• Asian-Australasian Journal of Animal Sciences
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• v.31 no.6
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• pp.791-797
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• 2018

Objective: Trimethylation of histone 3 (H3) at 4th lysine N-termini (H3K4me3) in gene promoter region was the universal marker of active genes specific to cell lineage. On the contrary, coexistence of trimethylation at 27th lysine (H3K27me3) in the same loci-the bivalent H3K4m3/H3K27me3 was known to suspend the gene transcription in germ cells, and could also be inherited to the developed stem cell. In galline species, throughout example of H3K4m3 and H3K27me3 ChIP-seq analysis was still not provided. We therefore designed and demonstrated such procedures using ChIP-seq and mRNA-seq data of chicken follicular mesenchymal cells and male germ cells. Methods: Analytical workflow was designed and provided in this study. ChIP-seq and RNA-seq datasets of follicular mesenchymal cells and male germ cells were acquired and properly preprocessed. Peak calling by Model-based analysis of ChIP-seq 2 was performed to identify H3K4m3 or H3K27me3 enriched regions ($Fold-change{\geq}2$, $FDR{\leq}0.01$) in gene promoter regions. Integrative genomics viewer was utilized for cellular retinoic acid binding protein 1 (CRABP1), growth differentiation factor 10 (GDF10), and gremlin 1 (GREM1) gene explorations. Results: The acquired results indicated that follicular mesenchymal cells and germ cells shared several unique gene promoter regions enriched with H3K4me3 (5,704 peaks) and also unique regions of bivalent H3K4m3/H3K27me3 shared between all cell types and germ cells (1,909 peaks). Subsequent observation of follicular mesenchyme-specific genes-CRABP1, GDF10, and GREM1 correctly revealed vigorous transcriptions of these genes in follicular mesenchymal cells. As expected, bivalent H3K4m3/H3K27me3 pattern was manifested in gene promoter regions of germ cells, and thus suspended their transcriptions. Conclusion: According the results, an example of chicken H3K4m3/H3K27me3 ChIP-seq data analysis was successfully demonstrated in this study. Hopefully, the provided methodology should hereby be useful for galline ChIP-seq data analysis in the future.

• Journal of architectural history
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• v.7 no.1 s.14
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• pp.93-108
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• 1998

The threads of this thesis are several theoretical issues of modern urban ideals. Modern architects and urban designers conceived their individual artifacts, which assumed to be laid out on the new settings totally different from the existing urban fabrics derived from inherently medieval ones. In the discussion of modern ideal society, the Industrial Revolution and the Enlightenment was a pivotal point. Innovations in technology and expanded living territories since the double revolution have been critical factors in the evolution of new ideas of urbanism. The tremendous success in science and technology led a way to the 'science-fiction' environment as a destined apocalyptic world. The dream, whether it was socialist or in any other believes, to a pastoral utopia beyond the capitalist society was represented through the ideal cities, which were modern versions of arcadia in the other approaches. Two sides of revolutionary ideas are presented as a futurist city and a garden city, which are on the separate notions but co-existed or overlapped in a single urban project such as in Le Corbusier urban schemes or even Tchumi's recent work, Parc de la Villette. Urban ideas in the twentieth century are based on urban naturalism, the notion of which was consistant from abbe Laugier to Le Corbusier, as well as machine aesthetics interpreted in terms of archeological research and modern technology.

• Proceedings of the Korean Vacuum Society Conference
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• 2014.02a
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• pp.91-91
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• 2014

