• BMB Reports
• /
• 제36권3호
• /
• pp.305-311
• /
• 2003

A modified actinomycin D was prepared with a hydroxyl group that replaced the amino group at the chromophore 2-position, a substitution known to strongly reduce affinity for double-stranded DNA. Interactions of the modified drug on single-stranded DNAs of the defined sequence were investigated. Competition assays showed that 2-hydroxyactinomycin D has low affinity for two oligonucleotides that have high affinities ($K_a\;=\;5-10{\times}10^6\;M^{-1}$ oligomer) for 7-aminoactinomycin D and actinomycin D. Primer extension inhibition assays performed on several single-stranded DNA templates totaling around 1000 nt in length detected a single high affinity site for 2-hydroxyactinomycin D, while many high affinity binding sites of unmodified actinomycin D were found on the same templates. The sequence selectivity of 2-hydroxyactinomycin D binding is unusually high and approximates the selectivity of restriction endonucleases. Binding appears to require a complex structure, including residues well removed from the polymerase pause site.

• 음성과학
• /
• 제7권2호
• /
• pp.209-224
• /
• 2000

This paper proposes an automatic detection method of intonational and accentual phrases in Korean standard continuous speech. We use the pause over 150 msec for detecting intonational phrases, and extract accentual phrases from the intonational phrases by analyzing syllables and pitch contours. The speech data for the experiment are composed of seven male voices and two female voices which read the texts of the fable 'the ant and the grasshopper' and a newspaper article 'manmulsang' in normal speed and in Korean standard variation. The results of the experiment shows that the detection rate of intonational phrases is 95% on the average and that of accentual phrases is 73%. This detection rate implies that we can segment the continuous speech into smaller units(i.e. prosodic phrases) by using the prosodic information and so the objects of speech recognition can narrow down to words or phrases in continuous speech.

• 대한전기학회:학술대회논문집
• /
• 대한전기학회 1999년도 하계학술대회 논문집 C
• /
• pp.1120-1122
• /
• 1999

This paper introduces a clustering for Central Control System in New Distribution Automation System. There are three primary benefits to use clustering: improved availability, easier manageability and more cost-effective scalability. Availability: Clustering can automatically detect the failure of an application or server and quickly restart it on a surviving server. Clients only experience a momentary pause in service. Manageability: Clustering lets administrators quickly inspect the status of all cluster resources and easily move workload around onto different servers within a cluster. Scalability: Applications can use the Clustering services through the MSCS Application Programming Interface(API) to do dynamic load balancing and scale across multiple servers within a cluster.

• 대한음성학회:학술대회논문집
• /
• 대한음성학회 2006년도 춘계 학술대회 발표논문집
• /
• pp.89-93
• /
• 2006

This paper presents ETRI broadcast news speech recognition system. There are two major issues on the broadcast news speech recognition: 1) real-time processing and 2) out-of-vocabulary handling. For real-time processing, we devised the dual decoder architecture. The input speech signal is segmented based on the long-pause between utterances, and each decoder processes the speech segment alternatively. One decoder can start to recognize the current speech segment without waiting for the other decoder to recognize the previous speech segment completely. Thus, the processing delay is not accumulated. For out-of-vocabulary handling, we updated both the vocabulary and the language model, based on the recent news articles on the internet. By updating the language model as well as the vocabulary, we can improve the performance up to 17.2% ERR.

• 대한전자공학회:학술대회논문집
• /
• 대한전자공학회 2001년도 하계종합학술대회 논문집(1)
• /
• pp.341-344
• /
• 2001

H.323 proposes to use Mobile IP and H.323 ad hoc conference signaling to provide Handoff function to an H.323 mobile terminal. But the H.323 ad hoc conference signaling has a drawback. It requires an H.323 terminal to do a complex conference signaling which takes a longer signaling time. In this paper, we propose an GK architecture that provides Handoff function effectively using 3$^{rd}$ P'||'||'||'||'||'&'||'||'||'||'||'R(Third party initiated Pause and Rerouting) signaling which are done through H.245 logical channel. The GK which is implementing 3$^{rd}$ P'||'||'||'||'||'&'||'||'||'||'||'R signaling only requires an H.323 endpoint to do the H.323 basic signaling in reestablishing media channel and gives the faster Handoff signaling. To do this, our GK has derived H.245 control channel using tunneling f3r all H.323 calls including the fast connect calls which enable terminals communicate each other if they doesn't have H.245 control channel .municate each other if they doesn't have H.245 control channel .

