• Genomics & Informatics
• /
• v.13 no.3
• /
• pp.81-85
• /
• 2015

Molecular characterization technology in genetically modified organisms, in addition to how transgenic biotechnologies are developed now require full transparency to assess the risk to living modified and non-modified organisms. Next generation sequencing (NGS) methodology is suggested as an effective means in genome characterization and detection of transgenic insertion locations. In the present study, we applied NGS to insert transgenic loci, specifically the epidermal growth factor (EGF) in genetically modified rice cells. A total of 29.3 Gb (${\sim}72{\times}coverage$) was sequenced with a $2{\times}150bp$ paired end method by Illumina HiSeq2500, which was consecutively mapped to the rice genome and T-vector sequence. The compatible pairs of reads were successfully mapped to 10 loci on the rice chromosome and vector sequences were validated to the insertion location by polymerase chain reaction (PCR) amplification. The EGF transgenic site was confirmed only on chromosome 4 by PCR. Results of this study demonstrated the success of NGS data to characterize the rice genome. Bioinformatics analyses must be developed in association with NGS data to identify highly accurate transgenic sites.

• The Korean Journal of Community Living Science
• /
• v.22 no.3
• /
• pp.327-339
• /
• 2011

This study aims to investigate relationships between benefit sought and selection attributes of green tea consumers. For data collection, a total of 595 copies of questionnaires were collected by convenience sampling in the Seoul and Gyeonggi-do area. The data were analyzed by using SPSS 15.0. The factor analysis identified four dimensions of the benefit sought : health benefit, sensory, sociality, and self-esteem. Six dimensions of selection attributes were identified as manufacturing, design, sensory appeal, recommendation, utility and brand. The results of the canonical correlation analysis indicated that health benefit, sensory, sociality of benefit sought and manufacturing, design, sensory appeal, recommendation, utility, brand of selection attributes were highly correlated, and the self-esteem of benefit sought and recommendation of selection attribute were highly correlated. This means it is important to place an emphasis on safety production, package design, sensory characteristics, product description, utility and brand for consumers who seek health benefit, flavor and sociality. It is also important to place an emphasis on product description for consumers who pursue self-esteem benefits. Green tea marketers should consider benefit sought aspects as the most important factors affecting selection attributes on green tea purchasing.

• 한국해양학회지
• /
• v.20 no.3
• /
• pp.30-36
• /
• 1985

Water quality of seawater samples from ten stations was measured and analyzed to study the causes and the characteristics of the yellowish-green-colored zone in the Nakdong Estuary in February, May, August and October, 1984. The color of the yellowish-green-colored zone was equivalent to the grade 7 according to the Forel water-color meter and was observable throughout the year. The characteristics of the zone were low in transparency and high in the concentration of suspended solids, and about 52% of the suspended solids consisted of volatile substances. The chlorophyll a contents ranged from 0.4mg/$m^3$ to 51.0mg/$m^3$ and dissolved oxygen was in a state of supersaturation within the yellowish-green-colored zone. Nutrient concentrations were higher within the colored zone than the outside. Positive correlation was observed between tne concentrations of nutrients and chlorophyll a. Our results showed that the signficant portion of suspended solids sampled from the yellowish-green-colored zone consisted of phytoplanktons of which bloom was possibly induced by inflow of the nutrient-rich Nakdong River water.

