• 대한통합의학회지
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• 제8권4호
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• pp.163-169
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• 2020

Purpose : This study aimed to investigate the anti-allergic effect of Persicaria perfoliata water extract (PPWE) on IgE stimulated rat basophilic leukemia (RBL-2H3) cell line. Methods : P. perfoliata (L.) H. Gross has been used in traditional medicine as an anti-allergic agent, antipyretic, and diuretic and for respiratory disorders. To analyze the anti-allergic activity of PPWE, release of β-hexosaminidase in RBL-2H3 cells was estimated by enzyme linked immunosorbant assay (ELISA). Also, the cytotoxic effect of PPWE was identified by WST assay, and nuclear factor (NF)-κB and its upstream signaling molecules were assessed by western blot analysis. Results : PPWE treatment significantly attenuated β-hexosaminidase release in a dose dependent manner without any cytotoxicity. PPWE inhibited β-hexosaminidase activity by 38.4±1.2, 36.6±0.6, 32.5±2.2 and 26.5±1.2 at 500, 250, 100, and 50 ㎍/㎖ of PPWE, respectively, compared with the control group. In addition, an analysis of the expression level of NF-κB, an inflammation transcription factor, in RBL-2H3 cells upon IgE stimulation provided reults consistent with the results of β-hexosaminidase release. The phosphorylated status of upstream signaling molecules for transcription factor, mitogen activated protein kinases (MAPKs), was also analyzed. The results showed that PPWE treatment dose-dependently inhibited phosphorylation of extracellular regulatory kinase (ERK) and c-Jun N-terminal kinase (JNK). These results show that PPWE had a strong IgE-mediated degranulation inhibitory effect on RBL-2H3 cells. Conclusion : P. perfoliata ameliorated IgE-mediated allergic reaction via the modulation of MAPK and NF-κB signaling pathway in RBL-2H3 cells. These results indicate that P. perfoliata could be a potential candidate for a treatment strategy against various allergic disorders.

• 동의생리병리학회지
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• 제23권2호
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• pp.389-396
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• 2009

The ethanol extract of Persicaria perfoliata (EPP) induced relaxation of the phenylephrine-precontracted aorta in a dose-dependent manner, which was abolished by removal of functional endothelium. Pretreatment of the aortic tissues with NG-nitro-L-arginine methyl ester (L-NAME) or 1H-[1,2,4}-oxadiazole-[4,3-${\alpha}$)-quinixalin-1-one (ODQ) inhibited the relaxation induced by EPP. However, EPP-induced relaxation was not blocked by pretreatment with indomethacine, glibenclamide, tetraethylammonium (TEA), atropine, or propranolol. Incubation of endothelium-intact thoracic aortic ring with EPP increased the production of cGMP, which was also blocked by pretreatment with L-NAME or ODQ. These results suggest that EPP dilates vascular smooth muscle via endothelium-dependent NO/cGMP signaling.

• 한국습지학회지
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• 제5권2호
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• pp.43-55
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• 2003

Vegetation structure was investigated in the Hyeonchang wetland and its watershed around the Nakdong-gang, Changryeong-county, Gyeongsangnamdo, Korea. from May to August, 2001. The vegetation type of the Hyeonchang wetland was classified into 12 communities based on the actual vegetation map: Phragmites communis community, Zizania latifolia community, Phragmites communis - Persicaria perfoliata community, Salix koreensis community, Persicaria perfoliata - Phragmites communis community, Spirodela polyrhiza community, Persicaria perfoliata community, Cyperus amuricus community, Cyperus amuricus-EchinochJoa crus-galli var. frumentacea community, Phragmites communis-Zizania latifolia community, EchinochJoa crus-galli var. frumentacea community, and Persicaria maackiana community. Among them, Phragmites communis community was the largest (4.3 ha, 24%). The dominant vegetation type were Phragmites communis community, Echinochloa crus-galli var. frumentacea-Persicaria maackiana community, and Cyperus amuricus subcommunity based on the phytosociological method. The vegetation type of the Hyeonchang wetland watershed was classified into five communities based on the actual vegetation map: Pinus densilflora community, Pinus rigida community, Pinus densiflora-Quercus acutissima community, Pinus densiflora-Larix gmelini var. principis-ruprechtii community, and Populus tomentiglandulosa community. Among them, Distribution area of Pinus densiflora community was largest (399.3 ha, 61.8%). And the degree of green naturality of the Pinus densiflora community was 7 and 8 degree.