• 대한전자공학회:학술대회논문집
• /
• 대한전자공학회 2001년도 하계종합학술대회 논문집(1)
• /
• pp.225-228
• /
• 2001

We describe the implementation of MPEG-4 Systems for Video on Demand services. We discuss the issues in implementint MPEG-4 Systems, specially Object Description and Elementary stream management(Buffer control, Synchronization). The file-server pump the multiplexed streams to the client through the DMIF. Interaction between server and client is done with User Command(play, pause, stop, fast foward, ...). Stream data are multiplexed off-line. In advance, the file-server read multiplexed streams and pumps it to MPEG-4 terminal(client). Our system can be used in various application not only Video on Demand services, but also Internet broadcasting, remote surveillance, mobile communication, remote education, etc.

• 대한전기학회:학술대회논문집
• /
• 대한전기학회 1996년도 하계학술대회 논문집 C
• /
• pp.1854-1856
• /
• 1996

This study describes the minimum ignition limit for LPG-Ai-r mixtures by switching sparks in radio-frequency limits using RF power supply and IEC type ignition spark apparatus. As a result, the minimum ignition limit voltage is increased in proportional to the rate of increasing of frequency in LPG-Air mixed gas. Especially, increment between 10[kHz] and 30[kHz] is typical. It is considered that ignition is caused by one discharge until 10 [kHz] and, beyond 10[kHz] ignition is caused by more than two discharges. The reason is analysed that energy loss is caused by existing pause interval between discharges.

• 대한기계학회논문집B
• /
• 제28권12호
• /
• pp.1511-1520
• /
• 2004

A flow visualization of the two-dimensional rigid fling-clap motions of the flat-plate wing are performed to gain knowledge of butterfly mechanisms that might be employed by butterflies during flight. In this numerical visualization, the time-dependent Navier-Stokes equations are solved for cyclic fling and clap types of wing motion. The separation vortex pair that is developed in the fling phase of the cyclic fling and clap motion is observed to be stronger than those of the fling followed by clap and pause motion(1st cycle motion). This stronger separation vortex pair in the fling phase is attributable to the separation vortex pair of the outside space developed in the clap phase as it moves into the opening in the following fling phase. Accordingly, higher lift and power expenditure coefficients in the fling after clap phase is caused by the stronger separation vortex pair.

• 말소리와 음성과학
• /
• 제4권4호
• /
• pp.19-27
• /
• 2012

When two speakers read a text together, the produced speech has been shown to reduce a high degree of variability (e.g., pause duration and placement, and speech rate). This paper provides a quantitative analysis of speech rate variation exhibited in synchronous speech by examining the global and local patterns in two dialects of Mandarin Chinese (Taiwan and Shanghai). We analyzed the speech data in terms of mean speech rate and the reference of "Just Noticeable difference (JND)" within a subject and across subjects. Our findings show that speakers show lower and less variable speech rates when they read a text synchronously than when they read alone. This global pattern is observed consistently across speakers and dialects maintaining the unique local variation patterns of speech rate for each dialect. We conclude that paired speakers lower their speech rates and decrease the variability in order to ensure the synchrony of their speech.

• 대한음성학회:학술대회논문집
• /
• 대한음성학회 2000년도 7월 학술대회지
• /
• pp.283-300
• /
• 2000

This paper is intended to suggest how to improve Korean high school students' awareness of the pronunciation in order to foster communicative effectiveness. Initially it is focused on the tasks of listening to the suprasegmental aspects. The strategies used in the listening process are (1)discerning intonation units, (2)recognizing rhythm pattern, and (3)identifying contraction and linking in connected speech. The tasks including in each process are listening discrimination, guided practice activity, and listening and speaking activity. The teacher should avoid methods which yield discouraging outcomes and try to help students enjoy experience of success in doing exercises and activities. So I suggested: students put the slash on the pause perceptible to chunk the stream of speech into the intonation units, and mark the content words to internalize English rhythm. And then I suggested that students listen to pop song English in order to improve the awareness of function words and connected speech in the intonation unit.