• 한국산림과학회지
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• 제95권5호
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• pp.585-590
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• 2006

In vitro-grown axillary shot-tip meristems of Lycium chinense Mill. from cold-acclimated plant were successfully cryopreserved using a vitrification technique. After loading for 15 minutes with a mixture of 2.0 M glycerol and 0.4 M sucrose ($20^{\circ}C$), small segments (1-2 mm, 3-4 mm, and 5-6 mm) were cut from axilary buds and exposed to the cryoprotectant solution containing 30% glycerol, 15% ethylen glycol,15% dimethyl sulfoxide (DMSO), and 0.4 M sucrose at $0^{\circ}C$ for 30-120 minutes prior to direct plunge into liquid nitrogen (LN). After rapid thawing ($40^{\circ}C$), the segments were washed with MS medium containing 1.2 M sucrose for 0-35 minutes, and then transferred onto recovery-growth medium. The highest survival rate (about 90%) was obtained with cold-hardening treatment, and cryopreserved explants were successfully recovered to plantlets. No abnormal morphological changes were observed with the recovered plants after cryopreservation.

• KEPCO Journal on Electric Power and Energy
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• 제7권1호
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• pp.171-177
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• 2021

In terms of distribution planning, accurate electric load prediction is one of the most important factors. The future load prediction has manually been performed by calculating the maximum electric load considering loads transfer/switching and multiplying it with the load increase rate. In here, the risk of human error is inherent and thus an automated maximum electric load forecasting system is required. Although there are many existing methods and techniques to predict future electric loads, such as regression analysis, many of them have limitations in reflecting the nonlinear characteristics of the electric load and the complexity due to Photovoltaics (PVs), Electric Vehicles (EVs), and etc. This study, therefore, proposes a method of predicting future electric loads on distribution lines by using Machine Learning (ML) method that can reflect the characteristics of these nonlinearities. In addition, predictive models were developed based on actual data collected at KEPCO's existing distribution lines and the adequacy of developed models was verified as well. Also, as the distribution planning has a direct bearing on the investment, and amount of investment has a direct bearing on the maximum electric load, various baseline such as maximum, lowest, median value that can assesses the adequacy and accuracy of proposed ML based electric load prediction methods were suggested.

• International Journal of Computer Science & Network Security
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• 제22권1호
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• pp.175-182
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• 2022

This work presents a simulation model for a specific UV disinfection system (UVDS) powered by a Photovoltaic System. The global UVDS also includes the electronic converters, Electronic Ballast, UV Lamp and Motor Pump. The equations that model the physical components' behaviour are connected to obtain a dynamic global model. The latter is converted in a Simulink/Matlab model, which allows to carry out simulation series concerning the entire UVDS. The physical parameters: the irradiation G and the temperature T, are considered as inputs. series of measurements carried out in order to show how these parameters affect the current, the voltage of the PVs and especially the value of the current of the UV lamp, on the other hand a study on the behavior and the evolution of the parameters of the motor pump such as the armature current, motor torque, speed of rotation and the water flow. The purpose of all this is to realize how important are the two parameters concerning the lamp current and the water flow because they are two very important factors to keep an adequate water quality.

• 대한치매학회지
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• 제22권3호
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• pp.87-99
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• 2023

Background and Purpose: According to the amyloid cascade hypothesis, fibrillary amyloid-beta load in the brain causes Alzheimer's disease (AD) with toxic effects. Recently, perivascular spaces (PVSs), fluid-filled cavities around small penetrating arterioles and venules in the brain, and the glymphatic system relationship with type 2 diabetes mellitus (DM2) and AD has been an important research topic from a physiopathological point of view. There are two types of PVSs that are associated with sporadic atherosclerosis and cerebral amyloid angiopathy. In this study, we evaluated the relationship between the number and localization of enlarged PVSs in AD. Methods: A total of 254 patients with AD and 125 healthy controls were included in this study All the patients were evaluated with neurological and cognitive examinations and magnetic resonance imaging (MRI). PVSs on MRI were graded by recording their number and location. The study was a retrospective study. Results: In our study, the number of white matter convexity-central semiovale localized PVSs was higher in patients than in the control group. In addition, the number of PVSs in this localization score was higher in patients with DM2. Cerebral PVS counts were higher in patients with AD than in the control group. Conclusions: These results suggest the important role of cerebral amyloid angiopathy, one of the vascular risk factors, and the glymphatic system in the pathogenesis of AD. In addition, the results of our study suggest that the evaluation of PVSs levels, especially at the (centrum semiovale), using imaging studies in AD is a potential diagnostic option.

