• Journal of Plant Biotechnology
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• 제49권3호
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• pp.193-206
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• 2022

Astringent persimmon (Diospyros kaki Thunb.) is an important fruit crop in Korea; it possesses significant medicinal potential. However, knowledge regarding the pathogens affecting this crop, particularly, viruses and viroids, is limited. In the present study, reverse transcription-polymerase chain reaction (RT-PCR) and high-throughput transcriptome sequencing (HTS) were used to investigate the viruses and viroids infecting astringent persimmons cultivated in Korea. A one-step multiplex RT-PCR (mRT-PCR) method for the simultaneous detection of the pathogens was developed by designing species-specific primers and selecting the primer pairs via combination and detection limit testing. Seven of the sixteen cultivars tested were found to be infection-free. The RT-PCR and HTS analyses identified two viruses and one viroid in the infected samples (n = 51/100 samples collected from 16 cultivars). The incidence of single infections (n = 39/51) was higher than that of mixed infections (n = 12/51); the infection rate of the Persimmon cryptic virus was the highest (n = 31/39). Comparison of the monoplex and mRT-PCR results using randomly selected samples confirmed the efficiency of mRT-PCR for the identification of pathogens. Collectively, the present study provides useful resources for developing disease-free seedlings; further, the developed mRT-PCR method can be extended to investigate pathogens in other woody plants.

• 식물병연구
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• 제14권3호
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• pp.226-228
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• 2008

2007년 경북문경의 옥수수 재배지 약 $1000\;m^2$에 RBSDV가 약 $99{\sim}100%$ 발생되었다. 이 포장은 바로 인접지역에 감나무가 재배되고 있었으며 이 나무 아래에 녹비작물로서 보리를 재배하였다. 보리는 RBSDV의 매개충인 애멸구의 겨울철 중간기주로 작용하여 애멸구의 월동에 유리한 환경으로 작용한 것으로 생각된다. 진단은 S9 특이 primer를 사용한 RT-PCR 방법으로 확인하였다. 이 포장에서 매개충인 애멸구를 채집하여 RT-PCR에 의한 보독충율을 조사하였다. 5월 3일, 6월 7일, 8월 4일 3회 애멸구를 채집하여 검정한 결과 각각 2.9%, 4.8%, 4.4%의 보독충율을 나타내었다. 이것은 RBSDV에 이병된 벼 포장에서 나타나는 보독충율과 비슷한 결과를 나타내었다.