• Journal of Pharmaceutical Investigation
• /
• v.41 no.1
• /
• pp.9-17
• /
• 2011

The objective of this work is to study transdermal delivery of calcitonin using iontophoresis and to evaluate various factors which affect the transdermal transport. We have studied the effect of polarity, current density, drug concentration, penetration enhancers (isopropyl myristate [IPM] and ethanol) and laser treatment on transdermal flux and the results were compared. We also investigated the iontophoretic flux from microemulsions containing calcitonin together with oleic acid (OA) or IPM. In vitro flux study was performed at $33^{\circ}C$, using side-by-side diffusion cell and full thickness hairless mouse skin. Anodal delivery at pH 3.0 was much larger than cathodal and passive delivery, due to the positive charge of calcitonin. Cumulative amount delivered (CUM) by cathodal or passive delivery was close to zero for 10 hours. The pretreatment of skin by neat IPM markedly increased the CUM anodically. CUM increased as the current density, drug concentration or the duration of IPM treatment increased. Microemulsion containing IPM or oleic acid was prepared and the phase diagram was constructed. CUM also increased when IPM was incorporated into a microemulsion. OA microemulsion showed similar enhancing effect to IPM microemulsion. The delivery of calcitonin from 70% (v/v) ethanol aqueous solution showed a large increase in flux. Laser treatment of skin before flux experiment exhibited about 2 fold increase in total calcitonin amount transported for 12 hours, when compared to that delivered by IPM microemulsion. Based on these results, we have evaluated the possibility of delivering enough amount of calcitonin to reach the therapeutic level. The data suggest that it is highly possible to deliver clinically effective amount of calcitonin using iontophoresis patch with small area (<10 $cm^2$).

• Journal of Pharmaceutical Investigation
• /
• v.40 no.2
• /
• pp.91-100
• /
• 2010

The objective of this work is to study transdermal delivery of donepezil hydrochloride (DH) using iontophoresis and to evaluate various factors which affect the transdermal transport. After the flux study using 4 kinds of hydrogel, hydrogel containing 8% poly(ethylene oxide) (PEO) was chosen as the hydrogel for further studies. Under experimental condition, DH was stable. We have studied the effect of polarity, current density, drug concentration and current profile on transdermal flux and compared the results. In vitro flux study was performed at $33^{\circ}C$, using side-by-side diffusion cell and full thickness hairless mouse skin. DH is positively charged at pH 7.4, and anodal delivery was much larger than cathodal and passive delivery at all current densities studied (0.2, 0.4 and 0.6 mA/$cm^2$). Cathodal delivery showed higher flux than passive flux. Flux increased as the concentration of DH in hydrogel increased. Pulsatile application of current showed smaller flux value than the application of continuous current. Based on these results, we have evaluated the possibility of delivering enough amount of DH to reach the therapeutic level. The maximum cumulative amount of DH transported for 12 hours was 455 ${\mu}g/cm^2{\cdot}hr$ when the amount of DH in the hydrogel was 3 mg/mL and the current density was 0.4 mA/$cm^2$. If the patch size is 10 $cm^2$, then we can deliver 4.6 mg for 12 hours. Because the daily dosage of DH is 5 mg, it seems possible to deliver clinically effective amount of DH using iontophoresis. This study also provides some information about the role of electrorepulsion and electroosmosis during the transport through skin.

• Korean Journal of Environment and Ecology
• /
• v.25 no.3
• /
• pp.389-403
• /
• 2011

Studies on effects of human action on the destruction of ecosystems and changes in biodiversity are being processed actively from the view point of landscape ecology. This study investigated present distribution status of the naturalization plant by disturbance of natural ecosystem which could be seen on 35 rural villages in Korea. The study made a landuse map on the study area then quantified the landscape ecologic characteristic using landscape index based on this landuse map. Correlation between landscape index on the study area and distribution characteristic of naturalization plant was analyzed. The landscape index was calculated by FRAGSTATS, a spatial analysis program, and naturalization index and urbanization index were used in the distribution characteristic of naturalization plant. As a result, naturalization index and patch density, naturalization index and edge density, naturalization index and area-weighted mean shape index were shown as having positive correlation, and naturalization and core area percentage of landscape, naturalization index and mean core area were displayed as having negative correlation.

• Journal of Pharmaceutical Investigation
• /
• v.34 no.6
• /
• pp.491-497
• /
• 2004