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.16 no.6
• /
• pp.109-117
• /
• 2013

This study was carried out to investigate the changes of plant species and soil physicochemical properties on green roofs established at Seoul Women's University in 2005, 2006 and 2007. The plant species and soil properties were investigated in 2013. The areas of green roof sites ranged $90{\sim}100m^2$. There were floras of vascular plants of 12 families, 20 genera and 22 species in the 2005 site, 24 families, 37 genera and 38 species in the 2006 site, 14 families, 27 genera and 31 species in the 2007 site. The total number of plant species decreased in the 2005 and 2006 sites and increased in the 2007 site since established. High proportion of dispersal type was barochory in the 2005 and 2006 site, and autochory in the 2007 site. And the proportion of the compositae family was high in the introduced plants over the sites for the all study sites. Average pH and organic matter concentration of green roof soil were ranged from 5.25 to 5.96 and 7.17 to 8.96% in study sites. The organic matter concentration and pH of green roof soil were lower in 2013 than in the three establishment years. Carbon concentration of green roof soil in the three study sites were ranged from 4.16 to 5.30% and total soil carbon in 10cm depth were ranged form 1.57 to $1.98kg/m^2$.

• Korean Journal of Food Science and Technology
• /
• v.31 no.2
• /
• pp.488-494
• /
• 1999

Changes of lactic acid bacteria and fermentation patterns were investigated during fermentation of Green Onion Kimchi and Chinese Cabbage Kimchi at 10 and $20^{\circ}C$. Fermentation of Green Onion Kimchi proceeded more slowly than Chinese Cabbage Kimchi in point of changes of pH, total acidity and total viable cell number. Maximum number of total viable cell, Leuconostoc, and Lactobacillus in Green Onion Kimchi were smaller than in Chinese Cabbage Kimchi. And these differences were larger in fermentation at $10^{\circ}C$. Total sugar content of Green Onion Kimchi was higher than Chinese Cabbage Kimchi not only at the beginning of fermentation but also at the end of fermentation. Therefore, the reason for the slow fermentation of Green Onion Kimchi was not low sugar content. Major lactic acid bacteria of properly fermented Green Onion Kimchi were identified as Lactobacillus plantarum and Leuconostoc mesenteroides which had been reported to be major lactic acid bacteria isolated from Chinese Cabbage Kimchi.

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.25 no.1
• /
• pp.25-38
• /
• 2022

Urban problems are constantly occurring around the world due to rapid industrialization and population decline. In particular, as the number of vacant houses is gradually increasing as the population decreases, it is necessary to prepare countermeasures. A plan to utilize vacant houses has emerged to restore the natural environment of the urban ecosystem where forest destruction, damage to habitats of wild animals and plants, and disconnection have occurred due to large-scale development. Through connectivity analysis, it is possible to understand the overall ecosystem flow based on the movement of species and predict the effect when vacant houses are converted into green spaces. Therefore, this study analyzed the green area network to confirm the possibility of greening of vacant houses neglected in Jeonju based on circuit theory. Using Circuitscape and Least-cost path, we tried to identify the connectivity of green areas and propose an ecological axis based on the analysis. In order to apply the resistance values required for analysis based on previous studies, the 2020 subdivision land cover data were integrated into the major classification evaluation items. When the eight forests in the target site were analyzed as the standard, the overall connectivity and connectivity between forests in the area were high, so it is judged that the existing green areas can perform various functions, such as species movement and provision of habitats. Based on the results of the connectivity analysis, the importance of vacant houses was calculated and the top 20 vacant houses were identified, and it was confirmed that the higher the ranking, the more positive the degree of landscape connectivity was when converted to green areas. In addition, it was confirmed that the results of analyzing the least-cost path based on the resistance values such as connectivity analysis and the existing conceptual map showed some differences when comparing the ecological axes in the form. As a result of checking the vacant houses corresponding to the relevant axis based on the width standards of the main and sub-green areas, a total of 30 vacant houses were included in the 200m width and 6 vacant houses in the 80m width. It is judged that the conversion of vacant houses to green space can contribute to biodiversity conservation as well as connectivity between habitats of species as it is coupled with improved green space connectivity. In addition, it is expected to help solve the problem of vacant houses in the future by showing the possibility of using vacant houses.

