• 제목/요약/키워드: Paecilomyces lilacinus

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더덕(Codonopsis lanceolata) 재배지에서 당근뿌리혹선충(Meloidogyne hapla)의 생물적 방제 (Biological Control of the Northern Root-Knot Nematode, Meloidogyne hapla in the Fields of Codonopsis lanceolata)

  • 정도철;한상찬
    • 한국응용곤충학회지
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    • 제43권1호
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    • pp.27-34
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    • 2004
  • 더덕(Codonopsis lanceolata) 재배지에서 당근뿌리혹선충의 환경친화적 방제법을 구명코자 방제실험을 수행한 결과 당근뿌리혹선충 방제 실험에 사용된 방제제들(Bacillus thuringiensis, Paecilomyces lilacinus, fosthiazate, 한약제 추출물) 중 더덕의 발아를 향상시키는 것을 Bt와 한약제 추출물이었고, 억제시키는 것은 fosthiazated였다. Pot에서 미생물제에 의한 당근뿌리혹선충 밀도억제 효과는 Bt와 Paecilomyces lilacinus 모두 우수하였으나 fosthiazate 보다는 떨어지는 경향이었다. 또한 한약제 추출물도 방제효과가 우수하였다. 포장실험에서도 Paecilomyces lilacinus와 fosthiazate 처리 시 수확량이 가장 많았으며 수확된 더덕의 품질도 가장 우수하였다. 한약제추출물도 무처리에 비하여 더덕의 수량 증대에 효과가 있었고, 뿌리혹선충 감염지에서 키운 더덕을 이식한 것이 비감염지에서 키워 이식한 더덕에 비하여 수량 증가율이 저조하였다.

Meloidogyne incognita 알을 제어하는 Paecilomyces lilacinus의 현미경적 관찰 (Microscopic observation of Paecilomyces lilacinus that have control over Meloidogyne incognita eggs)

  • 이광배
    • 환경위생공학
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    • 제22권3호
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    • pp.27-33
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    • 2007
  • M. incognita are plant-parasite nematode that cause severe damage to the crops. P. lilacinus are renowned for inhibitation of development of M. incognita's egg. We make a study for enzymatic examining the cause closely that P. lilacinus suppress development of M. incognita's egg by parasiting. The research result is explained the place below. 1. The egg that is exposed to co-enzymes which are cultured in the broth culture starts to change the membrane of egg from 3days. And in 10days, that membrane completely disappear. These are observed through light microscope. Therefore, we know that M. incongnita are controlled by extracellular lytic enzymes that are produced by P. lilacinus. 2. Through scanning electron microscope, we can find that the egg that is attacked by P. lilacinus loses it's membrane gradually, and that loss of the membrane causes transform, which suppresses the development of egg.

Preliminary Evaluation of Paecilomyces lilacinus HY-4 to Control Tetranychus urticae

  • Shin, Tae-Young;Lee, Won-Woo;Ko, Seung-Hyun;Ji, Zhen;Shin, Dong-Ha;Son, Kwang-Hee;Park, Ho-Yong;Woo, Soo-Dong
    • International Journal of Industrial Entomology and Biomaterials
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    • 제22권1호
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    • pp.25-28
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    • 2011
  • The virulence against the two-spotted spider mite, Tetranychus urticae Koch, was evaluated with entomopathogenic fungus Paecilomyces lilacinus (Thom) Samson HY-4 which is isolated from coleopteran insect and registered to Korean and US patents. Virulence tests were conducted with conidial suspensions ($1.5{\times}10^6conidia/ml$) of P. lilacinus HY-4 against T. urticae adults and positive results were recorded in laboratory conditions. The spraying device was also developed for the efficient and exact evaluation of treatment. The developed spraying device was named as SD-tower sprayer and its efficacy of spraying conidia was evaluated. The accumulated mortality caused by P. lilacinus HY-4 using SD-tower spray was about 73% at 6 days after inoculation. This suggest that the isolate P. lilacinus HY-4 may be considered as promising for a new approach to prevent adult infestations by T. urticae.

