• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제47권6호
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• pp.438-444
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• 2021

Objectives: Loss of the interdental papilla is multi-factorial and creates a multitude of problems. Autogenous connective tissue/biomaterial-based regeneration has been attempted for decades to reconstitute the black space created due to the loss of papilla. The aim of this present study was to regenerate papillary recession defects using an amnion-chorion membrane (ACM) allograft and to evaluate the clinical outcome up to six months postoperatively. Materials and Methods: Twenty patients with 25 Nordland and Tarnow's Class I/II interdental papillary recession defects were treated with ACM and coronal advancement of the gingivo-papillary unit via a semilunar incision on the labial aspect followed by a sulcular incision in the area of interest. A photographic image analysis was carried out using the GNU Image Manipulation software program from the baseline to three and six months postoperatively. The black triangle height (BTH) and the black triangle width (BTW) were calculated using the pixel size and were then converted into millimeters. The mean and standard deviation values were determined at baseline and then again at three and six months postoperatively. The probability values (P<0.05 and P≤0.01) were considered statistically significant and highly significant, respectively. An analysis of variance and post hoc Bonferroni test were carried out to compare the mean values. Results: Our evaluation of the BTH and BTW showed a statistically and highly significant difference from the baseline until both three and six months postoperatively (P=0.01). A post hoc Bonferroni test disclosed a statistically significant variance from the baseline until three and six months postoperatively (P<0.05) and a non-significant difference from three to six months after the procedure (P≥0.05). Conclusion: An ACM allograft in conjunction with a coronally advanced flap could be a suitable minimally invasive alternative for papillary regeneration.

• 대한화장품학회지
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• 제48권4호
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• pp.385-392
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• 2022

본 연구에서는 녹용에 함유한 단백질인 케라틴을 Fervidobacterium islandicum AW-1로 고온 혐기 발효를 통하여 케라틴 펩타이드를 생산하고, 인체 모발 관련한 인자를 확인하여, 화장품 원료로서 케라틴 펩타이드의 가능성을 확인하였다. 모유두 세포주에서 녹용 발효 케라틴 펩타이드를 농도에 따라 세포 독성 및 증식을 확인한 결과, 세포 독성은 나타나지 않았고, 세포 증식 효과를 보였다. 인체 모유두 세포에 대하여 녹용 발효 케라틴 펩타이드에 따른 성장인자의 증가를 확인하여 모발에 영향을 준다고 판단하였다. 이러한 녹용 발효 케라틴 펩타이드를 함유한 화장품을 제조하여 피부 안전성 및 탈모 증상 완화 인체 효력 시험을 수행한 결과, 사용 12 주 후에 제품 사용 전 대비 전체 모발 수의 증가 및 대조군과 비교시 전체 모발 개수의 차이가 통계적으로 유의하게 나타났다. 이를 통해 녹용 발효 케라틴 펩타이드는 기능성 화장품 원료뿐만 아니라 건강 기능 식품 소재로의 가능성을 확인하였다.

• 한국축산식품학회지
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• 제44권1호
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• pp.204-215
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• 2024

This study was designed to examine the effect of Lactilactobacillus curvatus LB-P9 on hair regeneration. The treatment of LB-P9 conditioned medium increased the proliferation of both hair follicle dermal papilla cells and hair germinal matrix cells (hGMCs). Moreover, the expression levels of hair growth factors such as vascular endothelial growth factor (VEGF) and fibroblast growth factor 7 were significantly elevated in hGMCs co-cultured with LB-P9. After time-synchronized depilation, mice were orally administered with either 4×10⁷ colony forming unit (CFU) of LB-P9 (low dose) or 4×10⁸ CFU of LB-P9 (high dose), once daily for 4 weeks. Compared with the vehicle (phosphate-buffered saline)-administrated group, the LB-P9-treated groups exhibited accelerated hair regrowth rate and enhanced hair thickness in a dose-dependent manner. Supporting this observation, both hair follicle numbers and the dermal thickness in skin tissues of the LB-P9-treated groups were increased, compared to those of the vehicle-treated group. These results might be explained by the increased level of β-catenin and number of hair follicle stem cells (CD34⁺ CD49f⁺ cells) in the skin tissues of mice administered with LB-P9, compared to the vehicle-treated mice. Also, increased serum levels of hair growth factors such as VEGF and insulin-like growth factor-1, and superoxide dismutase were found in the LB-P9-treated groups, compared to those of the vehicle-treated group. Taken together, these results might demonstrate that the oral administration of LB-P9 promotes hair regeneration by the enhancement of dermal papilla proliferation through the stimulation of hair growth factor production.

