• KOREAN JOURNAL OF CROP SCIENCE
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• v.63 no.1
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• pp.48-56
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• 2018

The present study aimed to understand the variation in seed germination according to the storage period after harvest in the accessions of cultivated and weedy types of Perilla species in Korea. In this milieu, we investigated the germination rate and germination energy of 59 Perilla accessions (15 cultivated var. frutescens type I, 4 cultivated var. frutescens type II, 20 weedy var. frutescens, and 20 weedy var. crispa), which were harvested in autumn 2016. The accessions of cultivated var. frutescens (type I) showed an average germination rate of 80.8% 6 months after harvest; however, the accessions of weedy var. frutescens and var. crispa showed an average germination rate of 0.9% and 8.7%, respectively. Furthermore, the accessions of cultivated var. frutescens (type II) showed an average germination rate of 18.2%. The accessions of cultivated var. frutescens (type I) showed an average germination energy of 75.8%, while the accessions of weedy var. frutescens and var. crispa showed an average germination energy of 0.6% and 6.9%, respectively. In addition, the accessions of cultivated var. frutescens (type II) showed an average germination energy of 14.3%. The germination rate and germination energy for the accessions of cultivated and weedy types of Perilla species increased marginally at 2 months from the first month after harvest. However, it did not significantly increase until six months after that. According to our results, there are two types of cultivated var. frutescens, namely, type I, which showed high germination rate and germination energy, and type II, which showed low germination rate and germination energy. The results of this study will provide basic information to understand variations in the germination of seeds during 6 months of storage period after harvest in the accessions of cultivated and weedy types of Perilla species in Korea.

• The Korean Journal of Pesticide Science
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• v.16 no.4
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• pp.336-342
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• 2012

This study was carried out in order to compare the residue levels of pesticides among lettuce, kale and perilla leaf depending on the morphological characteristics of plant. Residue levels were investigated at the zero, second, fifth days after last application, 9 species of systemic or non-systemic pesticide were twice applied with 7 days interval by knapsack power sprayer with 2 heads fan shape nozzle. Ratios of leaf area to weight ($cm^2$ $g^{-1}$) were 58 of perilla leaf, 27 of lettuce and 23 of kale. Ratios of leaf area to weight of perilla leaf was 2.1 times higher than that of lettuce. Residue levels of perilla leaf were 1.3 to 2.3 times higher than those of lettuce at the day of spraying and 1.3 to 3.3 times higher at the fifth day. Therefore the differences of pesticide residues between perilla leaf and lettuce were affected by the ratio of leaf area to weight. Residue levels in lettuce were 2.4 to 7.3 times higher than those in kale at the day of spraying because the adhesive effect of pesticide particles on kale leaf was low.

• Journal of the Korean Society of Food Science and Nutrition
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• v.31 no.2
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• pp.277-283
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• 2002

In the present study, adverse effects of megadose of dietary perilla oil were investigated in an experimental model consisted of 6 groups of Wistar rats. To compare the adverse effects of megadose perilla oil with different kind of dietary fat, rats were fed one of the following diets for one month: 10% beef tallow (B$_1$B), 10% corn oil (C$_1$B), 10% perilla oil (P$_1$B), 20% beef tallow (B$_2$B), 20% corn oil (C$_2$B), and 20% perilla oil (P$_2$B) diet. The body weight gain rate seemed to be more affected by the size of fat contents than the species of fat in the diet, so the body weight gain rate of 20% fat groups were significantly higher than those of 10% fat groups in spite of the larger amount of flood intake in 10% fat groups than in 20% fat groups. The levers of plasma triglyceride and total-cholesterol in 20% fat groups were significantly increased in dose dependent fashion when compared to 10% groups, the values of beef tallow (B$_2$B) group being the highest among all groups. Plasma glutainic pyruvic transferase activities and level of blood urea nitrogen had a tendency to increase along with increase of fat contents (%) in diets, the values of P$_2$B group, the highest among all groups, being beyond the normal levers. The plasma carbon dioxide concentration of P$_2$B group was the highest in all groups and exceeded the normal value, there being no significant difference among the plasma carbon dioxide concentration of others groups. The results showed that large dose and long term intake of dietary perilla oil had some adverse effects on hepatic and other organic functions in rats.

