• Natural Product Sciences
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• v.25 no.4
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• pp.348-353
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• 2019

Soluble epoxide hydrolases (sEH) are enzymes present in all living organisms, metabolize epoxy fatty acids to 1,2-diols. sEH in the metabolism of polyunsaturated fatty acids plays a key role in inflammation. In addition, the endogenous lipid mediators in cardiovascular disease are also broken down to diols by the action of sEH that enhanced cardiovascular protection. In this study, sEH inhibitory guided fractionation led to the isolation of five phenolic compounds trans-resveratrol (1), trans-piceatannol (2), sulfuretin (3), (+)-balanophonin (4), and cassigarol E (5) from the ethanol extract of the seeds of Passiflora edulis Sims cultivated in Vietnam. The chemical structures of isolated compounds were determined by the interpretation of NMR spectral data, mass spectra, and comparison with data from the literature. The soluble epoxide hydrolase (sEH) inhibitory activity of isolated compounds was evaluated. Among them, trans-piceatannol (2) showed the most potent inhibitory activity on sEH with an IC₅₀ value of 3.4 μM. This study marks the first time that sulfuretin (3) was isolated from Passiflora edulis as well as (+)-balanophonin (4), and cassigarol E (5) were isolated from Passiflora genus.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.78-78
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• 2018

패션프루트(Passiflora edulis)는 브라질이 원산지이며 아열대와 열대지역에서 재배되고 있는 다년생 상록덩굴식물로 시계꽃과(Passifloraceae) 시계꽃속(Passiflora)에 속한다. 전 세계적으로 500여종이 분포하고 그 중 10여종은 식용, 나머지는 관상용으로 이용되고 있다. 지구온난화로 인한 기온상승으로 전남지역 재배에 적합한 대체 아열대과수 개발이 필요하다. 패션프루트는 겨울 최저온도가 $3^{\circ}C$이상 조건에서 재배 가능하여 남부 지역는 무가온 하우스재배를 하고 있지만 시설내 6월 하순부터 8월 중순까지 $35^{\circ}C$ 이상 고온이 지속되어 과실을 볼 수 없다. 8월 하순부터 피는 꽃은 인공수분으로 착과되어 11월부터 수확이 가능하지만 이후에 낮은 온도($10^{\circ}C$이하)로 착색이 진행되지 않아서 동해피해로 수확을 포기해야 한다. 어느 시기까지 인공수분을 해야 당년에 안정적인 수확이 가능한지를 알아보고자 패션프루트의 후기(2차) 개화시기 중에서 8월 30일, 9월 10일, 9월 20일, 9월 30일에 인공수분 하여 11월 하순에 착색이 정상적으로 진행되는 처리를 조사한 결과 8월 30일 처리구만 11월 21일 착색이 되었다. 미착색과에서 내용물이 진노랑색으로 진행되면 후숙하여 상품과로 판매가 가능하기 때문에 진노랑색에 도달한 시기를 조사하였다. 조사에서 9월 10일까지 인공수분 처리한 과실은 후숙하여 상품과로 판매가 가능하였다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2021.04a
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• pp.39-39
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• 2021

지구온난화에 따른 기후변화로 과거에는 재배되지 않았던 아열대 작물의 국내재배가 확대되고 있다. 백향과(Passiflora edulis Sims)는 브라질 남부지역이 원산지인 아열대작물로 국내에서도 전국적으로 재배가 되고 있어서, 국내 재배현황과 재배농가의 관리기술 실태를 조사하여 재배 애로사항 및 발전과제를 도출코져 하였다. 백향과의 국내 재배는 2010년부터 시작되어 2017년에는 201농가 54.7ha로 정점을 이루고, 점차 감소하여 2019년에는 156농가 36.5ha에서 재배되고 있다. 재배농가가 많았던 지역은 남원, 담양, 화순, 김천, 고창 등 이었다. 백향과의 재배체계는 대부분의 농가가 비닐하우스 시설에서 겨울철 난방에 의한 다년 재배로 1년 2회 수확하고 있으며, 극히 일부농가는 1년 재배체계로 매년 묘목을 다시 심는 형태이다. 백향과의 국내 육성품종은 없으며, 외국에서 도입한 자색종 또는 교잡종을 주로 재배하고 있다. 현재 국립종자원에 '일반종', '타이농1하오', '황금' 3품종이 생산/수입 판매 신고되어 있다. 초기에 도입한 접목묘를 재배하면서 국내에서 삽목묘를 육성하여 많은 농가가 재배하고 있다. 백향과는 덩굴성이기 때문에 지주를 설치하여 재배하는데 재식거리, 수형, 가지유인 등을 농가별로 다양한 방법으로 하고 있는 실정이다. 개화기에 인공수분 작업이 필요하고, 수확은 과일이 성숙하여 저절로 낙과하면 주어서 수확한 과일은 주로 생과로 판매하는데 전화 및 인터넷 주문에 의한 직거래가 가장 많고, 일부 마트 및 로컬푸드에 판매하고 있다. 국내재배 안전성 향상을 위하여 필요시 되는 기술개발 과제는 적응 품종육성 및 무병묘 생산 보급, 고품질 백향과 생산을 위한 재배관리 기술, 시설재배 환경관리 기술, 소비 증대를 위한 가공 이용 기술 개발 등으로 전문기관에서의 연구개발이 필요하다.

