• Food Science and Biotechnology
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• v.16 no.3
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• pp.349-354
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• 2007

The objectives of this study were to systemically identify phenolic compounds in roasted wild ginseng (Panax ginseng C.A. Meyer) leaves and investigate their radical scavenging activities. Seven phenolic compounds were identified by NMR (H, C, COSY, HMQC, HMBC) and mass (EI-MS, FAB-MS) analyses: 5-caffeoylquinic acid, kaempferol, quercetin, 3,4-dihydroxy-benzoic acid, 4-hydroxy-benzoic acid, 3-(3,4-dihydroxyphenyl)-2-propenoic acid, and 3-(4-hydroxy-3-methoxyphenyl)-2-propenoic acid. Their concentrations ranged from 0.4 (3,4-dihydroxy-benzoic acid) to 7.5 mg (kaempferol) per 100 g of roasted leaves. Among these compounds, 5-caffeoylquinic acid, kaempferol, and quercetin were found exclusively in the leaf portions of the ginseng plants. When their antioxidant activities were measured by DPPH and superoxide anion radical scavenging activity, quercetin, and kaempferol were most effective.

• Korean Journal of Medicinal Crop Science
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• v.23 no.4
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• pp.284-291
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• 2015

This study was performed to investigate the effects of enhanced light transmission on plant growth, photosynthetic ability, and disease tolerance to leaf blight, anthracnose in ginseng (Panax ginseng C. A. Meyer, Araliacease family) during the early growth stage (April to June). The photosynthetic ratio, stomatal conductance, and stem diameter of plants grown under a shade net with 15% light transmission rate showed an increasing trend compared to the control plants (5% light transmission rate) although the growth of the aerial parts were not influenced significantly. Plant height, stem length, and leaf length of treated plants were not significantly different from those of the control plants. Root parameters, such as root length, diameter, and weight of treated plants increased significantly compared to the control. Yield performance ($187.4kg{\cdot}10a^{-1}$) of treated plants was 55.5% higher than that of the control ($150.4kg{\cdot}10a^{-1}$). Additionally, disease severity scores of treated plants were lower than those of the control plants, revealing higher survival rates. To retain high yield potential and enhance the level of disease tolerance in ginseng, we suggest the increase of light transmission rate during the early growth stage.

• Journal of Ginseng Research
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• v.11 no.2
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• pp.118-122
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• 1987

This study was carried out to investigate the effective components, especially saponins, in aerial parts of Panax ginseng. The contents of methanol and ethanol extracts in ginseng leaf were 35.9% and 27.3%, much higher than 15.4% and 8.37% in ginseng root and 21.7% and 16.3% in ginseng stem. And ginseng stem showed as high content of crude fiber as 39.2% which is very high compared with other two parts of ginseng. The contents of total crude saponin were 4.78%, 2.38% and 19.58% in ginseng root, stem and leaf, respectively. In ginseng leaf seven root ginseno-sides-ginsenoside-Rgl(3.32%), -Re(3.24%), -Rd(2.32 %), -Rc(0.65%), -Rb2(0.92%), -Rbl(0.29%), and -Rf(0.11%)-were analyzed by HPLC, Seven gisneno- sides-ginsenoside-Rgl(0.28%), -Re(0.3%), -Rd(0.05%), -Rf(0.01%), -Rc(trace), -Rb2(trace) and -Rbl(trace)-were detected in ginseng stem. Ginseng leaf contained high percentage of saponin and especially of ginsenoside-Rgl, -Re and -Rd. Therefore, ginseng leaf was good resources for ginsenoside-Rgl, -Re and -Rd.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.27 no.1
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• pp.72-77
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• 1982

To develop a new propagation method of Korean ginseng (Panax ginseng C.A. Meyer) by cutting, stem cuttings with leaflets obtained from ginseng seedlings were planted in rooting media treated with three levels of NAA concentration. NAA solution of 1.0 ppm was the most effective for the rooting of ginseng stem cuttings as well as for root growth after initiation of the roots from cuttings. A mixture of sand and leaf compost as a rooting medium for ginseng stem cuttings showed the best results in rooting percentage of cuttings and root growth after rooting. An acril film cap covering was very effective to prevent water loss by transpiration from the leaflets of stem cuttings and for rooting and root growth after rooting. Cuttings of leaf petiole with 5 leaflets from 2 year old ginseng plants showed good rooting as well as root growth after rooting in a mixture of sand and leaf compost treated with 1.0 ppm NAA.

