• Journal of Ginseng Research
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• v.32 no.2
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• pp.107-113
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• 2008

In this study, we have investigated the effect of panaxatriol (PT) on phosphoinositides (PIS) breakdown and $Ca^{2+}$-elevation in thrombin-induced platelet aggregation. Thrombin (5U/ml), a potent platelet agonist which activates phospholipase $C_{\beta}$ via protease activated receptor (PAR), hydrolyzed PIS in platelet membrane. The phosphatidylinositol 4, 5-bisphosphate $(PIP_2)$ was hydrolyzed after 10 sec of the thrombin-stimulation, and both the phosphatidylinositol 4-monophosphate (PIP) and phosphatidylinositol (PI) were brokendown after 30 sec of the thrombin-stimulation. However, PT inhibited the thrombin-stimulated hydrolysis of $PIP_2$, PIP, and PI. On the other hand, thrombin increased the level of phosphatidic acid (PA) which is phosphorylated from diacylglycerol (DG) generated by PIS-hydrolysis. However, Pr inhibited the thrombin-increased PA level non-significantly. Thrombin increased cytosolic free $Ca^{2+}([Ca^{2+}])_i$) up to 72% as compared with control $(30.8{\pm}0.9 nM)$ in intact platelet. However, PT (100 ${\mu}g/ml$) inhibited the thrombin-elevated $[Ca^{2+}]_i$ to 100%. These results suggest that PT may have a beneficial effect on platelet aggregation-mediated thrombotic disease by inhibiting thrombin-induced platelet aggregation via suppression of the $[Ca^{2+}]_i$ level and PIS breakdown.

• Journal of Korean Society of Forest Science
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• v.110 no.4
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• pp.543-553
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• 2021

This research focused on the investigation of isoprene and terpene emissions from 30 major urban tree species. We conducted sampling using a specific dynamic enclosure system between August and September 2020. Seedlings less than three years old were enclosed in a chamber consisting of a 400 L transparent Tedlar bag. The air-flows from the chamber's outlet were sampled using Tenax-filled sorbent tubes in the presence of standard conditions (temperature: 30℃, PAR: 1,000 μmol/m²/sec). A thermal desorption GC/MS system was used to analyze 38 BVOC compounds (isoprene, monoterpene, sesquiterpene, oxygenated monoterpene, and sesquiterpene). Isoprene emitters included Phyllostachys bambusoides, Quercus serrata, Daphniphyllum macropodum, and Buxus Koreana. Monoterpene emitters included Pinus rigida, Acer pictum subsp. mono, Larix kaempfer, Magnolia denudata, Metasequoia glyptostroboides, Pinus koraiensis, Pinus densiflora, and Abies holophylla. The monoterpene emission profiles were dominated by α-pinene, myrcene, limonene, β-pinen, and sabinene, while caryophyllene and farnesene were the prominent sesquiterpenes. Predominant oxygenated monoterpene compounds were also discovered as pulegone, borneol, menthol, eucalyptol, and nerol, while caryophyllene oxide were the prominent oxygenated sesquiterpenes. Sesquiterpenes and oxygenated sesquiterpenes had relatively lower contributions for all species.

• Journal of Animal Reproduction and Biotechnology
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• v.35 no.4
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• pp.297-306
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• 2020

