• 대한수학교육학회지:수학교육학연구
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• 제11권1호
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• pp.11-35
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• 2001

Many teachers report familiarity with and adherence to reform ideas, but their actual teaching practices do not reflect a deep understanding of reform. Given the challenges in implementing reform, this study intended to explore the breakdown that may occur between teachers' adoption of reform objectives and their successful incorporation of reform ideals. To this end, this study compared and contrasted the classroom social norms and sociomathematical norms of two United States second-grade teachers who aspired to implement reform. This study is an exploratory, qualitative, comparative case study. This study uses the grounded theory methodology based on the constant comparative analysis for which the primary data sources were classroom video recordings and transcripts. The two classrooms established similar social norms including an open and permissive learning environment, stressing group cooperation, employing enjoyable activity formats for students, and orchestrating individual or small group session followed by whole group discussion. Despite these similar social participation structures, the two classes were remarkably different in terms of sociomathematical norms. In one class, the students were involved in mathematical processes by which being accurate or automatic was evaluated as a more important contribution to the classroom community than being insightful or creative. In the other class, the students were continually engaged in significant mathematical processes by which they could develop an appreciation of characteristically mathematical ways of thinking, communi-eating, arguing, proving, and valuing. It was apparent from this study that sociomathematical norms are an important construct reflecting the quality of students' mathematical engagement and anticipating their conceptual learning opportunities. A re-theorization of sociomathematical norms was offered so as to highlight the importance of this construct in the analysis of reform-oriented classrooms.

• 공학교육연구
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• 제14권1호
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• pp.40-45
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• 2011

본 연구는 협동학습에서 팀 프로젝트를 수행하는 공대생들을 대상으로 창의적 문제해결스타일 및 갈등관리유형이 팀 수행 성적과 어떤 관련성이 있는 지를 파악함으로써 공대생들의 문제해결능력과 갈등관리를 위한 커뮤니케이션능력 증진프로그램을 구성하는데 도움이 되는 기초정보를 제공하기 위해 수행되었다. 팀 수행 성적이 상, 하위 30%에 해당하는 집단 간에 창의적 문제해결스타일의 두 가지 차원(변화에 대한 지향, 의사결정의 중심)에서 유의미한 차이가 나타났다. 상위권은 대개 개량스타일(76.7%)과 과제중심스타일(65.1%)에 해당된 반면, 하위권은 혁신스타일(72.2%)과 사람중심스타일(86.1%)에 해당되었다. 갈등관리유형의 경우, 상위권은 경쟁논리형(22.8%)과 순응타협형(17.7%), 하위권은 순응타협형(20.3%)과 방임체념형(12.7%)이 가장 많았다. 마지막으로 본 연구의 제한점과 후속연구를 위한 제언이 논의되었다.

• The Journal of Korean Physical Therapy
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• 제9권1호
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• pp.59-70
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• 1997

The ability of neurotropic alpha herpesviruses to replicate within synaptically linked neurons has made these pathogens valuable tools for transneuronal analysis. Recent studies suggest that unique gene products expressed by genetically engineered strains of virus may permit the use of multiple strains in complex tracing paradigms. In the present study we have examined the invasiveness of two genetically engineered strains of the swine pathogen known as pseudorabies virus(PRV). The two strains were isogenic with the attenuated Bartha strain of PRV; in one strain a lacZ reporter gene was inserted into the gC locus (PRV-BaBlu; $4.75\times10^8pfu/ml$) contrained a PRV envelope glycoprotein gene that was absent in PRV-BaBlu. Simultaneous or temporally separated sequential injection of $4\mu\ell$ of each strain into the ventral wall of the stomach produced a predictale course of retrograde synaptic infection. The results were as follows: 1. PRV-BaBlu and PRV-D infected the dorsal motor nucleus of vagus nerve(DMV) and paraventricular nucleus(PVN). 2. Invasion and replication of PRV-D occured at a faster rate than the parental strain or PRV-BaBlu. 3. PRV-D was much more virulent than PRV-BaBlu or the parental strain. 4. Co-injection of PRV-D and PRV-BaBlu produced an infection that was more virulent than that produced by the parental strain (PRV-Bartha), 5. Neurons in DMV were permissive to co-infection with PRV-D and PRV-BaBlu when they were injected simultaneously into the same site. 6. Replication of PRV-BaBlu was compromised by prior infection of the same circuit with PRV-D. 7. Prior infection of neurons with PRV-D maked them resistant to infection with PRV-BaBlu.