• 생명과학회지
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• 제25권4호
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• pp.390-396
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• 2015

본 연구는 항산화 기능이 뛰어나다고 알려진 며느리배꼽(Persicaria perfoliata)의 캘러스를 사용하여, 어떤 식물호르몬이 부정근 형성에 영향을 미치는지 알아보기 위한 실험이다. 며느리배꼽의 잎으로부터 캘러스를 유도하고 이로부터 부정근 형성을 관찰하였다. 캘러스 유도의 최적 조건을 구한 결과 1% sucrose, 4.5 μM 2.4-D, 1/2 MS였다. 어떤 식물호르몬이 이 식물의 캘러스에서 부정근 형성 효과를 가지고 있는지 알아보기 위하여 GA₃, IAA, 2iP, 2,4-D를 캘러스 조직에 각각 첨가한 결과, GA₃와 IAA를 첨가한 배지에서만 부정근이 나타났음을 확인 할 수 있었다. 이를 자세히 조사하기 위해서 GA₃와 IAA의 농도별(0.1, 1, 10 mg/l) 부정근 형성, 길이 및 직경을 알아본 결과, 높은 수준의 GA₃ 또는 낮은 수준의 IAA처리에서 더 많은 부정근 형성을 보였다. 이 두 호르몬 중 어떤 것이 더욱 더 중요한 역할을 하는지 알아보기 위하여 옥신 유입 저해제인 NPA와 지베렐린 생합성 저해제인 PBZ를 처리한 실험을 한 결과, GA₃가 IAA 보다 부정근 형성에 더욱 더 중요한 역할이라는 것을 알았다. 하여 지베렐린의 수준을 증가 또는 감소시킨다고 알려진 식물호르몬(IAA, 2iP, kinetin, ABA)들을 GA₃와 혼용 처리한 결과, 이 역시 지베렐린이 부정근을 형성하는데 중요한 역할을 한다는 것을 재확인 하였다. 본 연구는 지베렐린이 며느리배꼽의 캘러스에서 부정근 형성을 증진시킨다는 것을 처음으로 밝힌다.

• 한국미생물·생명공학회지
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• 제38권3호
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• pp.278-282
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• 2010

1) 며느리배꼽의 항균활성 측정결과, S. aureus, P. acnes, 에 대한 ethyl acetate 분획의 MIC는 각각 0.25%, 0.25%로 큰 항균활성을 나타내었으며, 천연 방부제, 항균제로서 역할이 충분히 기대된다. 2) 며느리배꼽 추출물의 ethyl acetate 분획의 TLC는 5개의 띠(PP1, PP2, PP3, PP4, PP5)로 분리가 되었고, 그 중에서 농도가 가장 진한 것은 $R_f$ 값이 0.32인 PP4로 나타났다. 3) 며느리배꼽 추출물 중 ethyl acetate 분획의 HPLC 크로마토그램은 검출파장 320nm에서 8개의 peak를 나타내었고, peak 1은 caffeic acid, peak 2는 quercetin-3-o-glucuronide, peak 3은 isoquercitrin, peak 5는 quercetin, peak 6는 apigenin으로, peak 7은 kaempferol 임을 확인하였다. 4) 며느리배꼽 추출물의 aglycone 분획의 TLC는 3개의 띠(PPA1, PPA2, PPA3)로 분리가 되었고, 그 중에서 농도가 가장 진한 것은 $R_f$ 값이 0.45인 PPA3로 나타났다. 5) 며느리배꼽 추출물 중 aglycone 분획의 HPLC 크로마토그램은 검출파장 260 nm에서 3개의 peak를 나타내었고, peak 1은 caffeic acid, 높이가 가장 높은 peak 2는 quercetin 그리고 peak 3은 kaempferol임을 확인하였다. 이상의 결과들로부터 며느리배꼽 추출물의 강력한 항산화 성분과 항균 활성으로부터 며느리배꼽 추출물의 항산화, 항균 화장품으로의 응용 가능성을 시사한다.