α-Pyrones and Yellow Pigments from the Sponge-Derived Fungus Paecilomyces lilacinus

  • Elbandy, Mohamed;Shinde, Pramod B.;Hong, Jong-Ki;Bae, Kyung-Sook;Kim, Mi-Ae;Lee, Sang-Mong;Jung, Jee H.
    • Bulletin of the Korean Chemical Society
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    • 제30권1호
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    • pp.188-192
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    • 2009
  • New $\alpha$-pyrones (1 and 2) and cyclohexenones (13 and 14) were isolated along with known analogues (3, 5−12) from the ethyl acetate extract of the whole broth of the fungus Paecilomyces lilacinus, a strain derived from a marine sponge Petrosia sp. Their structures were established by interpretation of 1D and 2D NMR, and FABMS data. It is interesting to isolate cyclohexenone derivatives from the genus Paecilomyces (family Trichocomaceae, order Eurotiales), since these cyclohexenones were previously reported only from far distinct genera, Phoma and Alternaria (family Pleosporaceae, order Pleosporales). Compounds 6, 7, and 9 were evaluated for cytotoxicity against a small panel of human solid tumor cell lines. Their cytotoxicity was insignificant upto a concentration of 30 ${\mu}g/mL$.

곰팡이(Paecilomyces lilacinus)를 이용(利用)한 고추 및 상치의 당근뿌리혹선충(線蟲)(Meloidogyne hapla)의 생물학적(生物學的) 방제(防除)에 관(關)하여 (Biological Control of Meloidogyne hapla on Lettuce and Pepper by Paecilomyces lilacinus)

  • 조명래;최영연
    • Current Research on Agriculture and Life Sciences
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    • 제8권
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    • pp.65-70
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    • 1990
  • 선충기생(線蟲寄生)곰팡이(Paecilomyces lilacinus)를 이용(利用)한 당근뿌리혹선충(線蟲)(Meloidogyne hapla)의 생물학적방제(生物學的防除) 가능성(可能性)을 규명(糾明)하기 위(爲)하여 Telone II, Telone C-17, Dowfume MC-II 등(等) 3종(種)의 토양훈증제(土壤燻蒸劑)와 함께 밀양(密陽)의 시설원예포장(施設園藝圃場)에 처리(處理)하여 상치와 고추를 전후작실험(前後作實驗)한 결과(結果), 뿌리혹선충(線蟲) 밀도(密度)는 처리후(處理後) 25일(日)에 P.lilacinus 처리구(處理區)는 22마리, Telone II, Telone C-17, Dowfume MC-II구(區)는 각각(各各) 2, 24, 0마리로 무처리구(無處理區)의 685마리에 비(比)해 현저(顯著)히 낮았으며 처리후(處理後) 350일(日)에는 P. lilacinus, Telone II, Telone C-17, Dowfume MC-II구(區) 각각(各各) 2,917, 173, 180, 361마리로 무처리구(無處理區) 8,635마리에 비(比)해 각각(各各) 66%, 98%, 98%, 96%의 방제효과가 있었다. 전작물(前作物)인 상치는 P.lilacinus, Telone II, TeloneC-17, Dowfume MC-II구(區)에서 각각(各各) 49, 43, 31, 109% 증수(增穗)되었다. 고추의 생체중(生體重)과 초장(草長)은 훈증제(燻蒸劑) 처리구(處理區)에서는 모두 높게 나타났으나 P. liacinus처리구(處理區)는 무처리구(無處理區)에 비(比)하여 차이(差異)가 없었다.

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몇종의 선충천적 진균과 화학약제를 이용한 약용작물 뿌리혹선충 방제효과 검토 (Field Application of Egg and Larval Parasitic Fungi and Chemicals for Controlling Root-knot Nematodes on Some Medicinal Herb)