• 대한본초학회지
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• 제39권1호
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• pp.23-29
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• 2024

Objectives : Polyporus umbellatus is a medicinal mushroom that has been used for over thousands years in Chinese medicine as a powerful diuretic to relieve fluid retention and edema. Dermal papilla is located at the bottom of the hair follicle and connected to the blood vessels where it gets the nutrients and oxygen to nurture hair follicle. This study examined the mechanism through which the ethanol extract of Polyporus umbellatus (EPU) promoted the proliferation of human dermal papilla cells (HHDPCs). Methods : To estimate the proliferative effects of EPU on HHDPCs, cell viability was estimated by thiazolyl blue tetrazolium bromide (MTT) assay. Western blotting was used to investgate the activation of ERK, phosphoinositide 3-kinase (PI3K)/Akt, β-catenin, GSK-3β and heme oxygenase-1 (HO-1). Cells were treated with inhibitors of ERK and Akt prior to EPU treatment. Results : EPU promoted the proliferation of HHDPCs and the phosphorylation of ERK and Akt in dose dependent manner. However, the proliferative effect of EPU on HHDPCs was inhibited by pre-treatment of ERK inhibitor (PD98059) and Akt inhibitor (LY294002). Furthermore, EPU respectively stimulated the protein expression of β-catenin and phosphorylated GSK-3β. EPU significantly increased the protein expression levels of proliferation and cytoprotection related genes such as Bcl-2, SIRT-1, and HO-1 in cells. Conclusion : This results suggest that EPU promoted the proliferation of HHDPCs via activating PI3K/Akt and Wnt/β-catenin signaling pathway in HHDPCs.

• Journal of Microbiology and Biotechnology
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• 제33권11호
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• pp.1467-1474
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• 2023

Due to the continuous increase in patients with androgenetic alopecia (AGA) and psychological disorders such as depression and anxiety, the demand for hair loss treatment and effective hair growth materials has increased. Terminalia bellirica (Gaertn.) Roxb. (TBE) reportedly exerts anti-inflammatory, hepatoprotective, and antidiabetic effects, among others, but its effects on testosterone (TS)-inhibited hair growth remains unclear. In this study, we evaluated the effects of TBE on TS-induced hair growth regression in human follicle dermal papilla cells (HFDPCs) and C57BL/6 mice. Oral administration of TBE increased TS-induced hair growth retardation. Interestingly, effects were greater when compared with finasteride, a commercial hair loss treatment product. Histological analyses revealed that oral TBE administration increased hair follicles in the dorsal skin of C57BL/6 mice. Additionally, western blotting and immunofluorescence showed that oral TBE administration recovered the TS-induced inhibition of cyclin D1, proliferating cell nuclear antigen (PCNA), and Ki67 expression in vivo. Using in vitro proliferation assays, TBE promoted HFDPC growth, which was suppressed by TS treatment. Thus, TBE may be a promising nutraceutical for hair health as it promoted hair growth in AGA-like in vitro and in vivo models.

• Journal of Microbiology and Biotechnology
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• 제34권9호
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• pp.1810-1818
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• 2024

Autophagy is essential for regulating hair growth. Accordingly, we developed autophagy activator ICP5249 (pentasodium tetracarboxymethyl palmitoyl dipeptide) and investigated its potential role in hair growth. We evaluated its effect on hair growth using in vitro human dermal papilla cells (hDPCs) culture model, human hair follicles (hHFs) organ culture model, and telogenic mouse model. ICP5249 increased hDPCs proliferation and alkaline phosphatase (ALP) expression. It also increased microtubule-associated protein (MAP) light chain 3-II (LC3-II) expression and AMP-activated protein kinase α (AMPKα) and unc-51-like kinase 1 (ULK1) phosphorylation in hDPCs. ICP5249 extended the length of hHFs and increased LC3-II please revised from LC3 II to LC3-II in all manuscript expression. Consistently, ICP5249 also significantly increased hair growth area, dermis thickness, and anagen and telogen ratio in telogenic mice. Furthermore, it upregulated Ki-67 and LC3-II expression and AMPKα phosphorylation on the mice's dorsal skin. To investigate whether AMPK regulates ICP5249-induced hair growth, following treatment with the compound C, AMPK inhibitor, the activity of ICP5249 was evaluated. The effects of ICP5249 on hair growth were assessed following pretreatment with the AMPK inhibitor compound C. The results showed that compound C suppressed ICP5249-mediated proliferation and hair inductivity in hDPCs. Additionally, compound C inhibited ICP5249-mediated hair growth area, dermis thickness, anagen and telogen ration, and LC3-II expression in mice, suggesting that ICP5249 promotes hair growth by modulating autophagy, with AMPKα playing a regulatory role in this process. Taken together, we demonstrate that ICP5249 has the potential as an ingredient for improving hair growth.