• 대한통합의학회지
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• 제10권3호
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• pp.27-36
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• 2022

Purpose : This study was aimed to analyze the effects of cosmetics containing Persicaria perfoliata water extract (PPWE) on the skin moisturizing and improvement of skin condition in clinical trials. Methods : Clinical trial was conducted for five weeks after IRB approval at Dong-Eui University. Out of a total of 64 people, 15 people each were assigned to four groups as follows; control group A, B, C and the experimental group A that using cosmetic containing PPWE. Skin condition was measured two times, before and after clinical trial, by a professional skin analyzer, SDM (skin diagnosis system). Moisture and oil value of participants was analyzed twice, each morning and evening, using a portable device on their cheeks. In addition, the survey was investigated subjective satisfaction on change in skin condition and the satisfaction on the use of cosmetics. Result : The experimental group exhibited subjectively significant changes before and after clinical trials on skin its dryness (p=.039), blush (p=.017), and redness (p<.001). In addition, subjective evaluation was also the highest satisfaction in aspects of number of application (p=.003), amount of application (p=.002), moisture maintenance, and skin scratching frequency. The satisfaction on the use of cosmetics was the highest in the intention to repurchase (p=.045), recommendation willingness to others (p=.020), and intention to use various products (p=.001). Skin moisture of the clinical trial participants using the SDM, moisture level and elasticity of the experimental group increased by 12.94 and 10.28. Moisture level, which was measured by a portable device, was the most potently increased in the experimental group. Conclusion : Consequently, PPWE containg cosmetics exhibited the effects of moisturization and attenuated skin dryness in clinical trials, which might be utilized as a fundamental data to develop numerous lines of cosmetics.

• 대한화장품학회지
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• 제35권3호
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• pp.235-241
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• 2009

본 연구에서는 며느리배꼽 추출물들의 항산화 효능 및 tyrosinase, elastase 저해 효과에 관한 조사를 수행하였다. 며느리배꼽 추출물의 free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) 소거활성($FSC_{50}$)은 aglycon 분획($12.38{\mu}g$/mL)에서 가장 큰 활성을 나타내었고, 또한 luminol-의존성 화학발광법을 이용한 $Fe^{3+}$-EDTA/$H_2O_2$ 계에서 생성된 활성산소종(reactive oxygen species, ROS)에 대한 며느리배꼽 추출물의 총항산화능은 추출물의 ethyl acetate 분획($0.35{\mu}g$/mL)에서 가장 큰 활성을 나타내었다. 며느리배꼽 추출물에 대하여 광증감제인 rose-bengal로 증감된 사람 적혈구의 광용혈에 대한 억제 효과를 측정하였을 때, 추출물의 aglycon 분획을 제외하고 다른 추출물들은 농도 의존적($1{\sim}50{\mu}g$/mL)으로 세포보호 효과를 나타내었다. Tyrosinase의 활성 저해 효과($IC_{50}$)는 며느리배꼽 추출물의 ethyl acetate 분획과 aglycon 분획이 각각 $136.00{\mu}g$/mL, $68.10{\mu}g$/mL으로 나타났으며, elastase의 활성 저해 효과($IC_{50}$)는 ethyl acetate 분획과 aglycon 분획이 각각 $67.20{\mu}g$/mL, $43.50{\mu}g$/mL으로 나타났다. 이상의 며느리배꼽 추출물이 $^1O_2$ 혹은 다른 ROS를 소광시키거나 소거함으로써 그리고 ROS에 대항하여 세포막을 보호함으로써 생체계, 특히 태양 자외선에 노출된 피부에서 항산화제로서 작용할 수 있음을 가리키며, 며느리배꼽 추출물의 tyrosinase 및 elastase 저해 활성으로 부터 항산화, 항노화 화장품 소재로서의 응용 가능성을 확인하였다.

• 한국잡초학회지
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• 제10권2호
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• pp.67-74
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• 1990

1989년(年)9월(月) 낙농강유역(洛東江流域) 38지점(地點)에서 총(總) 29과(科) 102종(種)의 잡초(雜草)가 조사(調査)되었다. 그 가운데 일년생(一年生) 광엽잡초(廣葉雜草)가 54%, 다년생(多年生) 광엽잡초(廣葉雜草)가 21%로 광엽잡초(廣葉雜草)가 전체(全體)의 72%를 점유(占有)하였다. 2차원적(次元的) 분류방법(分類方法)으로 환삼덩쿨(Humulus japonicum)군락(群落), 여뀌(Persicaria hydropiper)군락(群落), 고마리(Persicaria thunbergii)군락(群落), 사철쑥(Artemisia capillaris)군락(群落), 개여뀌 - 강아지풀 - 바랭이(Persicaria blumei - Setaria viridis - Digitaria sanguinalis)군락(群落), 갈대(Phragmites communis)군락(群落)이 조사(調査)되었다. 가장 큰 군락(群落)은 갈대 군락(群落)이었고, 총(總) 38 조사지점중(調査地點中) 15지점(地點)에서 갈대가 발생(發生)되었고 고마리, 환삼덩쿨, 개여뀌 등(等)의 잡초(雜草)들이 우점발생(優點發生)하였다.