• Journal of Korean Society of Rural Planning
• /
• v.19 no.3
• /
• pp.37-50
• /
• 2013

Since 2010, RDA has discovered 30 Green-Road as the way of developing rural area. However, results from conference with expert and village leaders of Green-Road concluded that there were problems such as little financial benefits due to mismanagement and needs of improving the efficiency of management. In order to respond to these issues, this research has conducted empirical investigation about the status, management of Green-Road and its influences on local area. Main purposes of this investigation was to reveal the various obstacles in road management, and to find better ways of road management. To accomplish the goal of research, this study conducted survey and interviews with leaders in villages. Particularly, the investigation had focused on the specific management issues suggested by village leaders. Based on the results, this research suggested establishing a committee for creating leaders network and developing effective management method in order to improve road management and its consequences.

• Korean Journal of Organic Agriculture
• /
• v.19 no.spc
• /
• pp.153-156
• /
• 2011

Supplying rate of nitrogen at HV was 172.8 kg $ha^{-1}$, HV/B was 64.3 kg $ha^{-1}$ and B was 38.6 kg $ha^{-1}$. The Rice yield was 7.05 ton $ha^{-1}$ when the nitrogen supply was the largest with HV and 5.42 ton $ha^{-1}$ was produced on HV/B. The chemical characteristics of soil have lower pH and exchangeable cations(Ca and Mg) at B, HV and HV/B, rather than at CF because green manure was applied at the former step. However, the physical characteristics of the soil and the porosity showed different tendency which was that it was better at the green manure crops than CF. Nitrogen nutrient balance was showed the most balanced at CF and field of application of green manure crops were required the appropriate management if future crops would be cultivated because nitrogen nutrient could be exhausted or accumulated.

• Journal of Korean Society of Rural Planning
• /
• v.15 no.4
• /
• pp.135-146
• /
• 2009

Urban forest area in South Korea has been increased up to 20.8% of national land as of 2007 by the mergence of municipalities and counties. The rate of park and green tract among urban forest averages out to about 4% nationally but the Capital, Seoul, almost 105% level, not easy to split the two. Park and green has positive management system because of its infrastructure attribute and relevant security obligation standard while urban forest left alone with no such concerns. The most important reason of the negligence comes from institutional inertia although it is possible to be managed functionally by forest laws as like forests of park, landscape, wind and noise protection, and timber product. As a results, it reaches below than 92.64% of the national average level of the timber stockpile especially in the metropolitan areas and loses broad acres rapidly due to the easy conversion system to the urban land usage. Therefore, there must be required some alternative methodologies to conserve and foster it. The paper proposes the four urban forest management types and their control methods. The four types divide into conservation, foster, development, and reservation ones. Also it suggests the five furtherance tract types with the combination among the four earlier types, optionally having one of the six aim climaxes and recommends the standardization of operation design. The total green quantity index of urban forest also suggested by the weight method according to location and DBH class. The case study of the suggested model was executed on the Daejon Metropolitan area and its index calculated as 110.4% level compared with the acreage.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
• /
• v.28 no.2
• /
• pp.142-147
• /
• 2015

The artificial light sources for growth of plant are usually high-pressure sodium lamp, metal haloids lamp, and fluorescent light; however, these light sources have relatively weaker Red and Blue lights that are necessary for growth of plants. Especially the effect of Photosynthetic Photon Flux Density (PPFD) is pointed out as the weakness. Meanwhile, LED light source can be selected by specific wavelength to greatly improve the effect of PPFD. In this regard, this paper aims to investigate the promotion of plant growth by measuring photosynthetic photon flux density (hereafter referred to as PPFD) according to changes in light quality of the LED light sources. Towards this end, LED light sources for plant growth were produced with 4 kinds of mono-chromatic lights and 6 kinds of combined lights by mixing red, blue, green and white lights. A comparative analysis was conducted to investigate the effects of optical properties and PPFD on plants (green leaf lettuce) using the produced light sources. The results monochromatic light has fastest growth rate, but plant growth conditions have poor. This being so, mixed light is suitable for the green leaf lettuce.