The aims of the present study were to confirm that regulation of the PA and environment via TGF-β regulation of sperm by Percoll-separated in porcine uterine epithelial cells. And, it was performed to identify the cytokines (TGF-β1, 2 and 3, TGF-β receptor1 and 2; interleukin, IL-6, IL-8) and PA-related genes (urokinase-PA, uPA; tissue-PA, tPA; PA inhibitor, PAI; uPA-receptor, uPAR) by spermatozoa. The experiment used porcine uterus epithelial cells (pUECs) and uterine tissue epithelial cells, Boar sperm were separated by discontinuous Percoll density gradient (45/90%), and tissues were co-incubated with spermatozoa, followed by real-time PCR. PA activity was measured of sperm by discontinuous Percoll density gradient (45/90%) for 24 hours. To measure viability and acrosome damage of sperm double stained propidium iodide (PI) and SYBR-14 or FITC-PNA were used. In results, binding ratio of Percoll-separated sperm was found no differences, but sperms isolated from 90% Percoll layer reduced PA activity (p < 0.05). when co-cultured sperm selected Percoll in porcine uterus tissues epithelial cells, 90% layer sperm increased TGF-β R1, contrastively tPA and PAI-1 in comparison with control (p < 0.05). 45% sperm was decreased the expression of uPA (p < 0.05). TGF-β decreased PA activity in the supernatant collected from pUECs (p < 0.05). Especially, The group including uPA, PAI-1 were induce sperm intact, while it was reduced in sperm damage when compared to control (p < 0.05). Also, there was no significant difference group of tPA and tPA+I in the dead sperm and acrosome damage compared to control. The expression of tPA and PAI showed a common response. Percoll-separated spermatozoa in 90% layer reduced tPA and IL-related gene mRNA expression. Thus, Percoll-sparated sperm in 90% layer show that it can suppress inflammation through increased expression of TGF-β and downregulation of PA and IL in epithelial cells compared to 45% layer Percoll.

• Journal of Korean Society of Forest Science
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• v.111 no.4
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• pp.490-501
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• 2022

In this study, the isoprene and terpene emissions from 32 major urban tree species were investigated. We conducted sampling using a dynamic enclosure system between June and July 2021. Seedlings aged < three years were enclosed in a chamber consisting of a 400 L transparent Tedlar bag. The air flow from the outlet of the chamber was sampled using Tenax-filled sorbent tubes under standard conditions (temperature: 30°C; PAR: 1,000 μmol/m²/sec). A thermal desorption gas chromatography/mass spectrometry system was used to analyze the following 38 biogenic volatile organic compounds: isoprene, monoterpenes, sesquiterpenes, oxygenated monoterpenes, and oxygenated sesquiterpenes. Isoprene emitters included Quercus mongolica, Salix koreensis, Robinia pseudoacacia, and Salix chaenomeloides. Monoterpene emitters included Pinus strobus, Cedrela sinensis, and Cercis chinensis. The monoterpene emission profiles were dominated by á-pinene, myrcene, camphene, and limonene. The predominant oxygenated monoterpene and oxygenated sesquiterpene were eucalyptol and caryophyllene oxide, respectively. For all species, the contributions of sesquiterpenes and oxygenated sesquiterpenes were relatively low.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2022.09a
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• pp.85-85
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• 2022

영농형 태양광발전은 태양의 일사량을 전기발전과 영농에 공유하는(solar-sharing) 방식이다. 본 연구는 신재생에너지의 활용의 극대화를 위하여 추적식 영농형 태양광발전시스템을 구축하고 시설하부에서 일정 기간 재배중인 작물의 하부 환경과 생육을 조사하여 영농형태양광 하부작물개발을 위한 기초자료를 확보하고자 하였다. 구축한 추적식 영농형 태양광발전시스템은 4열 6단의 24장 모듈(8m × 6m)을 가지며, 발전시설 중심축 기둥 간 중심간격 14m로 단일지주식 스크루 공법으로 순천대학교 부속농장 답작포(순천시 죽평리)에 설치하여 하부 환경과 하부작물의 생육을 조사하였다. 태양광발전시설 하부작물의 생육을 조사하기 위하여 순천 농협육묘장에서 벼(신동진)를 육묘하여 2022년 6월 16일 이앙하였다. 태양광발전시스템 하부 지역을 4방위 방향에 따라 강음영(중심축으로부터 1~3m), 중음영(5m), 약음영(7~9m) 구역으로 설정하여 생육을 조사한 결과, 방위에 따른 초장은 남쪽에서 음영간 차이가 상대적으로 낮게 나타났으며, 1번기 태양광 발전시설에 의하여 음영이 중첩된 2번기 시설의 동쪽에서 대조구 대비 초장이 상대적으로 낮은 경향을 나타내었다. 음영강도에 따른 초장은 대체로 강음영구에서 낮게 나타났으며, 약음영구로 갈수록 높게 나타났다. 엽수는 방위에 따라서, 그리고 음영의 강도에 따른 차이가 초장에 비하여 작게 나타났다. 출수기의 경우 방위별로는 남쪽에서 음영별 차이가 작게 나타났으며, 음영강도에 따라서 차이를 보였다. 또한 태양광시설 하부에 데이터수집장치(Model 1650, Spctrum Technonogies, USA)를 설치하여 음영에 따른 토양전도도, 토양함수량, 토양온도, par light 등 생육환경을 조사, 비교하였다.