• 대한의생명과학회지
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• 제12권1호
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• pp.17-22
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• 2006

The baculovirus expression system is a powerful method for producing large amounts of the human erythrocyte-type glucose transport protein, heterologously. Characterization of the expressed protein is expected to show its ability to transport sugars directly. To achieve this, it is a prerequisite to know the properties of the endogenous sugar transport system in Spodoptera frugiperda Clone 21 (Sf21) cells, which are commonly employed as a host permissive cell line to support the baculovirus replication. The Sf21 cells can grow well on TC-100 medium that contains 0.1% D-glucose as the major carbon source, strongly suggesting the presence of endogenous glucose transport system. However, unlike the human glucose transport protein that has a broad substrate and inhibitor specificity, very little is known about the nature of the endogenous sugar transport system in Sf21 cells. In order to characterize further the inhibitor recognition properties of the Sf21 cell transporter, the ability of phloretin, cytochalasin B and D-fructose to inhibit 2-deoxy-D-glucose (2dGlc) transport was examined by measuring inhibition constants $(K_i)$. The $K_i's$ for reversible inhibitors were determined from plots of uptake versus inhibitor concentration. The 2dGlc transport in the Sf21 cells was very potently inhibited by phloretin, the aglucone of phlorizin with a $K_i$ similar to the value of about $2{\mu}M$ reported for inhibition of glucose transport in human erythrocytes. However, the Sf21 cell transport system was found to differ from the human transport protein in being much less sensitive to inhibition by cytochalasin B (apparent $K_i$ approximately $10\;{\mu}M$). In contrast, It is reported that the inhibitor binds the human erythrocyte counterpart with a $K_d$ of approximately $0.12\;{\mu}M$. Interestingly, the Sf21 glucose transport system also appeared to have high affinity for D-fructose with a $K_i$ of approximately 5mM, contrasting the reported $K_m$ of the human erythrocyte transport protein for the ketose of 1.5M.

• 생물정신의학
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• 제7권2호
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• pp.115-122
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• 2000

The development of molecular biology has brought many changes in psychiatry. Molecular biology makes us possible to know the cause of mental disorders that provide the way to prevent the disorders, and to develop various accurate diagnostic and treatment methods for mental disorders. The author discusses the concept, cause, and treatment of mental disorders in the aspect of molecular biology. Importing the methods of molecular biology into psychiatry, we can anticipate to get a number of the goals of psychiatric genetics, including identification of specific susceptibility genes, clarification of the pathophysiological processes whereby these genes lead to symptoms, establishment of epigenetic factors that interact with these genes to produce disease, validation of nosological boundaries that more closely reflect the actions of these genes, and development of effective preventive and therapeutic interventions based on genetic counseling, gene therapy, and modification of permissive or protective environmental influences. In addition to their capacity to accelerate the discovery of new molecules participating in the nervous system's response to disease or to self-administered drugs, molecular biological strategies can also be used to determine how critical a particular gene product may be in mediating a cellular event with behavioral importance. Molecular biology probably enables us discover the environmental factors of mental disorders and allow rational drug design and gene therapies for mental disorders, by isolation of gene products that facilitate a basic understanding of the pathogenesis of these disorders. A specific genetic linkage may suggest a novel class of drugs that has not yet been tried. With respect to gene therapy, the hypothetical method would use a gene delivery system, most likely a modified virus, to insert a functional copy of a mutant gene into those brain cells that require the gene for normal function.

