• Parasites, Hosts and Diseases
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• 제46권4호
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• pp.209-216
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• 2008

A monoclonal antibody against Toxoplasma gondii of Tg556 clone (Tg556) blotted a 29 kDa protein, which was localized in the dense granules of tachyzoites and secreted into the parasitophorous vacuolar membrane (PVM) after infection to host cells. A cDNA fragment encoding the protein was obtained by screening a T. gondii cDNA expression library with Tg556, and the full-length was completed by 5'-RACE of 2,086 bp containing an open reading frame (ORF) of 669 bp. The ORF encoded a polypeptide of 222 amino acids homologous to the revised GRA3 but not to the first reported one. The polypeptide has 3 hydrophobic moieties of an N-terminal stop transfer sequence and 2 transmembrane domains (TMD) in posterior half of the sequence, a cytoplasmic localization motif after the second TMD and an endoplasmic reticulum (ER) retrival motif in the C-terminal end, which suggests GRA3 as a type III transmembrane protein. With the ORF of GRA3, yeast two-hybrid assay was performed in HeLa cDNA expression library, which resulted in the interaction of GRA3 with calcium modulating ligand (CAMLG), a type II transmembrane protein of ER. The specific binding of GRA3 and CAMLG was confirmed by glutathione S-transferase (GST) pull-down and immunoprecipitation assays. The localities of fluorescence transfectionally expressed from GRA3 and CAMLG plasmids were overlapped completely in HeLa cell cytoplasm. In immunofluorescence assay, GRA3 and CAMLG were shown to be co-localized in the PVM of host cells. Structural binding of PVM-inserted GRA3 to CAMLG of ER suggested the receptor-ligand of ER recruitment to PVM during the parasitism of T. gondii.

• 한국시뮬레이션학회논문지
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• 제5권2호
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• pp.13-24
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• 1996

Workflow is an ordered sequence of interdependent component data activities each of which can be executed on an integrated information system by accessing a remote information system. In our previous research [4], we proposed a distributed CIM Workflow system which consists of a workflow execution model called DAF-Net and an agent-based information systems called AIMIS. Given a component data activity, there needs an interaction protocol among agents which allocates the component data activity to a relevant information systems exist. The objective of this research is to propose and test two protocols: ARR(Asynchronous Request and Response)protocol and NCL(Negotiation with Case based Learning) protocol. To test the effectiveness of the protocols, we applied the PVM(Parallel Virtual Machine) software to simulate the distributed CIM Workflow system. PVM provides a distributed computing environment in which users can run different software processes in different computers while allowing communication among the processes.

• Journal of applied mathematics & informatics
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• 제6권2호
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• pp.649-659
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• 1999

This paper presents a parallel implementation of stiff-ness matrix calculation based on the processor farm model on a net-work of workstations running PVM programming environment. As the computational characteristics of stiffnes matrix exhibits good po-tentials for effective prallel computation the performance improve-ment is show to be almost linear with the number of sorkstations involved in the computation.

• 한국정보과학회논문지:시스템및이론
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• 제29권7호
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• pp.377-383
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• 2002

외판원 문제는 잘 알려진 NP-완전 문제로, 최적해(optimal value)를 구하는 다양한 알고리즘들이 개발되었다. 그러나 최악의 경우 지수 시간이 걸리므로 수행시간을 줄이는 다양한 방법들이 제안되고 있다. 최근접 휴리스틱 알고리즘은 최적해를 구하는 다른 알고리즘들에 비해 구조가 비교적 간단하다. 따라서 본 논문에서는 외판원 문제(Traveling Salesman Problem, TSP)의 최적해를 구할 수 있는 분기 함수(bounding function)를 적용한 분산 최근접 휴리스틱(nearest neighbor heuristic) 알고리즘을 PVM(Parallel Virtual Machine)에서 제공하는 마스터/슬래이브(master/slave) 모델을 사용하여 설계하고 구현하였다. 먼저 최적해를 찾는 수행 시간을 줄이기 위해 최적화 문제에서 좋은 성능을 보이는 분산 유전 알고리즘(distributed genetic algorithm)을 수행해 얻은 근사해(near optimal)를 초기 분기 함수로 사용한다. 특히 더욱 좋은 근사해를 구하고자 유전 연산자인 돌연변이를 새롭게 변형하여 적용하였다.

• 한국통신학회논문지
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• 제30권3B호
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• pp.105-113
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• 2005

본 연구에서는 PVM으로 구성된 클러스터의 공유를 기반으로 하는 단계화된 응용서비스 플랫폼을 기반으로 응용서비스를 구현함으로써 응용서비스 플랫폼의 유용성을 평가하였다. 응용서비스 플랫폼은 요청 처리에 필요한 전 과정을 요청처리 단계와 서비스제공 단계로 나누고, 서비스요청처리 단계를 위한 전위응용서버의 서비스관리자와 요청된 서비스의 분산을 담당하는 부하관리자, 그리고 후위서버에서의 서비스제공을 위한 작업관리자로 구성된다. 구현된 응용서비스 플랫폼은 필요한 처리자원을 동적으로 할당, 시스템을 재구성함으로써 기존의 단일서버 시스템에 비해 부하의 변화에 보다 능동적으로 대처할 수 있을 뿐 아니라, 기능의 변경없이 다양한 응용서비스의 구현을 지원함을 확인하였다.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2000년도 제15차 학술회의논문집
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• pp.8-8
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• 2000

PICARD (Port-Interface Component Architecture for Real-time system Design) is a software architecture and environment, which is aimed to reduce development time and cost of real-time, control system. With PICARD, a control engineer can construct a control system software by assembling pre-built software components us ing interact ive graphical development environment. PICARD consists of PVM(Picard Virtual Machine) , a component library, and PICE(PIcard Configuration Editor). PVM is a real-time engine of the PICARD system which runs control tasks on a real-time operating system. The component library is composed of components which are called task blocks. PICE is a visual editor which can configure control tasks by creating data-flow diagrams of task blocks or Ladder diagrams for sequential logics. For the communication between PVM on a target system and PICE on a host computer, a simple protocol and tools for stub generation was dove]oped because RPC or CORBA is difficult to be applied for the embedded system. New features such as a byte-code based run time system and a simple and easy MMI builder are also introduced.

• 정보과학회지
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• 제13권7호
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• pp.115-129
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• 1995

• 전산구조공학
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• 제11권3호
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• pp.78-84
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• 1998

• 대한전기학회:학술대회논문집
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• 대한전기학회 1995년도 추계학술대회 논문집 학회본부
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• pp.12-14
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• 1995

전기기계의 설계시에 얻어지는 대형 선형방정식을 풀기 위해서는 엄청난 메모리가 필요하고 계산시간이 요구된다는 점을 고려해야 한다. 병렬계산은 이러한 문제를 해결할 수 있다. 우리는 PVM 환경에서 병렬계산이 가능한 솔비를 설계하였다. 선형방정식을 푸는 방법으로 QMR을 이용하였으며, 수렴속도를 향상시키기 위하여 대각 프리컨디셔너를 사용하였다. 실행 결과는 본 논문에서 구현한 솔비가 실제로 발생하는 문제에 대하여 아주 훌륭하게 동작하는 것을 보여준다.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2007년도 하계종합학술대회 논문집
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• pp.137-138
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• 2007

This paper describes a design methodology of wireless networked Public View Monitor (PVM) based on Bluetooth network. We present the S/W design methodology of wireless networked PVM and experimental analysis using the implemented system. The developed system can be applied to industrial and market area by easy maintenance and flexibility.