• Korean Journal of Microbiology
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• v.30 no.3
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• pp.154-159
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• 1992

Forniation ancl ~regcncration oi' conitlial pro1oplast of Pc, ti~i.rlli~in~~~ c~rr~~culo.hryupmel.- - czllulolytic Ihngus. were examined. By using Novozyme 134(1'!/0 w/c) as a cell wall lytic enzyme. the highest yield of protopl;~sts exceeding 501%, war obtained from the qwollen conidiosporcs preincubatrd in the minimal medium containing 2-tleoxy-D-glucose(2-UC;. 75 pglml) for 10-11 11. No protoplast were obtained horn dormant spores. The regeneration frequency of the protoplasts was 49.2'!11. which was higher than that of mycclium originated protoplast (4.6-17.X1X, . in 0.6 M MgS04. pH 5.6). 'l'lie best osmotic stahilizcr ror the isoaltion and regcueration of thc protoplast was 0.6 M iimmonium sulfatc and 0.6 M magnesiuni sulfhte. respectively. 'I'lie process of the protoplast isolaiton l'ro~n swollen cnnirliospore ancl regeneration ha\, ing two pattcrns from protoplast were obsen'etl through light microscope.