• 한국자원식물학회지
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• 제29권3호
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• pp.322-330
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• 2016

Understanding the genetic variation among landrace collections is important for crop improvement and utilization of valuable genetic resources. The present study was carried out to analyse the genetic diversity and associated population structure of 621 foxtail millet accessions of Korean landraces using 22 EST-SSR markers. A total of 121 alleles were detected from all accessions with an average of 5.5 alleles per microsatellite locus. The average values of gene diversity, polymorphism information content, and expected heterozygosity were 0.518, 0.594, and 0.034, respectively. Following the unweighted neighbor-joining method with arithmetic mean based clustering using binary data of polymorphic markers, the genotypes were grouped into 3 clusters, and population structure analysis also separated into 3 populations. Principal coordinate analysis (PCoA) explained a variation of 13.88% and 10.99% by first and second coordinates, respectively. However, in PCoA analysis, clear population-level clusters could not be found. This pattern of distribution might be the result of gene flow via germplasm exchanges in nearby regions. The results indicate that these Korean landraces of foxtail millet exhibit a moderate level of diversity. This study demonstrated that molecular marker strategies could contribute to a better understanding of the genetic structure in foxtail millet germplasm, and provides potentially useful information for developing conservation and breeding strategies.

• 한국조경학회지
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• 제33권1호
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• pp.71-80
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• 2005

In order to search for ecologically indicative characteristics on the street tree plots in Daegu area, plant communities and their floras were investigated. A total of 105 plots were collected and numerically analyzed by PCoA(Principal Coordinates Analysis). These plots were classified into 4 types containing 139 species, 97 genera, 42 families(including 37 exotic species): urban-dry type, urban-wet type, rural-dry type, rural-wet type. Habitat connectivity to the surrounding vegetation cover, extent and frequency of human impacts, and soil moisture recognizably were the main factors to allow the plots differentiation. Indicative species composition to these four types was generated as Eleusine indica-Eragrostis multicaulis-Oxalis corniculata to the urban-wet, Digitaria ciliaris-Eleusine indica-Eragrostis multicaulis to the urban-dry, Setaria viri-dis-Artemisia-Lactuca indica var. laciniata to the rural-wet, and Setaria viridis-Digitaria ciliaris-Erigeron canadensis to the rural-dry, respectively. Mean species number per plot for rural type was 2.5 times higher than for urban types. Street tree species representative to the rural-wet type is Zelkova serrata, which is a key species of potential natural vegetation in the alluvial land of Daegu area. Street tree plots were characterized by Eleusine indica showing the highest r-NCD value and also C4-assimilation grass plants. Views on the efficacy of the rural-wet type and the reinforcement of vegetation connectivity and soil moisture in rehabilitating street tree plots, are discussed.

• 대한한의진단학회지
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• 제20권2호
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• pp.67-75
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• 2016

Objectives There was a correlation between respiratory index and Profile of Mood States (POMS). However, no study has examined the relationship between hyperventilation and POMS. Therefore, this study showed differences in POMS subscales and respiratory patterns between hyperventilation group and normal group. Methods 20 healthy men and women were to complete Nijmegen and Korean-Profile of Mood States(K-POMS) questionnaire aimed at subjects (13 men, 12 women). By attaching a capnometer to the nasal cavity portion, end-tidal $PCO_2$ was measured. Also, marker was attached at Zhangmen, Juque, Shuifen. The movement of the marker was taken as a web cam. Statistical analysis Mann Whitney U test was used for the nonparametric methods. Results In the subscale of K-POMS were significant differences(Tension-0.001, Anger-0.007, Fatigue-0.002, Depression -0.004) between the normal group and the group with the hyperventilation. In addition, between the two groups were obtained a significant result(0.046) in the movement of the Shuifen acupoint. Conclusions Nijmegen questionnaire score is higher, the higher negative subscale scores of K-POMS. Also, differences in Nijmegen questionnaire score may help to determine the presence or absence of the abdominal breathing.

• Plant Breeding and Biotechnology
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• 제5권3호
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• pp.183-191
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• 2017

Finger millet (Eleusine coracana L. Gaertn.) is an important cereal crop in eastern Africa and southern India with excellent grain storage capacity and the unique ability to thrive in extreme environmental conditions. In this study, we analyzed the genetic diversity and population structure of finger millet using 12 developed microsatellites. By sequencing 815 clones from an SSR-enriched genomic DNA library, we obtained 12 polymorphic SSR markers, which also revealed successful amplicons in finger millet accessions. Using the developed SSR markers, we estimated genetic diversity and population structure among 76 finger millet accessions in Asia, Africa, and unknown origins. The number of alleles ranged from 2 to 9, with an average of 3.3 alleles. The mean values of observed heterozygosity and expected heterozygosity were 0.27 and 0.35, respectively. The average polymorphism information content was 0.301 in all 76 finger millet accessions. AMOVA analysis showed that the percentage of molecular variance among the populations was 1%, that among individuals was 5%, and that within individuals was 94%. In STRUCTURE analysis, the 76 finger millet accessions were divided into two subpopulations which had an admixture of alleles. There was a correspondence among PCoA, AMOVA, and population structure. This study may form the basis for a finger millet breeding and improvement program.