2002년 21세기 프론티어 연구개발사업으로 착수한 양성자기반공학기술개발사업이 2012년말 완료되었다. 이 사업을 통하여 이온원, RFQ, DTL, 초전도 가속관, 빔라인, 고주파 시스템, 제어 시스템 등 양성자 및 이온 가속기의 핵심 요소기술을 개발하고 100 MeV 선형 양성자가속기 및 이온가속기를 제작하여 경주에건설한 양성자속기연구센터에 설치하고 사업을 마무리하였다. 2013년 상반기에 냉각시스템 등 부대시설의 시운전, 양성자가속기와 20 MeV 및 100 MeV 빔라인 각 1기를 포함한 모든 시설의 시운전을 마무리하고, 한국원자력안전기술원의 방사선안전시설검사를 거쳐 7월부터 운영을 착수하였다. 양성자가속기는 2013년말 까지 총 2,290 시간을 가동하여 937건의 이용자 빔 서비스를 제공하였다. 기체, 금속, 대전류 이온을 공급할 수 있는 이온가속기 3대는 기업체의 공정 및 제품개발을 위한 이용을 중심으로 622건의 이용자 빔 서비스를 제공하였다. 2014년도는 양성자가속기는 연간 2,500 시간 가동, 빔 서비스 1,100건을 목표로 하고 있으며, 현재 20 MeV 및 100 MeV 각 1기인 뿐인 빔라인 증설을 준비하고자 한다. 이온가속기는 상반기에만 이용자 빔 서비스를 제공하고 2014년 11월 완공될 빔이용연구동으로 이전 설치하여 보다 양질의 이온빔을 공급할 수 있도록 장치를 보완 할예정이다. 더불어 2015년에는 3 MeV 헬륨빔과 1 MeV 기체 이온빔을 제공할 수 있는 장치도 추가로 설치할 예정이다. 빔이용연구동 및 이온가속기 업그레드가 완료되면 보다 다양한 양질의 이온빔을공급하여, 특히 중소기업의 제품 개발에 도움을 줄 수 있도록노력할 예정이다. 양성자가속기연구센터는 장기 비전인 펄스형 중성자원의 구축을 실현하고자 노력을 지속할 것이다. 미국의 SNS 및 일본의 J-PARC 파쇄 중성자원은우수한 연구 성과를 생산하기 시작하였고 우리나라도 이에 대한 수요가 생겨나도 있다. 충분한 수요가 형성되면 이미 확보한 부지와 초전도 가속기 기술을 활용하여 단시간 내에 펄스형 중성자원 구축이 가능할 것이다. 펄스형 중성자원이 구축되면 양성자가속기연구센터는 당초 목표한 양성자, 중성자 및 다양한 종의 이온을 한 사이트에서 제공하여, 입자빔을 이용한 다양한 연구개발에서 상당한 시너지 효과를 낼 수 있을 것으로 기대한다.

• Journal of the Korea Concrete Institute
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• v.20 no.5
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• pp.549-555
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• 2008

In this paper, the applicability of Hwangtoh, as an alternative of cement paste, is investigated for the solution of internal heat and shrinkage caused by the hydration of cement paste. Several small-sized specimens of Hwangtoh and ordinary portland concrete (OPC) were compared as to compressive strength, heat of hydration, and shrinkage strain. Moreover, the applicability to the construction structures was reviewed through the test of large-size specimens. The 28-day compressive strength of Hwangtoh concrete (HBC), ranged 18 to 33 MPa, can reach that of OPC. Not only the maximun internal temperature of HBC was read about 1/4 of OPC as it is cured, but also its shrinkage decreased more than the OPC did. Therefore, Hwangtoh binder is more favorable than cement binder in terms of its hydration heat and shrinkage under the construction of concrete.

• Asian-Australasian Journal of Animal Sciences
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• v.5 no.2
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• pp.261-266
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• 1992

Yearling replacement heifers were used in a two-year study at the Fort Stanton Experimental Ranch near Capitan, New Mexico, USA to compare frequency (once versus three times weekly) of feeding a cottonseed cake supplement. The study involved periods of 130 days (January 4 through May 14) in 1985, and 146 days (December 4 through April 29) in 1986. In each year, supplemental periods were just before the breeding season (natural breeding in multiple-sire groups) that lasted for 75 days during first year and 60 days during second year. During the first year, all (83) heifers (Angus $\times$ Hereford) were supplemented at a level equivalent to 45 kg/head daily and during second year, all (69) heifers (Angus $\times$ Hereford, Hereford) were fed at a level equivalent to .68 kg/head daily. In the first year, heifers were sorted by initial weight into two replications, i.e., those weighing under 223 kg were placed in replicate 1 and those weighing over 223 kg were placed in replicate 2. During the second year, heifers were sorted by breed into two replications, i.e., Angus $\times$ Hereford heifers were placed in replicate 1 and Hereford heifers in replicate 2. Each year, half of the heifers in each replication were supplemented once weekly and the other half three time weekly. Weight gains of heifers were similar for the two treatments over both years of study. During the first year, weight gains for heifers supplemented once or thrice weekly (.23 vs .21 kg daily, respectively) did not differ (p>.05) and the same trend for the second year (.15 vs .16 kg daily, respectively) was observed. Similarly, breeding performance was consistent with weight gains and no difference (p>.05) between treatments was observed either year. Lower conception rates during the second tear of study (92 vs 72% for years 1 and 2, respectively) were due mainly to involvement of Hereford heifers that had an average weight of 232 kg at the start of breeding season and conception rate of 54% (averaged across both supplemental treatments). Based on the combined results of both trials, frequency of supplementation did not affect growth rate of yearling heifers nor did it influence their subsequent conception rate.