• Korean Journal of Pharmacognosy
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• v.38 no.2 s.149
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• pp.170-175
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• 2007

Activated microglia by neuronal injury or inflammatory stimulation overproduce nitric oxide (NO) by inducible nitric oxide synthase (iNOS) and reactive oxygen species (ROS) such as superoxide anion, resulting in neurodegenerative diseases. The toxic peroxynitrite (ONOO$^-$), the reaction product of NO and superoxide anion further contributes to oxidative neurotoxicity. We tried to evaluate the effects of two kinds of varieties of Perilla frutescens var japnica Hara on the NO production in lipopolysaccharide (LPS)-activated microglia. The perilla cultivars of Namcheondeulkkae (NC) and Boradeulkkae (BR) were developed by pure line from the local variety and by a cross between 'deulkkae' and 'chajogi', respectively. Spirit, hexane, chloroform and butanol fractions of the leaves of NC and BR inhibited the production of NO in LPS-activated microglia. The fractions of BR showed stronger activity than NC and the spirit extracts was the most potent in both cultivars. The solvent fractions of BR suppressed the expression of protein and mRNA of iNOS in LPS-activated microglial cells. Moreover, the extracts of NC and BR showed the activity of peroxynitrite scavenging in cell free bioassay system. These results imply that Namcheondeulkkae and Boradeulkkae might have neuroprotective activity through the inhibition of NO production by activated microglial cells and peroxynitrite scavenging activity.

• International Journal of Industrial Entomology and Biomaterials
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• v.21 no.1
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• pp.133-137
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• 2010

The perilla leaf pyralid moth, Pyrausta panopealis Walker (Lepidoptera: Pyralidae), is a serious pest damaging to leaf perilla. In order to establish the life parameters of P. panopealis for eventual purpose of control, the developmental span of each stage was investigated under five temperature regimes ($20^{\circ}C{\sim}30^{\circ}C$). The larval period of P. panopealis was longest as 26.8 days at $20^{\circ}C$ and shortened as temperature goes up to $30^{\circ}C$ as 11.3 days. Survivorship of the larval P. panopealis was the highest at $27.5^{\circ}C$ as 82.5%, whereas that of other temperatures ranged from 40% ($20^{\circ}C$) to 60.0% ($30^{\circ}C$), indicating that the P. panopealis appears to favor somewhat higher temperature. In addition to larval period, the duration of egg, prepupal, and pupal period also were shortened sharply as temperature goes up, whereas the duration of adult stage shortened a maximum of only two days as temperature goes up. After the perilla leaf pyralid moths were successfully stabilized in indoor environment the larvicidal efficacy of ten on-the-market environmentfriendly agricultural materials (EFAMs) that were previously selected from the result of other moth species was tested. Seven of the ten tested showed more than 90% of mortality within 12 hrs and reached nearly up to 100% within 24 hrs, but the remaining three showed less than ~70%.

• Research in Plant Disease
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• v.12 no.3
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• pp.158-163
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• 2006

Sclerotinia rot caused by Sclerotinia sclerotiorum frequently causes serious and unpredicable yield losses of the leaves of perilla growing under structured cultivation as vegetable in Korea. Temperature for mycelial growth ranged from 5 to $30^{\circ}C$ with optimum temperature d $20^{\circ}C$. Sclerotia were formed fewer at low temperature, but their dry weight was heavier than that at high temperature. The apothecia were formed from the sclerotia that buried up to 3 cm soil depth at $15^{\circ}C$ in moisture condition. The incidence of perilla Sclerotinia rot caused by S. sclerotiorum was observed throughout the growing season at greenhouse. The occurrence of this disease was especially severe from January to February of low temperature period. The average incidence rates of this disease was up to 15%. The significant occurrence of this disease was showed mainly in the continuous cropping field for more than five years. The incidence of this disease increased according to the increase of continuous cropping year. The incidence rates of this disease reached up to 20% in the continuous cropping field for ten years. Also it was firstly investigated the natural infection caused by S. sclerotiorum on weed plants, Gnaphalium affine and Mazus pumilus in farmer's field. The casual fungus showed pathogenicity on 11 weed plants species tested, and more severe pathogenicity on G. affine, Latuca indica and Ixeris dentata included in the family Compositae. This result suggests that effective crop rotation and weed eradication can be the method for organic control of perilla sclerotinia rot, and sudden outbreaks of this disease on perilla growing under structure after paddy rice call be explained by the presence of weed hosts.

• Journal of Life Science
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• v.31 no.5
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• pp.511-519
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• 2021

