• Journal of Nutrition and Health
• /
• 제54권1호
• /
• pp.95-103
• /
• 2021

Purpose: High intake of cholesterol leads to cardiovascular disruption. Estimating the actual intake of cholesterol can be beneficial for nutrition intervention. This research aimed to develop Semi-Quantitative Food Frequency Questionnaire (SQ-FFQ) to estimate cholesterol intake and analyze its reliability and validity. Methods: SQ-FFQ was developed by sorting high cholesterol food items in Indonesian food database and food items' availability. A total of 30 older adults were randomly chosen from Public Health Center in Jagir District, Surabaya, Indonesia to test its validity. Reliability test was done by measuring the same developed SQ-FFQ in one-month period, while validity test was done by comparing SQ-FFQ results with 6-days food record. Statistical analysis used for reliability test was paired t-test, the Intra-class Correlation Coefficient (ICC), and Cronbach's α to measure the internal consistency. Meanwhile, validity of developed SQ-FFQ was analyzed using paired t-test and Bland-Altman. Results: Reliability of 2 administered SQ-FFQs showed a good agreement based on paired t-test analysis (p = 0.200), ICC (0.609), and Cronbach's α (0.757). Strong agreement was found in most of food items, but agreements for egg yolk and fried duck were poor. Significant difference was found between those food items (p = 0.001 vs. p < 0.001, respectively) with mean difference were -25.3 mg and 46.2 mg. Validity of developed SQ-FFQ2 compared to 6-days food diary records also found a strong agreement based on paired t-test and the Bland-Altman analysis. Conclusion: This baseline research provides a reasonably valid and repeatable measure of cholesterol intake estimation that can be widely used in nutrition and public health study, especially in Indonesia. No study has been conducted in Indonesia on the development of tools to estimate the cholesterol intake.

• 대한소아치과학회지
• /
• 제27권1호
• /
• pp.7-14
• /
• 2000

가바성 억제 신경세포는 해마의 정상적인 기능을 조절하는데 중요한 역할을 하며 해마 병변을 유발하는 중요한 요소이다. 본 연구는 in vivo 실험법을 사용하여 해마 CA1 영역에서의 전기 생리학적 반응을 측정함으로써 가바성 신경세포의 기능을 분석하고 이를 슬라이스 실험법과 비교하고자 하였다. Fimbria-fonix 전기자극시 전형적인 population spike가 나타났고 $10{\sim}M$ bicuculline 존재하에서는 전기자극에 의해 burst 형태의 population spike가 나타났다. Population spike의 크기는 자극 강도에 비례하였으며 그 숫자도 bicuculline 전극사용시와 같이 동일한 양상을 보였다. CA1 영역의 흥분성 수준을 측정하기 위해 paired-pulse 자극을 하였는데 짧은 자극 간격에서 억제성 반응을 보였고 burst형태의 afterdischarge를 나타내었다. CA1 영역에서 in vivo실험법을 사용한 가바성 신경세포반응의 결과는 추체세포의 흥분성 조절을 효과적으로 분석할 수 있으며 in vitro 실험법에 비해 기능적 평가가 더욱 이상적임을 알 수 있다.

• 한국소음진동공학회논문집
• /
• 제15권8호
• /
• pp.945-953
• /
• 2005

The purpose of this paper is to develop a linear regression model for the sound quality index of vehicle Interior noise. For this, objective measurement data of the vehicles driving in acceleration was measured. On the basis of analysis, psychoacoustic parameters were extracted and subjective evaluation was performed by noise and vibration expert evaluators. For the subjective evaluation, the paired comparisons and the semantic differential methods were used to evaluate sound quality of vehicle interior noise. By the paired comparison which evaluate two pairs of vehicle interior noise, the preference was estimated. With the semantic differential and the factor analysis, it was evaluated words of two pairs which expressed appropriately the sense of evaluator about noise source. Therefore the characteristics of the sound qualify for the vehicle were differentiated. From the results of both the correlation analysis and the multiple factor regression analysis, the sound quality evaluation model for the sense of human hearing was derived and indexed.

• 지역사회간호학회지
• /
• 제13권1호
• /
• pp.5-16
• /
• 2002

Purpose: This study was to compare the stop-smoking rate and nicotine dependency for an adult smoker after implementing the smoking cessation program. Method: Fourty-six adult smokers participated. Self report questionnaire and telephone interview were conducted at the pretest, 6 months later, 1 year later, 2 years later, 3 years later. Result: 1. Stop-smoking rate of smoking cessation program participants was 43.5% 6 months later, 35.6% 1 year later, 24.4% 2 years later, 45.5% 3 years later. 2. The mean of nicotine dependency for continuing smokers was significantly decreased 6 months later(paired-t=4.719, p=.000). 3. The mean of nicotine dependency for continuing smokers was significantly decreased 1 year later(pired-t=4.296=.000). 4. The mean of nicotine dependency for continuing smokers was significantly decreased 2 years later(paired-t=2.778. p=.000). 5. The mean of nicotine dependency for continuing smokers was significantly decreased 3 years later(paired-t=2.795. p=.000). Conclusion: The smoking cessation program was effective to induce cessation in the adult smoker, and to decrease nicotine dependency in the continuing adult smoker.

• 미생물학회지
• /
• 제32권1호
• /
• pp.28-33
• /
• 1994

정상적인 세포에서 tau 단백질은 신경세포의 축색돌기에 있는 미세소관(microtubule)과 결합하고 있지만, Alzheimer병 세포의 경우 그 단백질은 몇몇 신경세포의 체세포 수지상조직(somatodendrite) 부위에 고착되어서 이중나선 섬유(paired helical filament; PHF)의 주성분을 이루게 된다. 따라서 뇌에PHF가 축적되는 특성 파악과 그들을 야기시키는 요인분석의 일환으로 다량의 순수한 tau 단백질을 확보하기 위하여 본 연구에서는 인체 tau 유전자의 cDNA를 클로닝하고 Escherichia coli에서의 발현을 유도하였다.