• 한국미생물·생명공학회지
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• 제13권1호
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• pp.41-49
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• 1985

Bacillus sphaericss 1593균주를 ultra violet light (366nm, 253.7nm)와 dimethyl sulfate으로 처리하여 다수의 감온성 돌연변이체를 분리하여 이중 25균주를 선택하였고 24개의 감온성 돌연변이체는 $42^{\circ}C$의 제한온도에서 전혀 성장을 하지 않았으며, 9개의 감온성 돌연변이체는 제한온도 $42^{\circ}C$에서 허용온도의 성장과 비교하여 1/2정도의 감소를 보였다. 기타 2개의 감온성 돌연변이체는 저온 민감성인 감온성 돌연변이체로서 허용온도($30^{\circ}C$)에서의 성장이 제한온도($42^{\circ}C$) 성장보다 감소를 보였다. 이러한 결과에 따라서 감온성 돌연변이체를 치사군과 비치사군 및 저온민감성 감온성 돌연변이체로 분리할 수 있었다. 또한 치사군의 제한온도에서의 발육은 고체배지 및 액체배지 양쪽에서 성장을 하지 않는 반면 비치사군들의 돌연변이체들은 제한온도 $42^{\circ}C$에서 고체배지 및 액체배지의 성장은 허용온도에서 배양한 결과보다 저조한 성장을 나타내었다. 감온성 돌연변이체들은 $42^{\circ}C$에서 12시간 배양한 후 허용온도인 $30^{\circ}C$ 액체배지로 이식하여 배양한 결과 치사군중 ts-U1112, ts-D1290, ts-D1342, ts-D1504 돌연변이체들은 성장을 계속하지 않았으며, ts-U351, ts-U828, ts-U892, ts-D1350, ts-D1479, ts-D2601과 ts-D2801 돌연변이체들은 성장이 계속됨을 관찰할 수 있었다.

• 대한약리학회지
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• 제19권2호
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• pp.1-8
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• 1983

Prostaglandin(PG) is ubiquitously distributed in most mammalian tissue and their actions are complicated. Especially in autonomic nervous system, there are evidences indicating that PGs act as neuromodulators i.e., PGs, which are released in the vicinity of autonomic neuroeffector junctions, influence the release and the response of the neurotransmitter. Present study was undertaken to elucidate the interrelationship between $PGF_{2\alpha}$ and adrenergic ${\alpha}_2-receptor$ function in electrical field stimulation induced contractile response of vas deferens in rat. Male rat, weighing 150{\sim}200\;g, was sacrificed and vas deferens was obtained. The isolated vas deferens strip was placed between two platinum electrodes in temperature controlled $(37^{\circ}C)$ muscle chamber containing Tyrode's solution and the electrical field stimulation(EFS) induced contraction was recorded with Grass Polygraph(Model 7) via force displacement transducer (FT .03, Grass). The results are summarized as follows: 1) Electrical field stimulation for 1sec( 1 msec, 40 cps) induced contraction of vas deferens was completely blocked by tetrodotoxin. 2) Bretylium caused marked inhibition of the EFS-induced contraction, hut tyramine and cocaine augmented the contraction. 3) EFS-induced contraction was inhibited or little affected in distal portion of vas deferens by norepinephrine or methoxamine, but the contraction was rather augmented by the ${\alpha}-agonists$ in proximal portion. 4) Clonidine inhibited the EFS-induced contraction proportionally to the concentration in distal portion, which was blocked by yohimbine pretreatment, but in the presence of $PGF_{2\alpha}$ the blockade by yohimbine was reversed. 5) Indomethacin pretreatment reduced the effect of clonidine, but addition of $PGF_{2\alpha}$ after washing-out the indomethacin caused the contraction to the control level. From these results it is suggested that PG synthesis is a necessary step and the PG itself has a permissive role in ${\alpha}_2-adrenoceptor$ action in rat vas deferens.

