The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Proliferative Activity of Polyporus umbellatus Extract from Mushrooms via the PI3K/Akt and Wnt/β-catenine signaling in HHDPCs

사람 모유두세포에서 PI3K/Akt와 Wnt/β-catenine 신호전달을 경유한 저령추출물의 세포증식 효과

  • Lea-Minju Kang (Department of the Herbal Resources, Professional Graduate School of Korean Medicine, Wonkwang University) ;
  • Suk-Jong Kang (Department of Convergence Technology for Food Industry, Graduate School of Wonkwang University) ;
  • Yeun-Ja Mun (Department of the Herbal Resources, Professional Graduate School of Korean Medicine, Wonkwang University)
  • 강리아민주 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 강석종 (원광대학교 대학원 식품산업융복합학과) ;
  • 문연자 (원광대학교 한의학전문대학원 한약자원개발학과)
  • Received : 2023.12.12
  • Accepted : 2024.01.25
  • Published : 2024.01.30
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Abstract

Objectives : Polyporus umbellatus is a medicinal mushroom that has been used for over thousands years in Chinese medicine as a powerful diuretic to relieve fluid retention and edema. Dermal papilla is located at the bottom of the hair follicle and connected to the blood vessels where it gets the nutrients and oxygen to nurture hair follicle. This study examined the mechanism through which the ethanol extract of Polyporus umbellatus (EPU) promoted the proliferation of human dermal papilla cells (HHDPCs). Methods : To estimate the proliferative effects of EPU on HHDPCs, cell viability was estimated by thiazolyl blue tetrazolium bromide (MTT) assay. Western blotting was used to investgate the activation of ERK, phosphoinositide 3-kinase (PI3K)/Akt, β-catenin, GSK-3β and heme oxygenase-1 (HO-1). Cells were treated with inhibitors of ERK and Akt prior to EPU treatment. Results : EPU promoted the proliferation of HHDPCs and the phosphorylation of ERK and Akt in dose dependent manner. However, the proliferative effect of EPU on HHDPCs was inhibited by pre-treatment of ERK inhibitor (PD98059) and Akt inhibitor (LY294002). Furthermore, EPU respectively stimulated the protein expression of β-catenin and phosphorylated GSK-3β. EPU significantly increased the protein expression levels of proliferation and cytoprotection related genes such as Bcl-2, SIRT-1, and HO-1 in cells. Conclusion : This results suggest that EPU promoted the proliferation of HHDPCs via activating PI3K/Akt and Wnt/β-catenin signaling pathway in HHDPCs.

Keywords

Acknowledgement

본 연구는 2022년도 원광대학교 교비지원에 의해 수행되었습니다.