• Animal Bioscience
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• 제35권8호
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• pp.1121-1128
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• 2022

Objective: This study aimed to identify the genetic diversity and population structure of Mongolian horse populations according to the province of residence (Khentii, KTP; Uvs, USP; Omnogovi and Dundgovi, GOP; Khovsgol, KGP) using 14 microsatellite (MS) markers. Methods: A total of 269 whole blood samples were obtained from the four populations (KTP, USP, GOP, KGP) geographically distinct provinces. Multiplex polymerase chain reaction (PCR) was conducted using 14 MS markers (AHT4, ASB2, ASB17, ASB23, CA425, HMS1, HMS2, HMS3, HMS6, HMS7, HTG4, HTG6, HTG7, and VHL20), as recommended by the International Society for Animal Genetics. Capillary electrophoresis was conducted using the amplified PCR products, alleles were determined. Alleles were used for statistical analysis of genetic variability, Nei's DA genetic distance, principal coordinate analysis (PCoA), factorial corresponding analysis (FCA), and population structure. Results: On average, the number of alleles, expected heterozygosity (H_Exp), observed heterozygosity (H_Obs), and polymorphic information content among all populations were 11.43, 0.772, 0.757, and 0.737, respectively. In the PCoA and FCA, GOP, and KGP were genetically distinct from other populations, and the KTP and USP showed a close relationship. The two clusters identified using Nei's DA genetic distance analysis and population structure highlighted the presence of structurally clear genetic separation. Conclusion: Overall, the results of this study suggest that genetic diversity between KTP and USP was low, and that between GOP and KGP was high. It is thought that these results will help in the effective preservation and improvement of Mongolian horses through genetic diversity analysis and phylogenetic relationships.

• Journal of the Korean Data and Information Science Society
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• 제28권5호
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• pp.1043-1053
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• 2017

본 연구는 산양 7 품종을 대상으로 (Saanen (88), Laoshan (67), Toggenburg (32), Alpine (12), Anglonubian (9), Jamnapari (7), Black Bengal (4)) 13종의 초위성체 마커 (microsatellite marker)를 활용하여 유전적 다형성 분석을 실시하였다. 대립유전자 수는 4개 (INRA005) 부터 18개 (SRCRSP23)까지 확인되었으며, 관측이형접합율 ($H_{obs}$)과 기대이형접합율 ($H_{\exp}$) 그리고 다형성 정보지수 (PIC) 값은 각각 0.482 ~ 0.786, 0.476 ~ 0.923 그리고 0.392 ~ 0.915로 나타났다. 품종별 유전적 거리를 확인하기 위하여 실시한 주성분분석 (PCoA) 결과는 요인대응분석 (FCA) 분석과 유사한 결과를 보였으며, 동일개체출현빈도는 $2.47{\times}10^{-15}$으로 확인되었다. 따라서 본 연구 결과는 산양 품종 개량 및 보존에 있어 기초자료로써 유용한 자료로 활용 가능 할 것으로 사료된다.

• Annals of Clinical Neurophysiology
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• 제4권1호
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• pp.51-55
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• 2002

Background : An increased frequency of reproductive endocrine disorders and polycystic ovary(PCO) has been reported in women with epilepsy. A possible role of the seizure disorder or, of the use of antiepileptic drugs(AEDs) has been suggested as the pathogenic mechanism. The objective of the present study was to assess the prevalence of reproductive endocrine disorders, such as PCO or menstrual abnormalities, in a series of women with epilepsy, examining the possible relationships of these disturbances with different epilepsy syndromes and AED treatment. Methods : Thirty epileptic women, all of reproductive age and none pubertal, pregnant, or lactating, were evaluated by clinical endocrinological assessment, and pelvic ultrasonography. Seven patients were on valproic acid(VPA), nineteen on carbamazepine(CBZ), and four on diphenylhydantoin(DPH) treatment, respectively. Results : Menstrual irregularity was observed in 8 women(26.7%), dysmenorrhea in 7(23.3%), and premenstrual syndrome in 1(3.3%). Ultrasonographic examination revealed that one women(3.3%) showed polycystic ovary, 4(13.3%) had ovarian cyst(s), and 2(6.7%) had uterine myoma, respectively. There was no difference in the prevalence of menstrual abnormalities or polycystic ovary according to the different preparations of AEDs. Conclusions : Data from this investigation suggest that, in Korean reproductive age women, the treatment of AEDs and the kind of medication may not have a significant effect on the prevalence of menstrual abnormalities or ultrasonographic polycystic ovary.