• Research in Plant Disease
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• v.30 no.1
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• pp.60-65
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• 2024

In July 2020, total RNA was extracted from passion fruit (Passiflora edulis) leaves showing virus symptoms such as chlorotic spots and vein banding in Haenam, South Korea. Cucumber mosaic virus (CMV)-HN2 was identified through reverse transcription polymerase chain reaction and sequencing analysis. To confirm the biological characteristics of the CMV infecting passion fruit, 10 indicator plants were inoculated with CMV-HN2, and the results showed a typical CMV symptoms. Phylogenetic analysis based on the amino acid of the coat protein (CP) of CMVs revealed that the CMV passion fruit isolates belonged to subgroup I, among which CMV-HN2 belonged to subgroup IA. Additionally, CMVs isolated from passion fruit in Korea have amino acid sequence variation between the subgroup. Among them, CMV-HN2 had four to eight amino acid differences in CP from other CMV isolates from passion fruit. These results confirm the presence of genetic diversity in the CPs of passion fruit CMV isolates.

• Journal of Plant Biotechnology
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• v.44 no.3
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• pp.335-342
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• 2017

Purple passion fruit (Passiflora edulis Sims) is one of the introduced tropical plants, an increasing interest has arisen due to its distinctive taste and attractive flavor. It is expected that passion fruit production and planted area will increase gradually in the years ahead because of high profitability and consumer's demands of healthful ingredients. So we tried to investigate the effect of plant growth regulators and antioxidants on in vitro plant regeneration and callus induction from leaf explants of passion fruit for an establishment of optimal mass propagation system. Young leaf explants of purple passion fruit were cultured in Murashige and Skoog (MS) medium containing different growth regulators and antioxidant additives to induce the shoot organogenesis. After 8 weeks, the highest embryogenic callus formation rate was obtained in MS medium supplemented with $1mg{\cdot}L^{-1}$ 6-benzylaminopurine (BAP) and $2mg{\cdot}L^{-1}$ 2,4-dichlorophenoxyacetic acid (2,4-D), furthermore, the shoot development via organogenesis was also observed. Silver nitrate ($AgNO_3$), which was added into the medium to minimize the adverse effects of leached phenolics, was effective for reduction of medium browning and sudden explant death. In the medium supplemented with $1mg{\cdot}L^{-1}$ BAP and $1mg{\cdot}L^{-1}$ gibberellic acid ($GA_3$), shoots were most vigorously regenerated and elongated. Most shoots rooted successfully in half strength medium with $1mg{\cdot}L^{-1}$ indol-3 acetic acid (IAA), and more than 90% of plantlets survived after 4-month acclimatization period.

• Preventive Nutrition and Food Science
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• v.15 no.1
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• pp.57-66
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• 2010

Passion fruit fiber pectin gels represent a new alternative pectin source with potential for food and non-food applications on a commercial scale. Pectic polysaccharides were extracted from passion fruit (Passiflora edulis) fiber using citric acid as a clean catalyst and autoclaved for 20 to 60 min at $121^{\circ}C$. The best condition of pectin yield with the highest molecular weight was obtained with 1.0% of citric acid (250 mg/g dry passion fruit fiber pectin) for 20 min of autoclaving. Spectroscopic analyses by Fourier transform infrared, enzymatic degradation reactions, and ion-exchange chromatography assays showed that passion fruit pectin extracted for 20 min was homogeneous high methoxylated pectin (70%). Gel permeation analysis confirmed that the pectin extract obtained by autoclaving by 20 min showed higher molecular weights than those autoclaved for 40 and 60 min. Passion fruit pectin extracted for 20 min was enzymatically modified with fungal pectinmethylesterase to create restructured gels. Short autoclave treatment (20 min) with citric acid as extractant resulted in a significant increase of gel strength, improving pectin extraction in terms of functionality. The treatment of solubilized material (pectic polysaccharides) in the presence of insoluble material (cellulose and hemicellulose) with pectinmethylesterase and calcium led to the creation of a stiffer passion fruit fiber pectin gel, while syneresis was not observed.

