 ITS rDNA 염기서열을 분석한 결과 32개의 분류군으로 동정되었으며, Diaporthe nobilis, Mycosphaerella handelii, Pestalotiopsis cocculi 3종이 국내 미기록종으로 확인되었다. 분리된 균주 중 61.5%는 Dothideomycetes였으며, 27.0%는 Sordariomyetes, 11.5%는 Leotiomycetes에 속하였다. 눈측백에 서식하는 내생균에서 우점종은 Dothideomycetes에 속하는 Phyllosticta spinarum인 것으로 조사되었으며 이 종에 대한 후속연구가 필요한 것으로 생각된다.

• 한국균학회지
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• 제45권4호
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• pp.292-303
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• 2017

2016년 버섯 재배사 내 실내공기, 식물병반(동백나무의 잎, 줄딸기, 은방울꽃, 생강나무), 버섯 배양배지 재료, 오염된 가구 등에서 진균을 분리 동정하였다. 동정된 진균 중 18종이 국내 미기록임을 확인하였다. 동정된 진균은 버섯 재배사 내 실내공기에서 6종, 식물병반에서 6종, 버섯 배양배지 재료에서 5종, 오염된 가구에서 1종이 각각 분리되었다 이들 동정된 균주에 대한 형태학적 특성과 ITS rDNA, 18S rDNA, 28S rDNA, calmodulin gene, translation elongation factor gene, ${\beta}-tubulin$ gene 등의 염기서열에 기반한 분자계통학적 관계에 대하여 기술하였다.

• Mycobiology
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• 제46권2호
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• pp.85-91
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• 2018

Endophytic fungi strains (n = 81) were isolated from the leaves, barks, and fruits of Camellia oleifera from Hunan province (China) to delineate their species composition and potential as biological control agents of C. oleifera anthracnose. The fungi were identified by morphological and phylogenetic analyses. Fungal colonization rates of the leaves, barks, and fruits were 58.02, 27.16, and 14.81%, respectively. The isolates were identified as 14 genera, belonging to two subdivisions, Deuteromycotina and Ascomycotina; 87.65% of all isolates belonged to Deuteromycotina. The dominant species, occurring with a high relative frequency, were Pestalotiopsis sp. (14.81%), Penicillium sp. (14.81%), and Fusarium sp. (12.35%). The Simpson's and Shannon's diversity indices revealed the highest species diversity in the leaves, followed by the barks and fruits. The similarity index for the leaves versus barks comparison was the highest, indicating that the number of endophytic fungal species shared by the leaves and barks was higher than barks and fruits or leaves and fruits. Based on the results of dual culture experiments, only five strains exhibited antifungal activity against C. oleifera anthracnose pathogen, with isolate ty-64 (Oidium sp.) generating the broadest inhibition zones. Our results indicate that the endophytes associated with C. oleifera could be employed as natural agents controlling C. oleifera anthracnose.

• The Plant Pathology Journal
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• 제22권4호
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• pp.381-385
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• 2006

Antifungal activity of Korean and Chinese lichen-forming fungi(LFF) was evaluated against plant pathogenic fungi of Botryosphaeria dothidea, Botrytis cinerea, Diaporthe actinidiae, Pestalotiopsis longiseta, Pythium sp., Rhizoctonia solani, and Sclerotium cepivorum. The LFF were isolated from Cladonia scabriuscula, Melanelia sp., Nephromopsis asahinae, Nephromopsis pallescens, Parmelia laevior, Pertusaria sp., Ramalina conduplicans, Ramalina sinensis, Ramalina sp., Umbilicaria proboscidea and Vulpicida sp. with discharged spore method. The isolates were deposited in the herbarium of Korean Lichen Research Institute(KoLRI) in Sunchon National University. The LFF of Melanelia sp., P. laevior, Pertusaria sp., R. conduplican and Ramalina sp. exhibited strong antifungal activity against all of the pathogenic fungi examined. Among them, LFF of P. laevior showed more than 90% of inhibition in fungal hyphae growth, compared with control. The results imply that LFF can be served as a promising bioresource to develop novel biofungicides. Mass cultivation of the LFF is now under progress in laboratory conditions for chemical identification of antifungal substances.

• Journal of the Korean Wood Science and Technology
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• 제47권4호
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• pp.459-471
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• 2019

Several species of Aquilaria and Gyrinops are native to Indonesia and well known as agarwood-producing trees with a high economic value. Their bioactive compounds have a wide spectrum of uses, such as in medicine and cosmetics. These genera have undergone extensive search for novel bioactive compounds. The purpose of this study was to isolate, identify, and characterize the endophytic fungi community associated with Aquilaria malaccensis, A. microcarpa, Gyrinops versteegii, and A. crassna trees and investigate their bioactive properties as antioxidant agents and antagonists. A total of 50 fungi were successfully isolated from different tissues of the four species of agarwood-producing trees. Two isolates exhibited strong antioxidant activity, namely, Apodus oryzae (R2MC3A, $IC_{50}$ 60.92 mg/mL) and Diaporthe sp. (P1DS1[C], $IC_{50}$ 76.65 mg/mL). Two isolates, Pestalotiopsis theae (P3BS3[B]) and Curvularia sp. (P2CD3A), showed >75% antifungal activity against pathogenic Fusarium solani. The results revealed that endophytic fungi associated with the studied agarwood-producing trees had potential antioxidant and antifungal activities for further applications in biotechnology.