We investigated some important factors which affect the transdermal flux of ketoprofen, a nonsteroidal anti-inflammatory agent, as a first step to provide some basic knowledge for the development of a iontophoretic transdermal patch system. Factors such as current density, polarity, buffer (HEPES) and electrolyte concentration and pH were studied using hairless mouse skin. The effect of poly(L-lysin), which is known to affect the electro-osmotic flow through skin, on flux was also studied. Passive flux was about $20\;{\mu}g/cm^2hr$ at pH 4.0, but was negligible at pH 7.4 where all ketoprofen molecules dissolved are ionized (ketoprofen pKa=5.94). At pH 4.0, application of anodal current increased the flux further above the passive level, however anodal flux at pH 7.4 was much smaller than passive flux at pH 4.0. The application of cathodal current at pH 4.0 increased the average flux to $30-40\;{\mu}g/cm^2hr$, depending on the current density applied. At pH 7.4, cathodal flux was only about $5\;{\mu}g/cm^2hr$. Decrease in buffer and electrolyte concentration increased this cathodal flux about 10 fold. However decrease in HEPES buffer concentration 100 fold did not affect the flux. Anodal flux of acetaminophen was much larger than cathodal flux, indicating that electroosmotic flow can be playing an important role in the flux. Poly(L-lysin) increased the cathodal flux at pH 7.4. These results provide some important insights into the mechanism of transdermal flux of ketoprofen and the role of electroosmotic flow.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• v.10 no.10
• /
• pp.4933-4956
• /
• 2016

Use-After-Free (UAF) is a common lethal form of software vulnerability. By using tools such as Web Browser Fuzzing, a large amount of samples containing UAF vulnerabilities can be generated. To evaluate the threat level of vulnerability or to patch the vulnerabilities, automatic deduplication and exploitability determination should be carried out for these samples. There are some problems existing in current methods, including inadequate pertinence, lack of depth and precision of analysis, high time cost, and low accuracy. In this paper, in terms of key dangling pointer and crash context, we analyze four properties of similar samples of UAF vulnerability, explore the method of extracting and calculate clustering eigenvalues from these samples, perform clustering by fast search and find of density peaks on a large number of vulnerability samples. Samples were divided into different UAF vulnerability categories according to the clustering results, and the exploitability of these UAF vulnerabilities was determined by observing the shape of class cluster. Experimental results showed that the approach was applicable to the deduplication and exploitability determination of a large amount of UAF vulnerability samples, with high accuracy and low performance cost.

• Journal of Cadastre & Land InformatiX
• /
• v.47 no.1
• /
• pp.161-178
• /
• 2017

Whereas the demand for development of forested areas covering more than 60% of Korean territory, permission on the forest development has been still given from the perspective of effective land utilization rather than conservation. As the assessment of large forested areas usually focuses more on forest structure, it has its limitation of observing and analyzing the interior change in forest in this way. This study was aimed at computing landscape metrics using a presence vegetation map and FRAGTSTATS 4.2 and analyzing vegetation mosaics. Colonies in native vegetation were classified into a series of major groups and sub-groups based on the native species within the colonies. The colonies were investigated by analyzing a suite of landscape metrics - Core Area, Percentage of Landscape, Number of Patches, Patch Density, Largest Patch Index, Total Edge, Edge Density, Landscape Shape Index, Mean Patch Area, Euclidean Nearest Neighbor. In the Chungnam province major groups and sub-groups of colonies classified based on the proportion of pine and oak species, and pine species was the principal one in terms of distribution area. As for the competition between pines and oaks, while the coverage of pine-centered colonies were three times larger than those of oak-centered ones, pine colonies showed the greater number of patches and therefore higher fragmentation than oaks at the major group level. For the sub-groups, the largest coverage colonies were not only indicated by Pinus densiflora-Quesrcus mongolica colonies among P. densiflora-centered colonies, Q. accutissima colonies among Q. accutissima-centered ones, Q. accutissima-P. densiflora colonies among Q. accutissima-centered ones, Q. mongolica colonies among Q. mongolica-centered ones, P. thumbergii colonies among P. thumbergii-centered ones, and Q. serrata-Q. acutissima colonies among Q. serrata-centered ones, but also revealed more severely mosaicked than other smaller colonies. The overall mosaicking degree estimated by landscape metrics was considered useful for monitoring and investigating vegetation. However, in order to develop management strategy based on analyzing the reason for the mosaicking process and anticipating a trend in vegetation succession, it is essential to further study about ecological characteristics of each colony in the vegetation.

• Journal of Wetlands Research
• /
• v.10 no.3
• /
• pp.9-26
• /
• 2008

To clarify the reed (Phragmites australis) stand's effects on the sediment properties and its increasing pattern, breaths of reed stands in 1999 and 2000, and sediment properties - water, salt, organic matter, sulphur, nitrogen, carbon and hydrogen contents - along th depth at the three stands in 2007 were surveyed at Suncheon Bay. Regardless of reed stand, the more distance from the land was long, the more water and salt contents of sediment were high. Organic matter content of sediment was high and increased with the reed biomass at the upper layer but low regardless of biomass at the lower layer. Sulphur content of sediment was higher at old reed stand (0.33%) than at new stand (0.21%) or non-vegetated stand (0.23%). Carbon, nitrogen and hydrogen contents of sediment were similar at three stands in mean values. However, their contents were high at upper layer and low at lower layer in a stand. Therefore, the changing pattern of organic matter content with the depth was similar to but not coincided to the inorganic nutrients' ones. The mean breadth of reed stands increased $2.33{\pm}0.73m$ in 1999 and $3.65{\pm}1.64m$ in 2000. However, the increase of reed stands' breadth a year varied along the direction, year or stands, so that there was not a trend in increasing pattern. It was thought that this increasing pattern made the reed's patch round. The height and density of reed shoot in newly formed stand decreased with the distance from the center of stand. In the newly formed peripheral area of a reed stand, the shoot density was not related with the land (north) or sea (south) direction, however, the shoot height was higher in the sea (south) than in the land (north) direction.