• 한국자원식물학회지
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• 제24권1호
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• pp.1-9
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• 2011

자생식물의 항산화 활성 탐색을 위하여 140종의 식물을 80% ethanol로 추출하여 활성을 확인하였다. 그 결과 며느리배꼽이 가장 높은 항산화 활성을 나타내었고(VitaminE, ORACPE=1.0), 쉬땅나무, 찔레나무, 좁쌀풀, 갈참나무, 신갈나무 그리고 싸리의 순으로 항산화활성이 높은 것으로 조사되었다(ORACPE ${\geq}$ 1.50). 또한 80% 에탄올 추출물의 용매별(n-hexane, $CH_2Cl_2$, EtOAc, n-BuOH, water) 분획층 ORAC 결과에서도 며느리배꼽과 쉬땅나무가 각각 $1.13{\pm}0.002$, $1.10{\pm}0.004$, $3.67{\pm}0.232$, $1.62{\pm}0.049$, $1.05{\pm}0.003$; $1.02{\pm}0.007$, $v1.00{\pm}0.016$, $2.94{\pm}0.130$, $1.80{\pm}0.019$ 그리고 $1.04{\pm}0.012$로 높은 값을 나타내었다. 그 중 항산화 활성이 가장 높은 EtOAc 분획층의 유효성분 규명 등의 추가 연구가 필요하다.

• 대한통합의학회지
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• 제12권1호
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• pp.63-71
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• 2024

Purpose : Skin is the primary barrier to protect the body from various exogenous factors. Among them, UVB exposure can cause the induction of not only excessive inflammatory responses but also the degradation of extracellular matrix (ECM), including collagen and elastin. This study tried to investigate the ameliorative effect of Persicaria perfoliata ethanol extract (PPEE) on UVB-irradiated photodamage through the regulation of activator protein (AP)-1, phosphoinositide 3-kinase (PI3K)/Akt, and mitogen-activated protein kinase (MAPK) signaling molecules in HaCaT cells. Methods : The cytotoxicity of PPEE on HaCaT cells was evaluated by the WST-1 assay. The 80 mJ/cm² of UVB (312 nm) was irradiated on HaCaT cells to induce the photodamage. Western blot analysis was conducted to investigate the protein expression levels of cyclooxygenase (COX)-2, matrix metalloproteinase (MMP)-9, and heme oxygenase (HO)-1 for ameliorative status by PPEE treatment in UVB-exposed HaCaT cells. In addition, the activated status of the inflammatory transcription factor, AP-1, as well as upstream signaling molecules, PI3K/Akt, and MAPK, were also evaluated by Western blot analysis. Results : Any cytotoxic effect was not induced at the concentration up to 200 ㎍/ml by PPEE treatment. Protein expression levels of COX-2 and MMP-9 were significantly down- and up-regulated by PPEE treatment. The inflammatory transcription factor AP-1, stimulated by UVB irradiation, was also significantly attenuated by PPEE treatment. The phosphorylated status of PI3K/Akt and MAPK were mitigated by PPEE treatment in UVB-exposed HaCaT cells. Moreover, PPEE treatment potently accelerated the expression of HO-1 and its transcription factor, nuclear factor-erythroid 2-related factor (Nrf)2, which is known for its anti-inflammatory activity. Conclusion : Consequently, PPEE treatment significantly regulated COX-2 and MMP-9 expressions in UVB-irradiated HaCaT cells. The inflammatory transcription factor AP-1, along with upstream signaling molecules PI3K/Akt and MAPKs, were also attenuated by PPEE treatment in UVB-exposed HaCaT cells. Additionally, PPEE treatment exaggerated HO-1 expression and Nrf2 activation, which might have contributed to the anti-inflammatory activity of PPEE. These results indicate that PPEE could be a candidate for attenuating UVB-induced photodamage in human skin.