• Journal of Ginseng Research
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• v.34 no.3
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• pp.227-236
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• 2010

The objective of this study was to evaluate the potential use of ginseng leaf as a cosmetic material. In this research, we employed enzymatic treated ginseng leaf by using Ultraflo L to improve the recovery of ginsenosides from the ginseng leaf and studied the biological activities and skin safety of the enzymatic treated ginseng leaf for use as a cosmetic material. The total ginsenoside contents of the non-enzymatic treated ginseng leaf (NEGL) and Ultraflo L treated ginseng leaf (UTGL) were 271 and 406 mg/g, respectively. The level of metabolite ginsenosides (sum of Rg2, Rg3, Rg5, Rk1, compound K, Rh1, Rh2, and F2) was higher in UTGL (93.1 mg) compared to NEGL (62.4 mg) in one gram ginseng leaf extract. The increase in amounts of ginsenoside types in UTGL compared to NEGL was generally 140% to 157%. UTGL exhibited relatively higher 2,2-diphenyl-2-picrylhydrazyl hydrate ($IC_{50}$, 2.8 mg/mL) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt ($IC_{50}$, 1.6 mg/mL) radical scavenging activities compared to NEGL (4.8 mg/mL and 2.2 mg/mL). The UTGL group showed normalized hydrogen peroxide, lipid peroxidation and visual wrinkling grade induced-UVB exposure. The UTGL did not induce any adverse reactions such as erythema and edema on intact skin sites; however, some guinea pigs treated with UTGL on abraded skin sites showed very slight erythema. The primary irritation index (PII) score of UTGL was 0.05 and it was classified as a practically non-irritating material (PII, 0 to 0.5). In skin sensitization tests with guinea pigs, UTGL had a positive rate of skin sensitization at 40%, and the mean evaluation score was 0.4.

• Journal of Ginseng Research
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• v.7 no.1
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• pp.13-22
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• 1983

This study was conducted to observe the nutritoinal effect of the diets supplemented with the leaf or trunk of ginseng in rats. The male albino rats (110 heads), Sprague-Dowley strain weighing 75g to 79g, were used as the experimental animals. And twelve kinds of animal diets were prepared. The animals were divided into twelve diet groups and maintained with corresponding diet for 40 days, and then sacrificed. After sacrificing the animals, the contents of some chemical components in some organs and serum were analyzed. The results obtained are summarized as follows; 1) The lipid contents of the liver in the experimental diet groups added ginseng steamed leaf or trunk were significantly lower than those in the control group. And the cholesterol contents of liver in the diet groups supplemented with ginseng steamed 4% leaf and 2% trunk were very significantly lower than those in the control group. 2) The total protein contents of serum in each experimental diet group supplemented with ginseng steamed leaf or trunk were lower than those in the control group, but not significant. 3) The glucose contents of serum in each experimental diet group supplemented with ginseng steamed leaf or trunk were lower than those in the control group, especially, those in experimental group added ginseng steamed 4% trunk were significantly lower than those in the control group. 4) The lipid contents of serum in the experimental diet groups added ginseng steamed 4% leaf and 2% trunk were significantly lower than those in the control group. The cholesterol of serum in the experimental diet groups added ginseng steamed leaf and 2% trunk were significantly lower those in the control group. 5) The ratios of ${\alpha}$-lipoprotein fraction in each experimental diet group were over than those in the group. but not significant.