The Perilla frutescens var. japonica HARA is widely cultivated in Korea for vegetable leaves and oil seeds. Perilla species have been used for food and medicine and are known to contain many functional compounds. In this study, we investigated the functional compound contents of Perilla during its growth stages to analyze the optimal harvest time and conditions. The contents of the Perilla sprouts were analyzed according to culture environment and days of growth. Sprouts grown in soil under natural light conditions showed high rosmarinic acid (23.19±0.16 mg/g) and GABA (0.55±0.05 mg/g) content. Therefore, the results suggest that 6 to 8 days after sowing in soil under natural light conditions was the optimum harvest condition for sprouts. Also, the functional compounds of Perilla were analyzed according to growth stage and plant part. As a result, caffeic acid and rosmarinic acid exhibited the highest content in the stage from vegetative growth to reproductive growth (0.28±0.03 ~ 0.30±0.07 mg/g rosmarinic acid and 20.60±7.02 ~ 19.37±3.18 mg/g caffeic acid), and luteolin and GABA showed the highest content in the reproductive growth stage and in the early stages of vegetative growth, respectively (31.11±2.98 ~ 22.35±1.64 ㎍/g luteolin and 0.42±0.09 ~ 0.37±0.04 mg/g GABA). It was confirmed that the content of caffeic acid (0.34±0.03 mg/g), rosmarinic acid (55.22±9.33 mg/g) and luteolin (1,044.89±6.72 ㎍/g) was the highest during the bolting stage. Overall, we identified the timing of the highest level of functional compounds in the sprouts and mature leaves of Perilla. These results suggest a suitable harvest time and conditions for sprouts and leaves for the use of Perilla as a functional material.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2002.09a
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• pp.195-199
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• 2002

This study is for know chemistry of the representative soils from Keumsan, its relationships with dissolved compositions, and its absorption relationships within the flora. It has been done for two comparisons: 1) phyllite, shale and granite(PSG), and 2) granite and coal bearing shale(GC). Among the soil leachate of the PSG, cations are mainly low in the phyllite area while anions are mainly low in the granite area. In the soil compositions, Ca and Mg of the beans are high and Na is high in the granite area while Ca and Na of the perilla are high in the granite area and Mg is high in the phyllite area. In th both species, Na for the granite area and Mg for the phyllite area are high while Ca for the shale area is low. Among the flora, shale area shows low Ca and Mg contents while granite area shows slightly high Na contents, regardless the species. Compared with beans, perilla is low in the Mg and high in the Na contents. These relationships show that the contents of the soil leachate do not reflect absorption within the flora. Among the soil leachate of the GC, shale area is high in the most of the elements. Especially, SO$_4$ is over 15 times high In the phyllite area. In the soil, granite area shows high Ca, Na and low Mg contents. In the flora, the Miscanthus sinensis shows high Ca and Mg contents in the granite area while the Artemisia vulgaris shows high Ca, Ca and Mg contents in the shale area. These relationships for contents of the flora and soil leachate suggest that the flora has a different absorption according different species.

• Journal of the Korean Society of Food Science and Nutrition
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• v.5 no.1
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• pp.69-74
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• 1976

Six kinds native perilla selected with superior species were cultivated by the same condition. General compositions, inorganic substances and free amino acid of a species of seeds and leaves were analyzed. The results obtained are summarized as follows ; 1. Fat components of seeds obtained from Incheon and Kunsan were 52 and 50.87% respecand these of other species were over 45%. 2. Protein components of seeds obtained from Daegu and Daejeon were 22.31 and 19.15% respectively, and these of leaves were 3 to 5%. 3. Vitamin C and $B_2$ contained in leaves were above 60mg and $170\;{\mu}g%$. 4. Leaves of perilla contained 2 to 10 times of inorganic substance comparing with seeds. Potassium was much in leaves and seeds and calcium showed plently in leaves. 5. Free amino acid in seeds of Daejeon contained 54.2mg% of arginine, 23.9mg% of alanine, and that of leaves contained 24.8mg% of glutamic acid and 9.1mg% of alanine.

• Korean Journal of Environmental Agriculture
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• v.17 no.2
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• pp.122-126
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• 1998

This experiment was conducted to verify the responses of major crop species to saline stress.To determine the saline tolerance of crop species, sevral crop species were cultivated under sand-culture system using Hoagland's nutrient solution whit 200mM NaCl. The crop species showing saline tolerance were cotton(Gossypium indicum), maize(Zea mays), barely(Hordeum vulgare), and wheat(Triticum aestivum) but perilla (Perilla frutescens) and leguminous crops, mung bean(Phaseolus radiatus), azuki bean(Phaseolus angularis) and soy bean(Glycin max) showed very por tolerance. One the typical symptom was the darkening of leaf color due to increase of chlorophyll concentration.Among of the plant families, Fabaceae was the most susceptible but crop species belonging to Poaceae were more proper for cultivating on reclaimed tidal land in the course of desalinazation. It was suggrsted that the crop species belonging to Fabaceae, a sensitive family to soil salinity, must be cultivated when the soil salinity decreased below 10ds/m. To know the critical salinity level for crop growth,salinity of saline soil collected from reclaimed tidal land was adjusted from 10.0ds/m tp41.7ds/m with tap water. It was suggested that the ECs of the soil in which the plant height of each crop spicies was reduced to 50% of control plant were 22.6 and 21.7 ds/m in rice, barley, corn, mung bean, and soy bean,respectively.