• Journal of Integrative Natural Science
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• v.9 no.3
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• pp.161-165
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• 2016

The p21-activated kinase-1 (PAK1) has emerged as a potential target for anticancer therapy. It is overexpressed in ovarian, breast and bladder cancers. This suggests that PAK1 may contribute to tumorigenesis. 4-azaindole derivatives are reported as potent PAK1 inhibitors. The present work deals with the molecular docking studies of 4-azaindoles with PAK1. Probable binding mode of these inhibitors has been identified by molecular modeling. Docking results indicated that hydrogen bonding interactions with Glu345 and Leu347 are responsible for governing inhibitor potency of the compounds. Additionally, Val284, Val328, Met344 and Leu396 were found to be accountable for hydrophobic interactions inside the active site of PAK1.

• Korean journal of food and cookery science
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• v.30 no.5
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• pp.588-595
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• 2014

To develop a variety of salted vegetables, this study investigated the quality properties and sensory characteristics of pak-choi Baikkimchi with 0, 1, 3, 5 and 7% (w/w) red pepper seed during 50 days at $10^{\circ}C$. The pH of pak-choi Baikkimchi decreased little for the first days but decreased significantly after day 20. The larger amount of the red pepper seed kept pH the less affected. The total acidity of pak-choi Baikkimchi increased within 20 days. Lactic acid bacteria count, pak-choi Baikkimchi with 0% red pepper seed decreased after 30 days' increase, while pak-choi Baikkimchi with 3% and 5% red pepper decreased significantly after 20 days' increase. Hunter's color L value increased during storage but a and b value decreased. Pak-choi Baikkimchi with 0%, 5% and 7% red pepper seed on day 10, pak-choi Baikkimchi with 1% 3% red pepper seed on day 20. Flavor preference was higher on day 20 of storage. In taste analysis, pak-choi Baikkimchi with 3% and 7% red pepper seed higher preference on day 10 and with 5% on day 30. Appearance preference was higher for pak-choi Baikkimchi with 0% and 3% red pepper seed on day 10 and with the others on day 20. Texture preference decreased as the storage time. Overall preference of pak-choi Baikkimchi with 0%, 1% and 7% were higher on day 10, pak-choi Baikkimchi with 3% and 5% red pepper seed were higher on day 20. In conclusion, the quality properties and sensory characteristics of pak-choi Baikkimchi were optimal on day 20 of storage and 3% red pepper seed.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.12 no.6
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• pp.113-124
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• 2002

Proposals for a password-based authenticated key exchange protocol that have been published so far almost concentrated on the provable security. But in a real environment such as mobile one, efficiency is a critical issue as security. In this paper we discuss the efficiency of PAK which is secure in the random oracle model ［l］. Among 4 hash functions in PAK the instantiation for $H_1$, which outputs a verifier of the password, has most important effect on the computational efficiency. We analyze two different methods for $H_1$ suggested in ［1］ and we show that $H_{lq}$ has merits in transforming to EC or XTR variants as well as in the efficiency. As an efficient variant. we propose PAK2-EC and PAK2-XTR which do not require any additional step converting a hash output into a point of elliptic curve or XTR subgroup when compared to the previous work on the PAK［2］. Finally we compare PAK2 with the password-based authenticated key exchange protocols such as SPEKE, SRP, and AMP.

• The Korean Journal of Physiology and Pharmacology
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• v.16 no.3
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• pp.159-165
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• 2012

Squamous cell carcinoma (SCC) and adenocarcinoma (AC) are the major histological types of non-small cell lung carcinoma (NSCLC). Although both SCCs and ACs have been characterized histologically and clinically, the precise mechanisms underlying their migration and invasion are not yet known. Here, we address the involvement in NSCLC of the p21-associated kinase1 (Pak1)/LIM kinase1 (LIMK1)/cofilin pathway, which recently has been reported to play a critical role in tumor migration and invasion. The Pak1/LIMK1/cofilin pathway was evaluated in tumors from SCC (n=35) and AC (n=35) patients and in SCC- and AC-type cell lines by western blotting, immunohistochemistry, and in vitro migration and invasion assays. The levels of phosphorylated Pak1, LIMK1, and cofilin in lung tumor tissues from SCC patients were increased as compared to normal tissues. In addition, immunohistochemistry showed greater expression of phosphorylated cofilin in SCC tissues. Expression of phosphorylated Pak1 and LIMK1 proteins was also significantly higher in SCC-type cells than in AC-type cells. Moreover, migration and invasion assays revealed that a higher percentage of SCC type cells exhibited migration and invasion compared to AC type cells. Migration was also decreased in LIMK1 knockdown SK-MES-1 cells. These findings suggest that the activation of the Pak1/LIMK1/cofilin pathway could preferentially contribute to greater tumor migration and invasion in SCC, relative to that in AC.

• Mycobiology
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• v.37 no.1
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• pp.69-71
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• 2009

Clubroot symptoms occurred severely on roots of Pak-Choi (Brassica campestris ssp. chinensis) grown in greenhouses in Gwangju city, Gyeonggi province, Korea in September, 2008. The incidence of the disease symptoms reached as high as 90% in three greenhouses investigated. The root galls collected from the greenhouses were sectioned using a scalpel and observed by light microscope. Many resting spores were found in the cells of the root gall tissues. Suspension of resting spores was prepared from the root galls and inoculated to roots of healthy Pak-Choi plants. Each of five resting spore suspensions caused clubroot symptoms on the roots, which were similar to those observed during the greenhouse survey. Resting spores of the pathogen were observed in the cells of the affected roots. The clubroot pathogen was identified as Plasmodiophora brassicae based on its morphological and pathological characteristics. This is the first report that Plasmodiophora brassicae causes clubroot of Pak-Choi.