• Korean Journal of Medicinal Crop Science
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• v.4 no.3
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• pp.255-260
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• 1996

This study was conducted to investigate the characteristics of aboveground plants and red ginseng of polystem ginseng in 6 years of age having two or more stems in a plant. Total leaf weight and area of polystem ginseng were larger, while its stem diameter, and the leaf weight and area of the big­gest stem in each plant were decreased with increase the stem numbers in a plant. The ratio of shoot weight to root weight in the polystem ginseng with three or more stems was higher than that in the monos­tem ginseng and the polystem ginseng with two stems, In ginseng plants with no more than 2 stems, there were positive correlations between root weight and total leaf weight, and leaf area, but not between leaf weight and area of the biggest stem. Inner cavity and inner white, limiting factors for redginseng quality grade, occurred more in tri-stem ginseng than mono- and di-stem one. Percentages of Heaven (1st grade) and Earth (2nd grade) red ginseng in tri-stem ginseng were decreased compared with mono stem and di-stem ginseng.

• Journal of Ginseng Research
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• v.11 no.2
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• pp.101-110
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• 1987

This study was investigated and analyzed the physiological reaction mechanisms and the factors of the chlorophyll bleaching phenomenon on leaf burning-disease of the Ginseng (Panax ginseng C.A. Meyer). Chlorophyll bleaching phenomenon was mainly caused by the photooxidation of singlet oxygen and the autooxidation of hydrogen peroxide($H_2O_2$) accumulation resulted from inactivation of catalase and peroxidase. Chlorophyll bleaching phenomenon was remarkably accelerated by addition of saponin.

• Proceedings of the Ginseng society Conference
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• 1990.06a
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• pp.149-154
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• 1990

Various rates of 2, 4-2 were sprayed on 2 and 3 year old ginseng plants as fouler spray to define the critical concentration. No apparent plant injury was noticeable for those ginseng plants when application concentration of 2, 4-D doubled the recommended dosage (70 mil 10a). Neither abnormal fouler change occurred nor any inhibition in leaf and stem growth was resulted for the plants treated with 2, 4-D concentrated two times of the recommended dosage. When the rates of 2, 4-D application were increased greater than this level, injury ratings increased linearly with the rates of 2, 4-D application and plant growth was inhibited. Ethylene gas was not produced from the ginseng plant treated with 2 times concentrated 2, 4-D, how- ever the ginseng plants produced 0.03 to 0.09 ppd ethylene gas when the rate of application were increased 3 and 4 times, respectively. On the other hand the soybean treated with the recommended amount of 2i-D produced ethylene gas of 10-20 times higher compared with ginseng plants and died. Photosynthesis ability of the ginseng leaf was significantly decreased by 2, 4.D fouler application but it was recovered 4 weeks after 2, 4-D fouler treatment. The herbicide 2, 4-D was appreciated to 2, 3 and 4 years old ginseng plants as fouler spray with the rates of 0.5, 1.0, 1.5 and 2.0 times of the recommended dosage to define the effects of 2, 4-D on the plant growth and root yield of the ginseng, There were no significant differences in the leaf and stem growth between untreated and 2, 4-D treated plant. Berry maturing of 3 and 4 year old ginseng was not influenced by 2, 4-D. The root weight of 4 years old ginseng plant was not reduced b). application of 2, 4-D concentrated 2 times of the recommended dosage. Application time of the herbicide 2, 4-D had no effects on the leaf or stem growth of 2, 3 and 4 year old year old ginseng plants. When the ginseng seedling was treated with 2, 4-D, detrimental phenomena as stem bending and deceleration of seedling leaf margin occurred, but stem bending was recovere d in a few day.

• The Korean Journal of Food And Nutrition
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• v.11 no.1
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• pp.82-87
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• 1998

To study of production of Panax ginseng leaf tea, after harvested the leaves in July, August, and September as ripening season, the content and composition of ginseng saponin were investigated. 1. Crude saponin contents in the leaves were a about 16.5%, and they were found to be lower in the leaf harvested in September than those harvested in July or August. 2. As similar patterns were observed with month to month in ginsenoside, sum of major ginsenosides of -Re, -Rd and -Rg1 was fixed about 70% of saponin at harvested in each month. And minor components were ginsenoside -Rb1, -Rb2 and -Rc as in order. 3. The ratio of protopanaxadiol(PD)/protopanaxatriol(PT) was revealed reduction of 1.13 of harvested in July to 0.85 of those in September gradually. The contents of protopanaxadiol were high in the leaves of August and protopanaxatriol was high in those September.