• Korean Journal of Environment and Ecology
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• v.17 no.2
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• pp.144-152
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• 2003

The purpose of this study was to investigate the effects of lime fertilization and light intensity on beech regeneration strategy in a mature beech forest(ca. 150 years old) gaps, which was located in the soiling areas Germany, where the soil is an acid brown earth. For the experiment canopy gaps of 30m diameter were cut, and one canopy gap remained as untreated while the other was limed(3t dolomite h $a^{-1}$). Soil chemistry, soil water potential, light intensity(PAR), ground vegetation, beech seedlings and mycorrhiza were investigated along transect from the stands into gaps to the north. The results of this study are followings: 1) In the limed gap, soil chemistry was greatly improved and a vigorous herb layer developed as compared to the unlimed gap. 2) Development and growth of the beech seedlings in the unlimed gap were generally deficient. Specially, developments of lateral roots were very lack. 3) Through the lime fertilization, the minerals contents of beech leaves such as calcium(Ca) and magnesium(Mg) were increased, while manganese(Mn) was decreased. There was antagonism between potassium(K) and calcium(Ca) or magnesium(Mg). 4) The relative mycorrhiza frequency(RM) in beech seedlings of the southern edge was very higher than that of the middle part, and through the lime fertilization extension of the species Laccaria amethystina appeared clearly. The results of this study showed that lime fertilize in forest, where the soil is acid, will be necessary..

• Journal of the Korean Institute of Landscape Architecture
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• v.36 no.4
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• pp.15-26
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• 2008

The purpose of this study is to attempt to interpret golf courses as event-generating spaces with consideration given to the time factor. Through a golf game, a variety of events such as the tee shot, second shot, putt, and hole out are generated. These events have been connected to a series of events after hole out such as birdie, par, bogey and so on. The series of events do not always occur in the same way. They reveal unexpected changes over time. These unexpected changes cause changes in the spatial characteristics and offer unforgettable memories for golfers. Gilles Deleuze mentioned the spatial characteristics as striated space and smooth space. Striated space can be defined as sedentary space. It is distant vision-optical space that has dimensional, metric and centered characteristics, whereas smooth space is defined as nomadic, close vision-haptic space that has directional and acentered characteristics. This study focused on the analysis of spatial characteristics according to striated space and smooth space. Golf courses generally show the characteristics of striated space before beginning the game. As soon as the game begins, however, the golf courses are converted into an event-generating space. The characteristics of striated space are transformed into smooth space, a nomadic space that amplifies the dynamic, changeable, de-scaled and non-metric system. Through the whole game, this transformation is dramatically repeated. On the other hand, the golfer, the subject of the game, senses the phenomenological experience in the process of orientation, center, definition, and domestication.

• Journal of Korean Neurosurgical Society
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• v.29 no.8
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• pp.985-994
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• 2000