• 한국식품영양과학회지
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• 제46권6호
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• pp.671-680
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• 2017

본 연구 결과 검은콩 물 에탄올 추출물과 Lactobacillus rhamnosus GG(LGG)와 Bifidobacterium animals subsp. lactis BB-12(BB-12)를 이용하여 발효시킨 발효검은콩 물 에탄올 추출물의 발효 전과 후의 성분변화를 분석하고, 검은콩과 검은콩 발효 추출물이 모유두 세포 성장에 미치는 효과를 알아보기 위해 primary 인간 모유두 세포(HFDPC)에 검은콩 추출물(BWE, BEE)과 발효검은콩 추출물(BWE-F, BEE-F)을 다양한 농도로 처리하여 세포독성을 확인하였으며, 이를 바탕으로 적정 처치 농도를 결정하여 모발 성장촉진(VEGF와 KGF/FGF7)과 억제($TGF-{\beta}1$과 AR)에 관여하는 유전자 발현을 mRNA 수준에서 확인하였다. 나아가 검은콩 추출물이 HFDPC의 생존 촉진에 미치는 영향을 Akt와 Erk의 인산화 활성을 통해 비교 분석하였다. LGG와 BB-12를 이용하여 발효시킨 검은콩은 발효가 진행됨에 따라 pH, 총폴리페놀, 총당 환원당의 함량이 감소하였으며, 검은콩 4종 추출물(BWE, BEE, BWE-F, BEE-F) 중 BWE, BEE, BWE-F가 VEGF의 mRNA 발현을 증가시키고, 모든 처리군에서 KGF/FGF7의 mRNA 발현을 유의적으로 증가시킴을 확인할 수 있었다. 나아가 BWE, BEE, BWE-F가 Erk의 활성을 증가시켰음을 확인할 수 있었다. 따라서 검은콩 물 추출물과 검은콩 에탄올 추출물, 그리고 발효검은콩 물 추출물이 인간 모유두 세포의 세포성장에 모발 성장 촉진인자의 활성과 Erk의 활성화 등을 통한 기전으로 긍정적인 영향을 미침으로써 모발 성장 및 모발 건강을 위한 기능성원료로서의 가능성을 확인할 수 있었으며, 유산균 2종(LGG, BB-12)을 이용한 발효검은콩이 검은콩과 비교하여 모발 성장 촉진 관련 단백질 발현에서는 유의적인 우월성을 가지지는 않음을 확인하였다. 추후 다른 유산균 균 총을 이용한 발효검은콩의 연구와 더불어 보다 정밀한 발효를 통한 검은콩 추출물의 성분과 조성의 변화를 바탕으로 한 모발 성장 및 모주기 관련 연구가 이루어져야 할 것이라 생각된다.

• 대한치과보철학회지
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• 제53권4호
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• pp.310-317
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• 2015