• Restorative Dentistry and Endodontics
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• v.13 no.2
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• pp.221-235
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• 1988

The purpose of this study was to observe characteristic properties through the polarization curves and EMPA images from 4 different types of amalgam obtained by using the potentiostats (EG & G PARC) & EPMA (Jeol JSM-35), to investigate the degree of corrosion of each phase of amalgam on the oxidation peak, and to identify corrosion products from the corroded amalgam by use of X-ray diffractometer(Rigaku). After each amlgam alloy and Hg were triturated as the direction of the manufacturer by means of the mechanical amalgamator(Shofu), the triturated mass was inserted into the cylindrical metal mold which was 12mm in diameter and 10mm in height and was condensed by means of routine manner. The specimen was removed from the mold and stored at room temperature for about 7 days. The standard surface preparation was routinely carried out. Anodic polarization measurement was employed to compare the corrosion behaviours of the amalgams in 0.9% saline solution(pH6.8~7.0) and artificial saliva (pH6.8~7.0) at $37^{\circ}C$. The open circuit potential was determined after 30 minutes' immersion of specimen in electrolyte and the potential scan was begun at the potential of 100mV cathodic from the corrosion potential. The scan rate was 1mV/sec and the surface area of amalgam exposed to the solution was 0.64$cm^2$ for each specimen. All the potentials reported are with respect to a saturated calomel electrode (SCE). EPMA images on the determined oxidation peaks of each amalgam in artificial saliva were observed. X-ray diffraction patterns of each sample were recorded before and after polarization in artificial saliva (Aristaloy, Caulk Spherical, Dispersalloy and Tytin: at +770mV, +585mV, +8.10m V and +680m V respectively) by use of a recording diffractometer. Nickel filtered Cu $K_{{\alpha}_1}$ radiation was used and sample was scanned at $4^{\circ}(2{\theta})/min.$ from $25^{\circ}$ to $80^{\circ}$. The following results were obtained. 1. Oxidation peak potential in artificial saliva shifted to more anodic direction than that in saline solution. 2. The corrosion potential of high copper amalgam was more anodic than the potential of low copper amalgam. 3. The current density was lower in artificial saliva than in saline solution. 4. One of the corrosion products, AgCl was identified by X-ray diffraction analysis. 5. ${\gamma}_2$ phase was the most susceptible to corrosion and e phase was stable in low copper amalgam and ${\eta}$' phase and Ag-Cu eutectic were susceptible to corrosion in high copper amalgam.

• Korean Journal of Dental Materials
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• v.44 no.2
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• pp.151-161
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• 2017

The purpose of this study was to examine the effect of shade and thickness of resin-nanoceramic CAD-CAM block (RNB) on the microhardness of dual-cured resin cement, as well as to measure the number of photons transmitted through RNBs of different thicknesses and colors. One dual-cured resin cement was used to prepare resin cement specimens. Resin cement specimens were light-cured for 40 seconds through 3 shades (A1, A2, A3 in HT (high translucency) and LT (low translucency) respectively) and four thicknesses (1, 2, 3, 4 mm) of RNB specimens. Vickers microhardness measurements of resin cement specimens were performed using a Vickers hardness tester. The light transmission of RNB specimens was measured using a spectrometer (SpectroPro-500, Acton Research, Acton, MA, U.S.A.), and the translucency parameter was calculated using the CIEL*a*b* system. Data were statistically analyzed by ANOVA and Tukey's test. There was a significant decrease of microhardness of resin cement specimen with an overlay of 4 mm of RNB thickness and A3 shade in comparison to A1 and 1 mm, respectively (p<0.05). The translucency parameter values and light transmission of RNBs tested differed significantly, according to the thicknesses of the specimen (p<0.05). Light transmission is decreased with increase in the thicknesses of RNBs. Shade A1 transmitted more light than darker blocks. A decrease in microhardness of resin cement specimens was observed with increasing thickness and shade (A1 to A3) of RNBs.