Objective : There are some advantages of trigeminal evoked potential(TEP) recording compared to other somatosensory evoked potential(SSEP) recordings. The trigeminal sensory pathway has a pure sensory nerve branch, a broader receptive field in cerebral cortex, and a shorter pathway. Despite these advantages, there is little agreement as to what constitutes a normal response and what wave forms truly characterize the intraoperative TEP. This study presents the normative data of TEP recorded on the epidural surface of the rat with a platinum ball electrode. Materials & Methods : Under general anesthesia with urethane, the adult Sprague-Dawley male rats(300-350g) were given electrical stimulation with two stainless steel electrodes which were inserted into the subcutaneous layer of the area around whiskers. A reference electrode was positioned in the temporalis muscle ipsilateral to the recording site. Results : TEPs were recorded in the Par I area of somatosensory cortex and recorded most apparently on the point of 2mm posterior from the bregma and 6mm lateral from the midline. The typical wave form consisted of 5 peaks (N1-P1-N2-P2-N3 according to emerging order, upward negativity). Each latency to corresponding peaks was not influenced by the different intensities of stimulation, especially from 1 to 5mA. Average latencies of 5 peaks were in the following order ; 7.7, 11.1, 15, 22.3, 29.4ms. There was also no significant difference between latencies before and after administration of muscle relaxant(pancuronium). For the electrophysiological localization of recorded waves, the action potential of a single unit was recorded with glass microelectrode(filled with 2M NaCl, $3-5M{\Omega}$) in the thalamus of rat. A sharp wave was recorded in the VPM nucleus, in which the latency was shorter than that of N1. This suggests that all 5 peaks were generated by neural activities in the suprathalamic pathway. Conclusion : In terms of recording near-field potentials, our data also suggests that TEP in the rat may be superior to other SSEPs. In overall, these results may afford normative data for the studies of supratentorial lesions such as hydrocephalus or cerebral ischemia which can have an influence on near-field potentials.

• Korean Journal of Weed Science
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• v.16 no.2
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• pp.160-170
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• 1996

Several physiological responses were investigated in plants treated with TOPE as a preliminary step to know its action site. Unlike photo-dependent diphenylethers, herbicidal activity of TOPE appeared slowly and its typical symptoms were both burning of leaf blades and abnormal division of meristem in grasses, Similarly, both leakage of cell electrolytes and the curling of cotyledon margin were also shown in cucumber(Cucumis sativus L.). Biosynthesis of chlorophyll in etiolated cucumber cotyledon was not inhibited directly by treatment of TOPE at low light intensity(5.5${\mu}$ mol $m^{-2}s^{-1}$ PAR) and protoporphyrin IX was not also accumulated. The contents of phytoene, phytofluene and ${\beta}$-carotene were abnormaly increased. Photosynthesis was inhibited only at high concentration. Mitochondrial respiration was inhibited at high concentration but rather increased significantly at 10${\mu}$M of TOPE. However, respiration inhibitors did not alleviate the two symptoms of TOPE in cucumber cotyledon. In the same experiments, using inhibitors of protein or nucleic acid biosynthesis, only one of the two symptoms was alleviated by chloramphenicol and cycloheximide. In contrast, both symptoms were alleviated by actinomycin-D and hydroxyurea, suggesting that nucleic acid metabolism might be preferentially related to the mode of action of TOPE. DNA, RNA and protein contents were accumulated in both cucumber cotyledon and rice (Oryza sativa L.) routs treated with TOPE, and the DNA of them was increased at first. Thus, it is conjectured that TOPE increase nucleic acid metabolism directly or indirectly, and then disturb various metabolic pathways causing abnormal physiological and morphological effects followed by final death.

• Journal of the Korean Geographical Society
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• v.43 no.4
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• pp.561-579
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• 2008

The population concentration in Seoul has caused the chronic housing shortage. Accordingly the new towns in the Capital region were developed to alleviate overcrowding conditions in Seoul. The purpose of this study is to analyze changes of migration and commuting patterns according to new town development in the Capital region for the period of $1995{\sim}2005$. Further this study examines the changes of self-sufficiency level for new towns using jobs to housing ratio. During the last 10 years, the migration pattern in the Capital region has been pretty much followed the new town development. Such a migration pattern has influenced the commuting patterns, expanding the Seoul Metropolitan Area into northeastern par of the Capital region. The result reveals that self-sufficiency levels of new towns have become higher over the period of $1995{\sim}2005$, indicating that new towns are gradually strengthening their economic functions and have potential to become new business centers in the future. Therefore, the policy focusing on the increase of the job-housing balance ratio and self-sufficient level in new towns will be a desirable policy alternative to solve the transportation problems in the Capital region.