• 한국자원식물학회지
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• 제32권6호
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• pp.689-697
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• 2019

This study describes an efficient and widely applicable droplet-vitrification following cryopreservation for shoot tips of strawberry (Fragaria × ananassa Duch.) cvs. 'Wonkyo3114' and 'Gurumi40'. The shoot tips were precultured in Murashige and Skoog (MS) liquid medium supplemented with sucrose (0.3-0.5M). Precultured explants were osmoprotected with loading solution (LS, C4) containing 20% glycerol and 20% sucrose for 40 min and exposed to dehydration solution (B5) containing 40% glycerol and 40% sucrose for 40 min at 25℃, Subsequently, the explants were transferred onto droplets containing 2.5 μL PVS3 on sterilized aluminum foils (4 cm × 0.5 cm) prior to direct immersion in liquid nitrogen (LN) for 1 h. The highest regrowth rate (%) in both the cultivars was obtained when the shoot tips were precultured with MS + 0.3M sucrose for 40 h at 25℃. The cryopreserved shoots tips exhibited 55% regrowth rate by culturing in NH₄NO₃-free MS medium supplemented with 3% sucrose, 1.0 g/L casein, 1.0 mg/L GA₃, and 0.5 mg/L BA for 5 weeks and in MS medium supplemented with 0.5 mg/L GA₃ for 8 weeks. This result shows that droplet-vitrification could be employed as a promising method for cryostorage of strawberry germplasm.

• 한국환경과학회지
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• 제16권9호
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• pp.1051-1061
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• 2007

The impact of midlatitude synoptic system (upper-level trough) on typhoon intensity change was investigated by analyzing the spatial and temporal characteristics of vertical wind shear (VWS), relative eddy momentum flux convergence (REFC), and potential vorticity (PV). These variables were computed over the radial mean $300{\sim}1,000km$ from the typhoon center by using GDAPS (Global Data Assimilation and Prediction System) data provided by the Korea Meteorological Administration (KMA). The selected cases in this study are typhoons Rusa (0215) and Maemi (0314), causing much damage in life and property in Korea. Results show that the threshold value of VWS indicating typhoon intensity change (typhoon to severe tropical storm) is approximately 15 m/s and of REFC ranges 6 to 6.5 $ms^{-1}day^{-1}$ in both cases, respectively. During the period with the intensity of typhoon class, PVs with 3 to 3.5 PVU are present in 360K surface-PV field in the cases. In addition, there is a time-lag of 24 hours between central pressure of typhoon and minimum value of VWS, meaning that the midlatitude upper-level trough interacts with the edge of typhoon with a horizontal distance less than 2,000 km between trough and typhoon. That is, strong midlatitude upper-level divergence above the edge of the typhoon provides a good condition for strengthening the vertical circulation associated with the typhoons. In particular, when the distance between typhoon and midlatitude upper-level trough is less than 1,000 km, the typhoons tend to weaken to STS (Severe Tropical Storm). It might be mentioned that midlatitude synoptic system affects the intensity change of typhoons Rusa (0215) and Maemi (0314) while they moves northward. Thus, these variables are useful for diagnosing the intensity change of typhoon approaching to the Korean peninsula.

• Advances in Energy Research
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• 제7권1호
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• pp.67-84
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• 2020

Given the recent surge of interest towards utilization of renewable distributed energy resources (DER), in particular in remote areas, this paper aims at designing an optimal hybrid system in order to supply loads of a village located in Esfarayen, North Khorasan, Iran. This paper illustrates the optimal design procedure of a standalone hybrid system which consists of Wind Turbine Generator (WTG), Photo Voltaic (PV), Diesel-generator, and Battery denoting as the Energy Storage System (ESS). The WTGs and PVs are considered as the main producers since the site's ambient conditions are suitable for such producers. Moreover, batteries are employed to smooth out the variable outputs of these renewable resources. To this end, whenever the available power generation is higher than the demanded amount, the excess energy will be stored in ESS to be injected into the system in the time of insufficient power generation. Since the standalone system is assumed to have no connection to the upstream network, it must be able to supply the loads without any load curtailment. In this regard, a Diesel-Generator can also be integrated to achieve zero loss of load. The optimal hybrid system design problem is a discrete optimization problem that is solved, here, by means of a recently-introduced meta-heuristic optimization algorithm known as Lightning Attachment Procedure Optimization (LAPO). The results are compared to those of some other methods and discussed in detail. The results also show that the total cost of the designed stand-alone system in 25 years is around 92M€ which is much less than the grid-connected system with the total cost of 205M€. In summary, the obtained simulation results demonstrate the effectiveness of the utilized optimization algorithm in finding the best results, and the designed hybrid system in serving the remote loads.