• Korean Journal of Environment and Ecology
• /
• v.19 no.4
• /
• pp.367-374
• /
• 2005

This study was conducted to comprehend the breeding bird community of thirteen urban forests ranged in size from $0.63{\~}37.0$ ha and compare the species richness of streetscapes such as wooded streets, street trees, and green patches. In urban forests, among thirty-one species observed, two species of Pica pica and Parus major were observed in all study areas, but five species of Phoenicuros auroreus, Pericrocotus divaricatus, Eurystomus orientalis, Emberiza elegans, and Cettia squameiceps were observed only one areas. Number of species was high at urban forests of Manchon, Bonri and Chimsan parks distributed in peripheral area of city, that was low at urban forests Jungri, Igoksaengsu and Sinam parks located in the center area of city. We observed the nests at wooded streets and green patches, did not find at street trees. Number of species and density were significantly high at wooded streets than at street trees. Number of species was highly correlated with the area of forests, and srecies richness of bush-nesting guild was low. As the area of urban forests increase, species richness of bush-nesting and -foraging guild showed the relative low rate of increase than other nesting guild. Our results indicate that bush-nesting and -foraging guild of birds was not being provided with their good habitat at urban forests in Daegu city Among streetscapes, birds preferred to u9e the wooded streets and green patch as habitat and pathway in urban ecosystem.

• Journal of Environmental Impact Assessment
• /
• v.24 no.3
• /
• pp.287-299
• /
• 2015

If the Nakdong River estuary is to be sustainable, land use management practices need to consider bird habitat requirements, especially given that the area serves as an important migratory bird sanctuary. In this study, bird habitats found in the Nakdong River estuary were classified into 11 different types including Phragmites australiss, mud flat, farmland, open surface in freshwater, sand bar, riparian forest, Scirpus planiculmis, waterway, construction, grasslands, and open surface in sea or brackish water. Taking into consideration the regional characteristics, habitat properties, and landscape indices, a total of 12 study sites were analyzed. Mud flat, construction, farmland, and P. australis account for 80% of the total land area. The high area ratio of construction and farmland to other types of habitat revealed a high amount of historical human activity and intervention in the area. Both patch numbers as well as patch density were high in West Nakdong River, Samrak Waterfront, Maekdo River, and Daejeo Floodgate, with these areas showing the greatest fragmentation as well. Total numbers of species and individuals had a positive correlation with the area and the number of habitat types. Findings suggest that protecting the habitat area, especially in S. planiculmis, is the most important factor for bird habitat management and that future development could result in habitat loss, having a profoundly adverse impact on bird populations. Therefore, it is important that the total area should be carefully protected by land use regulations in order to ensure that the Nakdong River estuary maintains its functional integrity as a migratory bird sanctuary.

• Journal of Korean Neurosurgical Society
• /
• v.62 no.5
• /
• pp.594-602
• /
• 2019

Objective : Although surgical intervention, such as percutaneous vertebroplasty (PVP), is the standard treatment for osteoporotic vertebral compression fractures (OVCFs), its effectiveness and safety are unclear. Therefore, this study compared the safety and efficacy of conservative treatment with that of PVP for acute OVCFs. Methods : Patients with single-level OVCFs who were treated conservatively with a transdermal fentanyl patch (TFP) or with PVP between March 2013 and December 2017 and followed-up for more than 1 year were retrospectively evaluated. Patients with pathologic fractures, fractures of more than two columns, or a history of PVP were excluded. Clinical outcomes (visual analog scale [VAS] scores) and radiographic factors were evaluated, including changes in the compression rate of the corresponding vertebral body at onset and after 12 months, sagittal Cobb angle at onset and after 6 and 12 months, and the incidence of adjacent compression fractures. Results : Of the 131 patients evaluated, 75 were treated conservatively using TFPs and 56 underwent PVP. We divided the patients into TFP and PVP groups. Their baseline characteristics (including sex, level of fracture, and bone mineral density T-scores) were similar, but the TFP group was significantly younger. The overall VAS score for pain showed a greater decrease during the first month (1 week after PVP) in the PVP group but remained similar in the two groups thereafter. The compression rate after 12 months increased in the TFP group but decreased in the PVP group. Five patients in the PVP group, but none in the TFP group, experienced adjacent compression fractures within 12 months. Conclusion : We compared clinical and radiological outcomes between the TFP and PVP groups. The immediate pain reduction effect was superior in the PVP group, but the final clinical outcome was similar. Although the PVP group had a better-preserved compression rate than the TFP group for 1 year, the development of adjacent fractures was significantly higher. Although TFPs seemed to be beneficial in reducing the failure rate of conservative treatment, the possibility of side effects (22.6%, 17 out of 75 patients, in this study) should be carefully monitored.