References

  1. Hedgepeth CM, Conrad LJ, Zhang J, Huang HC, Lee VM, Klein PS. Activation of the Wnt Signaling Pathway: A Molecular Mechanism for Lithium Action. Developmental Biology. 1997;185(1):82-91. https://doi.org/10.1006/dbio.1997.8552
  2. Matsuzaki T, Yoshizato K. Role of Hair Papilla Cells on Induction and Regeneration Processes of Hair Follicles. Wound Repair Regeneration. 1998;6(6):524-30. https://doi.org/10.1046/j.1524-475X.1998.60605.x
  3. Yonhapnews (2011). "Alopeica is genetic?...40% Shows It's Non-genetic". 2011. 08. 30. Released.
  4. Health Insurance Review and Assessment Service. 'Male Pattern Alopecia' Is Constantly Rising 4.8% Per Year. 2014. 05. 02. Press Release.
  5. Buhl AE, Waldon DJ, Miller BF, Brunden MN. Differences in Activity of Minoxidil and Cyclosporin A on Hair Growth in Nude and Normal Mice. Comparisons of in Vivo and in Vitro Studies. Laboratory Investigation. 1990;62(1):104-7.
  6. Kaufman KD, Olsen EA, Whiting D, Savin R, Devillez R, Bergfeld W. Finasteride in the Treatment of Man With Androgenetic Alopecia. Journal of Cosmetic Dermatology. 1998; 39(1):578-89.
  7. Hagemann T, Schlutter-Bohmer B, Allam JP, Bieber T, Novak N. Positive Lymphocyte Transformation Test in a Patient With Allergic Contact Dermatitis of the Scalp After Short-term Use of Topical Minoxidil Solution. Contact Dermatitis. 2005 ; 53(1): 53-55. https://doi.org/10.1111/j.0105-1873.2005.00456b.x
  8. Chung SM, Wang IC, Yang JL. Roles of JNK, P38 and ERK Mitogen-Activated Protein Kinases in the Growth Inhibition and Apoptosis Induced by Cadmium. Carcinogenesis. 2000;21(7):1423-32. https://doi.org/10.1093/carcin/21.7.1423
  9. Greco V, Chen T, Rendl M, Schober M, Pasolli HA, Stokes N, et al. A Two-Step Mechanism for Stem Cell Activation During Hair Regeneration. Cell Stem Cell. 2009;4(2):155-69.
  10. Inaoka Y, Shakuya A, Fukazawa H, Ishida H, Nukaya H, Tsuji K, et al. Studies on Active Substances in Herbs Used for Hair Treatment. I. Effects of Herb Extracts on Hair Growth and Isolation of an Active Substance From Polyporus umbellatus F. Chemical and Pharmaceutical bulletin. 1994;42(3):530-4. https://doi.org/10.1248/cpb.42.530
  11. Ishida H, Inaoka Y, Shibatani J, Fukushima M, Tsuji K. Studies of the Active Substances in Herbs Used for Hair Treatment. II. Isolation of Hair Regrowth Substances, Acetosyringone and Polyporusterone A and B, From Polyporus umbellatus Fries. Biological and Pharmaceutical Bulletin. 1999;22(11):1189-92. https://doi.org/10.1248/bpb.22.1189
  12. Lavoie JN, L'Allemain G, Brunet A, Muller R, Pouyssegur J. Cyclin D1 Expression Is Regulated Positively by the p42/p44MAPK and Negatively by the p38/HOGMAPK Pathway. Journal of Biological Chemistry. 1996;271(34):20608-16.
  13. Leiros GJ, Attorresi AI, Balana ME. Hair Follicle Stem Cell Differentiation Is Inhibited Through Cross-talk Between Wnt/beta-catenin and Androgen Signaling in Dermal Papilla Cells From Patients With Androgenetic Alopecia. The British Journal of Dermatology. 2012;166(5):1035-42. https://doi.org/10.1111/j.1365-2133.2012.10856.x
  14. Guo H, Yang K, Deng F, Ye J, Xing Y, Li Y, et al. Wnt3a Promotes Melanin synthesis of Mouse Hair Follicle Melanocytes. Biochemical and Biophysical Research Communications. 2012;420(4):799-804. https://doi.org/10.1016/j.bbrc.2012.03.077
  15. Hasegawa K, Yoshikawa K. Necdin Regulates p53 Acetylation via Sirtuin1 to Modulate DNA Damage Response in Cortical Neurons. The Journal of Neuroscience. 2008;28(35):8772-84. https://doi.org/10.1523/JNEUROSCI.3052-08.2008
  16. Hasegawa K, Wakino S, Yoshioka K, Tatematsu S, Hara Y, Minakuchi H, et al. Sirt1 Protects Against Oxidative Stress-Induced Renal Tubular Cell Apoptosis by the Bidirectional Regulation of Catalase Expression. Biochemical and Biophysical Research Communications. 2008;372(1):51-66. https://doi.org/10.1016/j.bbrc.2008.04.176
  17. Alka M, Joshi V, Amitesh K, Ritu V, Anu TS, Manu J, et al. In Vitro Hair Growth Promoting Effects of Naringenin and Hesperetin on Human Dermal Papilla Cells and Keratinocytes. American Journal of Dermatology and Venereology. 2017;6(3):51-7.
  18. Naito A, Midorikawa T, Yoshino T, Ohdera M. Lipid Peroxides Induce Early Onset of Catagen Phase in Murine Hair Cycles. International Journal of Molecular Medicine. 2008; 22(6):725-9.
  19. Jo S, Choi J, Yoon SJ, Yoon SY, Lee JY, Park WS, et al. Valproic acid Promotes Human Hair Growth in Vivo Culture Model. Journal of Dermatological Science. 2013;72(1):16-24. https://doi.org/10.1016/j.jdermsci.2013.05.007
  20. Park SA, Ko KS, In MH, Mun YJ, Woo WH. Effect of Puerariae radix Ethanol Extract on the Proliferation of Human Dermal Papilla Cells. Korean Journal of Oriental Physiology and Pathology. 2017;31(3):167-72. https://doi.org/10.15188/kjopp.2017.06.31.3.167
  21. Chong ZZ, Shang YC, Wang S, Maiese K. SIRT1: New Avenues of Discovery for Disorders of Oxidative Stress. Expert Opinion on Therapeutic Targets. 2012;16(2):167-78. https://doi.org/10.1517/14728222.2012.648926
  22. Park J, Hao L, Jung ES, Yun SK, Kim HU, Lee Y, et al. SIRT1 Activation Ameliorates Inflammation in The Outher Root Sheat Cells of Hair Follicles. Korean Journal of Dermatology. 2018;70(2):442-3.
  23. Wojas-Pelc A, Marcinkiewicz J. What Is a Role of Haeme Oxygenase-1 in Psoriasis? Current Concepts of Pathogenesis. International Journal of Experimental Pathology. 2007;88(2):95-102. https://doi.org/10.1111/j.1365-2613.2006.00505.x