목적: 한국 성인 유치악자를 대상으로 치아와 안모 사이의 크기 및 형태적인 상관관계, 구강 내 해부학적 기준점들과 치아배치 양상 사이의 관계를 조사하여 총의치 제작 시 무치악 환자 치료에 사용되는 기준점들의 효용성을 평가하고자 하였다. 대상 및 방법: 서울대학교 치의학 대학원생을 대상으로 상악 6전치가 정상적으로 배열되어 있는 성인 남성 270명 여성 280명을 선정하여 연구를 수행하였다. 악안면부의 해부학적 구조를 크게 변화시킬 수 있는 외과적 치료를 받은 경우, 교정치료를 받은 경우, 선천적으로 상악 전치부 치아 형태 및 수에 이상이 있는 경우, 상악 전치부 치관의 변형을 초래할 수 있는 수복치료를 받은 경우는 연구대상에서 제외하였다. 연구항목은 안모의 외형, 협골궁 폭경, 안모의 길이, 상악중절치의 형태 및 장경, 상악6전치의 치관 폭경, 견치간 거리, 절치유두 중심에서 중절치 순면까지의 거리, 절치유두 중심에서 양견치 첨단을 지나는 선까지의 수직거리이었다. 이를 바탕으로 안면계측항목과 모형계측항목간의 비율 및 상관관계를 통계적으로 분석하였다. 결과: 본 연구의 한계 내에서 다음과 같은 결과를 얻었다. 1) 안모의 외형과 상악 중절치의 형태가 일치하는 비율은 남성 55.56%, 여성 46.43%, 전체 50.91%이었다. 1) 협골궁 폭경과 상악중절치 폭경, 상악6전치 연합폭경의 비율은 각각 16.8 : 1, 3.0 : 1이며 통계적으로 양의 상관관계가 있었다. 2) 상모학상 안모의 길이(Trichion-Gnathion)와 상악중절치 치관 장경, 코뿌리점-턱끝융기점간 거리(Nasion-Gnathion)와 상악중절치 장경과의 비율은 각각 19.7 : 1, 11.6 : 1이었고 남성에서는 코뿌리점-턱끝융기점간 거리가, 여성에서는 안모의 길이가 더 큰 상관관계가 있었다. 3) 절치유두 중심과 양 견치첨단을 연결하는 선 사이의 수직거리는 $1.33{\pm}1.08mm$이었다. 4) 절치유두 중심에서 중절치 순면까지의 거리는 $9.23{\pm}1.20mm$이었다. 결론: 총의치 제작에 사용되는 기준점들은 한국 성인 유치악자의 경우에도 통계적으로 효용성을 가지며, 중요한 해부학적 지표가 임상적으로 의미를 가진다고 할 수 있다. 그러나 통계적으로 수치에서 차이를 보이고 개별적인 편차를 가지므로 인공치아의 선택이나 배열 후 환자의 동의를 받는 과정이 필수적이라고 할 수 있다.

• Animal cells and systems
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• 제6권3호
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• pp.195-199
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• 2002

Four new species of the genus Amynthas are described from Korea： Amynthas mujuensis sp. nov., Amynthas assimilis sp. nov., Amynthas moakensis sp. nov., and Amynthas sangumburi sp. nov. The intestinal caeca are of simple type and genital markings lacking in all four species. Amynthas mujuensis sp. nov. has male field with large oval papillae, each papilla with longitudinal seminal groove； paired spermathecal pores present in 6/7, 7/8 dorsally. Amynthas assimilis sp. nov. has mare field composed of paired symmetrical pear-shaped pads with broad ends laterally, axes of symmetry diagonally oriented towards posterior； spermathecal pores in 5/6, 6/7. Amynthas moakensis sp. nov. has male pores on small porophores surrounded by swollen concentric furrows, with the spermathecal pores in 6/7, 7/8, 8/9, ventrally. Amynthas sangumburi sp. nov. has male pores centered on 0.8 mm circular flat porophores, with the spermathecal pores in 5/6, 6/7, 7/8, 8/9, laterally.

• Journal of Oral Medicine and Pain
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• 제23권4호
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• pp.327-341
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• 1998

Aims : The purpose of this study was to investigate whether there are any changes in taste sensitivity with advancing age and to see if smoking or oral hygiene can affect the taste sensitivity. Method : Nine hundred and thirty four subjects(458 male and 476 females) were included for the study and they were categorized into 4 age groups( under 20, 20 to 39, 40 to 59, and over 60 age group ). The electrical taste thresholds were measured using an electrogustometer for the 4 different sites in the oral cavity, I.e., tongue tip, tougue alteral, circumvallate papilla, and soft palate. Results : The elctrical taste thresholds were significantly incresed with advancing age in both gender, but the pattern of change is moere abrupt in female after 40. There were not significant differences in electrical taste threshold between smoking and non-smoking people. Taste thresholds were significantly lower in the groups with higher frequency of daily toothbrushing than the groups with lower frequency Conclusion : The electrical taste threshold is increased with aging. It is not influenced by smoking but by toothbrushing.