• YAKHAK HOEJI
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• v.57 no.2
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• pp.125-131
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• 2013

Biologically active peptides, including growth factors and cytokines, participate in various biological processes in human skin. They could provide a great advantage of maintaining healthy skin. Many peptide growth factors like epidermal growth factor (EGF) and human growth hormone (hGH) have been used in cosmetic formulations. The delivery of peptide growth factors across the Stratum corneum, however, seems not sufficient because of their physical properties such as high molecular weight and hydrophilicity. So increasing the penetration of growth factors of interest into skin would be a major concern for ensuring their maximum biological efficacy. In this study, we have identified several skin penetration-enhancing peptides which facilitate delivery of growth factors, when fused at N-terminus of the target protein, into skin. For efficient and rapid screening, we constructed a skin-penetrating assay system using Franz cell and porcine skin. Next, we carried out phage display screening using M-13 bacteriophage with random 12 -amino acid library on its coat protein P3 on that system. After several selection rounds, peptide sequences facilitate the penetration of phages through the porcine skin were identified from a large population of phages. We found that phages with the most potent peptide (S3-2, NGSLNTHLAPIL) could penetrate the porcine skin eight times more than those with control peptide (12 mino acids scrambled peptide). Furthermore, growth factors conjugated with S3-2 peptide penetrate porcine skin three to five times efficiently than non-conjugated growth factors. In conclusion, our data shows that the skin penetration-enhancing peptide we have characterized could increase the delivery of growth factors and is useful for cosmeceutical application.

• Proceedings of the KWS Conference
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• 1997.05a
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• pp.148-150
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• 1997

• Tunnel and Underground Space
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• v.13 no.6
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• pp.444-454
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• 2003

Rock fragmentation technique by cutter penetration has widely been used in the mechanical tunnel excavation. Microcracks propagate and interact because of locally concentrated high stress induced by cutter penetration. which is caused by heterogeneity of rocks. In this study Weibull distribution function and degradation index are used to consider the strength heterogeneity of a rock and the degradation of rock properties after failure. Through the numerical analyses, it is shown that the lateral pressure has an important influence on the rock fragmentation. In the single cutter penetration, large chips are formed as lateral pressure increase. The cutter spacing is also an important factor that affects the rock fragmentation in the double cutter penetration. The fragmentation efficiency of the double cutter penetration is better when cutter spacing is 70 mm than 40 mm and 100 mm. From the results, it is expected that this study can be applied to a TBM tunnel design by understanding of chipping process and mechanism of rock due to cutter penetration.

• Journal of Welding and Joining
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• v.15 no.3
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• pp.79-87
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• 1997

This study was performed to evaluate basic characteristics of electron beam welding process for a 9% Ni steel plate. The principal welding process parameters, such as working distance, accelerating voltage, beam current and welding speed were investigated. The AB (Arata Beam) test method was also applied to characterize beam size and energy density of the electron beam welding process. The electron beam size was found to decrease with the increase of accelerating voltage and the decrease of working distance. So, in case of high voltage (150kV), spot size and energy density of electron beam were revealed to be 0.9mm and $6.5\times10^5W/\textrm{cm}^2$ respectively. The accelerating voltage among the welding parameters was found to be the most important factor governing the penetration depth. When the accelerating voltage of electron beam was low ($\leq$90kV), beam current and welding speed did not affect on the penetration depth significantly. However, in case of high voltage ($\geq$120kV), the depth of penetration increased very sensitively with the increase of beam current and the decrease of welding speed.

• Korean Journal of Agricultural Science
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• v.24 no.2
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• pp.226-236
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• 1997

The results obtained by elasto-plastic analysis method about the displacement, deformation and stability on the soft ground excavation using sheet pile were summarized as follows ; 1. In the case of strut 1 step, the maximum wall displacement value in the first and the second excavation was small, but it increase remarkably after the third excavation and when the excavation depth was 8m, the point of maximum wall displacement was shown 0.75H~0.8H. 2. The value of safety factor(Fs) was increased with increasing of the penetration depth of sheet pile, cohesion and internal friction angle of ground. Safety factor was mostly effected by penetration depth of sheet pile and more effected by cohesion than internal friction angle of ground. 3. Since the deformation of sheet pile of this ground from the results of analysis and measurement increased remarkabaly after 6m excavation depth, it was desirable that the point of strut installation was GL-6m. 4. Safe excavation depth on ground by analysis considered penetration depth, cohesion and internal friction was shown at the table 3.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.45 no.2
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• pp.175-184
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• 2019