• 한국ITS학회 논문지
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• 제10권2호
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• pp.35-41
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• 2011

경찰청에서는 국내 교통문제를 해결하고 국제적 교통운영체계를 수립하기 위하여 "교통운영체계 선진화 방안"사업을 추진하고 있다. 본 연구는 교통운영체계 선진화 방안의 추진 효과를 분석하였으며, 그 결과 교통소통 및 교통안전측면에서 효과가 있는 것을 밝혔다. 그러나 정책에 대한 시민들의 낮은 인지도, 부적절한 현장적용 등으로 인한 교통사고의 위험 및 정체가중, 교차로 꼬리물기 위반행위 발생 등의 문제점들이 도출되었다. 현재 선진화 방안은 주로 직진우선신호, 비보호좌회전, 점멸신호 운영 등 교통신호운영 방법 개선을 위주로 추진되고 있는데, 향후 최종적으로 교통운영체계 선진화 모든 과제가 복합적으로 도입될 경우 현재 보다는 더 나은 효과를 기대할 수 있을 것이다.

• 가정과삶의질연구
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• 제27권2호
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• pp.173-189
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• 2009

The purpose of this study was to find and provide the prevention method by examining the Effects of Maternal Negative Behaviors and Children's Attachment Stability on Children's Aggression. The subjects were 339 children of fifth or sixth graders in Seoul. The questionnaires were derived and modified from Young Ae Park (1995) for the maternal negative behavior, Children's attachment stability from Ok Jung (1998) and Children's aggression from In Sun Jung(2006). The date were analyzed with descriptive statistics, t-test, F-test, Pearson's correlations and hierarchical multiple regression by SPSS 14.0 program. The major findings were as follows : First, the result showed there was average level of negative relationship when mother's negative behavior was compared with children's attachment whereas it showed average level of positive relationship in aggression. And also, low level of negative relationship showed between the attachment and aggression. Second, studying the influence it gives to the children's reaction and aggression after adding the children, parent and the family's popular social variables into the maternal's negative behavior, the outcomes showed the maternal's discipline and defiance upbringing had influenced the highest and tolerance, negligence was second high. This study mainly looked at the relationship between the maternal negative behavior with the children's attachment and aggression, but not like the nations pre.studied results, changes in human relation were examined with identifying the main several factors that bring greatest influence to the children's attachment and aggression. In conclusion, the children's attachment and aggression's the cause of the children's attachment and aggression were mainly from the maternal discipline and defiance upbringing. Based on this conclusion, it is essential for the maternal to have a sound discipline with right minds for endeavor to bring up the children with bring minds with less aggression and composed attachment.

• 지역사회간호학회지
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• 제31권3호
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• pp.256-268
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• 2020

Purpose: This study aimed to explore ecological factors and strategies for childhood obesity prevention targeting vulnerable children using a community-based participatory research (CBPR) methodology. Methods: The CBPR was conducted by following basic process steps. Participants were 12 community stakeholders such as community child center directors (n=4), vulnerable children's mothers (n=3), community health center officials (n=2), and lay health advisors (n=4); they were purposively sampled from K municipal county in Seoul, South Korea. The qualitative content analysis was performed to explore main themes of the ecological factors and strategies by using data obtained from 5 times of focus group interview. Results: Twelve ecological factors associated with childhood obesity prevention were identified: Intrapersonal factors including emotional overeating; interpersonal factors including permissive parenting style of children's eating behaviors; organizational factors including social workers' less educational opportunities; and community/policy factors including less government financial support. Four ecological strategies for childhood obesity prevention were addressed: Developing obesity prevention programs targeting vulnerable children' lifestyles; promoting parents' active participation in education; building healthy meal service environments through empowering social workers; and building supportive community environment and securing community resources for child obesity prevention. Conclusion: Our findings may be informative in terms of providing a comprehensive understanding of multi-level ecological barriers against vulnerable children' obesity prevention and, moreover, guiding multi-level strategies for preventing childhood obesity targeting children enrolled in community child centers.