• 대한약리학회:학술대회논문집
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• 2006.11a
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• pp.63-63
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• 2006

• Journal of Food Hygiene and Safety
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• v.1 no.2
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• pp.171-176
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• 1986

ABSTRACT-In order to detect the aflatoxins in home-made Takju and peanut butter, the samples were collected in Chungbuk region and cleaned up Sep-pak silica cartridge. Aflatoxins were detected by thin layer chromatographic and high performance liquid chromatographic behavior. Determination was carried out by thin layer densitometer. The results were as follows; 1. Aflatoxin B, was detected in 78% of the home-made Takju, and the highest concentration was 1.2 ppb and average 0.36 ppb. 2. Aflatoxins were not detected in any peanut butter smaples. 3. Clean-up method by Sep-pak silica cartridge was more efficient and economical than column chromatography of AOAC method.method.

• Bulletin of the Korean Chemical Society
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• v.11 no.1
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• pp.28-31
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• 1990

By using Pak's theory of liquid, a phenomenological theory of viscosity proposed by the authors is applied to liquids under high pressures. The calculated viscosities for various simple substances are in good agreements with those of the observed values over wide pressure ranges.

• Journal of Bio-Environment Control
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• v.28 no.4
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• pp.342-351
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• 2019

The optimum N concentrations incorporated as pre-planting nutrient charge fertilizer were determined for seedling raising using cylindrical paper pots. A root medium was formulated by blending of peat moss (particles smaller than 2.84 mm were 80-90%) and perlite (1 to 3 mm) with the ratio of 7:3 (v/v). The treatment N concentrations incorporated during the root medium formulation were adjusted to 0, 150, 250, 500, and $750mg{\cdot}L^{-1}$ and the concentrations of essential nutrients except N were equal in all treatments. After making of paper pots and putting into the 40-cell tray, the seeds of Chinese cabbage ('Chunmyeong Bom Baechu') and pak-choi ('Hanog cheonggyeongchae') were sown. During the raising of seedlings, weekly analysis of medium pH, EC and concentrations of inorganic elements were conducted. After 21 and 20 days after seed sowing of Chinese cabbage and pak-choi, the growth of the above-ground parts were measured and contents of inorganic elements in the plant tissues were analyzed. During the growing period, pH of the root media rose gradually and the EC decreased rapidly at week 3. The pH of root media at harvest was in the range of 5.3 to 5.9 in Chinese cabbage and 4.93 to 5.39 in pak-choi. Growth of the aboveground parts in terms of fresh and dry weight in both the plants were the highest in the $250mg{\cdot}L^{-1}$ N treatment and the lowest in the control treatment. The elevation of pre-planting N concentrations in root medium resulted in the increase of tissue N content and decrease of P, Ca, and Mg contents. The regression equation derived from the influence of varied pre-planting N concentrations on dry weight of above-ground tissue were $y=-0.0036x^2+0.0021x+0.0635$ ($R^2=0.9826$) in Chinese cabbage and $y=-0.16x^2+0.0009x+0.032$ ($R^2=0.991$) in pak-choi. When the low critical concentration of pre-plant N is taken at the point where dry weight of above-ground tissue is 10% less than maximum (0.40 g in Chinese cabbage and 0.16 g in pak-choi), those point are 0.36 g and 0.144 g per plant in Chinese cabbage and pak-choi, respectively. The lower critical N concentrations of root media calculated from the regression equations are $196mg{\cdot}L^{-1}$ for Chinese cabbage and $187mg{\cdot}L^{-1}$ for pak-choi. These results indicate that optimum pre-plant N concentrations for seedling raising using paper pots are in the range of 196 to $250mg{\cdot}L^{-1}$ for Chinese cabbage and 187 to $250mg{\cdot}L^{-1}$ for pak-choi.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.2
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• pp.245-252
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• 2011

The combined effects of 10 kJ/$m^2$ ultraviolet-C (UV-C) with 50 ppm aqueous chlorine dioxide ($ClO_2$) on the qualities of red chicory and pak choi were examined. After the treatment, the samples were stored at $4{\pm}1^{\circ}C$ for 7 days. The combined treatment of $ClO_2$/UV-C reduced the initial populations of total aerobic bacteria in the red chicory and pak choi by 2.64 and 2.55 log CFU/g, respectively, compared to those of the control. Also, the populations of yeast and molds in the red chicory and pak choi by combined treatment were reduced by 2.41 and 2.00 log CFU/g, respectively. In addition, after 7 days of storage the total aerobic bacteria populations in the red chicory and pak choi were reduced to 3.71 and 3.93 log CFU/g, compared to 6.31 and 6.62 log CFU/g for the control, resulting in a significant decrease. Hunter L, a, and b values of red chicory and pak choi were not significantly different among the treatments. Non-thermal treatment caused a negligible change in sensory evaluation. These results suggest that the combined treatment of 50 ppm $ClO_2$ with 10 kJ/$m^2$ UV-C can be useful for maintaining the qualities of red chicory and pak choi.