The epidermal growth factor (EGF) has a intrinsic function of inducing growth and proliferation of cells through interacting with cell membrane receptors in human epidermis and dermis layer. These functions of EGF are used as a main ingredient for wound healing medicines and anti-aging cosmetics. As a cosmetic ingredient, the EGF has a problem in exhibiting its natural efficacy due to the lack of the ability to penetrate through the stratum corneum, which is known as the skin barrier. In this study, a recombinant human epidermal growth factor ($MTD_{151}-EGF$) fused with the macromolecule transduction domain $(MTD)_{151}$ with the skin penetration ability was developed to improve the skin penetration efficiency of the EGF. Expression of $MTD_{151}-EGF$ was performed in E. coli transformed with a vector encoding the $MTD_{151}-EGF$ gene and then purified. The purified $MTD_{151}-EGF$ was evaluated using cell proliferation assay, cytotoxicity test and skin penetration test by franz diffusion cell assay and artificial skin. Cell proliferation activity of $MTD_{151}-EGF$ purified to high purity of 99% or above was equivalent to the EGF or better, and cytotoxicity was not observed. In addition, the $MTD_{151}-EGF$ showed an excellent penetration efficiency compared to the EGF in the skin penetration test with EGF and $MTD_{151}-EGF$ labeled by FITC in an artificial skin penetration model. Based on the quantitative analysis of the penetrating substance using franz diffusion cell assay, the amount of penetration was about 16 times more than that of EGF. These results can be regarded as an effective alternative to improve the existing physical transdermal penetration method related to the use of various active ingredients for cosmetics.

• 전기의세계
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• v.29 no.9
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• pp.594-598
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• 1980

This paper describes an analytical method on the starting torque of hysteresis motor, taking account of penetration effects of magnetic-field and eddy-current into the rotor, to the elliptical approximation method of hysteresis-loop. By the above method, it have obtained the torque of rotor ring with non-magnetic and non-conductive material arbor, and the results are concerned and compared with that of computed by aid of callibration factor, k=1+exp(-2t$_{r}$/.delta.).)

• Journal of the Korean Geotechnical Society
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• v.24 no.4
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• pp.89-100
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• 2008

Applying graph and correlation developed in other countries to the domestic field has limits. Therefore, it is necessary that Piezocone factor should be determined by including ground characteristics obtained from various test results in the domestic site for better application. In this study, laboratory tests and field tests were performed to find the characteristics of soft ground which was widely distributed around Siwha lake. A comparative analysis of these tests with Piezocone penetration test was performed and the Piezocone factor was estimated by correlationship analysis of undrained shear strength and cone resistance. Also, an appropriate Piezocone factor in the study site was reestimated by utilizing statistical analysis method for deriving reliable result.

• Journal of the Korean GEO-environmental Society
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• v.15 no.1
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• pp.35-41
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• 2014

Many infra suructure such as road, railway, building and utility foundations have been damaged by the repeated freezing and thawing of the soil during winter and spring every year in seasonal frost region. The frost penetration depth is most important factor in the design of structure such as road, railway and building in seasonal frost region. This paper presents the results of calculation of frost penetration depth and frost heaving in concrete track for railway construction. Model concrete track were installed near the railway track in Gangwon, Gyeonggi, Choongbuk province and frost penetration depth were measured using methylene blue frost penetration depth gauge. Model concrete track in Cheolwon, frost heaving of concrete track were also evaluated. The measure of maximum frost penetration depth and frost heaving can be applied to design railway track for cold region in Korea.

• Journal of the Korean Society for Marine Environment & Energy
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• v.6 no.3
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• pp.37-44
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• 2003

To know the penetration behavior of spilled oil into sandy beach sediment is very important, because the penetration depth of the stranded oil into the sediments is one of the most significant information to know effect of spilled oil on biological communities and to set up cleaning method. The purpose of this study is to clarify the effects of wave and/or tidal action on penetration of spilled oil into the sediments and to clarify main factor in oil penetration using sandy beach model. Specific conclusions derived from this study are as follows. Spilled fuel oil C penetrated into the sediments only by falling tidal fluctuation and not by wave action on sandy beach environment, and the first tide is most important for the penetration of stranded oil. Over 80% of bulk fraction in penetrated fuel oil C was concentrated to the top 2 cm sediment-layer. Moreover, the penetration of stranded oil into the sandy beach sediments was strongly correlated with the oil viscosity affected by temperature.