• The Korean Journal of Ecology
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• 제28권2호
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• pp.85-92
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• 2005

경남 양산시 신불산의 습원(면적 14,000 $m^2$)에 대한 식물군락의 유형화와 동태에 대한 연구가 수행되었다. 습원 식물상은 감시대상이 되는 식물종 26종을 포함한42과 80속 105종이 기재되었다. Z.-M.학파의 연구방법으로 8개 식생유형이 분류되었다: 올챙이자리-바늘골군집(신칭), 이삭귀개-흰개수염군락, 진퍼리새-좀네모골군락(전형하위군락, 끈끈이주걱하위군락), 진퍼리새-하늘산제비난군집, 억새군락, 메역순나무군락, 떡갈나무-노린재나무군락, 신갈나무-노린재나무군락 주좌표분석(PCoA)은 습원 내 식물군락들이 초본층(지표면)의 직사광선 이용 경향성 및 건습환경 조건, 그리고 토양의 비옥성(중영양-빈영양)에 대응하여 식생 구조의 다변성과 분포 경향성을 보여주는 것으로 나타났다. 한반도 동남부 영남알프스 산악지역에서의 산지 중간습원을 대표하는 진퍼리새군강 발달의 주요 요인은 국지적인 지리지형 조건으로부터 보다 짧고 습한 생육기를 야기하는 보다 긴 운무기간에 의한 냉각효과와 같은 기후 조건이 강조되었다. 양산습원은 인간간섭에 의한 습원식생과 자연 습원식생으로부터 무제치습원과 비교되었다.

• Animal Bioscience
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• 제36권8호
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• pp.1221-1227
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• 2023

Objective: The present study aimed to investigate the effect of dietary lutein on egg production, follicles, reproductive hormones, fertility, hatchability, and oxidative injury indexes of hens. Methods: Treatments consisted of a control diet (CON) and three lutein-supplementing diets at 25 (L1), 50 (L2), or 75 (L3) mg/kg of diet. Egg production was measured using 576 Arbor Acres breeder hens at 61 to 65 wk and follicles grades, reproductive hormones, fertility, hatchability, tissue lutein contents, and oxidative injury indexes were determined at 65 wk. Results: The results showed that at 65 wk, lutein- supplementing diets increased (p<0.05) egg production, follicular grades, fertility, hatchability, estradiol (E2), luteinizing hormone, progesterone (PROG), lutein content in the serum and yolk, compared to CON. L2 and L3 showed more pronounced (p<0.05) effects on egg production, PROG, and yolk lutein content than L1. With the increase of lutein doses from 25 to 75 mg/kg, there were linear increases (p<0.05) in egg production, lutein content, and PROG, and a quadratic trend (p<0.05) in E2. For the oxidative injury products, lutein-supplementing diets decreased (p<0.05) malondialdehyde (MDA) and protein carbonyl (PCO) in the serum, MDA and 8-hydroxy 2 deoxyguanosine (8-OHdG) in the yolk. There were linear decreases (p<0.05) in 8-OHdG in the serum, MDA, PCO, and 8-OHdG in the yolk, a quadratic trend (p<0.05) on serum 8-OHdG. Conclusion: It is concluded that lutein supplementation can improve egg production and fertility by beneficially regulating reproductive hormones and oxidative status in aged hens.

• 대한물리치료과학회지
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• 제6권3호
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• pp.63-82
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• 1999

Recent studies have revealed that Nitric oxide(NO) was one of the demonstration for the physiological regulator, endothelial derived relaxing factor(EDRF) and that NO was produced by ultraviolet irradiation in human. Thus, the present author have carried out a experimental study on the change of hematological, histological value of ultraviolet irradiation in sprague-dawley rats. The subjects were divided into four groups of ten rats each selected at random. There were 4 groups: 1. no irradiation control; 2. ultraviolet $75mJ/cm^2$ irradiation group; 3. ultraviolet $150mJ/cm^2$ irradiation group; 4. ultraviolet $225mJ/cm^2$ group. After a irradiation, hematological and histological tests were performed to observe erythrocyte, hemoglobin, hematocrit, MCH, MCHC, MCV, $O_2$ saturation, pH, $PO_2,\;PCO_2$ value and to observe histological changes. In hematological tests, erythrocyte, hemoglobin, hematocrit significantly increased in $75mJ/cm^2$ than control group and more $150mJ/cm^2$ ultraviolet irradiation group respectively. Also In blood gas tests, $PO_2$ significantly increased in $75mJ/cm^2$ and more $150mJ/cm^2$ group than control group. Whereas $PCO_2$ significantly decreased in $75mJ/cm^2$ and more $150mJ/cm^2$ group than control group (Duncan-Tukey test, P<0.05). In histological tests, control and $75mJ/cm^2$ group unchanged, but more $150mJ/cm^2$ group changed that it was cytolysis, cytotoxic effect, acanthosis, proliferation of keratinocyte, appearance of amorphous cell and pyknotic nucleus, production of sunburn cell. In conclusion, the present author results support the importance of the relation between NO effect and hematological, histological value by ultraviolet B irradiation.