• 한국기계가공학회지
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• 제14권1호
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• pp.70-77
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• 2015

This paper develops and evaluates a mechanical machining process which involves drilling on PVS material. According to the material, two treatment experiments were conducted, one involving drilling in a wet condition or using a lubricant and one involving drilling in a dry condition with no lubricant. Drilling in a dry condition showed better performance in terms of the cutting time than in the wet condition. Otherwise, the wet condition has several advantages. The lubricant influenced the burr diameter size and minimized the temperature on the surface of the work piece. During the wet condition drilling process, a smaller burr diameter size was noted as compared to the dry condition. The temperature showed a linear correlation with the drill bit size, where a least-square analysis provided an $R^2$valuewhichexceeded 0.95. The wet condition required more cutting time than the dry condition. In this condition, the water provides a lubrication effect. A thin layer between the cutting edges and the surface of the work piece is formed. The chip formation is affected by the drilling depth. The color on the tips of the chips was darker than in the initial condition. No correlation between the drilling depth and the bore roughness was noted, but the variation of the cutting speed or the RPM influenced the roughness of the bore. The optimum cutting speed ranged from 40 RPM to 45 RPM in the condition which provided the finest roughness surface.

• 대한전자공학회논문지SP
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• 제40권6호
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• pp.114-123
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• 2003

본 논문에서는 다각형 기반 윤곽선 부호화를 위한 새로운 정점 선택 기법을 제안한다. 객체의 모양을 효율적으로 표현하기 위해서 정점 선택 과정에서 기존의 최대 거리 척도뿐만 아니라 곡률 정보를 이용한다. 제안된 기법은 두 단계 과정으로 구성되어 있다. 첫 번째 단계에서는 곡률이 큰 윤곽선 화소들을 CSS (curvature scale space)를 이용하여 키 정점(key vortices)으로 선택한다. 이 점들은 객체의 윤곽선을 여러 개의 부분 윤곽선(contour segment)들로 나누며, 각 부분 윤곽선은 양 끝점이 인접한 두 개의 키 정점인 하나의 열린 윤곽선으로 독립적으로 처리된다. 두 번째 단계에서는 각각의 부분 윤곽선에서 주어진 허용 오차(D/sub max//sup */)를 만족하면서 최소 개수의 정점을 선택하기 위해 순차적 정점 선택 기법을 이용하여 정점을 선택한다. 그리고 선택된 정점들은 오차 영역 면에서 최적의 위치를 찾기 위해 동적 프로그래밍(DP : dynamic programming) 기법을 사용하여 조정된다. 제안된 기법과 기존의 기법들의 근사 성능을 비교하기 위해 실험 결과를 제시한다.

• Parasites, Hosts and Diseases
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• 제51권3호
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• pp.279-287
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• 2013

Autophagy is a process of cytoplasmic degradation of endogenous proteins and organelles. Although its primary role is protective, it can also contribute to cell death. Recently, autophagy was found to play a role in the activation of host defense against intracellular pathogens. The aims of our study was to investigate whether host cell autophagy influences Toxoplasma gondii proliferation and whether autophagy inhibitors modulate cell survival. HeLa cells were infected with T. gondii with and without rapamycin treatment to induce autophagy. Lactate dehydrogenase assays showed that cell death was extensive at 36-48 hr after infection in cells treated with T. gondii with or without rapamycin. The autophagic markers, LC3 II and Beclin 1, were strongly expressed at 18-24 hr after exposure as shown by Western blotting and RT-PCR. However, the subsequent T. gondii proliferation suppressed autophagy at 36 hr post-infection. Pre-treatment with the autophagy inhibitor, 3-methyladenine (3-MA), down-regulated LC3 II and Beclin 1. The latter was also down-regulated by calpeptin, a calpain inhibitor. Monodansyl cadaverine (MDC) staining detected numerous autophagic vacuoles (AVs) at 18 hr post-infection. Ultrastructural observations showed T. gondii proliferation in parasitophorous vacuoles (PVs) coinciding with a decline in the numbers of AVs by 18 hr. FACS analysis failed to confirm the presence of cell apoptosis after exposure to T. gondii and rapamycin. We concluded that T. gondii proliferation may inhibit host cell autophagy and has an impact on cell survival.