• 한국환경과학회지
• /
• 제20권4호
• /
• pp.493-500
• /
• 2011

This study investigates the relationship between Asian dust and asthma disease in Seoul, using data of Asian dust occurrences and the number of treatments for asthma between 2005 and 2008. The data include the number of treatments for asthma on the basic day paired with the average number of treatments for asthma on the compared days. The compared day is defined by the day of no Asian dust in same month and day of the week as the basic day, when Asian dust occurs. Also, the basic day is expanded to the day after three days from the day of Asian dust. The paired two sample t-test for the number of treatments for asthma on the basic day and the compared days revealed that the Asian dust occurrences are correlated with the asthma disease. The number of treatments for asthma is significantly increased on the one and two days after Asian dust occurs. On the other hand, there's no significant difference in the number of treatments for asthma between the days of Asian dust occurrence and the days of no Asian dust, which implies that people usually try not to go out when Asian dust occurs.

• 정보보호학회논문지
• /
• 제27권6호
• /
• pp.1483-1490
• /
• 2017

스마트워치는 사용자가 착용하는 소형 스마트 기기로 연결성, 기능성 및 유용성을 위해 호스트 기기인 스마트폰과 페어링 과정을 필요로 한다. 페어링 과정 이후에는 사용자의 의식 없이도 스마트워치와 스마트폰 기기 간의 데이터 업데이트 및 백업 과정이 가능하며 스마트폰의 다양한 데이터가 복제되어 스마트워치로 넘어온다. 본 연구는 플랫폼별 스마트워치에 대해 스마트폰으로부터 복제되어 넘어오는 데이터를 사용 시점별로 추출 및 관찰하여 분석한다. 또한 스마트워치 사용자를 대상으로 실시한 보안관련 인식에 대한 설문조사를 바탕으로 데이터 추출 실험 결과에 따른 보안 및 개인 정보보호관점에서의 데이터별 위험도 평가를 수행하고 대응방안을 고찰한다.

• International Journal of Oral Biology
• /
• 제46권3호
• /
• pp.146-153
• /
• 2021

Accurate identification of microbes facilitates the prediction, prevention, and treatment of human diseases. To increase the accuracy of microbiome data analysis, a long region of the 16S rRNA is commonly sequenced via paired-end sequencing. In paired-end sequencing, a sufficient length of overlapping region is required for effective joining of the reads, and high-quality sequencing reads are needed at the overlapping region. Trimming sequences at the reads distal to a point where sequencing quality drops below a specific threshold enhance the joining process. In this study, we examined the effect of trimming conditions on the number of reads that remained after quality control and chimera removal in the Illumina paired-end reads of the V3-V4 hypervariable region. We also examined the alpha diversity and taxa assigned by each trimming condition. Optimum quality trimming increased the number of good reads and assigned more number of operational taxonomy units. The pre-analysis trimming step has a great influence on further microbiome analysis, and optimized trimming conditions should be applied for Divisive Amplicon Denoising Algorithm 2 analysis in QIIME2 platform.

• 한국동물생명공학회지
• /
• 제36권4호
• /
• pp.314-322
• /
• 2021

Paired box protein, PAX7, is a key molecule for the specification, maintenance and skeletal muscle regeneration of muscle satellite cells. In this study, we identified and characterized the cDNA and amino acid sequences of PAX7 from black sea bream (Acanthopagrus schlegelii) via molecular cloning and sequence analysis. A. schlegelii PAX7 cDNA was comprised of 1,524 bp encoding 507 amino acids and multiple sequence alignment analysis of the translated amino acids showed that it contained three domains including paired DNA-binding domain, homeobox domain and OAR domain which were well conserved across various animal species investigated. Pairwise Sequence Alignment indicated that A. schlegelii PAX7 had the same amino acid sequences with that of yellowfin seabream (A. latus) and 99.8% identity and similarity with that of gilt-head bream (Sparus aurata). Molecular phylogenetic analysis confirmed that A. schlegelii PAX7 formed a monophyletic group with those of teleost and most closely related with those of the fish that belong to Sparidae family including A. latus and S. aurata. In the investigation of its tissue specific mRNA expression, the expression was specifically identified in skeletal muscle tissue and a weak expression was also shown in gonad tissue. The cultured cells derived from skeletal muscle tissues expressed PAX7 mRNA at early passage but the expression was not observed after several times of subculture.

• 한국과학교육학회지
• /
• 제23권1호
• /
• pp.57-65
• /
• 2003

이 연구에서는 계획과 검토 단계를 강조한 4단계 문제 해결 전략을 사용하는 해결자 청취자 활동에서 내 외향성에 따른 소집단 구성의 효과를 조사하였다. 수업 전 고등학교 이과 2학년 3개 학급(N=87)을 대상으로 내 외향성 검사를 실시한 후, 내 외향성 동질 집단과 이질 집단, 그리고 통제 집단으로 무선 배치하였다. 연구 결과, 화학 문제 해결력 검사의 점수는 두 처치 집단이 통제 집단보다 유의미하게 높았으나, 학습 곤란도와 자야효능감에서는 집단 간 유의미한 차이가 없었다. 수업 처치에 대한 의식의 하위 항목들에서는 두 처치 집단 간 유의미한 차이가 없었으나, 청취자의 역할 수행에 대한 인식, 문제 해결 전략과 해결자 청취자 활동에 대한 선호도에서 외향적인 학생의 점수가 내향적인 학생의 점수에 비해 유의미하게 높았다.