• Horticulture, Environment, and Biotechnology : HEB
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• v.59 no.6
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• pp.847-856
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• 2018

This research aimed to evaluate the effectiveness of hot water ($43-53^{\circ}C{\cdot}5min^{-1}$; $47^{\circ}C{\cdot}2-6min^{-1}$), 1-methylcyclopropene (1-MCP) at $50-300nL\;L^{-1}$ and a combination of hot water ($47/49^{\circ}C{\cdot}5min^{-1}$) and phosphite $40%\;P_2O_5+20%\;K_2O$;$40%\;P_2O_5+10%\;Zn$) in anthracnose control and the effect on fruit quality [fresh weight loss (FWL-%); pH, total soluble solids ($TSS-^{\circ}Brix$), and titratable acidity (TA = % citric acid (CA)] of passion fruit ( Passiflora edulis f. flavicarpa ) at the postharvest stage. When the fruits were in the stage of 0% dehydration and fully yellow peels, they were disinfested and inoculated with Colletotrichum gloeosporioides. They were then subjected to the above mentioned treatments; this was followed by incubation for 120 h. The diameter of the disease lesions was monitored daily. After the incubation, a physico-chemical analysis was performed. Hot-water treatment resulted in disease reduction at 47 and $49^{\circ}C$ for 4 and 5 min. The combination of hot-water treatment at $47^{\circ}C$ (4 or 5 min) and application of the phosphite of K or Zn significantly reduced disease severity in fruits. The 1-MCP treatment reduced anthracnose severity in passion fruit mainly at $200nL\;L^{-1}{\cdot} 24h^{-1} $. None of the treatments significantly changed the physico-chemical characteristics of the fruit [FWL (2.6-4.1%); pH (3.2-3.5), TSS ($8.9-10.9^{\circ}Brix$), and TA (1.8-2.5% CA)].

• Natural Product Sciences
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• v.16 no.1
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• pp.43-49
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• 2010

Thirty-two methanol extracts of thirty-one Vietnamese medicinal plants were evaluated for the cytotoxic activity against five human cancer cell lines, including A549, MCF-7, HT 1080, Huh-7, and HepG2. Of these, the nine extracts of Acanthopanax trifoliatus (4), Acanthopanax gracilistylus (5), Siegesbeckia orientalis (10), Betula alnoides (11), Passiflora edulis (18), Zanthoxylum simulans (leaf, 23), Adenosma caeruleum (26), Solanum verbascifolium (29), and Alpinia malaccensis (31), exhibited high potent cytotoxic activity showing a certain degree of selectivity against the different cell types, with $IC_{50}$ values ranging from 2.1 to $3.8\;{\mu}g/mL$.

• Mycobiology
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• v.34 no.2
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• pp.92-98
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• 2006

A total of 34 species belonging to 21 genera of fungi were recorded on passion fruits of both pure and hybrid origin in Uganda, however, the pure type exhibited wider spectrum (28 species and 16 genera) than the hybrid type (21 & 15). Also, yeasts (unidentified and Rhodotorula mucilaginosa) were also encountered in high numbers. Moreover, the mean count of all mycobiota obtained from the pure type was higher than that of hybrid, despite the bigger size of the later. Members of yeasts and Cladosporium followed by Phoma, Penicillium, Fusarium and Alternaria species dominated on passion fruits of pure origin, while only C. cladosporioides, F. solani and yeasts dominated on the hybrid type. Treatment with Na-hypochlorite exhibited inhibitory effects on the total mycobiotic propagules as well as the dominant species from fruits of both types. The current results, therefore, suggest the use of Na-hypochlorite to control the post-harvest mycobiota associated with passion fruits. Regarding the mycobiota contaminating passion juice, yeasts were found to be the major contaminants with Candida parapsilosis being the most common. Moulds constituted only a minor proportion with Acremonium strictum followed by Fusarium chlamydosporum, F. moniliforme, F. acuminatum and F. solani as the most dominant species. In the heat-treated juice samples, the counts of the most commonly encountered mycobiota (both yeasts and molds) were significantly inhibited or completely eliminated. Some unidentified Bacillus species were also recovered from the juice, however, their counts in the heated samples were increased but insignificantly.

• Research in Plant Disease
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• v.24 no.1
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• pp.75-80
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• 2018

From 2014 to 2016, Fusarium wilt disease was found on fassionfruit in Iksan and Jeju, Korea. Symptoms included wilting of foliage, drying and withering of leaves, and stunting of the plants. The infected plants eventually died during growth. Colonies on potato dextrose agar were pinkish white, and felted with cottony and aerial mycelia with 35 mm after one week. Macroconidia were falcate to almost straight, thin-walled and usually 2-3 septate. Microconidia were usually formed on monophialides of the hyphae and were hyaline, smooth, oval to ellipsoidal, aseptate or medianly 1-septate, very occasionally 2-septate, slightly constricted at the septa, $3-12{\times}2.5-6{\mu}m$. On the basis of the morphological characteristics and phylogenetic analyses of two molecular markers, internal transcribed spacer rDNA and translation elongation factor $1{\alpha}$, the fungus was identified as Fusarium oxysporum. Pathogenicity of a representative isolate was proved by artificial inoculation, fulfilling Koch's postulates. To our knowledge, this is the first report on the occurrence of F. oxysporum on fassionfruit in Korea.