  • 박소득;추연대;정기채;심용구;최영연
    • 한국응용곤충학회지
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    • 제32권1호
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    • pp.105-114
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    • 1993
  • Series of studies were conducted to establish biological and chemical control method for Meloidogyne spp to medical herbs by applied of nematophagous fungi, Arthrobotrys spp, Fusarium spp, and egg parastic fungi, Paecilomyces lilacinus were applied for root-knot nematodes on medicinal herbs, Paeonia albiflora, Codonopsis lanceolata, Cnidium officinale. The results are as follow. In pot experiments, The no. of root gall and egg mass and larvae of Cnidium officinale. The results are as follow. In pot experiments. The no. of root gall and egg mass and larvae of Cnidium officinale, Codonopsis lanceolata, Paeonia japonica lowered in P. Lilacinus treated plots compare to untreated control plots. But A. thaumasia F. oxysporum treated plots were less effective. Effect of egg parasitic fungi and chemical treatment at divided root of Paeonia japonica after sterilized in pot were increased in the fresh weight, root weight, control effect in P. lilacinus treated plots as chemical, Carbo G treated plots compare to untreted control plots. I field experiment, the number of root gall, egg mass and nematode density of Paeonia were also suppressed in P. lilacinus treated plots. It was very effective continuous 2 years and transplanting time on Paeonia japonica infested soil with the M. hapla in field in both region, Chillgok and Euisung treated P. lilacinus as chemical treated plots. Soaking effect of insecticide for Paeonia japonica at diving shoot before transplating in pot were effective for 12hours immersion into 1,000 ppm of Benlate T + Mep Ec, Benlate T + Fenthion EC in pot and field Experiment. In the Examination of fungi activily on P.japonica field 1 year after soil treatment, Number of spore of P. lilacinus were 1,000~1,300 in 3 region except Euisung.

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Antimicrobial and Synergistic Effects of Silver Nanoparticles Synthesized Using Soil Fungi of High Altitudes of Eastern Himalaya

  • Devi, Lamabam Sophiya;Joshi, S.R.
    • Mycobiology
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    • 제40권1호
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    • pp.27-34
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    • 2012
  • Fifty three fungi isolated from soils of different microhabitats of eastern Himalayan range (3,400-3,600 msl) were screened for mycosynthesis of silver nanaoparticles (AgNPs) and their efficacy as antimicrobials were assessed in combination with commonly used antibiotics. Three isolates $Aspergillus$ $terreus$ SP5, $Paecilomyces$ $lilacinus$ SF1 and $Fusarium$ sp. MP5 identified based on morphological and 18S rRNA gene sequences were found to synthesize AgNPs. These nanoparticles were characterized by visual observation followed by UV-visible spectrophotometric analysis. The AgNPs synthesized by $Aspergillus$ $terreus$ SP5, $Paecilomyces$ $lilacinus$ SF1 and $Fusarium$ sp. MP5 showed absorbance maxima at 412, 419, and 421 nm respectively in the visible region. Transmission electron microscopy micrograph showed formation of spherical AgNPs of 5-50 nm size. The antimicrobial activity of the mycosynthesized nanoparticles were investigated alone and in combination with commonly used antibiotics for analysis of growth inhibition zone against test organisms, namely, $Staphylococcus$ $aureus$ MTCC96, $Streptococcus$ $pyogenes$ MTCC1925, $Salmonella$ $enterica$ MTCC735 and $Enterococcus$ $faecalis$ MTCC2729. The mycosynthesized nanoparticles showed potent antibacterial activity and interestingly their syngergistic effect with erythromycin, methicillin, chloramphenicol and ciprofloxacin was significantly higher as compared to inhibitions by AgNPs alone. The present study indicates that silver nanoparticles synthesized using soil borne indigenous fungus of high altitudes show considerable antimicrobial activity, deserving further investigation for potential applications.

Biological Control of Mulberry Root Knot Nematode Meloidogyne incognita by Trichoderma harzianum

  • Sukumar, J.;Padma, S.D.;Bongale, U.D.
    • International Journal of Industrial Entomology and Biomaterials
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    • 제8권2호
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    • pp.175-179
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    • 2004
  • Trichoderma harzianum-THN1 parasitising the egg masses of root knot nematode Meloidogyne incognita was isolated from galled mulberry roots and evaluated for its potential to control root knot disease. In pot experiments root galling was reduced and leaf yield increased significantly following soil treatment with T. harzianum-THN1. The extracts obtained from the soils inoculated with T. harzianum-THN1 drastically inhibited the hatching of nematode eggs and the effect was irreversible even after the eggs were transferred to fresh water. The fungus was equally effective in controlling the disease in nematode infested mulberry garden under field conditions which was significant over the most commonly used egg parasitic fungus Paecilomyces lilacinus. The disease reduction recorded with T. harzianum was on par with the plants treated with the nematicide Carbofuran. The results suggest that T. harzianum- THN1 could be used as a potent ecofriendly biocontrol agent against M. incognita in mulberry without any residual toxicity to silkworms. T. harzianum- THN1 can form an important component of integrated disease management package in mulberry cultivation.